Perceptions of the rabbit as a low investment 'starter pet' lead to negative impacts on its welfare: Results of two Danish surveys.

Concerns over compromised companion rabbit (Oryctolagus cuniculus domesticus) welfare are widespread. The welfare problems have been linked to the perception of rabbits as low investment 'children's pets.' To test this hypothesis and investigate the current conditions for rabbits, data were gathered from two surveys in 2021: a nationally representative survey of Danish companion animal owners (Survey I) and a detailed social media-based survey of Danish rabbit owners (Survey II). Using logistic regression, three owner-related variables (whether a child/adult was responsible for care of the rabbit, owner-opinion on rabbits' suitability as 'starter pets' and willingness-to-pay [WTP] for veterinary treatment) were employed to investigate the effect of rabbit status on owner-provision of selected husbandry conditions. The 76 (Survey I) and 4,335 (Survey II) responses suggested that most rabbits are acquired for children and are solitarily housed, and that many are kept in cages of an unsuitable size and not checked daily. Owners who perceived rabbits as 'starter pets' and with lower WTP were more likely to house rabbits in restricted space and to not provide continuous gnawing opportunities, ad libitum hay or routine healthcare. A child fulfilling the role of the rabbit's main caretaker was also associated with inadequate housing type and fewer gnawing opportunities. Thus, many rabbits live in unsuitable conditions, and owners who perceive rabbits as low investment 'children's pets' are more likely to not provide recommended resources. Changing owners' perceptions of rabbits and promoting suitable husbandry through official education programmes and minimum requirements is important if there are to be improvements made to rabbit welfare.

Identifying areas of animal welfare concern in different production stages in Danish pig herds using the Danish Animal Welfare Index (DAWIN).

Animal welfare is of increasing public interest, and the pig industry in particular is subject to much attention. The aim of this study was to identify and compare areas of animal welfare concern for commercial pigs in four different production stages: (1) gestating sows and gilts; (2) lactating sows; (3) piglets; and (4) weaner-to-finisher pigs. One welfare assessment protocol was developed for each stage, comprising of between 20 and 29 animal welfare measures including resource-, management- and animal-based ones. Twenty-one Danish farms were visited once between January 2015 and February 2016 in a cross-sectional design. Experts (n = 26; advisors, scientists and animal welfare controllers) assessed the severity of the outcome measures. This was combined with the on-farm prevalence of each measure and the outcome was used to calculate areas of concern, defined as measures where the median of all farms fell below the value defined as 'acceptable welfare.' Between five and seven areas of concern were identified for each production stage. With the exception of carpal lesions in piglets, all areas of concern were resource- and management-based and mainly related to housing, with inadequate available space and the floor type in the resting area being overall concerns across all production stages. This means that animal-based measures were largely unaffected by perceived deficits in resource-based measures. Great variation existed for the majority of measures identified as areas of concern, demonstrating that achieving a high welfare score is possible in the Danish system.

Growth rate and behaviour in separated, partially separated or non-separated kids and the corresponding milk production of their mothers.

We address the hypothesis that keeping kids and mothers together would have positive effects on the milk composition of the mother and the behaviour of the kids. Kids were either permanently separated (SEP), daily separated between 7.30 and 15 h (DAY-SEP) or kept with mothers 24 h/d (NON-SEP). The NON-SEP kids were only allowed to suckle one teat. All kids had similar growth rate throughout the study (lactation days 5-70). DAY-SEP kids spent 24% of their time with their mother at both ages. NON-SEP spent only 15% of the time with their mothers at 2 weeks of age and this increased to 28% at 2 months of age. NON-SEP kids showed more hiding behaviour at 2 weeks and SEP were more active alone, at both 2 weeks and 2 months, compared to the other treatments. The mean available milk yield and fat concentration were higher in DAY-SEP goats (2420 g ± 119 g and 4.9 ± 0.1%) compared with NON-SEP goats (2149 ± 79 g and 4.4 ± 0.1%). There were no differences between DAY-SEP and NON-SEP goats in total protein, lactose, or casein concentrations. Based on these data it was estimated that 7.1 kg milk was needed to produce 1 kg semi-hard cheese in DAY-SEP goats and 7.5 kg in NON-SEP goats, respectively. When comparing milk yield and composition between udder halves, the milk yield was, as expected, higher from the machine milked teat than from the suckled one in the NON-SEP goats but there was no difference between right and left udder halves in DAY-SEP goats. Milk fat concentration varied between teats at morning and afternoon milkings in NON-SEP goats, but there was no difference in milk fat between udder-halves in DAY-SEP goats. In conclusion, the kid growth rate was similar in all treatments, however, an altered behaviour was seen in permanently separated kids (SEP). The results show that it is possible to have a high milk yield and fat concentration with one kid together with the dam.

