RESUMEN
A plasmid, pRG1, has been constructed by incorporating the coding sequence of human hypoxanthine-guanine phosphoribosyltransferase (HPRT) into the expression vector pT7-7. Expression of human HPRT has been achieved in HPRT- Escherichia coli cells transformed with pRG1 and pGP1-2, as shown by: (1) exclusive labelling with [35S]methionine of a polypeptide with the same mobility as purified human HPRT on SDS-PAGE; and (2) measurement of HPRT activity after cell lysis. Although the majority of the recombinant HPRT was present in the particulate fraction after cell lysis and centrifugation, sufficient HPRT activity was present in the supernatant fraction to allow comparison with the HPRT purified from human erythrocytes and the activity in human haemolysates and lymphoblast lysates. Small differences in electrophoretic mobility on native gels were found between HPRT activity from these sources. The Km values of recombinant HPRT for the substrates 5-phospho-alpha-D-ribosyl-1-pyrophosphate and guanine were compared with those of lymphoblast and erythrocyte HPRT.
Asunto(s)
Escherichia coli/genética , Expresión Génica , Hipoxantina Fosforribosiltransferasa/genética , Cromatografía en Gel , Clonación Molecular , ADN/genética , Electroforesis en Gel de Poliacrilamida , Exones , Genes , Humanos , Hipoxantina Fosforribosiltransferasa/química , Hipoxantina Fosforribosiltransferasa/metabolismo , Cinética , Metionina/farmacología , Plásmidos , Proteínas Recombinantes/química , Proteínas Recombinantes/genética , Proteínas Recombinantes/metabolismo , Transformación BacterianaRESUMEN
Samples of 12 hospitalized Korsakoffs, 12 hospitalized non-Korsakoff alcoholics, and 12 controls, all groups being of comparable age range, were tested using a set of seven visual and olfactory measures which involved memory. By stepwise multiple discriminant analysis it was shown that strong separation could be effected between the three groups, solely on the basis of the olfactory recognition and recall tests, which included the Campbell-Gregson (1972) procedure. The use of of anosmia as a diagnostic indicator for Korsakoff's syndrome is discussed.
Asunto(s)
Trastorno Amnésico Alcohólico/psicología , Alcoholismo/psicología , Olfato/efectos de los fármacos , Adulto , Anciano , Trastorno Amnésico Alcohólico/diagnóstico , Alcoholismo/diagnóstico , Aprendizaje Discriminativo/efectos de los fármacos , Femenino , Humanos , Masculino , Recuerdo Mental/efectos de los fármacos , Persona de Mediana Edad , OdorantesRESUMEN
This study investigated the relationship between biological and psychological processes in the recovery phase of depression during treatment with cognitive therapy. In particular, we tested the hypothesis that biological and psychological processes are independent; the hypothesis that they are related in a linear or sequential process; and the hypothesis that there is a circular relationship between them. 35 depressed patients completed a 12 week program of cognitive therapy. Changes in measures of negative thinking and amine dysfunction over the course of therapy were compared for those who improved and those who did not. There was a significant relationship between improvement in symptoms and change for ATQ scores, DAS scores, and epinephrine levels. There was significant change in metanephrine levels during therapy which was not related to improvement. The changes were not consistent across indices. Although the results did not provide definite support for any of the four models of the relationship between biological and psychological variables during recovery from depression, the finding that there was some change in some of the indices of amine dysfunction during cognitive therapy indicates that the interface between biological and psychological processes in depression should continue to be studied.
Asunto(s)
Trastornos de Adaptación/terapia , Terapia Cognitivo-Conductual , Trastorno Depresivo Mayor/terapia , Trastorno Distímico/terapia , Epinefrina/orina , Metanefrina/orina , Adaptación Psicológica/fisiología , Trastornos de Adaptación/fisiopatología , Trastornos de Adaptación/psicología , Adulto , Anciano , Encéfalo/fisiopatología , Trastorno Depresivo Mayor/fisiopatología , Trastorno Depresivo Mayor/psicología , Trastorno Distímico/fisiopatología , Trastorno Distímico/psicología , Femenino , Humanos , Masculino , Persona de Mediana Edad , Norepinefrina/orina , Normetanefrina/orina , Pensamiento/fisiología , Resultado del TratamientoRESUMEN
A 12-session group program of cognitive therapy, designed by the first author, was evaluated with 35 persons suffering from major or minor depressive disorders. Effect-size scores were generated using the method of Nietzel, Russel, Hemmings, and Gretter (1987) and compared with cut-off points calculated using the method of Jacobson and Revenstorf (1988) and the norms established by Nietzel et al. (1987) in their meta-analysis. The effects of the Group Cognitive Therapy Program were found to be clinically significant according to the criteria of Jacobson and colleagues (Jacobson and Revenstorf, 1988; Jacobson, Follette and Revenstorf, 1984) for 73% of patients. It is concluded that the Group Cognitive Therapy Program is a clinically efficacious and cost-effective treatment for persons suffering from nonpsychotic, primary, unipolar depression.
Asunto(s)
Terapia Cognitivo-Conductual/métodos , Trastorno Depresivo/psicología , Trastorno Depresivo/terapia , Psicoterapia de Grupo/métodos , Estudios de Seguimiento , Humanos , Apego a Objetos , Autoimagen , Transferencia PsicológicaRESUMEN
Psychrotrophic lipolytic bacteria represent a significant problem in the storage of refrigerated dairy products. A lipase-encoding gene has been cloned and characterized from a highly lipolytic strain of Pseudomonas. The nucleotide sequence of the gene predicts a polypeptide of M(r) 49,905, which was identified when the gene was expressed in Escherichia coli.