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1.
Proc Natl Acad Sci U S A ; 115(15): 3936-3941, 2018 04 10.
Artículo en Inglés | MEDLINE | ID: mdl-29581279

RESUMEN

Bietti's crystalline dystrophy (BCD) is an intractable and progressive chorioretinal degenerative disease caused by mutations in the CYP4V2 gene, resulting in blindness in most patients. Although we and others have shown that retinal pigment epithelium (RPE) cells are primarily impaired in patients with BCD, the underlying mechanisms of RPE cell damage are still unclear because we lack access to appropriate disease models and to lesion-affected cells from patients with BCD. Here, we generated human RPE cells from induced pluripotent stem cells (iPSCs) derived from patients with BCD carrying a CYP4V2 mutation and successfully established an in vitro model of BCD, i.e., BCD patient-specific iPSC-RPE cells. In this model, RPE cells showed degenerative changes of vacuolated cytoplasm similar to those in postmortem specimens from patients with BCD. BCD iPSC-RPE cells exhibited lysosomal dysfunction and impairment of autophagy flux, followed by cell death. Lipidomic analyses revealed the accumulation of glucosylceramide and free cholesterol in BCD-affected cells. Notably, we found that reducing free cholesterol by cyclodextrins or δ-tocopherol in RPE cells rescued BCD phenotypes, whereas glucosylceramide reduction did not affect the BCD phenotype. Our data provide evidence that reducing intracellular free cholesterol may have therapeutic efficacy in patients with BCD.


Asunto(s)
Colesterol/metabolismo , Distrofias Hereditarias de la Córnea/metabolismo , Enfermedades de la Retina/metabolismo , Animales , Colesterol/análisis , Distrofias Hereditarias de la Córnea/dietoterapia , Distrofias Hereditarias de la Córnea/enzimología , Distrofias Hereditarias de la Córnea/genética , Familia 4 del Citocromo P450/genética , Familia 4 del Citocromo P450/metabolismo , Humanos , Ratones , Mutación , Fenotipo , Enfermedades de la Retina/dietoterapia , Enfermedades de la Retina/enzimología , Enfermedades de la Retina/genética , Epitelio Pigmentado de la Retina/enzimología , Epitelio Pigmentado de la Retina/metabolismo
2.
Hum Mol Genet ; 24(13): 3775-91, 2015 Jul 01.
Artículo en Inglés | MEDLINE | ID: mdl-25859007

RESUMEN

Distinct mutations in the centrosomal-cilia protein CEP290 lead to diverse clinical findings in syndromic ciliopathies. We show that CEP290 localizes to the transition zone in ciliated cells, precisely to the region of Y-linkers between central microtubules and plasma membrane. To create models of CEP290-associated ciliopathy syndromes, we generated Cep290(ko/ko) and Cep290(gt/gt) mice that produce no or a truncated CEP290 protein, respectively. Cep290(ko/ko) mice exhibit early vision loss and die from hydrocephalus. Retinal photoreceptors in Cep290(ko/ko) mice lack connecting cilia, and ciliated ventricular ependyma fails to mature. The minority of Cep290(ko/ko) mice that escape hydrocephalus demonstrate progressive kidney pathology. Cep290(gt/gt) mice die at mid-gestation, and the occasional Cep290(gt/gt) mouse that survives shows hydrocephalus and severely cystic kidneys. Partial loss of CEP290-interacting ciliopathy protein MKKS mitigates lethality and renal pathology in Cep290(gt/gt) mice. Our studies demonstrate domain-specific functions of CEP290 and provide novel therapeutic paradigms for ciliopathies.


Asunto(s)
Cilios/metabolismo , Hidrocefalia/genética , Enfermedades Renales Quísticas/genética , Proteínas Nucleares/genética , Animales , Antígenos de Neoplasias , Proteínas de Ciclo Celular , Cilios/genética , Proteínas del Citoesqueleto , Modelos Animales de Enfermedad , Femenino , Humanos , Hidrocefalia/metabolismo , Enfermedades Renales Quísticas/metabolismo , Masculino , Ratones , Ratones Endogámicos C57BL , Ratones Noqueados , Proteínas Nucleares/metabolismo , Especificidad de Órganos
3.
Retina ; 37(6): 1193-1202, 2017 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-27658286

