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1.
Langmuir ; 40(14): 7733-7746, 2024 04 09.
Artículo en Inglés | MEDLINE | ID: mdl-38538620

RESUMEN

The mechanism of ethanol-induced fibrillation of ß-lactoglobulin (ß-lg) in the acidic aqueous solution upon heating was investigated using various techniques, mainly thioflavin T fluorescence, atomic force microscopy, nonreducing electrophoresis, mass spectrometry, Fourier transform infrared spectroscopy, and circular dichroism spectroscopy. The results showed that fibrillation occurred with a heating time increase, but high ethanol content slowed down the process. At a low ethanol volume fraction, peptides existed after heating for 2 h, with long and straight fibrils formed after 4-6 h, while at a high ethanol volume fraction, the proteins aggregated with very few peptides appeared at the early stage of heating, and short and curved fibrils formed after heating for 8 h. Ethanol weakened the hydrophobic interactions between proteins in the aqueous solution; therefore the latter could not completely balance the electrostatic repulsion, and thus suppressing the fibrillation process. It is believed that the fibrillation of ß-lg in the acidic solution upon heating is mainly dominated by the polypeptide model; however, ethanol inhibited the hydrolysis of proteins, and the self-assembly mechanism changed to the monomer model.


Asunto(s)
Lactoglobulinas , Agua , Solventes/química , Lactoglobulinas/química , Péptidos , Etanol , Espectroscopía Infrarroja por Transformada de Fourier , Microscopía de Fuerza Atómica , Dicroismo Circular
2.
Pestic Biochem Physiol ; 204: 106075, 2024 Sep.
Artículo en Inglés | MEDLINE | ID: mdl-39277388

RESUMEN

Currently, the primary strategy for tick control relies on chemical agents. Pyrethrins, which are botanically derived compounds, have demonstrated efficacy in controlling ticks without posing a risk to human or animal health. However, research into pyrethrins' metabolic mechanisms remains sparse. Cystatin, as a reversible binding inhibitor of cysteine protease, may be involved in the initiation of pyrethrin detoxification of Haemaphysalis doenitzi. In this study, two novel cystatins were cloned, HDcyst-3 and HDcyst-4, the relative expression of which was highest in the Malpighian tubules compared with the tick midguts, salivary glands, and ovaries. Prokaryotic expression and in vitro studies revealed that cystatins effectively inhibit the enzymatic activities of cathepsins B and S. RNAi results showed that the reduction of cystatins significantly decreased the engorgement weight, egg mass weight, and egg hatching rate of adult female ticks, and prolonged feeding time by two days. The control rate of rHDcyst-3 and rHDcyst-4 protein vaccination against female adults were 55.9% and 63.2%, respectively. In addition, the tick immersion test showed that cypermethrin and λ-cyhalothrin had significant acaricidal effects against adult unfed H. doenitzi. The qPCR result indicated that compared with the control group, the expression of HDcyst-3 and HDcyst-4 was markedly decreased in the sublethal cypermethrin and λ-cyhalothrin group at LC50. Enzyme activity showed that cypermethrin and λ-cyhalothrin could significantly induce the activities of glutathione S-transferase (GST), carboxylesterase (CarE), and acetylcholinesterase (AchE). The aforementioned results provided indirect evidence that cystatin plays an important role in pyrethrin detoxification and provides a theoretical basis for future acaricide experiments and pest management.


Asunto(s)
Cistatinas , Nitrilos , Piretrinas , Piretrinas/farmacología , Animales , Nitrilos/farmacología , Cistatinas/metabolismo , Cistatinas/genética , Femenino , Ixodidae/efectos de los fármacos , Ixodidae/genética , Ixodidae/metabolismo , Acaricidas/farmacología , Resistencia a Medicamentos
3.
Pestic Biochem Physiol ; 203: 105992, 2024 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-39084793

