Acoustic Trapping: An Emerging Tool for Microfabrication Technology.

The high throughput deposition of microscale objects with precise spatial arrangement represents a key step in microfabrication technology. This can be done by creating physical boundaries to guide the deposition process or using printing technologies; in both approaches, these microscale objects cannot be further modified after they are formed. The utilization of dynamic acoustic fields offers a novel approach to facilitate real-time reconfigurable miniaturized systems in a contactless manner, which can potentially be used in physics, chemistry, biology, as well as materials science. Here, the physical interactions of microscale objects in an acoustic pressure field are discussed and how to fabricate different acoustic trapping devices and how to tune the spatial arrangement of the microscale objects are explained. Moreover, different approaches that can dynamically modulate microscale objects in acoustic fields are presented, and the potential applications of the microarrays in biomedical engineering, chemical/biochemical sensing, and materials science are highlighted alongside a discussion of future research challenges.

Moth wings are acoustic metamaterials.

Metamaterials assemble multiple subwavelength elements to create structures with extraordinary physical properties (1-4). Optical metamaterials are rare in nature and no natural acoustic metamaterials are known. Here, we reveal that the intricate scale layer on moth wings forms a metamaterial ultrasound absorber (peak absorption = 72% of sound intensity at 78 kHz) that is 111 times thinner than the longest absorbed wavelength. Individual scales act as resonant (5) unit cells that are linked via a shared wing membrane to form this metamaterial, and collectively they generate hard-to-attain broadband deep-subwavelength absorption. Their collective absorption exceeds the sum of their individual contributions. This sound absorber provides moth wings with acoustic camouflage (6) against echolocating bats. It combines broadband absorption of all frequencies used by bats with light and ultrathin structures that meet aerodynamic constraints on wing weight and thickness. The morphological implementation seen in this evolved acoustic metamaterial reveals enticing ways to design high-performance noise mitigation devices.

Envelope correction for shear-longitudinal collinear wave mixing to extract absolute nonlinear acoustic parameters.

This paper addresses the effect of the excitation envelope on the generated nonlinear resonant signal (NRS) for collinear wave mixing of shear and longitudinal waves. The aim is to explore how the absolute material nonlinearity can be extracted accurately for any enveloped sinusoidal excitation signal. A finite difference time domain (FDTD) model was built to simulate the effect of input waveforms on the NRS. A change in the measured nonlinearity was seen as the input waveforms were changed from rectangular to Hanning windowed tone burst. The required waveform correction was derived theoretically and validated against the FDTD simulation. Experimental measurements were carried out for different waveforms at several input amplitudes, demonstrating its influence over the NRS. The theoretically derived correction factor, which is required to map the small NRS to the rectangular tone burst resonant amplitude, was validated experimentally. The correction was then used to extract one the fundamental Murnaghan constant (m). Comparatively, Hanning tone burst inputs showed lower variance in the extracted material property due to better control of the frequency bandwidth, relative to that of the transducers. This opens the opportunity to using Hanning windowed tone burst inputs reliably for the measurement of the absolute nonlinearity parameter and m through collinear shear-longitudinal wave mixing.

Holographic acoustic tweezers.

Acoustic tweezers use sound radiation forces to manipulate matter without contact. They provide unique characteristics compared with the more established optical tweezers, such as higher trapping forces per unit input power and the ability to manipulate objects from the micrometer to the centimeter scale. They also enable the trapping of a wide range of sample materials in various media. A dramatic advancement in optical tweezers was the development of holographic optical tweezers (HOT) which enabled the independent manipulation of multiple particles leading to applications such as the assembly of 3D microstructures and the probing of soft matter. Now, 20 years after the development of HOT, we present the realization of holographic acoustic tweezers (HAT). We experimentally demonstrate a 40-kHz airborne HAT system implemented using two 256-emitter phased arrays and manipulate individually up to 25 millimetric particles simultaneously. We show that the maximum trapping forces are achieved once the emitting array satisfies Nyquist sampling and an emission phase discretization below π/8 radians. When considered on the scale of a wavelength, HAT provides similar manipulation capabilities as HOT while retaining its unique characteristics. The examples shown here suggest the future use of HAT for novel forms of displays in which the objects are made of physical levitating voxels, assembly processes in the micrometer and millimetric scale, as well as positioning and orientation of multiple objects which could lead to biomedical applications.

