RESUMEN
Spinocerebellar ataxia type 7 (SCA7) is an inherited neurodegenerative disorder characterized by progressive cerebellar ataxia associated with macular degeneration. We recently described one of the largest series of patients with SCA7 that originated from a founder effect in a Mexican population, which allowed us to perform herein the first comprehensive clinical, neurophysiological, and genetic characterization of Mexican patients with SCA7. In this study, 50 patients, categorized into adult or early phenotype, were clinically assessed using standard neurological exams and genotyped using fluorescent PCR and capillary electrophoresis. Patients with SCA7 exhibited the classical phenotype of the disease characterized by cerebellar ataxia and visual loss; however, we reported, for the first time, frontal-executive disorders and altered sensory-motor peripheral neuropathy in these patients. Semiquantitative analysis of ataxia-associated symptoms was performed using Scale for the Assessment and Rating of Ataxia (SARA) and the Brief Ataxia Rating Scale (BARS) scores, while extracerebellar features were measured employing the Inventory of Non-ataxia Symptoms (INAS) scale. Ataxia rating scales confirmed the critical role size of cytosine-adenine-guanine (CAG) repeat size on age at onset and disease severity, while analysis of CAG repeat instability showed that paternal rather than maternal transmission led to greater instability.
Asunto(s)
Ataxias Espinocerebelosas/diagnóstico , Ataxias Espinocerebelosas/genética , Adolescente , Adulto , Anciano , Anciano de 80 o más Años , Niño , Femenino , Genotipo , Humanos , Masculino , México , Persona de Mediana Edad , Pruebas Neuropsicológicas , Índice de Severidad de la Enfermedad , Ataxias Espinocerebelosas/fisiopatología , Ataxias Espinocerebelosas/psicología , Adulto JovenRESUMEN
Spinocerebellar ataxias (SCA) are a heterogeneous group of neurodegenerative disorders. CAG (cytosine-adenine-guanine) trinucleotide repeat expansions in the causative genes have been identified as the cause of different SCA. In this study, we simultaneously genotyped SCA1, SCA2, SCA3, SCA6, and SCA7 applying a fluorescent multiplex polymerase chain reaction assay. We analyzed 10 families with SCA (64 patients) from five different communities of Veracruz, a Mexican southeastern state, and identified 55 patients for SCA7 and 9 for SCA2, but none for SCA1, SCA3, or SCA6. To our knowledge, this sample represents one of the largest series of SCA7 cases reported worldwide. Genotyping of 300 healthy individuals from Mexican population and compiled data from different ethnicities showed discordant results concerning the hypothesis that SCA disease alleles arise by expansion of large normal alleles.
Asunto(s)
Efecto Fundador , Proteínas del Tejido Nervioso/genética , Ataxias Espinocerebelosas/epidemiología , Ataxias Espinocerebelosas/genética , Expansión de Repetición de Trinucleótido/genética , Ataxina-7 , Fluorescencia , Frecuencia de los Genes , Genotipo , Humanos , México/epidemiología , Reacción en Cadena de la Polimerasa Multiplex , PrevalenciaRESUMEN
Our aim was to study the specific role of the postsynaptic D(1) receptors on dopaminergic response and analyze the metabolized dopamine (DA) in the rat striatum. We used male Wistar rats to evaluate the effects of different doses of a D(1) agonist (SKF-38393) and a D(1) antagonist (SCH-23390), and their co-administration. The levels of DA and L-3, 4-dihydroxyphenylacetic acid (DOPAC) were measured using high performance liquid chromatography. The systemic injection of SKF-38393 alone at 1, 5 and 10 mg/kg did not alter the DA and DOPAC levels or the DOPAC/DA ratio. In contrast, injection of SCH-23390 alone at 0.25, 0.5 and 1 mg/kg significantly increased the DA and DOPAC levels, as well as the DOPAC/DA ratio, compared with the respective control groups. The co-administration of SCH-23390+SKF-38393 did not alter the DA or DOPAC levels, but it did significantly inhibit the SCH-23390-induced increase of the DA and DOPAC levels. The SCH-23390+SKF-38393 and the SCH-23390-only groups showed an increase in the DOPAC/DA ratio. The co-administration of SCH-23390+PARGYLINE significantly decreased the DOPAC levels and the DOPAC/DA ratio compared with the control and SCH-23390 groups. Taken together, our results showed that selective inhibition with SCH-23390 produced an increase in metabolized DA via striatal monoamine oxidase. These findings also contribute to the understanding of the role of postsynaptic D(1) receptors in the long-loop negative feedback system in the rat striatum.