RESUMEN

PURPOSE: To compare atrophy of the choroid and retina between Bietti crystalline dystrophy (BCD) patients and EYS-related retinitis pigmentosa (RP) patients with a similar degree of central visual field defects, age, and axial length (AL). METHODS: Nine eyes of nine BCD patients with CYP4V2 mutations (BCD group) were examined. Moreover, we selected 10 eyes of 10 RP patients with EYS mutations matched for age, axial length, and mean deviation (measured with the 10-2 SITA standard program; EYS-RP group), and 10 eyes of 10 normal volunteers matched for age and axial length (control group). Macular thicknesses of the choroid and retina were measured via swept-source optical coherence tomography. RESULTS: The macular choroid was significantly thinner in the BCD group than in the EYS-RP and control groups, although the thickness did not significantly differ between the EYS-RP and control groups. The macular retina was significantly thinner in the BCD and EYS-RP groups than in the control group, although the thickness did not significantly differ between the BCD and EYS-RP groups at most sites. CONCLUSION: Bietti crystalline dystrophy patients with CYP4V2 mutations showed more severe macular choroid atrophy as compared to EYS-related RP patients. These different damage patterns suggest differences in choroidal expression between CYP4V2 and EYS.


Asunto(s)
Coroides/patología , Distrofias Hereditarias de la Córnea/genética , Familia 4 del Citocromo P450/genética , Proteínas del Ojo/genética , Mácula Lútea/patología , Mutación , Enfermedades de la Retina/genética , Retinitis Pigmentosa/genética , Anomalías Múltiples , Adulto , Anciano , Atrofia/patología , Lámina Basal de la Coroides/patología , Distrofias Hereditarias de la Córnea/diagnóstico , Familia 4 del Citocromo P450/metabolismo , ADN/genética , Análisis Mutacional de ADN , Proteínas del Ojo/metabolismo , Femenino , Humanos , Masculino , Persona de Mediana Edad , Enfermedades de la Retina/diagnóstico , Retinitis Pigmentosa/diagnóstico , Tomografía de Coherencia Óptica
4.
Mol Vis ; 22: 150-60, 2016.
Artículo en Inglés | MEDLINE | ID: mdl-26957898

RESUMEN

PURPOSE: To investigate the efficacy of targeted exome sequencing for mutational screening of Japanese patients with cone dystrophy (CD) or cone-rod dystrophy (CRD). METHODS: DNA samples from 43 Japanese patients with CD or CRD were sequenced using an exome-sequencing panel targeting all 193 known inherited eye disease genes and next-generation sequencing methodologies. Subsequently, candidate variants were screened using systematic data analyses, and their potential pathogenicity was assessed using distinct filtering approaches, which included the frequency of the variants in normal populations, in silico prediction tools, and cosegregation. RESULTS: Causative mutations were detected in 12 patients with CD or CRD (27.9%). In total, 14 distinct mutations were identified in the genes ABCA4, CDHR1, CRB1, CRX, GUCY2D, KCNV2, PROM1, PRPH2, and RDH5, including four novel mutations, c.3050+1G>A in ABCA4, c.386A>G in CDHR1, c.652+1_652+4del in CRB1, and c.454G>A in KCNV2. Moreover, a putative pathogenic mutation was identified in RGS9BP, a gene recognized as the source of bradyopsia. CONCLUSIONS: Targeted exome sequencing effectively identified causative mutations in Japanese patients with CD or CRD. The results confirmed the heterogeneity of the genes responsible for CD and CRD in Japanese populations, as well as the efficacy of targeted exome sequencing-based screening of patients with inherited retinal degeneration.


Asunto(s)
Proteínas del Ojo/genética , Mutación , Retinitis Pigmentosa/genética , Pueblo Asiatico/genética , Análisis Mutacional de ADN , Exoma/genética , Femenino , Secuenciación de Nucleótidos de Alto Rendimiento , Humanos , Japón/epidemiología , Masculino , Técnicas de Diagnóstico Molecular , Linaje , Retinitis Pigmentosa/diagnóstico , Tomografía de Coherencia Óptica , Adulto Joven
5.
Adv Exp Med Biol ; 854: 299-305, 2016.
Artículo en Inglés | MEDLINE | ID: mdl-26427425

RESUMEN

To examine the similarity of wide-field fundus autofluorescence (FAF) imaging in inherited retinal dystrophy between siblings and between parents and their children. The subjects included 17 siblings (12 with retinitis pigmentosa and 5 with cone rod dystrophy) and 10 parent-child pairs (8 with retinitis pigmentosa and 2 with cone rod dystrophy). We quantified the similarity of wide-field FAF using image processing techniques of cropping, binarization, superimposition, and subtraction. The estimated similarity of the siblings was compared with that of the parent-child pairs and that of the age-matched unrelated patients. The similarity between siblings was significantly higher that of parent-child pairs or that of age-matched unrelated patients (P = 0.004 and P = 0.049, respectively). Wide-field FAF images were similar between siblings with inherited retinal dystrophy but different between parent-child pairs. This suggests that aging is a confounding factor in genotype-phenotype correlation studies.