An equine pain face.

OBJECTIVE: The objective of this study was to investigate the existence of an equine pain face and to describe this in detail. STUDY DESIGN: Semi-randomized, controlled, crossover trial. ANIMALS: Six adult horses. METHODS: Pain was induced with two noxious stimuli, a tourniquet on the antebrachium and topical application of capsaicin. All horses participated in two control trials and received both noxious stimuli twice, once with and once without an observer present. During all sessions their pain state was scored. The horses were filmed and the close-up video recordings of the faces were analysed for alterations in behaviour and facial expressions. Still images from the trials were evaluated for the presence of each of the specific pain face features identified from the video analysis. RESULTS: Both noxious challenges were effective in producing a pain response resulting in significantly increased pain scores. Alterations in facial expressions were observed in all horses during all noxious stimulations. The number of pain face features present on the still images from the noxious challenges were significantly higher than for the control trial (p = 0.0001). Facial expressions representative for control and pain trials were condensed into explanatory illustrations. During pain sessions with an observer present, the horses increased their contact-seeking behavior. CONCLUSIONS AND CLINICAL RELEVANCE: An equine pain face comprising 'low' and/or 'asymmetrical' ears, an angled appearance of the eyes, a withdrawn and/or tense stare, mediolaterally dilated nostrils and tension of the lips, chin and certain facial muscles can be recognized in horses during induced acute pain. This description of an equine pain face may be useful for improving tools for pain recognition in horses with mild to moderate pain.

Handheld mechanical nociceptive threshold testing in dairy cows - intra-individual variation, inter-observer agreement and variation over time.

OBJECTIVE: To examine the use of handheld methodology to assess mechanical nociceptive threshold (MNT) on cows kept loose-housed. STUDY DESIGN: Prospective randomized partial cross-over experimental study. A one-factor (test day) design was used to evaluate MNT over time. ANIMALS: One hundred and fifteen healthy, loose-housed Danish Holstein cattle. METHODS: We evaluated intra-individual variation, inter-observer agreement and variation over time of MNT using two handheld devices and two stimulation sites. Mechanical, ramped stimulations were performed with an algometer (6.5 mm diameter steel probe, 0-10.0 kgf) or an electronic von Frey device (plastic tip with diameter 0.8 mm, 0-1000 gf). Each cow received 5-6 consecutive stimulations within a 2 × 5 cm skin area on the dorsal or lateral aspect of the left third metatarsus until an avoidance reaction occurred. We investigated the difference in precision [expressed as coefficient of variation (CV)] between the combinations of devices and stimulation sites. The inter-observer agreement and the difference in MNT between test day 1, 3, 7, 10 and 24 were investigated for selected combinations. Data were analysed in mixed models and Bland-Altman as relevant. RESULTS: The CVs did not differ [range 0.34-0.52 (p = 0.1)]. Difference between observers (95% limits) was 0.2 kgf (2.8) and 4 gf (369) for the algometer and von Frey device, respectively. Mechanical nociceptive threshold increased from 361 on test day one to 495 gf on test day 24 (p < 0.01). CONCLUSION AND CLINICAL RELEVANCE: All methods showed a high degree of intra-individual variation, and no combination of device and stimulation site showed superior precision. Mean difference between observers was low, and MNT was not consistent over time. Further development of the methods is required before they can be used in research to investigate possible relations between claw lesions and hyperalgesia.

Scientific and technical assistance on welfare aspects related to housing and health of cats and dogs in commercial breeding establishments.

