Coastal degradation impacts on green turtle's (Chelonia mydas) diet in southeastern Brazil: Nutritional richness and health.

This study evaluated the influence of environmental degradation on the nutritional value of the main marine macrophytes consumed by green sea turtles (Chelonia mydas) in areas with different degrees of urbanization. Macrophyte assemblages in the highly urbanized area (HUa) showed lower richness compared to the lightly urbanized area (LUa) (Mann-Whitney U test: 10.0 ± 3.6 SD genera and 11.9 ± 4.2 taxa per transect vs. 20.1 ± 7.0 genera and 23.5 ± 9.2 taxa per transect) respectively. Also, diet was poorer with 4.0 ± 1.6 genera per turtle (vs. 8.5 ± 4.0 in HUa) and less diverse with Shannon index of diversity = 0.45 ± 0.29 (vs. 0.64 ± 0.46 in LUa). Body condition was similar in both areas. About half of individuals were classified as having normal body condition, 14-15% as underweight and 23-34% as being emaciated. Fibropapillomatosis prevalence (χ2 = 8.720; n = 222; df = 1; p = 0.003) was higher in the HUa but, in affected animals, severity was marginally non-significant (χ2 = 5.721; n = 82; df = 2; p = 0.057). Significant differences in energy content (kcal) were detected between areas in both summer (S) and winter (W). All ANOVAs on total lipids (F = 22.15 [S] and 30.39 [W]), total water-soluble proteins (F = 327.65 [S] and 64.42 [W]) and total carbohydrates (F = 70.90 [S] and 27.62 [W]) showed high significance (p < 0.001). Carotenoids concentration yielded significant results for Halodule in summer and Hypnea in winter (ANOVAs, F = 39.42 and 13.07, respectively). For both, tests revealed that concentration was higher in LUa than HUa. High levels of phycobiliproteins and proteins in this area probably reflect nitrogen accumulation. Frequency and severity of fibropapillomatosis suggest that urbanization-caused alterations in species diversity and in chemical composition of marine plants affect green turtles' health. LIGHT ABSTRACT: The use of coastal areas by humanity is widespread and increasing. The impacts caused to the coastal environment, be it terrestrial, estuarine or marine, are important and affect numerous species. Our study evaluated the influence of environmental degradation on the nutritional value of the main algae eaten by the green turtle, one of the very few marine megaherbivores (those herbivores with body mass above 10 kg). Diet in the highly urbanized area was richer in proteins, lipids and carbohydrates (sugars) and lower in carotenoids (photosynthetic and photoprotectant pigments in algae and plants; precursors of vitamin A involved in oxygen transport in animals-animals do not synthetize such molecules). High levels in phycobiliproteins (photosynthetic pigments present in some algae) and proteins in the highly urbanized area probably result from organic pollution and nitrogen accumulation in coastal waters. Nitrogen compounds dissolved in water are a threat to vertebrates due to its toxicity and negative effects on the immune system. Our results suggest that algae chemical composition and severity of fibropapillomatosis (tumors caused by a herpesvirus in green sea turtles) are directly related through environmental alterations caused by urbanization.

Ecological thresholds of periphytic communities and ecosystems integrity in lower Doce River basin.

Freshwater biodiversity has been impacted by several stressors such as eutrophication, turbidity and metals. Besides these frequent impacts, large-scale accidents occasionally affect aquatic systems, input an intense load of contaminants to the water bodies, as in the case of the Fundão tailing dam collapse (Brazil), which launched millions of meters cubic of iron ore tailing in the Doce River Basin. Our aim in this study was to assess how much stress the environmental conditions of lentic and lotic environments in the lower region of this basin impact the periphytic community. In addition, we intend to verify whether the limits of changes of the periphytic communities agreed with Brazilian legislation for the protection of aquatic life. For that, monthly samplings were carried out in seven sampling stations in lentic environments and five in lotic environments, including the Doce River channel, between October/2018 and March/2021 (except in October/2019). Concentrations of metals (iron, aluminum, manganese and chromium), total nitrogen, total phosphorus, conductivity, and suspended particulate material were determined. The periphytic community was collected from natural substrates (macrophytes or pebbles) and quantified by the sedimentation chamber method. From these data, thresholds of concentrations of variables that caused changes in periphytic communities were determined by the Threshold Indicator Taxa ANalysis (TITAN). We concluded that evidence of chronic impact of iron ore tailing should be revealed and that the standards established by Brazilian legislation may be incompatible with the protection of aquatic life. Moreover, we found that the Doce River mouth and surrounding environments were the most stressful environments for the periphytic communities.