Asunto(s)
Fluorescencia , Fondo de Ojo , Lipofuscina/química , Distrofias Retinianas/diagnóstico , Factores de Edad , Salud de la Familia , Estudios de Asociación Genética , Genotipo , Humanos , Lipofuscina/metabolismo , Microscopía Confocal , Oftalmoscopía , Fenotipo , Distrofias Retinianas/genética , Distrofias Retinianas/metabolismo , Epitelio Pigmentado de la Retina/química , Epitelio Pigmentado de la Retina/metabolismo , Epitelio Pigmentado de la Retina/patología , Retinitis Pigmentosa/diagnóstico , Retinitis Pigmentosa/genética , Retinitis Pigmentosa/metabolismo , Hermanos , Tomografía de Coherencia Óptica
6.
Ophthalmology ; 122(11): 2295-2302.e2, 2015 Nov.
Artículo en Inglés | MEDLINE | ID: mdl-26337002

RESUMEN

PURPOSE: Age-related macular degeneration (AMD) is the leading cause of severe visual impairment. Despite treatment, a central scotoma often remains. The size of the scotoma depends on the lesion size of the choroidal neovascular membrane and significantly affects the patient's quality of life, and the lesion size of neovascularization also affects response to treatments. The aim of this study was to identify genes associated with the neovascular lesion size in neovascular AMD. DESIGN: A genome-wide association study (GWAS). PARTICIPANTS: We included 1146 Japanese patients with neovascular AMD. METHODS: We performed a 2-stage GWAS for the lesion size of AMD as a quantitative trait among 1146 (first stage: 727, second stage: 419) Japanese patients with neovascular AMD. Lesion size was determined by the greatest linear dimension measured with fluorescein angiography examination before treatment. We examined the association between the genotypic distribution of each single nucleotide polymorphism (SNP) and the trait using an additive model adjusted for age and sex. To evaluate the associations between AMD development and SNPs associated with lesion size, we also performed a case-control study by using the genotype data from these 1146 Japanese patients as case subjects and the fixed dataset from the Nagahama Study as control subjects. MAIN OUTCOME MEASURES: Genes associated with the lesion size in neovascular AMD. RESULTS: In the discovery stage, rs10895322 in MMP20 showed a genome-wide significant P value of 6.95×10(-8), and rs2284665 in ARMS2/HTRA1 showed a P value of 1.55×10(-7). The associations of these 2 SNPs were successfully replicated in the replication stage, and a meta-analysis of both stages showed genome-wide significant P values (2.80×10(-9) and 4.41×10(-9), respectively). In a case-control study using 3248 Japanese subjects as controls, we could not find contribution of MMP20 rs10895322 for AMD development. Although MMP20 has been thought to be expressed only in dental tissues, we confirmed MMP20 expression in the human retina and retinal pigment epithelium/choroid with polymerase chain reaction. CONCLUSIONS: The growth of choroidal neovascularization in AMD would be affected by 2 genes: MMP20, a newly confirmed gene expressed in the retina, and ARMS2/HTRA1, a well-known susceptibility gene for AMD.


Asunto(s)
Metaloproteinasa 20 de la Matriz/genética , Polimorfismo de Nucleótido Simple , Proteínas/genética , Serina Endopeptidasas/genética , Degeneración Macular Húmeda/genética , Anciano , Anciano de 80 o más Años , Pueblo Asiatico/etnología , Estudios de Casos y Controles , Femenino , Angiografía con Fluoresceína , Predisposición Genética a la Enfermedad , Estudio de Asociación del Genoma Completo , Genotipo , Serina Peptidasa A1 que Requiere Temperaturas Altas , Humanos , Japón/epidemiología , Masculino , Reacción en Cadena de la Polimerasa , Escotoma/genética , Escotoma/patología , Degeneración Macular Húmeda/patología
7.
PLoS Genet ; 8(4): e1002649, 2012.
Artículo en Inglés | MEDLINE | ID: mdl-22511886

