RESUMEN
The role of interlinked positively charged amino acids in the mechanism of inhibition of a monomeric trypsin-like proteinase has been investigated using high molecular mass L-lysine homopolymers ranging from 3.8 to 109 kDa. The data show that the degree of polymerization enhances the inhibitory efficiency which is maximal for homopolymers with more than eighteen interlinked lysine residues. The inhibition is cooperative and, under the maximal inhibition conditions, nine lysine residues of the polymer are involved in the electrostatic binding to the enzyme. A limited conformational change of the protein molecule accompanies the transition from a fully active to a fully inactivated enzyme.
Asunto(s)
Endopeptidasas/metabolismo , Lisina/farmacología , Medicago sativa/enzimología , Inhibidores de Proteasas/farmacología , Polímeros/farmacología , Conformación ProteicaRESUMEN
Adenosine deaminase (adenosine aminohydrolase, EC 3.5.4.4) from Bacillus cereus NCIB 8122 has been purified to electrophoretic homogeneity by ammonium sulfate precipitation, gel filtration through Sephadex G-100, DEAE-Sephadex A-50 chromatography and ion-exchange HPLC on DEAE-Polyol. The enzyme activity is stabilized (at temperatures from 0 degrees C to 40 degrees C) by 50 mM NH4+ or K+, while it is irreversibly lost in the absence of these or a few other monovalent cations. Glycerol (24% by volume) helps the cation in stabilizing the enzyme activity above 40 degrees C, but also exerts per se a noticeable protecting effect at room temperature. B. cereus adenosine deaminase displays the following properties: Mr on Sephadex G-200, 68,000; Mr in SDS-polyacrylamide gel electrophoresis, 53,700; optimal pH-stability (in the presence of 50 mM KCl) over the range 8-11 at 4 degrees C, and maximal catalytic activity at 30 degrees C between pH 7 and 10; Km for adenosine around 50 microM over the same pH range and Km for 2'-deoxyadenosine around 400 microM.
Asunto(s)
Adenosina Desaminasa/aislamiento & purificación , Bacillus cereus/enzimología , Nucleósido Desaminasas/aislamiento & purificación , Adenosina Desaminasa/metabolismo , Cationes Monovalentes , Estabilidad de Enzimas , Glicerol/farmacología , CinéticaRESUMEN
A novel protease has been identified, purified and partially characterised from complete medium grown Spirulina platensis, which could be responsible for the selective proteolysis of phycobiliproteins. It is an 80 kDa homodimeric enzyme; its N-terminal sequence is not related to any known protease sequence. It hydrolyses native phycocyanins in both crude extracts and reconstructed systems with purified Allo- or C-phycocyanin. It is inactive on several native proteins, including ribulose-1,5-bisphosphate carboxylase. The two phycocyanins are degraded at different velocities since C-phycocyanin is the better substrate, in agreement with the earlier observations on the progress of the phycobilisome disassembly. Specificity for synthetic substrates and inhibitors strongly suggests its assignment to the serine-protease family. The enzyme, however, is insensitive to the commercially available protein inhibitors of trypsin-like proteases.
