RESUMEN
A series of adenine-copper complexes (1-6) with various ligands (Cl(-), SCN(-), BF(4)(-) and acac [acetylacetonate ion]) have been synthesized and characterized by elemental analysis, infrared spectroscopy and thermal analysis. Among the six complexes only complex (1), Cu(2)(adenine)(4)Cl(4).2EtOH (abbreviated as Cu-Ad), demonstrated some toxic effect on different cell lines. In vitro investigations of the biological effect of Cu-Ad complex have shown that it: (1) binds genomic DNA; (2) decreases significantly, the viability of cells in culture in a concentration (15-125 microM)-dependant manner; an estimated IC(50) of: 45 microM with HepG2; 73 microM with C2C12; 103 microM with NIH3T3; and 108 microM with MCF7. Cu-Ad had no effect on A549 cells; (3) inhibits Taq polymerase-catalyzed reaction; (4) inhibits the binding of the transcription factor GATA-5 to labeled DNA probes; (5) inhibits mitochondrial NADH-UQ-reductase with an estimated IC(50) of 2.8 nmol, but had no effect on succinate dehydrogenase activity; (6) increases reactive oxygen species (60%) at 45 microM Cu-Ad; and (7) decreases ATP (80%) at 50 microM Cu-Ad. The new compound Cu(2)(adenine)(4)Cl(4).2EtOH (Cu-Ad), belongs to a class of copper-adenylate complexes that target many biochemical sites and with potential anti-cancer activity.
Asunto(s)
Adenina/análogos & derivados , Antineoplásicos/síntesis química , Cobre/farmacología , Compuestos Organometálicos/síntesis química , Compuestos Organometálicos/farmacología , Adenina/química , Adenina/farmacología , Adenosina Trifosfato/antagonistas & inhibidores , Adenosina Trifosfato/metabolismo , Antineoplásicos/química , Antineoplásicos/farmacología , Rastreo Diferencial de Calorimetría , Línea Celular Tumoral , Supervivencia Celular/efectos de los fármacos , Cobre/química , Complejo I de Transporte de Electrón/antagonistas & inhibidores , Complejo I de Transporte de Electrón/metabolismo , Ensayo de Cambio de Movilidad Electroforética , Factor de Transcripción GATA5/antagonistas & inhibidores , Factor de Transcripción GATA5/metabolismo , Humanos , Concentración 50 Inhibidora , Compuestos Organometálicos/química , Especies Reactivas de Oxígeno/metabolismo , Espectrofotometría Ultravioleta , Espectroscopía Infrarroja por Transformada de Fourier , Succinato Deshidrogenasa/antagonistas & inhibidores , Succinato Deshidrogenasa/metabolismo , Polimerasa Taq/antagonistas & inhibidores , Polimerasa Taq/metabolismo , TermogravimetríaRESUMEN
Sixteen Schiff bases obtained from the condensation of benzaldehyde or salicylaldehyde with various amines (aniline, 4-carboxyaniline, phenylhydrazine, 2,4-dinitrophenylhydrazine, ethylenediamine, hydrazine, o-phenylenediamine and 2,6-pyridinediamine) are studied with UV-vis spectroscopy to observe the effect of solvents, substituents and other structural factors on the spectra. The bands involving different electronic transitions are interpreted. Computerized analysis and multiple regression techniques were applied to calculate the regression and correlation coefficients based on the equation that relates peak position lambda(max) to the solvent parameters that depend on the H-bonding ability, refractive index and dielectric constant of solvents.
Asunto(s)
Iminas/química , Solventes/química , Electroquímica , Modelos Químicos , Análisis de Regresión , Bases de Schiff/química , Análisis Espectral , Estadística como AsuntoRESUMEN
Copper and cobalt complexes with 4-chloro-3-nitrobenzoate (ClNBz) and the nitrogen ligands 1,3-diaminopropane (1,3-DAP) or o-phenylenediamine (o-PDA), were prepared and characterized. The complexes [Cu(ClNBz)2(1,3-DAP)] (1), [Cu(ClNBz)(o-PDA)]Cl (2), [Co(ClNBz)2(1,3-DAP)] (3) and [Co(ClNBz)2(o-PDA)2] (4) were characterized by FTIR, UV-Visible absorption, elemental analysis and thermal analysis. Complex [Cu(ClNBz)(o-PDA)]Cl (2) shows high antibacterial activity as indicated by its ability to inhibit the growth of Staphylococcus aureus, Enterococcus faecalis.