RESUMEN

Haemaphysalis longicornis, which is widely distributed in China, can transmit various tick-borne diseases such as severe fever with thrombocytopenia syndrome, babesiosis, rickettsia disease and so on, and do great harm to human health and the development of animal husbandry. Chemical acaricides are the most traditional tick control method, but because of its many shortcomings, there is an urgent need to find a substitute with high efficiency, environmental protection and low toxicity. It has been found that some plant essential oils (EOs) have good insecticidal activity and environmental safety. In this study, the components of EOs from Pimenta racemosa and Eugenia caryophyllata were analyzed by gas chromatography-mass spectrometry (GC-MS), and their potential for application in the control of Haemaphysalis longicornis were studied. Gas chromatography-mass spectrometry analysis showed that the main components of P. racemosa EO were eugenol (64.07%), those of E. caryophyllata EO were Hexadecanoic acid, 2-methylpropyl ester (51.84%) and eugenol (39.76%). Larval packet test showed that the EOs of P. racemosa and E. caryophyllata had significant acaricidal activity against unfed larvae of H. longicornis, with LC50 values of 1.20 mg/mL and 0.47 mg/mL and LC90 values of 8.76 mg/mL and 2.91 mg/mL, respectively. The P. racemosa EO, E. caryophyllata EO and eugenol showed significant acaricidal activity against unfed nymph H. longicornis, with LC50 values of 1.65 mg/mL, 2.29 mg/mL and 0.93 mg/mL and LC90 values of 5.03 mg/mL, 11.01 mg/mL and 4.77 mg/mL, respectively. The P. racemosa EO, E. caryophyllata EO and eugenol showed significant acaricidal activity against unfed adults H. longicornis, with LC50 values of 0.51 mg/mL, 2.57 mg/mL and 1.83 mg/mL and LC90 values of 2.44 mg/mL, 11.44 mg/mL and 2.54 mg/mL, respectively. Enzyme assays revealed that the E. caryophyllata EO and eugenol significantly inhibited the activity of carboxylesterase (CarE), eugenol significantly inhibited the activity of catalase (CAT), and two EOs and eugenol had no significant effect on acetylcholinesterase (AchE) (p < 0.05). The above results suggest that the essential oils from P. racemosa and E. caryophyllata have great potential for use as alternatives to synthetic acaricides for tick control.


Asunto(s)
Acaricidas , Eugenia , Ixodidae , Larva , Aceites Volátiles , Pimenta , Animales , Aceites Volátiles/farmacología , Aceites Volátiles/química , Ixodidae/efectos de los fármacos , Acaricidas/farmacología , Eugenia/química , Pimenta/química , Larva/efectos de los fármacos , Cromatografía de Gases y Espectrometría de Masas , Aceites de Plantas/farmacología , Aceites de Plantas/química , Eugenol/análogos & derivados , Eugenol/farmacología , Haemaphysalis longicornis
4.
Sensors (Basel) ; 24(16)2024 Aug 18.
Artículo en Inglés | MEDLINE | ID: mdl-39205036

RESUMEN

The dispute over the authenticity of video has become a hot topic in judicial practice in recent years. Despite detection methods being updated rapidly, methods for determining authenticity have limitations, especially against high-level forgery. Deleting the integral group of pictures (GOP) length in static scenes could remove key information in the video, leading to unjust sentencing. Anyone can conduct such an operation using publicly available software, thus escaping state-of-the-art detection methods. In this paper, we propose a detection method based on noise transfer matrix analysis. A pyramid structure and a weight learning module are adopted to improve the detection rate and reduce the false positive rate. In total, 80 videos were examined through delicate anti-forensic forgery operations to verify the detection performance of the proposed method and three previously reported methods against anti-forensic forgery operations. In addition, two of the latest learning-based methods were included in our experiments to evaluate the proposed method. The experimental results show that the proposed method significantly improves the detection of frame deletion points compared with traditional and learning-based methods, especially in low false positive rate (FPR) intervals, which is meaningful in forensic science.