RESUMEN

A stringent control of homeostasis is critical for functional maintenance and survival of neurons. In the mammalian retina, the basic motif leucine zipper transcription factor NRL determines rod versus cone photoreceptor cell fate and activates the expression of many rod-specific genes. Here, we report an integrated analysis of NRL-centered gene regulatory network by coupling chromatin immunoprecipitation followed by high-throughput sequencing (ChIP-Seq) data from Illumina and ABI platforms with global expression profiling and in vivo knockdown studies. We identified approximately 300 direct NRL target genes. Of these, 22 NRL targets are associated with human retinal dystrophies, whereas 95 mapped to regions of as yet uncloned retinal disease loci. In silico analysis of NRL ChIP-Seq peak sequences revealed an enrichment of distinct sets of transcription factor binding sites. Specifically, we discovered that genes involved in photoreceptor function include binding sites for both NRL and homeodomain protein CRX. Evaluation of 26 ChIP-Seq regions validated their enhancer functions in reporter assays. In vivo knockdown of 16 NRL target genes resulted in death or abnormal morphology of rod photoreceptors, suggesting their importance in maintaining retinal function. We also identified histone demethylase Kdm5b as a novel secondary node in NRL transcriptional hierarchy. Exon array analysis of flow-sorted photoreceptors in which Kdm5b was knocked down by shRNA indicated its role in regulating rod-expressed genes. Our studies identify candidate genes for retinal dystrophies, define cis-regulatory module(s) for photoreceptor-expressed genes and provide a framework for decoding transcriptional regulatory networks that dictate rod homeostasis.


Asunto(s)
Factores de Transcripción con Cremalleras de Leucina de Carácter Básico/genética , Factores de Transcripción con Cremalleras de Leucina de Carácter Básico/metabolismo , Proteínas del Ojo/genética , Proteínas del Ojo/metabolismo , Proteínas de Homeodominio , Homeostasis , Retina , Distrofias Retinianas , Células Fotorreceptoras Retinianas Bastones/metabolismo , Transactivadores , Animales , Sitios de Unión , Proteínas de Unión al ADN/genética , Proteínas de Unión al ADN/metabolismo , Modelos Animales de Enfermedad , Técnicas de Silenciamiento del Gen , Redes Reguladoras de Genes , Secuenciación de Nucleótidos de Alto Rendimiento , Proteínas de Homeodominio/genética , Proteínas de Homeodominio/metabolismo , Homeostasis/genética , Humanos , Histona Demetilasas con Dominio de Jumonji/genética , Histona Demetilasas con Dominio de Jumonji/metabolismo , Ratones , Ratones Endogámicos C57BL , Neuronas/metabolismo , Retina/metabolismo , Retina/fisiología , Distrofias Retinianas/genética , Distrofias Retinianas/metabolismo , Transactivadores/genética , Transactivadores/metabolismo , Factores de Transcripción/genética , Factores de Transcripción/metabolismo , Transcripción Genética
8.
Stem Cells ; 31(6): 1149-59, 2013 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-23495178

RESUMEN

Replacement of dysfunctional or dying photoreceptors offers a promising approach for retinal neurodegenerative diseases, including age-related macular degeneration and retinitis pigmentosa. Several studies have demonstrated the integration and differentiation of developing rod photoreceptors when transplanted in wild-type or degenerating retina; however, the physiology and function of the donor cells are not adequately defined. Here, we describe the physiological properties of developing rod photoreceptors that are tagged with green fluorescent protein (GFP) driven by the promoter of rod differentiation factor, Nrl. GFP-tagged developing rods show Ca(2 +) responses and rectifier outward currents that are smaller than those observed in fully developed photoreceptors, suggesting their immature developmental state. These immature rods also exhibit hyperpolarization-activated current (Ih ) induced by the activation of hyperpolarization-activated cyclic nucleotide-gated (HCN) channels. When transplanted into the subretinal space of wild-type or retinal degeneration mice, GFP-tagged developing rods can integrate into the photoreceptor outer nuclear layer in wild-type mouse retina and exhibit Ca(2 +) responses and membrane current comparable to native rod photoreceptors. A proportion of grafted rods develop rhodopsin-positive outer segment-like structures within 2 weeks after transplantation into the retina of Crx-knockout mice and produce rectifier outward current and Ih upon membrane depolarization and hyperpolarization. GFP-positive rods derived from induced pluripotent stem (iPS) cells also display similar membrane current Ih as native developing rod photoreceptors, express rod-specific phototransduction genes, and HCN-1 channels. We conclude that Nrl-promoter-driven GFP-tagged donor photoreceptors exhibit physiological characteristics of rods and that iPS cell-derived rods in vitro may provide a renewable source for cell-replacement therapy.