This Scientific Report addresses a mandate from the European Commission according to Article 31 of Regulation (EC) No 178/2002 on the welfare of cats and dogs in commercial breeding establishments kept for sport, hunting and companion purposes. The aim was to scrutinise recent recommendations made by the EU Platform on Animal Welfare Voluntary Initiative on measures to assist the preparation of policy options for the legal framework of commercial breeding of cats and dogs. Specifically, the main question addressed was if there is scientific evidence to support the measures for protection of cats and dogs in commercial breeding related to housing, health considerations and painful procedures. Three judgements were carried out based on scientific literature reviews and, where possible a review of national regulations. The first judgement addressed housing and included: type of accommodation, outdoor access, exercise, social behaviour, housing temperature and light requirements. The second judgement addressed health and included: age at first and last breeding, and breeding frequency. Judgement 3 addressed painful procedures (mutilations or convenience surgeries) and included: ear cropping, tail docking and vocal cord resections in dogs and declawing in cats. For each of these judgements, considerations were provided indicating where scientific literature is available to support recommendations on providing or avoiding specific housing, health or painful surgical interventions. Areas where evidence is lacking are indicated.

Erratum: Identifying areas of animal welfare concern in different production stages in Danish pig herds using the Danish Animal Welfare Index (DAWIN) - CORRIGENDUM.

[This corrects the article DOI: 10.1017/awf.2023.37.].

Market driven initiatives can improve broiler welfare - a comparison across five European countries based on the Benchmark method.

Two kinds of initiatives exist to ensure welfare in broiler production: welfare legislation, where all broiler production in a country or region must comply with legally defined welfare standards; and market driven initiatives, where part of the production must meet specific welfare standards and is sold with a particular label, typically at a price premium, or as part of minimum welfare standards defined by a retailer, a fast-food chain or the like. While the effects of national legislation may be undermined by price competition from lower welfare imported products, the effects of market driven initiatives may be limited by lack of willingness from consumers to pay the extra cost. To investigate how this works out in practice, we compared broiler welfare requirements in 5 European countries, Denmark, Germany, United Kingdom, the Netherlands, and Sweden, in 2018, by means of the Benchmark method. A number of welfare dimensions, covering the input features typically modified in broiler welfare initiatives, were defined. A total of 27 academic welfare experts (response rate 75%) valued the different levels within each dimension on a 0 to 10 scale, and then weighted the relative contribution of each dimension to overall welfare on a 1 to 5 scale. By combining these values and weights with an inventory of existing welfare initiatives, the additional welfare generated by each initiative was calculated. Together with information on national coverage of each initiative, the Benchmark score for each country's production and consumption of chicken meat was calculated. Sweden achieved a much higher Benchmark for national production due to higher legal standards than any of the four other countries. The Netherlands, on the other hand, achieved a Benchmark for national consumption of chicken at the same level as that found in Sweden, because market driven initiatives complemented more welfare-limited Dutch legislation. So, despite some uncertainties in the Benchmark method, it appears that market driven initiatives can have a strong impact on improving broiler welfare, building on those standards achieved by animal welfare legislation.

The Effect of Mother Goat Presence during Rearing on Kids' Response to Isolation and to an Arena Test.

The aim of the study was to examine how early permanent separation, separation during the day only, or full-time access to the mother goat affected goat kids during social isolation with a sudden sound of a dog bark at two weeks and two months, and a novel arena test with a novel object at two months. Kids permanently separated reduced their vocalization earlier and had a higher heart rate before and after dog bark during isolation at two weeks, no effect was found on the daytime separated kids. Daytime separated kids bleated more at two weeks and decreased heart rate after dog barking at two months. Daytime separated kids showed the strongest fear reaction in the arena test, no effect was found on the permanently separated kids. Kids separated early vocalized more before novel object and showed more explorative behavior afterwards. Our study shows different responses in goat kids separated early permanent, daytime separated, or kept full-time with mother, which demonstrates the importance of if and how the mother is present, and the impact of using a wide variety of physiological and behavioral measures when evaluating stress in animal welfare research.

Comparing Behavioural Problems in Imported Street Dogs and Domestically Reared Danish Dogs-The Views of Dog Owners and Veterinarians.

