Hierarchically Annular Mesoporous Carbon Derived from Phenolic Resin for Efficient Removal of Antibiotics in Wastewater.

Antibiotics have become a new type of environmental pollutant due to their extensive use. High-performance adsorbents are of paramount significance for a cost-effective and environmentally friendly strategy to remove antibiotics from water environments. Herein, we report a novel annular mesoporous carbon (MCN), prepared by phenolic resin and triblock copolymer F127, as a high-performance adsorbent to remove penicillin, streptomycin, and tetracycline hydrochloride from wastewater. The MCNs have high purity, rich annular mesoporosity, a high surface area (605.53 m2/g), and large pore volume (0.58 cm3/g), improving the adsorption capacity and facilitating the efficient removal of penicillin, streptomycin, and tetracycline hydrochloride from water. In the application of MCNs to treat these three kinds of residual antibiotics, the adsorption amounts of tetracycline hydrochloride were higher than penicillin and streptomycin, and the adsorption capacity was up to 880.6 mg/g. Moreover, high removal efficiency (99.6%) and excellent recyclability were achieved. The results demonstrate that MCN adsorbents have significant potential in the treatment of water contaminated with antibiotics.

A simple integrated microfluidic platform for the research of hydrogels containing gradients in cell density induced breast cancer electrochemotherapy.

Cell density is important for tumour metastasis, treatment and prognosis. Characterizing changes in cell density for electrochemotherapy (ECT) can reveal sub-populations in pathological states, and adjust treatment program. In this work, a simple and convenient microfluidic platform was developed to study the effect cell density on ECT by integrating the improved cell gradient generator, cell culture chamber and indium tin oxide interdigital electrodes. Agarose, as extracellular matrix (ECM), was used to 3D cell culture to imitate in vivo microenvironment. The precision and reproducibility of cell density gradient with agarose solution were achieved because the hydrophobic modification of microchannels surface resulted in reducing cell adhesion and residue. ECT cytotoxicity assay with difference in cell densities was studied. The results showed that tumour cell density is one of the most factors for ECT treatment and ECT cytotoxicity has a certain of cell density-depended. But only electroporation on low cell density level, ECM would be one of the most key factors for ECT cytotoxicity, which would provide a new idea for chip-based cell assay in clinical diagnosis and drug screening in ordinary laboratories.

Inkjet printing-assisted single-cell microarray on a hydrophobic surface chip for real-time monitoring of enzyme kinetics at single-cell level.

A convenient, facile, and mask-free approach assay was developed for single-cell study by using a combination of inkjet printing technology and polydimethylsiloxane (PDMS) microchip-assisted processing. The inkjet printing technology resulted in 91% of the single-cell occupancy by individually spraying MCF-7 cells on a hydrophobic substrate and enabled the control over the number of cells with precision by strictly optimizing the printing parameters. Further, the microchip containing a cell chamber and straight channels was attached to the glass slide to explore the real-time performance of the cells. To address the performance attributes, the enzyme kinetics and various parameters of the post-printed MCF-7 cells, such as the levels of cell viability, reactive oxygen species (ROS), cell apoptosis, and proliferation, are monitored in real-time. Interestingly, high activity and proliferation, low level of ROS, and cell apoptosis demonstrated that the developed method provided a new way to the study of single-cell in-depth. Finally, ATP-induced cell proliferation of different cell number were analyzed, and the results would provide another perspective for the diagnosis and medical treatment.

A Fluorescent Sensor-Assisted Paper-Based Competitive Lateral Flow Immunoassay for the Rapid and Sensitive Detection of Ampicillin in Hospital Wastewater.

In this study, a convenient assay method has been developed based on labeled functional nucleic acids (H-DNA) and a competitive fluorescent lateral flow immunoassay (CF-LFI) for ampicillin (AMP) detection. Herein, we designed the tunable AMP probes for AMP detection based on the AMP aptamer, and the secondary DNA fragment. The probes can generate tunable signals on the test line (T line) and control line (C line) according to the concentration of AMP. The accuracy of detection was improved by optimizing the tunable AMP probes. Under the optimal conditions, the linear concentration of AMP detection is ranged from 10 to 200 ng/L with a limit of quantitation (LOQ) value of 2.71 ng/L, and the recovery is higher than 80.5 %. Moreover, the developed method shows the potential application for AMP detection in the hospital wastewater.

Isolation and Purification of Condensed Tannin from the Leaves and Branches of Prunus cerasifera and Its Structure and Bioactivities.

Prunus cerasifera has a rich resource and a weak utilization rate and its biological functions have been investigated. We found that the contents of total phenol (TP) in leaves and branches of Prunus cerasifera were 117.8 ± 8.8 and 100.04 ± 0.9 mg/g, respectively; the contents of soluble condensed tannin (SCT) were 73.95 ± 0.9 and 78.65 ± 4.1 mg/g, respectively; the structure of SCT containing afzelechin/epiafzelechin, catechin/epicatechin, and atechin/epicatechin as the main units and the SCT from leaves and branches exhibited better anti-tyrosinase and antioxidant activities. This study could clarify Prunus cerasifera condensed tannin resource availability and lay a theoretical foundation for its development as a natural antioxidant and tyrosinase inhibitor.

Effects of isoimperatorin on proliferation and apoptosis of human gastric carcinoma cells.

Resistance to apoptosis is an characteristic of cancer cells that serves a critical function in tumor development and represents a target for antitumor therapy. Isoimperatorin (ISOIM), a coumarin compound, exhibits antitumor functions in multiple types of tumor cells. However, its antitumor effects and molecular mechanisms with respect to gastric cancer have not been elucidated. The present study assessed the anti-proliferative and apoptotic effects of ISOIM on human BGC-823 gastric cancer cells and elucidated its underlying molecular mechanisms. Cell proliferation was evaluated using MTT assays. Analysis of cell morphology was performed by hematoxylin and eosin, Hoechst 33258 and acridine orange/ethidium bromide staining. In addition, cell cycle and apoptosis was evaluated using flow cytometry analysis; expression of apoptosis-associated proteins was studied by western blotting. The results of the present study revealed that ISOIM significantly inhibited cell proliferation by arresting the cell cycle at the G2/M phase and induced apoptosis by increasing Bcl-2-associated X (Bax) expression with a concomitant decrease in Bcl-2 expression, resulting in a decreased Bcl-2/Bax ratio compared with the control. In addition, ISOIM treatment also resulted in cytochrome c translocating from the mitochondria to the cytosol. Furthermore, caspase-3 was significantly activated in response to treatment with ISOIM, suggesting that apoptosis in BGC-823 cells is induced in the mitochondrial pathway. Taken together, the results of the present study indicate that ISOIM may significantly induce apoptosis in BGC-823 cells and that the pro-apoptotic mechanisms of ISOIM could be associated with the mitochondrial pathway.