Process bioengineering applied to BTEX degradation in microaerobic treatment systems.

The effect of different microaeration flow rates and dosing points, and of effluent recirculation, on microaerobic BTEX degradation in an anaerobic bioreactor was assessed. Additionally, a sensitivity and recovery analysis for this system was performed during microaeration failure simulations. Under anaerobic conditions, BTEX removal efficiencies between 55 and 82% were achieved depending on the compound, being benzene the most recalcitrant one. Microaeration (0.5-2.0 mL air min-1) ensured high removal efficiencies (>83%) for all compounds, and the best results were obtained for the flow rate of 1.0 mL air min-1, particularly for benzene, with a 30% increase in its removal efficiency. Effluent recirculation showed to be an important factor to improve mass transfer and, consequently, BTEX removal. Volatilization was negligible even under microaerobic conditions, suggesting that microbial activity was the main removal mechanism. Finally, after microaeration shutdown periods, the bioreactor could recover its prior performance within up to 2 days.

Multivariate optimization of headspace-GC for the determination of monoaromatic compounds (benzene, toluene, ethylbenzene, and xylenes) in waters and wastewaters.

The objective of this study was to optimize, by employing a central composite rotatable design, and validate an analytical method to detect and quantify monoaromatic compounds (benzene, toluene, ethylbenzene, and xylenes) in waters and wastewaters by using headspace extraction followed by GC coupled with photoionization detection. The extraction parameters optimized were: salinity, sample volume, incubation time, and extraction temperature. The results revealed that the sample volume was the most significant parameter in the extraction process, whereas the salinity effect was negligible, which extends the applicability of the analytical method to waters with different salinities. Finally, the studied method was very selective and, at the optimal extraction conditions (15 mL sample volume, 15 min incubation time, and temperature of 70°C), presented excellent repeatability (<4%), linearity (R > 0.999 for each compound), and sensitivity, since very low LODs (0.13-0.48 µg/L) and LOQs (0.43-1.61 µg/L) were achieved.

Applicability of Microaerobic Technology to Enhance BTEX Removal from Contaminated Waters.

As the addition of low concentrations of oxygen can favor the initial degradation of benzene, toluene, ethylbenzene, and xylenes (BTEX) compounds, this work verified the applicability of the microaerobic technology to enhance BTEX removal in an anaerobic bioreactor supplemented with high and low co-substrate (ethanol) concentrations. Additionally, structural alterations on the bioreactor microbiota were assessed throughout the experiment. The bioreactor was fed with a synthetic BTEX-contaminated water (~ 3 mg L-1 of each compound) and operated at a hydraulic retention time of 48 h. The addition of low concentrations of oxygen (1.0 mL min-1 of atmospheric air at 27 °C and 1 atm) assured high removal efficiencies (> 80%) for all compounds under microaerobic conditions. In fact, the applicability of this technology showed to be viable to enhance BTEX removal from contaminated waters, especially concerning benzene (with a 30% removal increase), which is a very recalcitrant compound under anaerobic conditions. However, high concentrations of ethanol adversely affected BTEX removal, especially benzene, under anaerobic and microaerobic conditions. Finally, although bacterial community richness decreased at low concentrations of ethanol, in general, the bioreactor microbiota could deal with the different operational conditions and preserved its functionality during the whole experiment.

Occurrence and removal of estrogens in Brazilian wastewater treatment plants.

This paper evaluated the occurrence and removal efficiency of four estrogenic hormones in five biological wastewater treatment plants (WWTPs), located in the State of Ceará, Brazil. The five WWTPs comprised: two systems consisted of one facultative pond followed by two maturation ponds, one facultative pond, one activated sludge (AS) system followed by a chlorination step, and one upflow anaerobic sludge blanket (UASB) reactor followed by a chlorination step. Estrogen occurrence showed a wide variation among the analyzed influent and effluent samples. Estrone (E1) showed the highest occurrence in the influent (76%), whereas both 17ß-estradiol (E2) and 17α-ethynylestradiol (EE2) presented a 52% occurrence, and the compound 17ß-estradiol 17-acetate (E2-17A), a 32% one. The occurrence in the effluent samples was 48% for E1, 28% for E2, 12% for E2-17A, and 40% for EE2. The highest concentrations of E1 and EE2 hormones in the influent were 3050 and 3180 ng L(-1), respectively, whereas E2 and E2-17A had maximum concentrations of 776 and 2300 ng L(-1), respectively. The lowest efficiencies for the removal of estrogenic hormones were found in WWTP consisted of waste stabilization ponds, ranging from 54 to 79.9%. The high-rate systems (AS and UASB), which have chlorination as post-treatment, presented removal efficiencies of approximately 95%.

Colour removal of dyes from synthetic and real textile wastewaters in one- and two-stage anaerobic systems.

Decolourisation of the azo dye model compound, Congo Red (CR), and real textile wastewater, was assessed in one- and two-stage anaerobic treatment systems (R1 and R2, respectively). High colour removals were achieved in both treatment systems even when a very high CR concentration (1.2 mM) was applied. However, R2 presented a slightly better stability, in which the acidogenic reactor (R(2,A)) played a major role on dye reduction, as compared to the methanogenic reactor (R(2,M)), evidencing the role of fermentative microorganisms. The minimum electron donor concentration required to sustain dye reduction was much higher than the stoichiometric amount. Additionally, a decrease on the hydraulic retention time (from 24 to 12 h) did not significantly affect decolourisation, indicating that electron transfer was not a concern. Finally, experiments with real textile wastewater showed low decolourisation efficiencies in both systems, most likely due to the presence of dyes not susceptible to reductive decolourisation under these experimental conditions.