Street dogs are common in southern and eastern parts of Europe. They are often adopted by people living in North European countries, including Denmark. However, these dogs may experience difficulties adjusting to their new life as companion animals, and this may in turn lead to behavioural problems and complications for owners. The objective of this study was therefore to investigate whether former street (FS) dogs display a higher degree of behavioural problems than dogs reared in Denmark (RD). Two questionnaires were developed. One was distributed to Danish dog owners and resulted in 3020 useful answers. FS dogs were found to display 9 of the 45 listed behaviours more often than RD dogs. All of these behaviours were related to fear, aggression and stress. The second questionnaire was distributed to Danish veterinarians working in small animal practices and resulted in 173 useful answers. The most commonly reported behavioural problems were fear of humans, stress and problems when the dog was left at home alone. The extent of the behavioural problems reported by the veterinarians was much greater than that reported by the dog owners which, at least partly, may be due to fear-induced reactions of the dogs when handled at the veterinary clinic.

Regulating Companion Dog Welfare: A Comparative Study of Legal Frameworks in Western Countries.

There appear to be growing concerns among experts, NGOs, and members of the public about the welfare of companion dogs. With farm and laboratory animals, legislative initiatives have long been considered valuable tools in the management of welfare whereas the use of legislation to protect companion animal welfare has received less attention. We aim to rectify this by comparing legislation with an impact on the welfare of companion dogs in eleven Western jurisdictions. The comparison also provides a basis for further consideration of regulatory initiatives. We identify the rules applying in the jurisdictions and classify them in accordance with the following categories: breeding of dogs with risks to the health of the offspring, reproductive limitations, sales, surgical interventions, day-to-day handling, and killing. We demonstrate that, overall, there is significant variation across the jurisdictions. However, the degree of variation depends on the specific category. Whereas most countries, with the USA being a notable exception, regulate sales of dogs and ban surgical interventions, there is considerable variation in the regulation of day-to-day handling and the killing of dogs. Furthermore, different jurisdictions employ different regulatory tools to ensure the desired level of welfare for companion dogs. Overall, there appears to be real potential for dialogue and mutual inspiration.

A Sustainability Compass for policy navigation to sustainable food systems.

Growing acknowledgement that food systems require transformation, demands comprehensive sustainability assessments that can support decision-making and sustainability governance. To do so, assessment frameworks must be able to make trade-offs and synergies visible and allow for inclusive negotiation on food system outcomes relevant to diverse food system actors. This paper reviews literature and frameworks and builds on stakeholder input to present a Sustainability Compass made up of a comprehensive set of metrics for food system assessments. The Compass defines sustainability scores for four societal goals, underpinned by areas of concern. We demonstrate proof of concept of the operationalization of the approach and its metrics. The Sustainability Compass is able to generate comprehensive food system insights that enables reflexive evaluation and multi-actor negotiation for policy making.

Application of Methods to Assess Animal Welfare and Suffering Caused by Infectious Diseases in Cattle and Swine Populations.

Control of infectious diseases in livestock has often been motivated by food safety concerns and the economic impact on livestock production. However, diseases may also affect animal welfare. We present an approach to quantify the effect of five infectious diseases on animal welfare in cattle (three diseases) and pigs (two diseases). We grouped clinical manifestations that often occur together into lists of clinical entities for each disease based on literature reviews, and subsequently estimated "suffering scores" based on an aggregation of duration, frequency, and severity. The duration and severity were based on literature reviews and expert knowledge elicitation, while frequency was based mainly on estimates from the literature. The resulting suffering scores were compared to scores from common welfare hazards found under Danish conditions. Most notably, the suffering scores for cattle diseases were ranked as: bovine viral diarrhoea and infection with Mycobacterium avium subsp. paratuberculosis > infectious bovine rhinotracheitis, and for pigs as: porcine respiratory and reproductive syndrome > Aujeszky's disease. The approach has limitations due to the limited data available in literature and uncertainties associated with expert knowledge, but it can provide decision makers with a tool to quantify the impact of infections on animal welfare given these uncertainties.

The effect of age when group housed and other management factors on playing and non-nutritive sucking behaviour in dairy calves: a cross sectional observational study.