5.
J Obstet Gynaecol ; 44(1): 2338235, 2024 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-38619096

RESUMEN

BACKGROUND: Selection of high-quality blastocysts is the most important factor determining the success of assisted reproductive technology. The objective of this study is to assess the values of blastocyst morphological quality and development speed for predicting euploidy and clinical pregnancy outcome. METHODS: A total of 155 preimplantation genetic testing cycles including 959 blastocysts and 154 euploid blastocyst transfer cycles conducted between January 2018 and December 2019 were retrospectively analysed. The associations of blastocyst morphological quality and development speed (D) with chromosomal status, clinical pregnancy rate, early miscarriage rate, and ongoing pregnancy rate were evaluated by univariate and multivariate regression. RESULTS: The euploidy rate of development speed D5 blastocysts was significantly greater than that of D6 blastocysts (61.4% vs. 38.1%, P < 0.001), and the euploid rate of morphologically high-grade blastocysts was significantly greater than that of non-high-grade blastocysts. Development speed D5 (OR = 1.6, 95% CI 1.2-2.2, P = 0.02) and high-grade morphology (OR = 2.1, 95% CI 1.5-2.9, P = 0.01) were independent predictors of euploidy. The ongoing pregnancy rate of D5 blastocysts was significantly higher than that of D6 blastocysts (62.3% vs. 43.8%, P = 0.04). Transfer of euploid blastocysts with high-grade morphology resulted in a greater ongoing pregnancy rate than transfer of non-high-grade euploid blastocysts (60.7% vs. 43.2%, P = 0.049). Alternatively, D6 development speed was an independent risk factor for early pregnancy loss after euploid blastocyst transfer. Multivariate regression analysis adjusting for confounding factors identified maternal age, blastocyst development speed, and blastocyst morphological grade as independent predictors of euploidy but not of clinical pregnancy. CONCLUSION: The recommended sequence of embryo transfer based on the present study is D5 high-grade > D6 high-grade > D5 non-high-grade > D6 non-high-grade.


Assisted reproductive technology physicians are actively exploring methods to improve the accuracy of embryo selection for successful pregnancy. We evaluated the associations of embryo morphological grade and development speed with chromosomal status and clinical outcome for couples without a history of infertility, in vitro fertilisation failure, or recurrent miscarriage receiving euploid embryo transfer. Blastocysts from females younger than 35 years, of high morphological grade, and demonstrating faster development speed were most likely to be euploid (least likely to have chromosomal abnormalities). Alternatively, patients implanted with slower developing euploid blastocysts were at higher risk of early pregnancy loss. To maximise the probability of implanting euploid embryos and minimise the risk of pregnancy loss, the selection order of embryo transferred should be based on embryo development speed followed by morphological grades.


Asunto(s)
Aborto Espontáneo , Resultado del Embarazo , Embarazo , Femenino , Humanos , Resultado del Embarazo/epidemiología , Transferencia de un Solo Embrión , Estudios Retrospectivos , Blastocisto , Embrión de Mamíferos , Aborto Espontáneo/epidemiología , Aborto Espontáneo/etiología
6.
J Xray Sci Technol ; 27(5): 981-993, 2019.
Artículo en Inglés | MEDLINE | ID: mdl-31450541

RESUMEN

OBJECTIVE: To compare image quality, radiation dose, and iodine intake of head-neck CT angiography (CTA) acquired by wide-detector with the gemstone spectral imaging (GSI) combination with low iodine intake or routine scan protocol. METHODS: Three hundred patients who had head-neck CTA were enrolled and divided into three groups according to their BMI values: group A (18.5 kg/m2 ≦ BMI <24.9 kg/m2), group B (24.9 kg/m2 ≦ BMI <29.9 kg/m2) and group C (29.9 kg/m2 ≦ BMI ≦ 34.9 kg/m2) with 100 patients in each group. Patients in each group were randomly divided into two subgroups (n = 50) namely, A1, A2, B1, B2, C1 and C2. The patients in subgroups A1, B1 and C1 underwent GSI with low iodine intake (270 mgI/ml, 50 ml) and combined with the ASiR-V algorithm. Other patients underwent three dimensional (3D) smart mA modulation with routine iodine intake (350 mgI/ml, 60 ml). Signal-to-noise ratio (SNR) and contrast-to-noise ratio (CNR) of all images were calculated after angiography. Images were then subjectively assessed using a 5-point scale. CT dose index of volume and dose-length product (DLP) was converted to the effective dose (ED) and then compared. RESULTS: The mean CT values, SNR, CNR and subjective image quality in subgroups A2, B2 and C2 are significantly lower than in subgroups A1, B1, and C1 (P < 0.01), respectively. The ED values in subgroup A1, B1, and C1 are 55.18%, 61.89%, and 69.64% lower than those in A2, B2, and C2, respectively (P < 0.01). The total iodine intakes in subgroups A1, B1, and C1 are 35.72% lower than those in subgroups A2, B2, and C2. CONCLUSIONS: The gemstone spectral imaging with monochromatic images at 53-57 keV combined with ASiR-V algorithm allows significant reduction in iodine load and radiation dose in head-neck CT angiography than those yielded in routine scan protocol. It also enhances signal intensity of head-neck CTA and maintains image quality.


