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The papers were searched in databases including PubMed, Scopus, Web of science, Embase, Science Direct until 21 September 2023 and meta-analysis of concentration of PTEs in honey was performed using random effects model based on country subgroups. The non-carcinogenic risk and carcinogenic risk were calculated by Target Hazard Quotient (THQ) and Cancer Risk (CR), respectively. The sort of PTEs in honey based on pooled concentration was Cu (1.10E + 02 µg/kg) > Ni (1.80E + 01 µg/kg) > Pb (2.60E + 00 µg/kg) > Hg (1.50E + 00 µg/kg) > Cd (5.10E-01 µg/kg) > As (4.80E-01 µg/kg). The concentration of PTEs in the countries of Ethiopia (As), Jordan (Cd and Pb), Thailand (Ni), Brazil (Cu and MeHg) was observed to be higher than other countries. The non-carcinogenic risk was less than 1, hence, the consumption of honey does not have non-carcinogenic risk. Except children in Nigeria, CR due to iAs in honey was acceptable for the both adults and children in the other countries, Therefore, the consumption of honey does not have carcinogenic risk.
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Emerging noncommunicable diseases (NCDs) with premature mortality are one of the major concerns in health supervising organizations of the world. High intake of energy, salt, sugar, fat, and trans fatty acids are introduced as dietary risk factors of NCDs. The status of food risk factors is presented in Traffic Light (TL) food labeling through colors of red, yellow, and green as the signs of stop, wait and watch and go, respectively. This stepwise interventional study examined whether TL education can result in choosing healthier food via assessing the impact of face-to-face educational sessions on knowledge, attitudes, and practices of 673 respondents toward TL food labeling through a self-administered and structured questionnaire. Paired t test or alternatively Wilcoxon test was used to evaluate the influence of education in total scores of knowledge, attitudes, and practices in test-retest. Results indicated that before education, the average of the scores for the knowledge, attitudes, and practices was 1.003 ± 0.69, 10.97 ± 1.86, and 1.60 ± 1.84, respectively. After education, the scores were increased to 8.72 ± 3.11, 15.95 ± 2.64, and 8.42 ± 1.69. Significant differences in the scores of the respondents were observed before and after education (p < .05). This study revealed that the intervention of education had positive effect on the knowledge, attitudes, and practices of the studied population. Even though general awareness about the food risk factors of NCDs existed, special education is required to make public TL and food labeling information usage as an actual guidance for every food item in Iran.
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Etiquetado de Alimentos , Conocimientos, Actitudes y Práctica en Salud , Alimentos , Humanos , Irán , Encuestas y CuestionariosRESUMEN
BACKGROUND: Ulcerative colitis (UC) is an inflammatory bowel disease (IBD) that is widely identified worldwide. This study aimed to investigate the phenotypic characterization and molecular typing of Clostridium difficile isolates among patients with UC at an inflammatory bowel disease clinic in Iran. METHODS: In this cross-sectional study, conducted from April 2015 to December 2015, 85 UC patients were assessed for C.difficile infection (CDI). C. difficile isolates were characterized based on their toxin profile and antimicrobial resistance pattern. Multi-locus sequence typing analysis (MLST) and PCR ribotyping were performed to define the genetic relationships between different lineages of toxigenic strains. RESULTS: The prevalence of C. difficile isolates was 31.8% (27/85) in patients, of those 15 patients (17.6%) had CDI. Three different sequence types (STs) identified based on MLST among the toxigenic isolates, that is ST54 (33.3%), ST2 (53.3%), and ST37 (13.6%). C. difficile strains were divided into four different PCR-ribotypes (012, 014, 017 and IR1). The most common ribotype was 014 accounting for 48.3% (7/15) of all strains. The strains isolated during the first episode and recurrence of CDI usually belonged to PCR ribotype 014 (ST2). A high rate of CDI recurrence (14.1%, 12/85) experienced in UC patients. Colonization of the gastrointestinal tract with non-toxigenic C. difficile strains was frequent among patients with mild disease. All C. difficile isolates were susceptible to metronidazole, and vancomycin, 86 and 67% of isolates were resistant to clindamycin and erythromycin respectively. There was no correlation between the toxin type and antibiotic resistance (p > 0.05). CONCLUSION: Overall CDI is rather prevalent in UC patients. All patients with CDI experienced moderate to severe disease and exposed to different antimicrobial and anti-inflammatory agents. Close monitoring and appropriate management including early detection and fast treatment of CDI will improve UC outcomes.