Asunto(s)
Ácido 3,4-Dihidroxifenilacético/metabolismo , Cuerpo Estriado/efectos de los fármacos , Dopamina/metabolismo , Receptores de Dopamina D1/antagonistas & inhibidores , 2,3,4,5-Tetrahidro-7,8-dihidroxi-1-fenil-1H-3-benzazepina/administración & dosificación , 2,3,4,5-Tetrahidro-7,8-dihidroxi-1-fenil-1H-3-benzazepina/farmacología , Animales , Benzazepinas/administración & dosificación , Benzazepinas/farmacología , Cuerpo Estriado/metabolismo , Agonistas de Dopamina/farmacología , Antagonistas de Dopamina/farmacología , Masculino , Pargilina/administración & dosificación , Ratas , Ratas WistarRESUMEN
BACKGROUND: We describe two clinical cases and examine the effects of piracetam on the brainstem auditory response in infantile female rhesus monkeys (Macaca mulatta). RESULTS: We found that the interwave intervals show a greater reduction in a 3-year-old rhesus monkey compared to a 1-year-old rhesus monkey. DISCUSSION: In this report, we discuss the significance of these observations.
Asunto(s)
Potenciales Evocados Auditivos del Tronco Encefálico/efectos de los fármacos , Macaca mulatta , Nootrópicos/farmacología , Piracetam/farmacología , Anestesia , Animales , FemeninoRESUMEN
We studied the effects of high doses of pentobarbital (PB) and carbamazepine (CBZ) on electrolyte levels and pH in an epileptic animal model. Pentobarbital decreased Ca2+ and Na+ levels without pentylenetetrazole (PTZ). After this, Ca2+ and Na+ levels continued to decrease except when CBZ was used, which preserved the Ca2+ levels PTZ may have opposed effects on PB. Our results suggest that PB causes changes in electrolyte levels and pH, but these changes are diminished by CBZ.
Asunto(s)
Electrólitos/metabolismo , Concentración de Iones de Hidrógeno/efectos de los fármacos , Hipnóticos y Sedantes/farmacología , Pentobarbital/farmacología , Convulsiones/metabolismo , Animales , Anticonvulsivantes/farmacología , Anticonvulsivantes/uso terapéutico , Calcio/metabolismo , Carbamazepina/farmacología , Carbamazepina/uso terapéutico , Modelos Animales de Enfermedad , Interacciones Farmacológicas , Hipnóticos y Sedantes/uso terapéutico , Masculino , Pentobarbital/uso terapéutico , Pentilenotetrazol , Ratas , Ratas Wistar , Convulsiones/inducido químicamente , Convulsiones/tratamiento farmacológico , Sodio/metabolismo , Estadísticas no ParamétricasRESUMEN
It has been discussed that serotonin (5-HT) could be involved in the effects of sleep deprivation (SD) and/or malnutrition (M) on the sleep-wake cycle. The aim of this work was to study the effects of the M, SD and its interaction on 5-HT and 5-hydroxy-indole-acetic acid (5-HIAA) contents in the dorsal raphe (DR) and the suprachiasmatic nuclei (SCN), two sleep-wake cycle regulators. Forty-eight puppets rats were obtained from mothers fed with low or normal casein diet. They were allocated in 3 groups (n=16 each): prenatal/postnatal casein malnutrition (6/6%), prenatal casein malnutrition/nutritional casein rehabilitation (6/25%) and prenatal/postnatal casein well-nourished state (25/25%). When rats were 60 days old, 24 animals were exposed to sleep deprivation by means of forced locomotion during 24 h. The remaining 24 were kept under normal conditions of sleep-wake cycle. Then, all animals were sacrificed by decapitation. DR and SCN were dissected and processed to determine the 5-HT and 5-HIAA contents by means of HPLC. It was observed that 6/6% rats showed a 5-HT increase (DR p<0.011; SCN p<0.019) as well as in SD (DR p<0.0008; SCN p<0.0009) with respect to 25/25% rats. No differences were found in 6/25% rats. Therefore, 5-HIAA decreased significantly in both nuclei in all the groups, notably in M+SD animals (DR p<0.001; SCN p<0.001). We conclude that the sleep-wake cycle disruptions produced by chronic M and SD are mediated in part by a synergistic effect on 5-HT in the DR-SCN pathway, perhaps due to a delay in the development of such brain structures.