Asunto(s)
Cianobacterias/enzimología , Endopeptidasas/metabolismo , Ficocianina/metabolismo , Medios de Cultivo/farmacología , Cianobacterias/efectos de los fármacos , Cianobacterias/crecimiento & desarrollo , Electroforesis en Gel de Poliacrilamida , Endopeptidasas/química , Endopeptidasas/aislamiento & purificación , Hidrólisis , Cinética , Peso Molecular , Nitrógeno/administración & dosificación , FicobilisomasAsunto(s)
Bacillus subtilis/enzimología , Monoéster Fosfórico Hidrolasas/metabolismo , Sulfato de Amonio , Bacillus subtilis/crecimiento & desarrollo , Cromatografía en Gel , Guanosina , Nucleótidos , Hidrolasas Diéster Fosfóricas/metabolismo , Espectrofotometría , Esporas Bacterianas/enzimología , Rayos UltravioletaAsunto(s)
Nucleótidos de Adenina/análisis , Nucleótidos de Guanina/análisis , Nucleótidos/análisis , Fosfatasa Alcalina , Aminohidrolasas , Animales , Bovinos , Fenómenos Químicos , Química , Densitometría , Glucosiltransferasas , Métodos , Nucleósidos , Páncreas/enzimología , Monoéster Fosfórico Hidrolasas , Ribonucleasas , Serpientes , Espectrofotometría , Bazo/enzimología , Factores de Tiempo , Rayos Ultravioleta , Ponzoñas , Xantina OxidasaAsunto(s)
Bacillus subtilis/enzimología , Nucleotidasas/metabolismo , Hidrolasas Diéster Fosfóricas/metabolismo , Esporas/enzimología , Nucleótidos de Adenina , Adenosina , Aminohidrolasas , Bacillus subtilis/crecimiento & desarrollo , Nucleótidos de Citosina , Nucleótidos de Guanina , Espectrofotometría Ultravioleta , Esporas Bacterianas/crecimiento & desarrolloAsunto(s)
Nucleotidasas/análisis , Adenosina Monofosfato , Aminohidrolasas , Animales , Citidina , Nucleótidos de Citosina , Nucleótidos de Guanina , Guanosina , Nucleótidos de Inosina , Cinética , Métodos , Nucleotidasas/antagonistas & inhibidores , Pentosiltransferasa , Serpientes , Espectrofotometría Ultravioleta , Ponzoñas , Xantina OxidasaRESUMEN
ATP levels were measured in the liver and brain of rats acutely intoxicated with ethanol. The pretreatment of the animals with pyridoxine-pyrrolidon carboxylate (Metadoxine) prevented the marked fall in ATP concentration caused by ethanol in both organs.
Asunto(s)
Adenosina Trifosfato/análisis , Intoxicación Alcohólica/metabolismo , Química Encefálica/efectos de los fármacos , Hígado/análisis , Piridoxina/farmacología , Pirrolidinonas/farmacología , Ácido Pirrolidona Carboxílico/farmacología , Animales , Combinación de Medicamentos , Humanos , Hígado/efectos de los fármacos , Masculino , Ratas , Factores de TiempoRESUMEN
A procedure for the coupling at pH 7.2 of p-carboxy benzene diazonium chloride with rabbit muscle aldolase supported on phosphocellulose is described and some of the spectroscopic, structural and catalytic features of the material obtained are reported. The tetrameric azoenzyme is homogeneous in disc gel electrophoresis even in the presence of 8 M urea. Twelve molecules of the reactant are bound to the protein. Eight azocysteins are identified by both spectroscopic studies and amino acid analysis. The presence of one azohistidine is suggested by the spectroscopic data along with the presence of other, as yet unknown, chromophores. The azoaldolase shows unchanged catalytic properties using both D-fructose 1,6-bisphosphate and D-fructose 1-phosphate as substrates, as compared with the native enzyme. The pH profile of the enzyme activity is broadened towards the alkaline region but no changes occur in the physiological range of pH.