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Infecciones por Clostridium/diagnóstico , Colitis Ulcerosa/diagnóstico , Heces/microbiología , Adulto , Antibacterianos/farmacología , Antibacterianos/uso terapéutico , Antiinflamatorios/uso terapéutico , Clostridioides difficile/efectos de los fármacos , Clostridioides difficile/genética , Clostridioides difficile/aislamiento & purificación , Infecciones por Clostridium/tratamiento farmacológico , Infecciones por Clostridium/microbiología , Colitis Ulcerosa/tratamiento farmacológico , Colitis Ulcerosa/microbiología , Estudios Transversales , ADN Bacteriano/genética , ADN Bacteriano/metabolismo , Farmacorresistencia Bacteriana , Femenino , Tracto Gastrointestinal/microbiología , Humanos , Modelos Logísticos , Masculino , Pruebas de Sensibilidad Microbiana , Persona de Mediana Edad , Tipificación de Secuencias Multilocus , Recurrencia , RibotipificaciónRESUMEN
In this study, a new separation technique based on membrane extraction is described for the determination of melamine in dry milk. The water-compatible cellulose acetate membrane, which is photografted by melamine imprinted nanospheres, was prepared by placing the membrane into the polymerization solution containing methacrylic acid as a functional monomer, ethylene glycol dimethacrylate as cross-linker, acetonitrile as porogen, and melamine as the template molecule. The characterization of the polymeric membrane was performed by Fourier transmission infrared spectroscopy and scanning electron microscopy. This integrated composite membrane was used as a solid-phase extraction medium for the extraction of melamine from dry milk samples. Various parameters affecting the extraction efficiency of the membrane were evaluated. The results showed higher binding capacity for melamine imprinted membranes in comparison with the nonimprinted membranes. High-performance liquid chromatography analysis showed that the extraction of melamine from dry milk by the photografted cellulose acetate membrane had a linear calibration curve in the range of 0.02-11.80 µg/mL with an excellent precision of 2.73%. The limit of detection and quantification of melamine was 0.007 and 0.020 µg/mL, respectively. The recoveries of melamine were in the range of 88.7-94.8%.
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Celulosa/análogos & derivados , Leche/química , Impresión Molecular , Triazinas/aislamiento & purificación , Animales , Cromatografía Líquida de Alta Presión , Nanosferas , Polímeros , Extracción en Fase SólidaRESUMEN
To determine the prevalence of Clostridium difficile in farm animals from slaughterhouse through to retail stage, a total of 750 samples of feces, posteviscerated and washed carcass were collected from cattle, camels, goats, and sheep in Isfahan, Iran. The overall prevalence of C. difficile in feces, posteviscerated and washed carcass were 20 (13.3%), 23 (15.3%), and 11 (7.3%), respectively; while C. difficile was isolated from 79 (26.3%) retail samples. Twenty-nine (3.8%) isolates were toxigenic, with most toxigenic isolates (n = 17, 5.6%) identified from the retail stage. All toxigenic isolates harbored tcdA and tcdB; however, all were negative for cdtB. The 29 isolates were classified into 21 different ribotypes. This study revealed evidence of existence of toxigenic C. difficile in farm animal feces and meat in Iran.