Biomechanics of a moth scale at ultrasonic frequencies.

The wings of moths and butterflies are densely covered in scales that exhibit intricate shapes and sculptured nanostructures. While certain butterfly scales create nanoscale photonic effects, moth scales show different nanostructures suggesting different functionality. Here we investigate moth-scale vibrodynamics to understand their role in creating acoustic camouflage against bat echolocation, where scales on wings provide ultrasound absorber functionality. For this, individual scales can be considered as building blocks with adapted biomechanical properties at ultrasonic frequencies. The 3D nanostructure of a full Bunaea alcinoe moth forewing scale was characterized using confocal microscopy. Structurally, this scale is double layered and endowed with different perforation rates on the upper and lower laminae, which are interconnected by trabeculae pillars. From these observations a parameterized model of the scale's nanostructure was formed and its effective elastic stiffness matrix extracted. Macroscale numerical modeling of scale vibrodynamics showed close qualitative and quantitative agreement with scanning laser Doppler vibrometry measurement of this scale's oscillations, suggesting that the governing biomechanics have been captured accurately. Importantly, this scale of B. alcinoe exhibits its first three resonances in the typical echolocation frequency range of bats, suggesting it has evolved as a resonant absorber. Damping coefficients of the moth-scale resonator and ultrasonic absorption of a scaled wing were estimated using numerical modeling. The calculated absorption coefficient of 0.50 agrees with the published maximum acoustic effect of wing scaling. Understanding scale vibroacoustic behavior helps create macroscopic structures with the capacity for broadband acoustic camouflage.

Chemical Information Exchange in Organized Protocells and Natural Cell Assemblies with Controllable Spatial Positions.

An ultrasound-based platform is established to prepare homogenous arrays of giant unilamellar vesicles (GUVs) or red blood cell (RBCs), or hybrid assemblies of GUV/RBCs. Due to different responses to the modulation of the acoustic standing wave pressure field between the GUVs and RBCs, various types of protocell/natural cell hybrid assemblies are prepared with the ability to undergo reversible dynamic reconfigurations from vertical to horizontal alignments, or from 1D to 2D arrangements. A two-step enzymatic cascade reaction between transmitter glucose oxidase-containing GUVs and peroxidase-active receiver RBCs is used to implement chemical signal transduction in the different hybrid micro-arrays. Taken together, the obtained results suggest that the ultrasound-based micro-array technology can be used as an alternative platform to explore chemical communication pathways between protocells and natural cells, providing new opportunities for bottom-up synthetic biology.

Comparison of Time Domain and Frequency-Wavenumber Domain Ultrasonic Array Imaging Algorithms for Non-Destructive Evaluation.

Ultrasonic array imaging algorithms have been widely developed and used for non-destructive evaluation (NDE) in the last two decades. In this paper two widely used time domain algorithms are compared with two emerging frequency domain algorithms in terms of imaging performance and computational speed. The time domain algorithms explored here are the total focusing method (TFM) and plane wave imaging (PWI) and the frequency domain algorithms are the wavenumber algorithm and Lu's frequency-wavenumber domain implementation of PWI. In order to make a fair comparison, each algorithm was first investigated to choose imaging parameters leading to overall good imaging resolution and signal-to-noise-ratio. To reflect the diversity of samples encountered in NDE, the comparison is made using both a low noise material (aluminium) and a high noise material (copper). It is shown that whilst wavenumber and frequency domain PWI imaging algorithms can lead to fast imaging, they require careful selection of imaging parameters.

Fabrication of Micropatterned Dipeptide Hydrogels by Acoustic Trapping of Stimulus-Responsive Coacervate Droplets.