Asunto(s)
Angiografía por Tomografía Computarizada/métodos , Cabeza/diagnóstico por imagen , Cuello/diagnóstico por imagen , Interpretación de Imagen Radiográfica Asistida por Computador/métodos , Adulto , Anciano , Algoritmos , Índice de Masa Corporal , Medios de Contraste/administración & dosificación , Femenino , Humanos , Yodo/administración & dosificación , Masculino , Persona de Mediana Edad , Dosis de Radiación , Relación Señal-Ruido
7.
Front Immunol ; 15: 1374465, 2024.
Artículo en Inglés | MEDLINE | ID: mdl-39119345

RESUMEN

Background: Increasing evidence have highlighted the biological significance of mRNA N6-methyladenosine (m6A) modification in regulating tumorigenicity and progression. However, the potential roles of m6A regulators in tumor microenvironment (TME) formation and immune cell infiltration in liver hepatocellular carcinoma (LIHC or HCC) requires further clarification. Method: RNA sequencing data were obtained from TCGA-LIHC databases and ICGC-LIRI-JP databases. Consensus clustering algorithm was used to identify m6A regulators cluster subtypes. Weighted gene co-expression network analysis (WGCNA), LASSO regression, Random Forest (RF), and Support Vector Machine-Recursive Feature Elimination (SVM-RFE) were applied to identify candidate biomarkers, and then a m6Arisk score model was constructed. The correlations of m6Arisk score with immunological characteristics (immunomodulators, cancer immunity cycles, tumor-infiltrating immune cells (TIICs), and immune checkpoints) were systematically evaluated. The effective performance of nomogram was evaluated using concordance index (C-index), calibration plots, decision curve analysis (DCA), and receiver operating characteristic curve (ROC). Results: Two distinct m6A modification patterns were identified based on 23 m6A regulators, which were correlated with different clinical outcomes and biological functions. Based on the constructed m6Arisk score model, HCC patients can be divided into two distinct risk score subgroups. Further analysis indicated that the m6Arisk score showed excellent prognostic performance. Patients with a high m6Arisk score was significantly associated with poorer clinical outcome, lower drug sensitivity, and higher immune infiltration. Moreover, we developed a nomogram model by incorporating the m6Arisk score and clinicopathological features. The application of the m6Arisk score for the prognostic stratification of HCC has good clinical applicability and clinical net benefit. Conclusion: Our findings reveal the crucial role of m6A modification patterns for predicting HCC TME status and prognosis, and highlight the good clinical applicability and net benefit of m6Arisk score in terms of prognosis, immunophenotype, and drug therapy in HCC patients.


Asunto(s)
Adenosina , Biomarcadores de Tumor , Carcinoma Hepatocelular , Neoplasias Hepáticas , Microambiente Tumoral , Humanos , Carcinoma Hepatocelular/inmunología , Carcinoma Hepatocelular/genética , Carcinoma Hepatocelular/patología , Microambiente Tumoral/inmunología , Microambiente Tumoral/genética , Neoplasias Hepáticas/inmunología , Neoplasias Hepáticas/genética , Neoplasias Hepáticas/patología , Neoplasias Hepáticas/mortalidad , Neoplasias Hepáticas/diagnóstico , Pronóstico , Biomarcadores de Tumor/genética , Adenosina/análogos & derivados , Adenosina/metabolismo , Nomogramas , Regulación Neoplásica de la Expresión Génica , Perfilación de la Expresión Génica , Femenino , Transcriptoma , Masculino
8.
Pest Manag Sci ; 80(8): 3957-3966, 2024 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-38521986