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Clostridioides difficile/aislamiento & purificación , Heces/microbiología , Contaminación de Alimentos/análisis , Microbiología de Alimentos , Carne/microbiología , ADP Ribosa Transferasas , Mataderos , Animales , Proteínas Bacterianas , Toxinas Bacterianas , Camelus , Bovinos , Clostridioides difficile/clasificación , Clostridioides difficile/genética , Enterotoxinas , Cabras , Irán , OvinosRESUMEN
BACKGROUND: The recent increment of the incidence of Community Associated Clostridium difficile Infection (CA)-CDI has led to speculation that this disease is associated to foodborne transmission. Therefore it is critical to establish the community sources of CDI in order to implement the appropriate interventions. The present study was conducted to evaluate the prevalence of C. difficile in seasoned hamburger and examine the sources of C. difficile dispersal in hamburger processing plants. A total of 211 samples including hamburger ingredients, the final product, processing equipment and food contact surfaces were collected from seven hamburger processing plants to evaluate the routes of dispersal of C. difficile. The samples were assessed for the occurrence of C. difficile using culture and polymerase chain reaction (PCR) methods. All isolates were screened for the existence of toxin A, B and binary toxin genes. In addition, isolates were subjected to PCR ribotyping. RESULTS: Overall, 9/211 (4.2%) samples were positive. Toxigenic C. difficile were detected from 2/7 (28.5%) hamburger processing plants, in (3/54) 5.6% of beef meat samples, (2/56) 3.5% of swabs taken from the environment and (4/56) 7.1% of hamburger samples after both molding and freezing. C. difficile was not found in 45 non-meat ingredients including 14 defrosted onions, 14 textured soy proteins and 17 seasonings. All isolates contained tcdB gene while 7 strains were positive for tcdA and two remaining strains were negative for tcdA. None of the isolates harbored binary toxin gene (cdtB). PCR ribotyping of 9 isolates categorized into four ribotypes (IR21, IR 22, IR 23 and IR24). Ribotype IR 22 was the most common type 6/9 (66.6%) found. This genotype was isolated from raw meat, environmental samples and hamburger after both forming and freezing in one processing plant, suggesting raw beef meat as a possible major source of contamination. CONCLUSIONS: Hyper-virulent strains of ribotype were not found in this study however, occurrence of other toxicgenic strains indicate the public health significance of contamination of this product.
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Clostridioides difficile/aislamiento & purificación , Microbiología Ambiental , Microbiología de Alimentos , Toxinas Bacterianas/genética , Clostridioides difficile/clasificación , Clostridioides difficile/genética , Industria de Procesamiento de Alimentos , Variación Genética , Irán , Prevalencia , RibotipificaciónRESUMEN
In this study, an attempt was made to meta-analyzed the concentration of potentially toxic elements (PTEs) in shrimps tissue of Mediterranean Sea and health risk of consumers was estimated. Search was conducted in international databases includes Scopus, PubMed, Embase, Science Direct and Web of Science from 1 January 2010 to 20 July 2023. The random effects model used to meta-analysis of concentration of PTEs in shrimp in subgroups. In addition, non-carcinogenic and carcinogenic risks for adults and children were calculated using target hazard quotient (THQ) and cancer risk (CR). Meta-analysis concentration of PTEs in shrimps was conducted using random effects model based on country subgroups. The rank order of PTEs based on mean (pooled) level in fillet of shrimps was Fe (15.395 mg/kg-ww) > Zn (10.428 mg/kg-ww) > Cu (6.941 mg/kg-ww) Pb (5.7 mg/kg-ww) > Ni (1.115 mg/kg-ww) > As (0.681 mg/kg-ww) > Cd (0.412 mg/kg-ww) > Hg (0.300 mg/kg-ww). THQ level in adults and children due to Cd and Pb in Italy was higher than 1 value. THQ level in adults and children due to Cu, Ni, Fe, Zn and inorganic As was lower than 1 value. CR due to inorganic As in Greece and Türkiye for adults and children was higher than 1E-6 value. Therefore, it was recommended to continuously monitor and reduce the concentration of PTEs in shrimps in Italy, Greece and Türkiye, especially.
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Common carp (Cyprinus carpio) is one of the most consumed fish in the world and can be exposed to various forms of pollution, such as potential toxic elements (PTEs). Several studies have been conducted on the concentration of PTEs in common carp fish. The aims of the current study were to meta-analyze the concentration of PTEs in common carp fish and estimate human health risks in consumers. A search was conducted in international databases, including Scopus, PubMed, Science Direct, Web of Science, and Embase to retrieve papers up to January 20, 2024. The non-carcinogenic risk due to PTEs in fish fillets was calculated via the target hazard quotient (THQ), and the carcinogenic risk due to iAs in fish fillets was calculated via cancer risk (CR). The highest concentrations of Cu, methyl-Hg, and Ni were observed in the fillets of common carp fish. The non-carcinogenic risk was lower than 1 in all countries; hence, consuming common carp fish does not pose a non-carcinogenic risk. Adult consumers in Iraq were exposed to an unacceptable carcinogenic due to iAs in common carp fish. Hence, it is recommended that plans be conducted to reduce the concentration of PTEs in common carp fish in Iraq.
