A novel antibacterial and fluorescent coating composed of polydopamine and carbon dots on the surface of orthodontic brackets.

Many kinds of antibacterial coatings have been designed to prevent the adherence of bacteria onto the surface of a fixed orthodontic device of brackets. However, the problems such as weak binding force, undetectable, drug resistance, cytotoxicity and short duration needed to be solved. Thus, it has great value in developing novel coating methods with long-term antibacterial and fluorescence properties according to the clinical application of brackets. In this study, we synthesized blue fluorescent carbon dots (HCDs) using the traditional Chinese medicinal honokiol, which could cause irreversible killing effects on both gram-positive and gram-negative bacteria through positive charges on the surface and inducing reactive oxygen species (ROS) production. Based on this, the surface of brackets was serially modified with polydopamine and HCDs, taking advantage of the strong adhesive properties as well as the negative surface charge of polydopamine particles. It is found that this coating exhibits stable antibacterial properties in 14 days with good biocompatibility, which can provide a new solution and strategy to solve the series of hazards caused by bacterial adhesion on the surface of orthodontic brackets.

Zero Thermal Quenching Phenomenon of Green Emitting Carbon Dots with High Biocompatibility and Stable Multicolor Biological Imaging in a Hot Environment.

Carbon dots are emerging fluorescent nanomaterials with unique physical and chemical properties and a wide range of applications. Herein, we have designed and successfully synthesized thermally stable green emissive nitrogen-doped carbon dots (NCDs) with a photoluminescent quantum yield of 11.32% through facile solvent-free carbonization. NCDs demonstrated zero thermal quenching upon various temperatures modulating from 20 to 80 °C. The green emissive NCDs perform very stably even after heating them at 80 °C for 1 h. The thermal stability mechanism demonstrates that C═O and C═N functional groups control the particle aggregation and protect the fluorescent hub from photo-oxidation and thermal oxidation. Highly biocompatible CDs exhibit bright, stable, and multicolor emissions in T-ca cells under hot circumstances (25-45 °C). Additionally, NCDs offer long-term stability in the biosystem, as evidenced by the fact that the cell retains its brightness about 70% after prolonging the incubation time to 8 days. Furthermore, the fluorescent NCDs are utilized as in vivo imaging agents in the hot environment as they display bright and thermally stable imaging (27-45 °C) under 488 nm excitation. The results confirmed that the produced thermally stable NCDs could be used in biology and related medical fields that require hot environment imaging.

Design of carbon dots for bioimaging and behavior regulation of stem cells.

Carbon dots (CDs) have been widely used in bioimaging, biosensing and biotherapy because of their good biocompatibility, optical properties and stability. In this review, we comprehensively summarize the research on CDs in terms of synthesis methods, optical properties and biotoxicity. We describe and envisage the directions for CDs application in stem cell imaging and differentiation, with the aim of stimulating the design of future related CDs. We used 'carbon dots', 'stem cells', 'cell imaging', 'cell differentiation' and 'fate control' as keywords to search for important articles. The Web of Science database was used to extract vital information from a total of 357 papers, 126 review articles and 231 article proceedings within 12 years (2011-2022).

Carbon dots and composite materials with excellent performances in cancer-targeted bioimaging and killing: a review.

Carbon dots (CDs) are nanomaterials with excellent properties, including good biocompatibility, small size, ideal photoluminescence and surface modification, and are becoming one of the most attractive nanomaterials for the imaging, detection and treatment of tumors. Based on these advantages, CDs can be combined other materials to obtain composite particles with improved, even new, performance, mainly in photothermal and photodynamic therapies. This paper reviews the research progress of CDs and their composites in targeted tumor imaging, detection, diagnosis, drug delivery and tumor killing. It also discusses and proposes the challenges and perspectives of their future applications in these fields. This review provides ideas for future applications of novel CD-based materials in the diagnosis and treatment of cancer.

Design of chemically defined synthetic substrate surfaces for the in vitro maintenance of human pluripotent stem cells: A review.

Human pluripotent stem cells (hPSCs) have the potential of long-term self-renewal and differentiation into nearly all cell types in vitro. Prior to the downstream applications, the design of chemically defined synthetic substrates for the large-scale proliferation of quality-controlled hPSCs is critical. Although great achievements have been made, Matrigel and recombinant proteins are still widely used in the fundamental research and clinical applications. Therefore, much effort is still needed to improve the performance of synthetic substrates in the culture of hPSCs, realizing their commercial applications. In this review, we summarized the design of reported synthetic substrates and especially their limitations in terms of cell culture. Moreover, much attention was paid to the development of promising peptide displaying surfaces. Besides, the biophysical regulation of synthetic substrate surfaces as well as the three-dimensional culture systems were described.