Acoustic standing waves offer an excellent opportunity to trap and spatially manipulate colloidal objects. This noncontact technique is used for the in situ formation and patterning in aqueous solution of 1D or 2D arrays of pH-responsive coacervate microdroplets comprising poly(diallyldimethylammonium) chloride and the dipeptide N-fluorenyl-9-methoxy-carbonyl-D-alanine-D-alanine. Decreasing the pH of the preformed droplet arrays results in dipeptide nanofilament self-assembly and subsequent formation of a micropatterned supramolecular hydrogel that can be removed as a self-supporting monolith. Guest molecules such as molecular dyes, proteins, and oligonucleotides are sequestered specifically within the coacervate droplets during acoustic processing to produce micropatterned hydrogels containing spatially organized functional components. Using this strategy, the site-specific isolation of multiple enzymes to drive a catalytic cascade within the micropatterned hydrogel films is exploited.

Acoustic Virtual Vortices with Tunable Orbital Angular Momentum for Trapping of Mie Particles.

Acoustic vortices can transfer angular momentum and trap particles. Here, we show that particles trapped in airborne acoustic vortices orbit at high speeds, leading to dynamic instability and ejection. We demonstrate stable trapping inside acoustic vortices by generating sequences of short-pulsed vortices of equal helicity but opposite chirality. This produces a "virtual vortex" with an orbital angular momentum that can be tuned independently of the trapping force. We use this method to adjust the rotational speed of particles inside a vortex beam and, for the first time, create three-dimensional acoustics traps for particles of wavelength order (i.e., Mie particles).

Acoustic radiation force exerted on a small spheroidal rigid particle by a beam of arbitrary wavefront: Examples of traveling and standing plane waves.

The analytical solution of the acoustic radiation force exerted by a beam of arbitrary shape on a small spheroidal rigid particle suspended in an ideal fluid is presented. The particle is assumed to be much smaller than the wavelength, i.e., the so-called long-wavelength approximation. Based on this theoretical development, closed-form expressions for the radiation force of a traveling and standing plane wave exerted on a prolate spheroidal particle are derived in the dipole approximation. As validation, the previous analytical result considering a standing wave interacting with a spheroid in axisymmetric configuration is recovered, as well as numerical results obtained with the boundary-element method.

Ultrasonic defect characterisation-Use of amplitude, phase, and frequency information.

This paper studies ultrasonic defect characterisation with the aim of reducing the characterisation uncertainty. Ultrasonic array data contain a mixture of responses from all reflecting features, and the scattering matrix for each defect can be extracted in post-processing, which describes how ultrasonic waves at a given incident angle are scattered by a defect. In this paper, it is shown that defect characterisation performance can be improved by the inclusion of phase and frequency information relative to current single-frequency-amplitude approaches. This superior characterisation performance is due to the increased number of informative principal components (PCs) and higher signal-to-noise ratios in the PC directions. Scattering matrix phase measurement is very sensitive to localisation errors, and an effective approach is proposed, which can be used to reliably extract phase from experimental data. Nine elliptical defects having different aspect ratios and orientation angles are characterised experimentally. The complex multi-frequency defect database has achieved up to 90.60% reduction in the quantified sizing uncertainty compared to the results obtained using only the amplitude at a single frequency.

Acoustically trapped colloidal crystals that are reconfigurable in real time.

Photonic and phononic crystals are metamaterials with repeating unit cells that result in internal resonances leading to a range of wave guiding and filtering properties and are opening up new applications such as hyperlenses and superabsorbers. Here we show the first, to our knowledge, 3D colloidal phononic crystal that is reconfigurable in real time and demonstrate its ability to rapidly alter its frequency filtering characteristics. Our reconfigurable material is assembled from microspheres in aqueous solution, trapped with acoustic radiation forces. The acoustic radiation force is governed by an energy landscape, determined by an applied high-amplitude acoustic standing wave field, in which particles move swiftly to energy minima. This creates a colloidal crystal of several milliliters in volume with spheres arranged in an orthorhombic lattice in which the acoustic wavelength is used to control the lattice spacing. Transmission acoustic spectroscopy shows that the new colloidal crystal behaves as a phononic metamaterial and exhibits clear band-pass and band-stop frequencies which are adjusted in real time.

Observation of orbital angular momentum transfer from bessel-shaped acoustic vortices to diphasic liquid-microparticle mixtures.