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Fortification of highly-consumed foods such as bread is an easy and cheap strategy to combat the iron deficiency anemia. However, there have sometimes been some side effects such as iron overload and digestive problems. Accordingly, this study aimed to examine the amount of iron as well as its non-carcinogenic risks in commonly-used types of Iranian flour and breads (Barbary, Lavash, and Tafton) in Isfahan, Iran. Iron concentration of 100 samples of flour and breads were measured by Inductively Coupled Plasma Optical Emission Spectrometer. Moreover, the non-carcinogenic health risk of iron in fortified breads was estimated by Target Hazard Quotient (THQ) in Monte Carlo Simulation technique. The limit of detection (LOD) and limit of quantification (LOQ), with a recovery level of 95%, were 1.8 × 10-5 and 5.9 × 10-5 mg/kg, respectively. The total mean concentration of iron in flour (53.48 ± 22.49 mg/kg) and bread (39.02 ± 22.63 mg/kg) samples was within the standard recommended range (40-85 mg/kg) in Iran. THQ for adults and children was equal to 0.53 and 2.48. respectively. Hence, non-carcinogenic risk of iron through bread consumption was acceptable for adults, while it was not acceptable for children as a sensitive group. Consequently, it is required to rescreen the flour and bread fortification program in Iran according to the comprehensive risk assessment studies.
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In recent years, food and packaging industries have worked together to minimize food wastes. Fruit and vegetable by-products, which are known to be among the most abundant food wastes and a great source of bioactive compounds, have the potential to improve food product packaging properties. The antioxidant and antimicrobial properties of pomegranate peel in food active packaging have been the subject of numerous studies. Pomegranate peel has an impact on the films' microstructure and physical properties, such as thickness, water vapor permeability, mechanical properties, optical properties, and thermal properties. Moreover, pomegranate peel incorporated films demonstrate great antioxidant and antimicrobial properties. Reviewing current advancements in the physical and functional properties of active packaging films containing pomegranate peel is the goal of this study.
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BACKGROUND: Regarding the importance of the prevention of non-communicable diseases (NCDs) and higher consumption of salt among the Iranian population than the level recommended by the World Health Organization, the aim of this study was to evaluate the accuracy of the salt mentioned in the traffic light labelling of nuts and seeds. Study Design: A cross-sectional study. METHODS: A total of 53 packaged nuts and seeds, including 7, 8, 9, 9, 10, and 10 samples of pumpkin, pistachios, almond, sunflower, peanut, and watermelon nuts and seeds, respectively, with traffic light labelling, were randomly purchased from several local markets in Isfahan, Iran. The amount of sodium was measured by the inductively coupled plasma-optical emission spectroscopy technique and then multiplied by 2.5 to achieve the amount of salt. RESULTS: Varying levels of traffic light labeling value accuracy were observed in most of the samples. In the almond, pistachio, peanut, and watermelon groups, the average amount of laboratory value had a statistically significant difference with the label value (P<0.05). CONCLUSION: The results demonstrated that the salt content of 82% of the studied samples had discrepancies with the values stated on the traffic light labelling. The presentation of an accurate amount of salt content is essential for promoting healthy eating habits and enabling individuals to make informed choices about their diet. It is recommended that regulatory authorities should review labelling guidelines and enforce stricter compliance to ensure accurate representation of salt content on packaged foods.