BACKGROUND: The aim of this study was to investigate if calves' play behaviour and non-nutritive sucking behaviour, as indirect measures of welfare status, are associated with the age of the calf when group housed, age when observed, age difference within the group, pen size, milk feeding system, current or previous sicknesses, access to dry teat, indoor/outdoor rearing, sex, organic/conventional farm, group size and regrouping events. An observational study was conducted on 176 Danish dairy calves in the age range of 1-12 weeks, on both conventional (n = 17) and organic (n = 5) farms. All calves had been group housed before 8 weeks of age and had spent various periods of time with the dam and/or individually housed before being group housed. Behaviour was recorded continuously by filming each individual calf over a period of 30 min. RESULTS: The calf's age when group housed for the first time was not found to be significantly associated with duration of either play behaviour (P = 0.55) or non-nutritive sucking behaviour (P = 0.44). It was found that calves had significantly reduced odds of playing for longer than the mean play duration (5.5 s) for each day of their lives (OR = 0.97, P = 0.003). Also, they had reduced odds of performing non-nutritive sucking behaviour for longer than the mean non-nutritive sucking duration (145.5 s) when milk was allocated by drinker buckets fitted with a teat compared to by bowl or trough (OR = 0.06, P = 0.02). CONCLUSION: No significant associations were found between calves' age when group housed for the first time and play and non-nutritive sucking behaviour. It was found that calves' play behaviour decreased with increasing age, and that non-nutritive sucking behaviour decreased when milk was allocated with a teat compared to no teat.

Dairy cows with mild-moderate mastitis change lying behavior in hospital pens.

In dairy production, mastitis is a major problem affecting animal welfare, productivity, and economy. Hospital pens are typically not used for cows with mastitis, except for severe cases involving recumbency. This field trial included 47 cows from three Danish herds followed for 8 d, of which days 1-5 involved the experimental housing. After day 5, all cows were kept with the lactating group. We examined lying behavior in dairy cows with naturally occurring, mild-moderate mastitis in hospital pens [single or group (depending on conditions on the farm), all with deep straw bedding] vs. sick cows kept in the group of healthy herd mates. Within a herd, every other cow fulfilling the inclusion criteria regarding mastitis was allocated to each of the two experimental treatments. Clinical data from involved cases were collected. No significant differences between housing treatments were found in the clinical variables or the daily lying time. During the period of experimental housing, cows kept in hospital pens showed a higher frequency of lying bouts compared with control cows. This difference did not persist after reintroduction to the lactating herd mates. These results suggest that aspects of lying behavior of dairy cows with mastitis are sensitive to the environment as the frequency of lying bouts differed between cows kept in hospital pens and cows kept in control treatment. More controlled studies are needed to examine underlying motivations and evaluate consequences in terms of animal welfare. For such studies, the inclusion of healthy cows for comparison will be valuable.

Effects of environmental enrichment on health and bone characteristics of fast growing broiler chickens.

Providing environmental enrichment for broilers is a potential strategy to increase welfare, activity, and health. The aim of this study was to evaluate the effect of environmental enrichment on health and leg bone characteristics of broilers. One control and 8 types of enrichment were included: 2 distances between food and water (7 and 3.5 m), roughage, vertical panels, straw bales, 2 platforms (30 and 5 cm), and a lowered stocking density (34 kg/m2). Birds were kept according to conventional Danish guidelines. The study included 58 pens with approximately 500 birds each. On day 35 of age, 25 birds per pen were killed and included in a postmortem analysis of wooden breast, body condition scores, pathological conditions (femoral head necrosis, arthritis, tenosynovitis, fractures, tibial dyschondroplasia, and twisted tibiotarsus), muscle width of the lower leg, and tibiotarsus properties (bone strength, weight, length, and proximal diameter, middle diameter, and distal diameter). It was predicted that environmental enrichment would have a positive effect on pathology with the exceptions that environmental enrichment that increased activity would pose a risk factor for wooden breast development, and straw bales would be a risk factor for bacterial infections (arthritis, tenosynovitis, and femoral head necrosis). Furthermore, it was hypothesized that enriched groups would have increased muscle width, bone strength, and dimensions of the tibiotarsus. Broilers with 7 m between food and water had a longer distal diameter of the tibiotarsus than those with straw bales (P = 0.04). The birds provided with vertical panels had wider leg muscle than the treatments with roughage (P = 0.045), 3.5 m distance (P = 0.049), and straw bales (P = 0.044). No effects were found for the remaining outcomes. These results suggest that provision of vertical panels and increased distance between resources can result in larger muscle and bone dimension, possibly having a positive effect on leg health. Furthermore, the provision of environmental enrichment does not appear to be a risk factor for wooden breast or bacterial infection.