Asunto(s)
Proteínas de Homeodominio/genética , Células Fotorreceptoras de Vertebrados/fisiología , Retina/fisiología , Degeneración Retiniana/terapia , Células Fotorreceptoras Retinianas Bastones/fisiología , Células Fotorreceptoras Retinianas Bastones/trasplante , Transactivadores/genética , Animales , Factores de Transcripción con Cremalleras de Leucina de Carácter Básico/genética , Factores de Transcripción con Cremalleras de Leucina de Carácter Básico/metabolismo , Calcio/metabolismo , Diferenciación Celular/genética , Células Cultivadas , Proteínas del Ojo/genética , Proteínas del Ojo/metabolismo , Proteínas Fluorescentes Verdes/genética , Proteínas Fluorescentes Verdes/metabolismo , Proteínas de Homeodominio/metabolismo , Canales Regulados por Nucleótidos Cíclicos Activados por Hiperpolarización/genética , Canales Regulados por Nucleótidos Cíclicos Activados por Hiperpolarización/metabolismo , Células Madre Pluripotentes Inducidas/metabolismo , Células Madre Pluripotentes Inducidas/fisiología , Potenciales de la Membrana/genética , Ratones , Ratones Endogámicos C57BL , Ratones Noqueados , Células Fotorreceptoras de Vertebrados/metabolismo , Regiones Promotoras Genéticas , Retina/metabolismo , Degeneración Retiniana/genética , Degeneración Retiniana/metabolismo , Degeneración Retiniana/fisiopatología , Células Fotorreceptoras Retinianas Bastones/metabolismo , Trasplante de Células Madre , Transactivadores/metabolismo
9.
Graefes Arch Clin Exp Ophthalmol ; 252(7): 1065-9, 2014 Jul.
Artículo en Inglés | MEDLINE | ID: mdl-24441883

RESUMEN

PURPOSE: To investigate the relationship between impairment of cone/rod photoreceptors and changes in optical coherence tomography (OCT) findings. METHODS: We retrospectively reviewed the clinical records of 35 patients with cone-rod dystrophy (CRD) and 35 visual acuity-matched patients with retinitis pigmentosa (RP). The presence or absence of the external limiting membrane (ELM), inner segment ellipsoid (ISe), interdigitation zone (IZ), and foveal cavitation (hyporeflective space in the outer retina) were determined using OCT image evaluation. RESULTS: There were no statistical differences in the number of CRD and RP patients with an intact ELM and ISe. None of the CRD patients had an intact IZ, but 20 % of RP patients did (P = 0.011). In addition, foveal cavitation tended to be observed more frequently in CRD patients than (25.7 %) in RP patients (5.7 %) despite the difference not being significant after the correction of multiple comparison. CONCLUSIONS: Eyes with CRD and RP had significant differences in foveal morphology, even when visual acuity was matched. This result supports the notion that absence of an IZ and the presence of foveal cavitation is related to cone-dominant photoreceptor impairment.


Asunto(s)
Células Fotorreceptoras de Vertebrados/patología , Segmento Interno de las Células Fotorreceptoras Retinianas/patología , Segmento Externo de las Células Fotorreceptoras Retinianas/patología , Retinitis Pigmentosa/diagnóstico , Tomografía de Coherencia Óptica , Anciano , Membrana Basal/patología , Electrorretinografía , Femenino , Humanos , Masculino , Persona de Mediana Edad , Estudios Retrospectivos , Agudeza Visual/fisiología , Campos Visuales/fisiología
10.
J Neurosci ; 32(2): 528-41, 2012 Jan 11.
Artículo en Inglés | MEDLINE | ID: mdl-22238088