Asunto(s)
Compuestos Azo , Fructosa-Bifosfato Aldolasa , Aminoácidos/análisis , Animales , Sitios de Unión , Cromatografía por Intercambio Iónico , Dicroismo Circular , Electroforesis Discontinua , Fructosa-Bifosfato Aldolasa/aislamiento & purificación , Fructosa-Bifosfato Aldolasa/metabolismo , Concentración de Iones de Hidrógeno , Cinética , Músculos/enzimología , Unión Proteica , Conformación Proteica , Conejos , Solubilidad , Espectrofotometría , Espectrofotometría UltravioletaRESUMEN
Tryptophan room temperature phosphorescence in solution was detected in glutamic dehydrogenase from bovine liver and Escherichia coli with lifetimes of 1.2 and 0.65 s, respectively. Although these enzymes possess three and five tryptophanyl residues per polypeptide chain, respectively, the temperature dependence of the phosphorescence quantum yield estimates that the room temperature emission is due, in either case, to a single residue. Long triplet-state lifetimes and very small rates of O2 quenching indicate that these tryptophanyl side chains are embedded in a highly inflexible internal region of the macromolecule. Aided by sequence homology with dehydrogenases of known structure and theoretical predictions of secondary structure [Wootton, J.C. (1974) Nature (London) 252, 542-546; Brett, M., Chambers, G.K., Holder, A. A., Fincham, J.R.S., & Wootton, J.C. (1976) J. Mol. Biol. 106, 1-22], the phosphorescing tryptophans have been tentatively placed in the catalytic coenzyme binding domain of each enzyme. The particular sensitivity of the triplet-state lifetime in probing local changes in conformation provides a strong indication that within the time window of phosphorescence measurements the six subunits in the hexameric enzymes are equivalent. Furthermore, while in the bovine enzyme this parameter is markedly affected by the interaction with ligands which have a functional role, the constancy of the phosphorescence lifetime at various degrees of polymerization suggests that the association process is not accompanied by important conformational changes in the macromolecule.
Asunto(s)
Glutamato Deshidrogenasa/metabolismo , Triptófano , Alcohol Deshidrogenasa/metabolismo , Animales , Bovinos , Caballos , Cinética , Hígado/enzimología , Mediciones Luminiscentes , Conformación Proteica , Espectrometría de Fluorescencia , TermodinámicaRESUMEN
Pulse radiolysis and steady-state X-radiolysis have been used to investigate the radiation inactivation of aldolase from rabbit muscle. Both eaq-and OH readily react with aldolase, and contribute to inactivation. The radical anions (CNS)2-and (Br)2-react with aldolase at neutral pH. The progressive addition of alkali results in an increase in the second-order rate constants, with an apparent pK approximately 10 +/- 0-3, and with the formation of an unstable intermediate, lambdamax approximately 400 nm resembling a phenoxyl radical. Steady-state radiolysis in the presence of (CNS)2-and (Br)2- at alkaline pH results in increased aldolase inactivation, with a pK of enzyme inactivation similar to that observed for reaction of the radical anions. We propose that a reaction of the radical anoins with tyrosine residues accounts for the resultant inactivation.
Asunto(s)
Fructosa-Bifosfato Aldolasa/efectos de la radiación , Músculos/enzimología , Efectos de la Radiación , Animales , Relación Dosis-Respuesta en la Radiación , Concentración de Iones de Hidrógeno , Técnicas In Vitro , Conejos , Rayos XRESUMEN
Two polypeptides with antiproteolytic activities have been isolated from alfalfa leaves. Polypeptide I resembles the previously described plant protease inhibitors in both structural and functional features; it has a molecular weight of 15,000, a random coil secondary structure, and inhibits exogenous protease as well as alfalfa leaf protease. Polypeptide II is a novel type of plant inhibitor with a molecular weight of 6300 and a highly organized structure with a high (40-50%) alpha-helix content. It only inhibits endogenous protease with a molar stoichiometry polypeptide/enzyme protein of 1.
Asunto(s)
Medicago sativa/análisis , Proteínas de Plantas/aislamiento & purificación , Inhibidores de Proteasas/aislamiento & purificación , Aminoácidos/análisis , Fenómenos Químicos , Química , Cromatografía/métodos , Dicroismo Circular , Concentración de Iones de Hidrógeno , Medicago sativa/enzimología , Peso Molecular , Proteínas de Plantas/farmacología , Conformación ProteicaRESUMEN
The inhibition of a highly purified alfalfa (Medicago sativa) leaf protease by naturally occurring polyamines is reported. The tetraamine spermine shows the highest inhibitory effect, with the maximum inhibition at 0.1 mM. Kinetic data indicate an apparent hyperbolic competitive inhibition. CD measurements show that in the presence of 0.1 mM spermine the enzyme undergoes a conformational change with the loss of 16% alpha-helix secondary structure content. Both the inhibition and the conformational change are prevented by high ionic strength. These data suggest a novel control mechanism of proteolytic activity in the leaf.