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Etiquetado de Alimentos , Nueces , Semillas , Irán , Nueces/química , Estudios Transversales , Semillas/química , Humanos , Cloruro de Sodio Dietético/análisis , Prunus dulcis/química , Citrullus/química , Pistacia/químicaRESUMEN
BACKGROUND: Non-small cell lung cancer (NSCLC) patients with COVID-19 have an excessive chance of morbidity and mortality. The fecal-nasopharyngeal microbiota compositions of NSCLC patients were assessed in this study. METHODS: In total, 234 samples were collected from 17 NSCLC patients infected with COVID-19, 20 NSCLC patients without confirmed COVID-19, 40 non NSCLC patients with COVID-19, and 40 healthy individuals. RESULTS: In lung microbiota, the abundance of Streptococcus spp. in NSCLC patients with confirmed COVID-19 was significantly higher than the two control groups. Pseudomonas aeruginosa and Staphylococcus aureus were listed as the most frequent pulmonary bacterial groups that colonized COVID-19 patients. In fecal specimens, the numbers of Bacteroidetes, Firmicutes, and Actinobacteria phyla were significantly higher amongst NSCLC patients with COVID-19. NSCLC patients infected with COVID-19 showed lower levels of Lactobacillus spp., Akkermansia muciniphila, and Bifidobacterium spp. The counts of Streptococcus spp., in NSCLC patients with COVID-19 were significantly higher than those of healthy individuals (8.49±0.70 log CFU/g wet feces vs 8.49±0.70 log CFU/g wet feces). Prevotella spp. were enriched in the gut and respiratory tracts of COVID-19 patient groups. The unbiased analysis showed an increment in Enterococcus spp., Streptococcus spp., and Prevotella spp. CONCLUSION: Eventually, it was found that compared to control groups, COVID-19 patients with NSCLC showed diminished gut bacteria diversity and increase in Lactobacillus spp., A. muciniphila, and Bifidobacterium spp. The overgrowth of Enterococcus spp., Streptococcus spp., and Prevotella spp. could be potential predictive biomarkers in the gut-lung axis of NSCLC patients with COVID-19.
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COVID-19 , Carcinoma de Pulmón de Células no Pequeñas , Coinfección , Neoplasias Pulmonares , Microbiota , Humanos , PulmónRESUMEN
Currently, food safety hazards have introduced as one of the most important threats to public health worldwide. Considering numerous crises in the field of food safety at global, regional, and national levels, and their impact on the physical and mental health of consumers, it is very vital to evaluate risk communication strategies in each country. Food safety risk communication (FSRC) aims to provide the means for individuals to protect their health from food safety risks and make informed decisions about food risks. The purpose of this study is to present FSRC as one of the key parts of risk analysis, its importance considering the prevalence of food contamination and recent crises related to food. Additionally, the stages of implementation of FSRC are mentioned. In FSRC, it is essential to comply with the principles and prerequisites. There are various strategies for FSRC nowadays. Different platforms for FSRC are rapidly evolving. Choosing and evaluating the appropriate strategy according to the target group, consensus of stakeholders, cooperation and coordination of risk assessors and risk managers have a significant impact in order to improve and implement FSRC.
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This survey was conducted to assess the metal(oids) content in 93 samples of bread, including barbari, lavash, and tafton, using inductive couple plasma/optical emission spectroscopy (ICP-OES) method and atomic absorption spectroscopy (AAS). The amounts of measured element were compared with the permissible limit set for bread by FAO/WHO and Iranian National Standardization Organization (INSO). The limit of detection (LOD) was ranged from 6.6â¯×â¯10-5 to 2.1â¯×â¯10-2 mgâ¯l-1 with recoveries ranged from 92% to 102%. The average concentrations of aluminum (Al), arsenic (As), boron (B), cadmium (Cd), iron (Fe), mercury (Hg), magnesium (Mg), sodium (Na), lead (Pb), and zinc (Zn) in bread were 29.88⯱â¯8, 0.03⯱â¯0.004, 12.77⯱â¯3.70, 0.01⯱â¯0.006, 34.16⯱â¯8.95, 0.01⯱â¯0.008, 346.07⯱â¯36.08, 3314.81⯱â¯317.19, 0.24⯱â¯0.11, and 19.65⯱â¯4.66â¯mg Kg-1, respectively. Amounts of As, Cd, Hg, Mg, Pb, and Zn were lower, and those of Al, Fe, and Na were higher than the permissible limits defined by FAO/WHO. The Latin Hyper Cube (LHC) sampling results revealed that children were exposed to higher non-carcinogenic risk and adults were more threatened by carcinogenic risk. It is recommended to control the entrance of metals in bread in the farm-to-fork chain in order to prevent probable future health challenges.