RESUMEN

BACKGROUND: Ticks, which are obligate blood-feeding parasites, transmit a wide range of pathogens during their hematophagic process. Certain enzymes and macromolecules play a crucial role in inhibition of several tick physiological processes, including digestion and reproduction. In the present study, genes encoding type 2 cystatin were cloned and characterized from Haemaphysalis doenitzi, and the potential role of cystatin in tick control was further assessed. RESULTS: Two cystatin genes, HDcyst-1 and HDcyst-2, were successfully cloned from the tick H. doenitzi. Their open reading frames are 390 and 426 base pairs, and the number of coding amino acids are 129 and 141, respectively. In the midgut, salivary glands, Malpighian tubules and ovaries of ticks, the relative expression of HDcyst-1 was higher in the midgut and Malpighian tubules, and HDcyst-2 was higher in the salivary glands of H. doenitzi, respectively. Lipopolysaccharide (LPS) injection and low-temperature stress elevated cystatin expression in ticks. Enzyme-linked immunosorbent assay showed that both rHDcyst-1 and rHDcyst-2 protein vaccines increased antibody levels in immunized rabbits. A vaccination trial in rabbits infected with H. doenitzi showed that both recombinant cystatin proteins significantly reduced tick engorgement weights and egg mass weight, in particular, rHDcyst-1 significantly prolonged tick engorgement time by 1 day and reduced egg hatching rates by 16.9%. In total, rHDcyst-1 and rHDcyst-2 protein vaccinations provided 64.1% and 51.8% protection to adult female ticks, respectively. CONCLUSION: This is the first report on the immunological characterization of the cystatin protein and sequencing of the cystatin gene in H. doenitzi. Cystatin proteins are promising antigens that have the potential to be used as vaccines for infestation of H. doenitzi control. © 2024 Society of Chemical Industry.


Asunto(s)
Proteínas de Artrópodos , Frío , Cistatinas , Ixodidae , Vacunas , Animales , Cistatinas/genética , Conejos , Femenino , Vacunas/inmunología , Ixodidae/inmunología , Ixodidae/fisiología , Ixodidae/genética , Proteínas de Artrópodos/genética , Proteínas de Artrópodos/metabolismo , Proteínas de Artrópodos/inmunología , Estrés Fisiológico , Lipopolisacáridos/farmacología , Secuencia de Aminoácidos
9.
Nat Biotechnol ; 2024 Mar 12.
Artículo en Inglés | MEDLINE | ID: mdl-38472508

RESUMEN

Tumor genomes often harbor a complex spectrum of single nucleotide alterations and chromosomal rearrangements that can perturb protein function. Prime editing has been applied to install and evaluate genetic variants, but previous approaches have been limited by the variable efficiency of prime editing guide RNAs. Here we present a high-throughput prime editing sensor strategy that couples prime editing guide RNAs with synthetic versions of their cognate target sites to quantitatively assess the functional impact of endogenous genetic variants. We screen over 1,000 endogenous cancer-associated variants of TP53-the most frequently mutated gene in cancer-to identify alleles that impact p53 function in mechanistically diverse ways. We find that certain endogenous TP53 variants, particularly those in the p53 oligomerization domain, display opposite phenotypes in exogenous overexpression systems. Our results emphasize the physiological importance of gene dosage in shaping native protein stoichiometry and protein-protein interactions, and establish a framework for studying genetic variants in their endogenous sequence context at scale.

10.
J Colloid Interface Sci ; 629(Pt A): 276-286, 2023 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-36081207

RESUMEN

S-scheme heterojunction structure can endow the photocatalysts with high-performance photo-degradation of pharmaceuticals and personal care products (PPCPs) since it can remain the photogenerated electrons/holes with stronger redox ability. Herein, an integrative S-scheme heterojunction photocatalyst building from Cd0.5Zn0.5S nanoparticles and BiOCl microflowers with oxygen vacancies (OVs) was developed. Moreover, the in-situ grown process ensures the firm contact and intense electron coupling between BiOCl and Cd0.5Zn0.5S. As a result, Cd0.5Zn0.5S/BiOCl exhibited a significant reinforcement of photo-activity and stability for the abatement of antibiotic norfloxacin, manifesting a 2.8-fold or 9.6-fold enhancement compared to pristine Cd0.5Zn0.5S or BiOCl. Cd0.5Zn0.5S/BiOCl also shows good resistance to alkaline, sodium salts and humic acid. The performance of Cd0.5Zn0.5S/BiOCl to photocatalytically degrade other PPCPs with different molecular structures was further confirmed. At last, the ability of Cd0.5Zn0.5S/BiOCl for PPCPs de-toxicity was verified by evaluating the toxicity of norfloxacin and its degradation intermediate. This study demonstrates a new S-scheme heterojunction photocatalyt for efficient removal of PPCPs as well as provides some insights into developing high-performance metal sulfide solid-solution-based S-scheme heterojunctions for water decontamination.


