RESUMEN
This study was designed to examine antioxidant activities, antiglycating abilities and neuroprotective effects of methanolic extracts of Salvia choloroleuca, Salvia santolinifolia and Salvia mirzayanii from Iran. The extracts were screened for their possible antioxidant activities by several biochemical assays such as DPPH, FRAP, ß-carotene bleaching and TEAC assays. HPLC analysis of these extracts led to the separation of a number of components such as catechine and rosmarinic acid. Based on our results, all these plants had antioxidant and antiglycating activities, among them S. choloroleuca seems to be the most effective one. Furthermore, these species not only showed no cytotoxic effects in neuron-like PC12 cells, but also protected them against oxidative stress-induced cell death, exerted by H(2)O(2). We further showed that these plants increase superoxide dismutase and catalase levels, reduce lipid peroxidation and up regulate hemeoxygenase-1 and glutamylcysteine synthetase proteins. This study raised the possibility of developing these plants as potential neuroprotective agents.
Asunto(s)
Antioxidantes/farmacología , Muerte Celular/efectos de los fármacos , Productos Finales de Glicación Avanzada/metabolismo , Fármacos Neuroprotectores/farmacología , Extractos Vegetales/farmacología , Salvia/química , Animales , Catalasa/metabolismo , Catequina/farmacología , Cinamatos/farmacología , Depsidos/farmacología , Glutamato-Cisteína Ligasa/metabolismo , Hemo-Oxigenasa 1/metabolismo , Peróxido de Hidrógeno , Irán , Peroxidación de Lípido/efectos de los fármacos , Neuronas/efectos de los fármacos , Células PC12 , Ratas , Superóxido Dismutasa/metabolismo , Regulación hacia Arriba , Ácido RosmarínicoRESUMEN
Increased oxidative stress is widely accepted to be a factor in the development and progression of Alzheimer's disease (AD). Here we introduced Salvia sahendica as a protective agent in differentiated PC12 cells, which are commonly considered to be a reliable model of neuronal cells. Our results demonstrated that S. sahendica has antioxidant and antiglycating properties in in vitro system and these properties are expanded into H(2)O(2)-induced model. S. sahendica inhibited H(2)O(2)-induced cell death in PC12 cells. We further showed that this plant exerts its protective effect by increasing superoxide dismutase and catalase levels, reducing lipid peroxidation and upregulating hemoxygenase-1 and glutamylcysteine synthetase proteins. This study raises the possibility of developing S. sahendica as a potential neuroprotective agent.