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[This corrects the article DOI: 10.1016/j.toxrep.2023.02.015.].
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Clostridioides (Clostridium) difficile (C. difficile) is one of the essential enteropathogens in humans and livestock and is a severe health threat, according to the Centre for Disease Control and Prevention. Also, antimicrobials are one of the most critical risk factors for C. difficile infection (CDI). The present study examined the infection, antibiotic resistance, and genetic diversity of the C. difficile strains in the meat and feces of some native birds (chicken, duck, quail, and partridge) in the Shahrekord region, Iran, from July 2018 to July 2019. Samples were grown on CDMN agar after an enrichment step. To determine the toxin profile, the tcdA, tcdB, tcdC, cdtA, and cdtB genes were detected via multiplex PCR. The antibiotic susceptibility of these isolates was examined using the disk diffusion method and followed based on MIC and epsilometric test. 300 meat samples of chicken, duck, partridge, and quail and 1100 samples of bird feces were collected from six traditional farms in Shahrekord, Iran. Thirty-five meat samples (11.6%) and 191 fecal samples (17.36%) contained C. difficile. Moreover, five toxigenic samples isolated had 5, 1, and 3 tcdA/B, tcdC, and cdtA/B genes. Out of the studied strains isolated from the 226 samples, two isolates belonging to ribotype RT027 and one isolated RT078 profile related to native chicken feces were observed from chicken sample. The antimicrobial susceptibility testing showed that all the strains are resistant to ampicillin, 28.57% are resistant to metronidazole, and 100% were susceptible to vancomycin. Based on the results, it can be concluded that the raw meat of birds might be a source of resistant C. difficile that poses a hygienic threat to the consumption of native bird meat. Nevertheless, further studies are essential to understand additional epidemiological features of C. difficile in bird meat.
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Toxinas Bacterianas , Clostridioides difficile , Infecciones por Clostridium , Humanos , Animales , Clostridioides difficile/genética , Toxinas Bacterianas/genética , Clostridioides , Irán , Farmacorresistencia Microbiana , Heces , Pollos , Carne , Variación Genética , Clostridium , Infecciones por Clostridium/epidemiología , Infecciones por Clostridium/veterinaria , Pruebas de Sensibilidad MicrobianaRESUMEN
The effectiveness of selenium nanoparticle (SeNP)-enriched Lactiplantibacillus plantarum and Lactobacillus acidophilus was studied against Shiga toxin-producing Escherichia coli O157:H7 infection on the intestinal fragments and kidney tissue of BALB/c mice. Gut microbiota-targeted bacteria and E. coli O157:H7 counts were obtained by qPCR and PCR. Histology of ileum, colon, and kidney tissues and Stx secretions were analyzed until one-week post-infection. Mice fed with SeNP Lpb. plantarum in the preinfection feeding groups have lower E. coli O157:H7 counts and lower intestinal damage than those in the infected group. The lowest mean fecal probiotic counts were in the L. acidophilus group (7.61 log 10). In pretreatment groups of SeNP L. acidophilus and L. acidophilus, the mean counts of bacteria decreased to 104 CFU/g by day 7. The lowest Stx copy number was demonstrated in SeNP Lpb. plantarum feeding groups' day 7 (P < 0.05). Feeding groups with SeNP Lpb. plantarum had significantly higher members of Lactobacilli in their fecal microbiota than the control group on day 7. It was clarified that Se-enriched Lpb. plantarum and L. acidophilus can be useful as a method of preventing STEC infections. The viability of STEC infection exposure to selenium-enriched Lactobacillus spp. was decreased more than for non-Se-enriched Lactobacillus spp.