Asunto(s)
Cadmio , Norfloxacino , Fotólisis , Antibacterianos/farmacología , Antibacterianos/química , Catálisis , Oxígeno , Sustancias Húmicas , Sales (Química) , Luz , Zinc , Sulfuros , Agua , Preparaciones Farmacéuticas , Sodio
11.
AJOG Glob Rep ; 3(2): 100201, 2023 May.
Artículo en Inglés | MEDLINE | ID: mdl-37168544

RESUMEN

BACKGROUND: Recent studies have reported higher risks of adverse maternal and neonatal outcomes, such as hypertensive disorders of pregnancy, after programmed frozen embryo transfer, especially in cycles with gonadotropin-releasing hormone agonist pretreatment. It remains unclear if GnRH agonist pretreatment before programmed frozen embryo transfer further increases the risk for obstetrical complications among women with polycystic ovary syndrome. OBJECTIVE: This study aimed to compare the obstetrical and neonatal complications of singleton and twin pregnancies after programmed frozen embryo transfer with or without gonadotropin-releasing hormone-a pretreatment among women with polycystic ovary syndrome. STUDY DESIGN: This was a secondary analysis of a single-center, randomized controlled trial comparing the live birth rate and cost-effectiveness of programmed cycle-prepared frozen embryo transfers with or without gonadotropin-releasing hormone agonist pretreatment among women with polycystic ovary syndrome. The analysis was restricted to women with polycystic ovary syndrome, aged 24 to 40 years, who delivered live neonates after frozen-thawed blastocyst transfer. The obstetrical and neonatal outcomes were compared between programmed cycles with and those without gonadotropin-releasing hormone agonist pretreatment. The adjusted odds ratios with 95% confidence intervals were calculated and adjustments were made for relevant confounders. RESULTS: The maternal and neonatal complications associated with 177 live single births and 38 twin births (253 newborns in total) were analyzed. There were no significant differences in the frequencies of obstetrical complications, including hypertensive disorders of pregnancy, between the gonadotropin-releasing hormone agonist pretreatment and no pretreatment group for both singleton and twin pregnancies. However, there was a significantly greater incidence of having a low birthweight neonate among singleton infants born after gonadotropin-releasing hormone agonist pretreatment when compared with no pretreatment (10.2% vs 1.3%; P=0.042), and a low birthweight among singleton infants was still more likely after adjusting for confounders (relative ratio, 3.85; 95% confidence interval, 1.13-7.11; P=.024). Other neonatal complications were all comparable between the pretreatment and no pretreatment groups for both singleton and twin pregnancies. CONCLUSION: For women with polycystic ovary syndrome, programmed frozen embryo transfer cycles with gonadotropin-releasing hormone agonist pretreatment could lead to a greater risk of having a low birthweight singleton neonate.

12.
J Cancer Res Clin Oncol ; 149(13): 12249-12263, 2023 Oct.
Artículo en Inglés | MEDLINE | ID: mdl-37434092

RESUMEN

BACKGROUND: Copper homeostasis imbalance has been implicated in tumor progression, aggressiveness, and treatment response. However, the precise roles of cuproptosis-related genes (CRGs) in hepatocellular carcinoma (HCC) remain poorly understood. METHODS: In this study, we employed a consensus clustering algorithm to identify distinct molecular subtypes. We then performed Kaplan-Meier analysis and univariate Cox regression analysis to identify prognostic differentially expressed genes. The expression of these genes was subsequently validated using qPCR on fresh-frozen tissues obtained from HCC patients. Moreover, leveraging the TCGA-HCC cohort, we constructed a CRGs-related risk prediction model using the LASSO and multivariate Cox regression analysis. RESULTS: By analyzing the data, we successfully established a CRGs risk prognostic model for HCC patients, comprising five differential genes (CAD, SGCB, TXNRD1, KDR, and MTND4P20). Cox regression analysis revealed that the CRGs risk score could serve as an independent prognostic factor for overall survival (hazard ratio [HR] = 1.308, 95% confidence interval [CI] = 1.200 - 1.426, P < 0.001). The area under the curve (AUC) values of the CRGs-score for predicting 1-year, 3-year, and 5-year survival rates were 0.785, 0.724, and 0.723, respectively. Notably, the expression levels of immune checkpoints (including PD-1, PD-L1, and CTLA4) significantly differed between the low- and high-risk score groups. Furthermore, the low-risk score group displayed increased sensitivity to sorafenib, cisplatin, cyclopamine, nilotinib, salubrinal, and gemcitabine, whereas the high-risk score group exhibited heightened sensitivity to lapatinib, erlotinib, and gefitinib. CONCLUSIONS: Our findings highlight the potential of the CRGs risk score as an independent and promising biomarker for clinical prognosis and immunotherapy sensitivity in HCC patients.