We observe distinct regimes of orbital angular momentum (OAM) transfer from two-dimensional Bessel-shaped acoustic vortices to matter. In a homogeneous diphasic mixture of microparticles and water, slow swirling about the vortex axis is seen. This effect is driven by the absorption of OAM across the mixture, the motion following the OAM density distribution. Larger particles are formed into clusters by the acoustic radiation force, making the mixture nonhomogeneous. Here, the OAM transfer to the microparticle clusters dominates and they spin at high speeds entraining the surrounding fluid.

Air-Coupled Ultrasonic Arrays for Assessment of Pipe Internal Geometry and Surface Condition.

This article explores what useful information can be retrieved from pipeline interiors using an air-coupled ultrasonic array. Experiments are performed using an array, custom array controller, and supporting electronics controlled by a Raspberry Pi 4, mounted on board a crawler robot. A 64-transducer 40-kHz array configuration is selected based on uniformity of imaging amplitude over the circumference of the pipe wall. Testing revealed joints between pipe sections could be imaged at high amplitude, and that angular displacement between sections produced a different response to a properly aligned joint, potentially enabling detection of faulty joints. The surface roughness of some pipes also provides enough backscatter to be imaged, which is useful for detecting regions of corrosion. It was also found that reflections from the pipe wall in the plane of the array allow imaging of the wall shape. This can indicate the presence of junctions, as well as detect ovality to within 1%. These in-plane wall reflections were also found to be a source of low-amplitude coherent noise throughout the imaging domain, which is of similar amplitude to small (< 10 mm) through-holes in the pipe wall.

Ultrasound modulates neuronal potassium currents via ionotropic glutamate receptors.

BACKGROUND: Focused ultrasound stimulation (FUS) has the potential to provide non-invasive neuromodulation of deep brain regions with unparalleled spatial precision. However, the cellular and molecular consequences of ultrasound stimulation on neurons remains poorly understood. We previously reported that ultrasound stimulation induces increases in neuronal excitability that persist for hours following stimulation in vitro. In the present study we sought to further elucidate the molecular mechanisms by which ultrasound regulates neuronal excitability and synaptic function. OBJECTIVES: To determine the effect of ultrasound stimulation on voltage-gated ion channel function and synaptic plasticity. METHODS: Primary rat cortical neurons were exposed to a 40 s, 200 kHz pulsed ultrasound stimulus or sham-stimulus. Whole-cell patch clamp electrophysiology, quantitative proteomics and high-resolution confocal microscopy were employed to determine the effects of ultrasound stimulation on molecular regulators of neuronal excitability and synaptic function. RESULTS: We find that ultrasound exposure elicits sustained but reversible increases in whole-cell potassium currents. In addition, we find that ultrasound exposure activates synaptic signalling cascades that result in marked increases in excitatory synaptic transmission. Finally, we demonstrate the requirement of ionotropic glutamate receptor (AMPAR/NMDAR) activation for ultrasound-induced modulation of neuronal potassium currents. CONCLUSION: These results suggest specific patterns of pulsed ultrasound can induce contemporaneous enhancement of both neuronal excitability and synaptic function, with implications for the application of FUS in experimental and therapeutic settings. Further study is now required to deduce the precise molecular mechanisms through which these changes occur.

Coherent acoustic wave propagation in media with pair-correlated spheres.

Propagation of plane compressional waves in a non-viscous fluid with a dense distribution of identical spherical scatterers is investigated. The analysis is based on the multiple scattering approach proposed by Fikioris and Waterman, and is generalized to include arbitrary choice of the pair-correlation functions used to represent the distribution of the scatterers. A closed form solution for the effective wavenumber as a function of the concentration of pair-correlated finite-size spheres is derived up to the second order. In the limit of uncorrelated point-scatterers, this solution is identical to that obtained by Lloyd and Berry. Different pair-correlation functions are exemplified and compared, and the resulting differences discussed.

Dynamic patterning of microparticles with acoustic impulse control.

