A comprehensive DNA barcoding of Indian freshwater fishes of the Indus River system, Beas.

The Beas River is one of the important rivers of the Indus River system located in Himachal Pradesh, India, that harbors a diverse range of freshwater fish species. The present study employed COI gene to investigate the ichthyofaunal diversity of river Beas. Through the sequencing of 203 specimens from Beas River, we identified 43 species, belonging to 31 genera, 16 families, and 10 orders. To analyze the genetic divergence and phylogeny of identified species, 485 sequences of Indian origin were retrieved from BOLD, resulting in a dataset of 688 sequences. Our findings consistently revealed a hierarchical increase in the mean K2P genetic divergence within species (0.80%), genus (9.06%), and families (15.35%). Automated Barcode Gap discovery, Neighbour Joining, and Bayesian inference consensus tree methodologies were employed to determine the putative species and their phylogeny, successfully delimiting most of the species with only a few exceptions. The results unveiled six species exhibiting high intra-species divergence (> 2%), suggesting the presence of sibling species and falsely identified sequences on online databases. The present study established the first DNA barcoding-based inventory of freshwater fish species in the Beas River providing comprehensive insights into economically exploited endangered and vulnerable species. In order to ensure the sustainable use of aquatic resources in the Beas River, we recommend the implementation of species measures to protect biodiversity and genetic resources.

Unveiling the ecotoxicological impact of microplastics on organisms - the persistent organic pollutant (POP): A comprehensive review.

Microplastics have been ubiquitous in our environment for decades, and numerous studies have revealed their extensive dispersion, reaching far beyond the surface of the land, soil, aquatic ecosystems. They have infiltrated the food-chain, the food web, even the air we breathe, as well as the water we drink. Microplastics have been detected in the food we consume, acting as vectors for hazardous chemicals that adhere to their hydrophobic surfaces. This can result in the transfer of these chemicals to the aquatic life, posing a threat to their well-being. The release of microplastics into different environmental settings can give rise to various eco-toxicological implications. The substantial body of literature has led scientists to the consensus that microplastic pollution is a global problem with the potential to impact virtually any type of ecosystem. This paper aims to discuss crucial information regarding the occurrence, accumulation, and ecological effects of microplastics on organisms. It also highlights the new and emerging disease named "Plasticosis" that is directly linked to microplastics and its toxicological effects like permanent scarring and long-term inflammation in the digestive system of the seabirds. By comprehending the behaviour of these microplastic pollutants in diverse habitats and evaluating their ecological consequences, it becomes possible to facilitate a better understanding of this toxicological issue.