Asunto(s)
Carcinoma Hepatocelular , Neoplasias Hepáticas , Humanos , Carcinoma Hepatocelular/tratamiento farmacológico , Carcinoma Hepatocelular/genética , Neoplasias Hepáticas/tratamiento farmacológico , Neoplasias Hepáticas/genética , Pronóstico , Inmunoterapia , Sorafenib/uso terapéutico , Microambiente Tumoral , Apoptosis
13.
Org Lett ; 24(11): 2175-2180, 2022 Mar 25.
Artículo en Inglés | MEDLINE | ID: mdl-35285649

RESUMEN

Herein, we disclose a highly stereoselective Rh(II)-catalyzed 1,4-acyl rearrangement of selenium esters and α-diazo carbonyl compounds, which provides an efficient method for synthesizing tetrasubstituted vinyl selenides. Furthermore, this reaction also offers a synthetic tool for medium and large ring compounds.

14.
J Colloid Interface Sci ; 624: 219-232, 2022 Oct 15.
Artículo en Inglés | MEDLINE | ID: mdl-35660890

RESUMEN

Polymeric N-rich carbon nitride of C3N5 is being utilized as a new visible-light-driven catalyst due to its narrower bandgap (∼2.0 eV). Building step-scheme (S-scheme) heterojunction by coupling with other semiconductors especially those own oxygen vacancies (OVs) can further upgrade the photocatalytic performance of C3N5-based photocatalysts. Herein, a novel S-scheme heterojunction of OVs mediated Bi2MoO6/C3N5 was fabricated by in-situ growing Bi2MoO6 nanoparticles with OVs on C3N5 nanosheets. Benefiting from the efficient separation and transfer of high energetic charge carriers by S-scheme charge migration, enriched structural defects, as well as the close contact by the in-situ growth, the heterojunction exhibited superior visible-light photocatalytic performance toward the removal of tetracycline (TC) and Cr(VI) than C3N5, Bi2MoO6, and their mechanical mixture under visible light. The TC degradation routes and the bio-toxicity evolution of TC were explored. Moreover, the photocatalytic mechanism for TC decomposition and Cr(VI) reduction over Bi2MoO6/C3N5 with OVs were elucidated. This work presents a newfangled vision for designing promising C3N5-based S-scheme heterojunction photocatalysts for pollution control.


Asunto(s)
Bismuto , Oxígeno , Antibacterianos/química , Bismuto/química , Cromo , Molibdeno , Nitrilos , Oxígeno/química , Tetraciclina
15.
J Colloid Interface Sci ; 619: 307-321, 2022 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-35398762

RESUMEN

Constructing novel Z-scheme heterojunctions is an effective strategy for obtaining high-performance photocatalysts. Herein, tetra (4-carboxyphenyl) porphyrin (TCPP) and graphene quantum dots (GQDs) were loaded on the surface of Bi2MoO6 (BMO) to fabricate novel Z-scheme heterojunctions of TCPP/G/BMO. Especially, TCPP/G/BMO-2 showed an exceptional visible-light photoactivity for tetracycline (TC) degradation (81.0%, 40 min) and Cr(VI) reduction (90.7%, 60 min), respectively by 2.38 folds and 2.96 folds enhancement compared to sole Bi2MoO6. The substantial enhancement of activity is attributed to the synergy effect of the Z-scheme charge transfer mechanism and the improved light absorption. The degradation pathways of TC were inferred by determining the generated intermediates using high performance liquid chromatography-mass spectrometry (HPLC-MS), and the toxicity of the transformation products was assessed by Toxicity Estimation Software Tool (T.E.S.T). Overall, on the basis of trapping experiments and electron spin resonance spectra (ESR) analysis, the photocatalytic mechanisms of Cr(VI) reduction and TC degradation by the TCPP/G/BMO Z-scheme heterojunction was proposed. This work indicates TCPP/G/BMO could be a promising photocatalyst for wastewater treatment.


Asunto(s)
Grafito , Porfirinas , Puntos Cuánticos , Antibacterianos , Bismuto , Catálisis , Cromo , Molibdeno , Puntos Cuánticos/toxicidad , Tetraciclina/toxicidad
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