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BACKGROUND: Today, in response to consumer demand and market trends, the development of new packaging with better performance such as intelligent packaging has become more important. This packaging system is able to perform intelligent functions to increase shelf life, increase safety and improve product quality. OBJECTIVES: Recently, various types of packaging systems are available for meat products, especially cooked, fresh and processed meats. But because meat products are very perishable, monitoring their quality and safety in the supply chain is very important. This systematic article briefly reviews some of the recent data about the application of intelligent packaging in meat products. METHODS: The search was conducted in Google Scholar, Science Direct, Elsevier, Springer, Scopus, and PubMed, from April 1996 to April 2021 using a different combination of the following keyword: intelligent packaging, and meat. RESULTS: The results showed that the intelligent packaging presents several benefits compared to traditional packaging (e.g., antimicrobial, antioxidant, and shelf life extension) at the industrial processing level. Thus, these systems have been applied to improve the shelf life and textural properties of meat and meat products. CONCLUSIONS: It is necessary to control the number of intelligent compounds that are included in the packaging as they clearly influence the quality and nutritional properties as well as the final cost of the food products.
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Productos de la Carne , Animales , Conservación de Alimentos/métodos , Embalaje de Alimentos/métodos , Carne , AntioxidantesRESUMEN
Melamine is added illegally to milk and dairy products to increase the amount of apparent protein. This organic nitrogen rich chemical compound has been of great challenge in food safety based on its adverse effect on health. Therefore, the extraction and determination of melamine from milk is necessary. Recently, ionic liquid (ILs) as solvent usage has been noticeable for low melting point, low toxicity, high thermal stability, and high extraction capabilities in a wide range of separation processes. ILs are introduced as organic-inorganic salts and green solvents in microextraction preparation. Therefore, in this study, three ionic liquids ([C6mim][NTF2], [C4mim][NTF2] and [C2mim][NTF2] ILs) were prepared and employed as an extraction solvent in dispersive liquid-liquid microextraction (DLLME) of melamine from milk samples followed by HPLC-UV. The selected ILs were designed using three types of alkyl-imidazolium (as the short organic cations) and bis (tri fluoro methyl sulfonyl) imide as anion and characterized by ATR-FTIR spectra, carbon, and hydrogen Nuclear Magnetic Resonance spectroscopy (H&C-NMR) and energy-dispersive X-ray spectroscopy (EDX). These techniques confirmed the formation of functional groups, the structure of hydrogen and carbon atoms, and various elements of ionic bond between imidazolium and bis (tri fluoro methyl sulfonyl) imide. In the next step, the effect of significant parameters, including type and volume of ILs, adsorption time, pH of the sample solution, and sample volume, were optimized. Under the optimal conditions, the limits of detection (LOD), limits of quantification (LOQ), and linearity range were obtained 63.64 µg kg-1, 210.03 µg kg-1, and 210.03-1000 µg kg-1, respectively, for as prepared [C6mim][NTF2] as the best ILs. Notably, the achieved LOQ was lower than the maximum residue level (MRL) for the melamine residue in dairy products. Eventually, the proposed method was applied to detect melamine in milk samples, and the relative recoveries were examined as 79.6-105.0 %.
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Líquidos Iónicos , Microextracción en Fase Líquida , Animales , Solventes/química , Líquidos Iónicos/química , Cromatografía Líquida de Alta Presión/métodos , Leche , Imidas/química , Microextracción en Fase Líquida/métodos , Límite de Detección , Carbono , HidrógenoRESUMEN
Electrospun fibers (EFs) have emerged as promising one-dimensional materials for a myriad of research/commercial applications due to their outstanding structural and physicochemical features. Polymers of either synthetic or natural precursors are applied to design EFs as carriers for bioactive compounds. For engineering food systems, it is crucial to exploit polymers characterized by non-toxicity, non-immunogenicity, biocompatibility, slow/controllable biodegradability, and structural integrity. The unique attributes of protein-based biomaterials endow a wide diversity of desirable features to EFs for meeting the requirements of advanced food/biomedical applications. In this review paper, after an overview on electrospinning, different protein materials (plant- and animal-based) as biodegradable/biocompatible building blocks for designing EFs will be highlighted. The potential application of protein-based EFs in loading bioactive compounds with the intention to inspire interests in both academia and industry will be summarized. This review concludes with a discussion of prevailing challenges in using protein EFs for the bioactive vehicle development.