1.
Eur J Immunol
; 52(8): 1362-1365, 2022 08.
Artículo
en Inglés
| MEDLINE
| ID: mdl-35527391
RESUMEN
Robust methods for manipulation of human B cells, isolated from healthy donors and patients with B cell disorders, has the potential to significantly accelerate B cell research. Our work describes a step-by-step protocol to perform electroporation-based screening of gene function in B cells through the use of Cas9 ribonuclecomplexes and in vitro produced mRNA.