A 3-year experience of a simple, novel technique for accurate ostial/non-ostial coronary stenting: The buddy balloon anchor stent technique.

OBJECTIVE: To evaluate the safety and efficacy of a new technique for accurate ostial/non-ostial coronary stenting in percutaneous coronary intervention (PCI). BACKGROUND: Accurate stent localization is a key factor impacting the postoperative success of patients undergoing PCI. However, the accurate localization of some lesions, especially ostial lesions, is very difficult to achieve, because they are often complicated by bobbing or to-and-fro movement of the stent during cardiac contractions. METHODS: We report a novel technique of precise ostial/non-ostial stenting based on the buddy balloon anchor stent (BBAS) technique. Between May 2014 and July 2017, 47 patients with significant ostial/non-ostial coronary stenosis that required accurate stenting were included in this study. Of them, 23 patients were treated using the conventional method and the remaining 24 patients were treated using (BBAS) technique. Evaluation was then performed using intravascular ultrasound (IVUS) in the procedural, or coronary computed tomography angiography (CCTA) in the follow up. RESULTS: Using the BBAS technique, the procedural success was achieved in all 24 (100%) cases. IVUS was performed in seven patients (29.17%) and no procedural complications occurred. All six failed cases that occurred among patients with right coronary artery and left anterior descending artery ostial stenosis treated using the conventional method, the lesions were subsequently successfully re-stented using the BBAS technique. After a follow-up of 3-36 months, CCTA was performed in 11 patients (45.83%), all the stents were in the accurate position. There were no major cardiovascular events of death, myocardial infarction, or target lesion revascularization. CONCLUSION: BBAS is a simple, highly successful and safe technique for accurate stenting of difficult ostial/nonostial coronary stenosis lesions.

[Analysis of trace elements in liver, spleen and brain of rats infected with Toxoplasma gondii].

OBJECTIVE: To study the level of five trace elements(Fe2+, Cu2+, Zn2+, Ca2+, Mg2+) in the liver, spleen and brain of the rats infected with Toxoplasma gondii. METHODS: 20 rats were randomly and equally divided into two groups: normal group and experimental group. On the 64th day after injection of 1.5 x 10(6) T. gondii/2 ml, all of the rats were killed and the atomic absorption method were used. RESULTS: Compared with the normal group, the level of Cu2+ in liver in experimental group was significantly reduced (P < 0.01), and the amount of Fe2+ of sick rats were higher than that of the normal (P < 0.01). No differences in the content of Zn2+, Ca2+, Mg2+ between the experimental and control group were determined; the level of Fe2+, Cu2+ in spleen in experimental group was significantly altered (P < 0.01). The amount of Mg2+ of sick rats were higher than that of the normal (P < 0.01), and no difference on the content of Zn2+, Ca2+ between the experimental and control group were estimated; the level of Fe2+, Cu2+, Mg2+ in brain in experimental group was significantly reduced (P < 0.01). The amount of Ca2+ of sick rats were higher than that of the normal (P < 0.02). No difference on the content of Zn2+ between the experimental and control group were estimated. CONCLUSION: T. gondii infection might cause changes in trace elements in the liver, spleen and brain of rats.

[Determination of T lymphocytes and trace elements in spleen from rats infected with Toxoplasma gondii].

OBJECTIVE: To determine the level of five trace elements(Fe2+, Cu2+, Zn2+, Ca2+, Mg2+) in the spleen and changes of T lymphocyte and its subtype variations in peripheral blood from the rats infected with Toxoplasma gondii. METHODS: Twenty rats were randomly and equally divided into two groups: control group and experiment group. Each rat in the experiment group received an i.p. injection of 2 ml normal saline containing 1.5 x 10(6) tachyzoites of T. gondii. On the 64th day after injection of T. gondii, the changes in T lymphocytes (TL) and their subgroups, the helper T lymphocytes (Th) and the suppressor T lymphocytes(Ts) in the peripheral blood of the rats with T. gondii were determined by the assay of the lymphocytes labeled with intercellular acid alpha-naphthyl acetate esterase. All the rats were killed and the atomic absorption method were used for detecting the level of trace elements in the spleen tissue. RESULTS: The number of TL and Th in experiment group was significantly lower than that of control (P < 0.01, P < 0.01). The ratio of Th/Ts showed a significant difference between the two groups. The level of Fe2+, Cu2+ in experiment group was significantly reduced (P < 0.01). The amount of Mg2+ in infected rats was higher than that of the control(P < 0.01). No statistical difference in the content of Zn2+, Ca2+ was found between the two groups. CONCLUSION: T. gondii infection might cause the changes in the TL and Th in peripheral blood and the changes in trace elements in spleen of the rats.

[Detection of DNA of Toxoplasma gondii in rat by using polymerase chain reaction].

OBJECTIVE: To study the significance of DNA of Toxoplasma gondii in peripheral blood. METHODS: DNA of T. gondii in peripheral blood of 50 infected rats was detected by polymerase chain reaction. A pair of primers was designed, according to the sequence P30 gene specific to T. gondii, to amplify DNA from T. gondii by PCR. RESULTS: The primers amplified DNA specifically from T. gondii and could not amplify DNA from humans, uninfected rat and mouse and from Trichomonas vaginalis and Entamoeba histolytica. DNA of two Toxoplasma parasites was detected by 35 cycles of amplification, indicating a fair sensitivity of the PCR system. CONCLUSION: PCR may have a value for early diagnosis of T. gondii infection in rat.