Asunto(s)
Ácido Hidroxiindolacético/metabolismo , Desnutrición , Núcleos del Rafe/metabolismo , Serotonina/metabolismo , Privación de Sueño/metabolismo , Núcleo Supraquiasmático/metabolismo , Animales , Conducta Animal , Cromatografía Líquida de Alta Presión/métodos , Masculino , Desnutrición/metabolismo , Desnutrición/patología , Desnutrición/rehabilitación , Ratas , Ratas Sprague-DawleyRESUMEN
Sleep alterations and brain regional changes of serotonin were studied in rats exposed to 1.5 ppm of ozone (O3). Results showed a significant decrease in the time spent in wakefulness (W) and paradoxical sleep (PS) and a significant increase in the time spent in slow wave sleep (SWS). Neurochemical analysis showed a significant increase in the metabolism of serotonin in medulla oblongata, pons and midbrain, while both serotonin and its metabolite were reduced in hypothalamus. Although other neurotransmitters could be affected by O3 exposure, the sleep disorders observed in the present work may be related to alterations in the metabolism of serotonin produced by the exposure to O3.
Asunto(s)
Química Encefálica/efectos de los fármacos , Ozono/toxicidad , Serotonina/metabolismo , Fases del Sueño/efectos de los fármacos , Trastornos del Sueño-Vigilia/inducido químicamente , Animales , Cromatografía Líquida de Alta Presión , Ácido Hidroxiindolacético/análisis , Masculino , Especificidad de Órganos , Ozono/farmacología , Polisomnografía , Ratas , Ratas Wistar , Trastornos del Sueño-Vigilia/metabolismo , Vigilia/efectos de los fármacosRESUMEN
INTRODUCTION: The basal ganglia include the striatum, globus pallidus, the substantia nigra pars compacta and pars reticulata. The striatum receives afferent input from the substantia nigra pars compacta. The principal neurons of the striatum are medium spiny neurons, that express high levels of D1 and D2 receptors. AIMS: This review deals about the aspects underlying to the negative feedback via long-loop in the striatal dopamine release modulation in the rat. Also, the motor function in dopamine receptor knock-out mice is discussed. DEVELOPMENT AND CONCLUSIONS: The intrastriatal infusion and systemic injection of dopamine receptor agonists and antagonists may regulate the striatal dopamine release and induce changes in motor function. Disruption of the D1 and D2 gene shown that the motor function is controlled by D1 and D2 receptors. The study of the long-loop negative feedback may contribute to our understanding in the physiology and dysfunction of basal ganglia.
Asunto(s)
Cuerpo Estriado/metabolismo , Dopamina/metabolismo , Retroalimentación Fisiológica/fisiología , Vías Nerviosas/fisiología , Animales , Cuerpo Estriado/citología , Agonistas de Dopamina/metabolismo , Antagonistas de Dopamina/metabolismo , Globo Pálido/citología , Globo Pálido/metabolismo , Actividad Motora/fisiología , Vías Nerviosas/anatomía & histología , Neuronas/citología , Neuronas/metabolismo , Ratas , Receptores de Dopamina D1/metabolismo , Receptores de Dopamina D2/metabolismo , Sustancia Negra/citología , Sustancia Negra/metabolismoRESUMEN
INTRODUCTION: Otoacoustic emissions are evidence of the existence of an active process in the cochlea, and the motility of the outer hair cells means that they can change the cochlear mechanical response. We believe that incorrect processing of the sounds of language in the cochlea can result in impaired language processes. SUBJECTS AND METHODS: Data were collected from the patient record; neurological, visual and auditory examination; Weschler intelligence scale; initial language test (ILT); brainstem auditory evoked potentials (BAEPs) and transient otoacoustic emissions. The results of the ILT were used to form three groups: controls, pathological and pathological with normal ILT. RESULTS: All the children presented a response at 20 dB by means of the BAEPs. In the transient otoacoustic emissions, Student's t test was conducted between the right and the left ear for total reproducibility and reproducibility at different band frequencies within each group. No significant differences were observed. The same test was carried out between groups (controls versus pathological, controls versus pathological with normal ILT, and pathological versus pathological with normal ILT) in the right and left ears; no significant differences were found in the total reproducibility for the two ears. No significant differences were found in the reproducibility at different frequency bands for the left ear, but some were found in the case of the right ear. CONCLUSIONS: Laterality from the periphery exists for language processing and if this process fails to perform correctly, due to malfunctioning of the outer hair cells, language may be affected.