Effects of intensive fish-farming and domestic wastewater on the periphytic algal community in a tropical coastal lagoon (Juara, Brazil) / Efeitos da piscicultura intensiva e de efluentes domésticos na comunidade de algas perifíticas em uma lagoa costeira tropical (Juara, Brasil)

This study aimed at evaluating the effects of the intensive fish-farming and the domestic wastewater on the structure of the phycoperiphyton community. Three experimental supports containing artificial substrate were assembled in two sampling sites: IF ­ region with intensive fish-farming and DW ­ near the domestic wastewater discharge. Samplings were carried out after the 21st, 26th and 31st day of colonization. The abiotic variables evaluated were: transparency, electrical conductivity, pH, turbidity, total suspended solids, alkalinity, dissolved oxygen, water temperature and nutrients. Phycoperiphyton was examined with regard to density, richness, abundance, dominance, diversity and eveness. Nutrients (total nitrogen, nitrate, ammonia nitrogen, orthophosphate and silicate) were different among the sampling sites. Although the total density, richness, diversity and evenness of phycoperiphyton were not affected by the different impacts, the density by class had differences between the sampling sites, with Bacillariophyceae presenting higher density at IF and Cyanophyceae and Coscinodiscophyceae at DW. Four taxa have been associated with the impact by domestic wastewater, and four, to the intensive fish-farming. The density by class of phycoperiphyton was the best attribute to evaluate the effects of human activity.

A presente pesquisa visou avaliar o efeito da piscicultura intensiva e de esgoto doméstico na estrutura da comunidade ficoperifítica. Foram implantados três suportes experimentais contendo substratos artificiais em duas estações amostrais da lagoa Juara: IF - região de piscicultura intensiva e DW - próxima ao lançamento de efluentes domésticos. As coletas foram realizadas após 21, 26 e 31 dias de colonização. As variáveis abióticas avaliadas foram transparência, condutividade elétrica, pH, turbidez, sólidos totais em suspensão, alcalinidade, oxigênio dissolvido, temperatura da água e nutrientes. As algas perifíticas foram analisadas pelos atributos: densidade, riqueza, abundância, dominância, diversidade e equitabilidade. As concentrações de nutrientes (nitrogênio total, nitrato, nitrogênio amoniacal, ortofosfato e silicato) diferiram entre as duas estações amostrais. Apesar da densidade total, riqueza, diversidade e equitabilidade não diferirem em relação às estações amostrais, a densidade por classes apresentou diferenças com Bacillariophyceae, tendo maior densidade em IF e Cyanophyceae e Coscinodiscophyceae em DW. Quatro táxons estiveram relacionados com o impacto por efluente doméstico e quatro com a piscicultura intensiva. A densidade por classes do ficoperifíton mostrou ser o melhor atributo quando se deseja avaliar o efeito das atividades antrópicas.

Spatial variation of periphyton structural attributes on Eichhornia crassipes (Mart) Solms in a tropical lotic ecosystem / Variações espaciais dos atributos estruturais do perifíton em Eichhornia crassipes (Mart) Solms em um ecossistema lótico tropical

This study evaluated the influence of physical, chemical and physico-chemical variables of water on the biomass of periphyton community and verified the differences between six sampling sites over the course of São Mateus river: two upstream of the city of São Mateus, Espírito Santo State (E1, E2), two along (E3, E4), and two downstream of the city (E5, E6). The periphyton was collected from roots of Eichhornia crassipes (Mart.) Solms. Samplings were undertaken every week in September and October 2010. The periphyton biomass was estimated through chlorophyll 'a', biovolume, dry mass, ash-free dry mass, and ash. Higher values of chlorophyll 'a' were found at E1, while the total biovolume featured greater values in E4 and E3. Regarding the values of periphyton dry mass, the inorganic fraction was higher at sites along and downstream of the city of São Mateus. The variation of periphyton biomass was influenced by the availability of nutrients (phosphorus and nitrogen) and turbidity, as evidenced by the CCA. The results suggest that the input of allochthonous material, especially from human activities (fish farming and discharge of domestic and industrial wastewater), has changed the water quality (as pointed out by the PCA), as well as the communities present.

Este estudo avaliou a influência das variáveis físicas, químicas e físico-químicas da água sobre a biomassa da comunidade perifítica e verificou as diferenças entre seis estações amostrais ao longo do rio São Mateus: duas a montante da cidade de São Mateus, Estado do Espírito Santo (E1, E2), duas ao longo (E3, E4) e duas a jusante da cidade (E5, E6). O perifíton foi coletado de raízes de Eichhornia crassipes (Mart.) Solms. As amostragens foram realizadas em intervalos semanais, em setembro e outubro/2010. A biomassa perifítica foi estimada por meio da clorofila a, biovolume, massa seca, massa seca livre de cinzas e cinzas. Maiores valores de clorofila foram registrados em E1, enquanto o biovolume total apresentou valores mais elevados em E4 e E3. Quanto aos valores de massa seca perifítica, a parte inorgânica foi mais elevada, nas estações ao longo e a jusante da cidade. A variação da biomassa perifítica foi influenciada pela disponibilidade de nutrientes (fósforo e nitrogênio), assim como pela turbidez, como constatado pela CCA. Os resultados sugerem que a entrada de material alóctone, proveniente principalmente das atividades antrópicas (piscicultura e lançamento de efluentes domésticos e industriais) alteram a qualidade da água (como evidenciado na PCA), assim como as comunidades presentes.