This paper describes the use of impulse control of an acoustic field to create complex and precise particle patterns and then dynamically manipulate them. We first demonstrate that the motion of a particle in an acoustic field depends on the applied impulse and three distinct regimes can be identified. The high impulse regime is the well established mode where particles travel to the force minima of an applied continuous acoustic field. In contrast acoustic field switching in the low impulse regime results in a force field experienced by the particle equal to the time weighted average of the constituent force fields. We demonstrate via simulation and experiment that operating in the low impulse regime facilitates an intuitive and modular route to forming complex patterns of particles. The intermediate impulse regime is shown to enable more localised manipulation of particles. In addition to patterning, we demonstrate a set of impulse control tools to clear away undesired particles to further increase the contrast of the pattern against background. We combine these tools to create high contrast patterns as well as moving and re-configuring them. These techniques have applications in areas such as tissue engineering where they will enable complex, high fidelity cell patterns.

Strategies for guided acoustic wave inspection using mobile robots.

Continuous non-destructive monitoring of large-scale structures is extremely challenging with traditional manual inspections. In this paper, we explore possible strategies that a collection of inspection robots could adopt to address this challenge. We envision the continuous inspection of a plate performed by multiple robots or a single robot that combines measurements from multiple locations. The robots use guided ultrasonic waves to interrogate a localized region for defects such as cracking or corrosion. In the detection stage, the receiver operating characteristic defines a detection zone in which a defect is thought to be present. In the localization stage, further measurements are made to locate the defect within this zone to a certain accuracy. We then address the question of what additional measurements are needed to achieve a given level of performance in the presence of uncertainty in robot locations? We explore this problem with Monte Carlo simulations that reveal the compromise between number of robots and performance in terms of defect location accuracy. In an experimental validation example on an aluminium plate, we show that six measurements arranged in a pentagon with a central measurement point leads to localization errors of similar magnitude to the uncertainty in sensor location.

Ultrasonic Nondestructive Characterization of Blockages and Defects in Underground Pipes.

This article explores the use of a 40-kHz air-coupled ultrasonic array in detecting and imaging blockages and defects in buried pipes with 17-26 wavelengths in diameter at short ranges (approximately 20-60 wavelengths). In particular, the imaging performance of arrays with different numbers of transducers is quantified and compared to establish how many are required for adequate performance. Even low numbers of transducers (<25) are capable of producing -6-dB contours of blockages that match reference images to within 95% by restricting the aperture to maintain element density. However, doing so also limits the resolving power, so arrays with more transducers ultimately image better by having great enough density even at larger apertures. Using <25 transducers also gives a poor contrast ratio of features above background noise (as low as 2), resulting in low tolerance for detection and producing unusable images in some cases. More robust performance is achieved with larger numbers of transducers, which achieves sufficient contrast. All images of planar objects feature a low-amplitude band due to interference between direct reflections and reflections via the pipe wall, which was verified by comparison to simulation. When tested in larger pipes in a deployment case, the low-amplitude band was notably larger but was found to decrease in size at longer ranges.

Moth wings as sound absorber metasurface.

In noise control applications, a perfect metasurface absorber would have the desirable traits of not only mitigating unwanted sound, but also being much thinner than the wavelengths of interest. Such deep-subwavelength performance is difficult to achieve technologically, yet moth wings, as natural metamaterials, offer functionality as efficient sound absorbers through the action of the numerous resonant scales that decorate their wing membrane. Here, we quantify the potential for moth wings to act as a sound-absorbing metasurface coating for acoustically reflective substrates. Moth wings were found to be efficient sound absorbers, reducing reflection from an acoustically hard surface by up to 87% at the lowest frequency tested (20 kHz), despite a thickness to wavelength ratio of up to 1/50. Remarkably, after the removal of the scales from the dorsal surface the wing's orientation on the surface changed its absorptive performance: absorption remains high when the bald wing membrane faces the sound but breaks down almost completely in the reverse orientation. Numerical simulations confirm the strong influence of the air gap below the wing membrane but only when it is adorned with scales. The finding that moth wings act as deep-subwavelength sound-absorbing metasurfaces opens the door to bioinspired, high-performance sound mitigation solutions.