Asunto(s)
Cóclea/fisiopatología , Trastornos del Lenguaje/etiología , Niño , Preescolar , Células Ciliadas Auditivas Externas/fisiología , Pruebas Auditivas , Humanos , MasculinoRESUMEN
AIM: A brief revision was performed in order to develop a topographic model of cerebral activation during silent reading, syllable's repetition and emotional prosody, based in recent neuroimaging studies. DEVELOPMENT: It has been reported that the words are analyzed in both occipital hemispheres during silent reading, after they are 'written' in the right temporal cortex while the integration of the semantic and phonologic processes are integrated on different left temporal areas and also in the left frontal lower part. The understanding is achieved in the left-middle temporal cortex. In the other hand, activation during articulatory movements is carried out in the left supratemporal gyrus and the left motor and premotor cortex, the left putamen and part of both hemispheres of the cerebellum. Finally, recognition of the emotional prosody occurs in three stages: obtaining of the acoustic information in some areas of the right temporal lobe, representation of acoustic sequences in the right posterio-superior temporal area, and an evaluation of the emotional prosody in the lower frontal bilateral cortex, with the involvement of the basal ganglia in the emotional expression. CONCLUSIONS: The location and synchrony of the areas that activate during the language processing is lateralized toward the left hemisphere and it involves cortical and subcortical areas, except for the emotional prosody. The understanding of the language processes will open the field for to take advantage of the plastic mechanisms for the speech therapy and rehabilitation.
Asunto(s)
Corteza Cerebral/anatomía & histología , Lenguaje , Mapeo Encefálico , Corteza Cerebral/fisiología , Emociones , Lateralidad Funcional/fisiología , Humanos , Magnetoencefalografía , Lectura , Percepción del HablaRESUMEN
INTRODUCTION: The cortical ablation has been used as an experimental model in order to study the basic mechanisms of functional recovery. However, there is not data concerning to the injury effects on the motor and somatosensorial behavioral manifestations that allow us to categorize such sequels as a hemiplegic model. MATERIALS AND METHODS: We used 35 male Wistar rats (280-300 g) allocated in two groups: control (n = 17) and brain injured by cortical ablation (n = 18). Previously trained, basal recordings of the footprint and motor and somatosensorial assessment were performed in the rats before surgery. The behavioral tests were performed again 6 hours after surgery and the spontaneous ambulatory activity was also evaluated. RESULTS AND CONCLUSIONS: It was observed a decrease in the stride's length and an increase in the stride's angle and in the motor deficit, while the somatosensorial assessment and spontaneous ambulatory activity were not affected. These findings are discussed in function of the motor features of the hemiparetic sequels in humans.
Asunto(s)
Conducta Animal/fisiología , Corteza Cerebral/patología , Actividad Motora/fisiología , Animales , Lesiones Encefálicas/fisiopatología , Corteza Cerebral/fisiología , Masculino , Desempeño Psicomotor , Ratas , Ratas Wistar , Recuperación de la FunciónRESUMEN
We have shown in our laboratory that cat's and rat's sleep disturbances are produced by 24 h of ozone (O3) exposure, indicating that the central nervous system is affected by this gas. To demonstrate the probable changes in brain neurotransmitters, we evaluated the monoamine contents of the midbrain and striatum of rats exposed to 1 part per million O3 for 1 or 3 hours periods. The results were compared with rats exposed to fresh air and to those exposed to 3 hours of O3 followed by 1 or 3 hours of fresh air. We found a significant increase in dopamine (DA) and its metabolites noradrenaline (NA) and 3,4 dihydroxyphenylacetic acid (DOPAC), as well as an increase in the 5-hydroxyindolacetic acid (5-HIAA) contents of the striatum. There were no changes in homovanillic acid (HVA) and serotonin (5-HT) levels during O3 exposure. Additionally, an increase in DA, NA and 5-HIAA in the midbrain during O3 exposure was observed. Turnover analysis revealed that DA increased more than its metabolites in both the midbrain and striatum. However, the metabolite of 5-HT, i.e. 5-HIAA, increased more than its precursor, this reaching statistical significance only in the midbrain. These findings demonstrate that O3 or its reaction products affect the metabolism of major neurotransmitter systems as rapidly as after 1 h of exposition.