Benchmarking Farm Animal Welfare-A Novel Tool for Cross-Country Comparison Applied to Pig Production and Pork Consumption.

A pluralist approach to farm animal welfare, combining animal welfare legislation with market-driven initiatives, has developed in many countries. To enable cross-country comparisons of pig welfare, a number of welfare dimensions, covering the features typically modified in legislative and market-driven welfare initiatives aimed at pig production, were defined. Five academic welfare experts valued the different welfare states within each dimension on a 0-10 scale, then assessed the relative contribution of each dimension to overall welfare on a 1-5 scale. By combining these values and weights with an inventory of pig welfare initiatives in five countries, the additional welfare generated by each initiative was calculated. Together with information on the national coverage of each initiative, the Benchmark value for each country's production and consumption of pork could be calculated on a scale from 0 to 100. Two (Sweden and the UK) had a much higher Benchmark value than the rest. However, there was a drop in the Benchmark for consumption in Sweden and the UK (indicating imports from countries with lower-Benchmark values for production). Even though the experts differed in the values and weights ascribed to different initiatives, they were largely in agreement in their ranking of the countries.

The Effect of Straw, Rope, and Bite-Rite Treatment in Weaner Pens with a Tail Biting Outbreak.

Tail biting in pigs is an injurious behaviour that spreads rapidly in a group. We investigated three different treatments to stop ongoing tail biting outbreaks in 65 pens of 6-30 kg undocked pigs (30 pigs per pen; SD = 2): (1) straw (7 g/pig/day on the floor), (2) rope, and (3) Bite-Rite (a hanging plastic device with chewable rods). Pigs were tail scored three times weekly, until an outbreak occurred (four pigs with a tail wound; day 0) and subsequently once weekly. After an outbreak had occurred, a subsequent escalation in tail damage was defined if four pigs with a fresh tail wound were identified or if a biter had to be removed. Straw prevented an escalation better (75%) than Bite-Rite (35%; p < 0.05), and rope was intermediate (65%). Upon introduction of treatments (day 0), pigs interacted less with tails than before (day -1; p < 0.05). Behavioural observations showed that pigs engaged more with rope than Bite-Rite (p < 0.05). Bite-Rite pigs (but not straw or rope) increased their interaction with tails between day 0 and day 7 (p < 0.05). Straw was the most effective treatment. However, further investigations may identify materials or allocation strategies which are more effective still.

Assessment of positive emotions in animals to improve their welfare.

It is now widely accepted that good welfare is not simply the absence of negative experiences, but rather is primarily the presence of positive experiences such as pleasure. However scientific investigation of positive emotions has long been neglected. This paper addresses two main issues: first, it reviews the current state of scientific knowledge that supports the existence of positive affective states in animals and, second, it suggests possible applications of this knowledge that may enhance quality of life under animal management conditions. In the first part of the paper, recent advances in psychology and neuroscience are reviewed to provide pragmatic frameworks based on cognitive processes (such as positive anticipation, contrast and controllability) for further investigations of positive emotions in animals. Thereafter, the neurobiological bases of positive emotions are highlighted in order to identify behavioral and physiological expressions of positive experiences in animals. Monitoring both the autonomic nervous system (via heart rate and its variability) and the immune system could offer relevant tools to better assess emotional states in animals, complementary to classical adrenocortical measures. In the second part of the paper, useful strategies for enhancing positive experiences (such as physical, social and cognitive enrichment or putative genetic selection) are outlined. Then this paper emphasizes practical applications for assessing and promoting positive emotions that may help in providing animals with a better quality of life. Play, affiliative behaviors and some vocalizations appear to be the most promising convenient indicators for assessing positive experiences in laboratory and farm animals under commercial conditions.