RESUMEN

Cone photoreceptors are the primary initiator of visual transduction in the human retina. Dysfunction or death of rod photoreceptors precedes cone loss in many retinal and macular degenerative diseases, suggesting a rod-dependent trophic support for cone survival. Rod differentiation and homeostasis are dependent on the basic motif leucine zipper transcription factor neural retina leucine zipper (NRL). The loss of Nrl (Nrl(-/-)) in mice results in a retina with predominantly S-opsin-containing cones that exhibit molecular and functional characteristics of wild-type cones. Here, we report that Nrl(-/-) retina undergoes a rapid but transient period of degeneration in early adulthood, with cone apoptosis, retinal detachment, alterations in retinal vessel structure, and activation and translocation of retinal microglia. However, cone degeneration stabilizes by 4 months of age, resulting in a thinner but intact outer nuclear layer with residual cones expressing S- and M-opsins and a preserved photopic electroretinogram. At this stage, microglia translocate back to the inner retina and reacquire a quiescent morphology. Gene profiling analysis during the period of transient degeneration reveals misregulation of genes related to stress response and inflammation, implying their involvement in cone death. The Nrl(-/-) mouse illustrates the long-term viability of cones in the absence of rods and retinal pigment epithelium defects in a rodless retina. We propose that Nrl(-/-) retina may serve as a model for elucidating mechanisms of cone homeostasis and degeneration that would be relevant to understanding diseases of the cone-dominant human macula.


Asunto(s)
Apoptosis/genética , Factores de Transcripción con Cremalleras de Leucina de Carácter Básico/genética , Proteínas del Ojo/genética , Retina/anomalías , Retina/metabolismo , Células Fotorreceptoras Retinianas Conos/metabolismo , Degeneración Retiniana/fisiopatología , Animales , Factores de Transcripción con Cremalleras de Leucina de Carácter Básico/deficiencia , Modelos Animales de Enfermedad , Femenino , Masculino , Ratones , Ratones Endogámicos C57BL , Ratones Noqueados , Células Fotorreceptoras Retinianas Conos/patología , Degeneración Retiniana/genética , Degeneración Retiniana/patología , Desprendimiento de Retina/genética , Desprendimiento de Retina/patología , Desprendimiento de Retina/fisiopatología
11.
Proc Natl Acad Sci U S A ; 107(16): 7401-6, 2010 Apr 20.
Artículo en Inglés | MEDLINE | ID: mdl-20385819

RESUMEN

We executed a genome-wide association scan for age-related macular degeneration (AMD) in 2,157 cases and 1,150 controls. Our results validate AMD susceptibility loci near CFH (P < 10(-75)), ARMS2 (P < 10(-59)), C2/CFB (P < 10(-20)), C3 (P < 10(-9)), and CFI (P < 10(-6)). We compared our top findings with the Tufts/Massachusetts General Hospital genome-wide association study of advanced AMD (821 cases, 1,709 controls) and genotyped 30 promising markers in additional individuals (up to 7,749 cases and 4,625 controls). With these data, we identified a susceptibility locus near TIMP3 (overall P = 1.1 x 10(-11)), a metalloproteinase involved in degradation of the extracellular matrix and previously implicated in early-onset maculopathy. In addition, our data revealed strong association signals with alleles at two loci (LIPC, P = 1.3 x 10(-7); CETP, P = 7.4 x 10(-7)) that were previously associated with high-density lipoprotein cholesterol (HDL-c) levels in blood. Consistent with the hypothesis that HDL metabolism is associated with AMD pathogenesis, we also observed association with AMD of HDL-c-associated alleles near LPL (P = 3.0 x 10(-3)) and ABCA1 (P = 5.6 x 10(-4)). Multilocus analysis including all susceptibility loci showed that 329 of 331 individuals (99%) with the highest-risk genotypes were cases, and 85% of these had advanced AMD. Our studies extend the catalog of AMD associated loci, help identify individuals at high risk of disease, and provide clues about underlying cellular pathways that should eventually lead to new therapies.


Asunto(s)
Predisposición Genética a la Enfermedad , Lipoproteínas HDL/metabolismo , Degeneración Macular/genética , Inhibidor Tisular de Metaloproteinasa-3/genética , Alelos , Estudios de Casos y Controles , Mapeo Cromosómico , Factor I de Complemento/genética , Variación Genética , Estudio de Asociación del Genoma Completo , Genotipo , Humanos , Polimorfismo de Nucleótido Simple , Análisis de Regresión , Riesgo , Inhibidor Tisular de Metaloproteinasa-3/fisiología
12.
PLoS Genet ; 5(9): e1000660, 2009 Sep.
Artículo en Inglés | MEDLINE | ID: mdl-19779542

RESUMEN

Myopia is one of the most common ocular disorders worldwide. Pathological myopia, also called high myopia, comprises 1% to 5% of the general population and is one of the leading causes of legal blindness in developed countries. To identify genetic determinants associated with pathological myopia in Japanese, we conducted a genome-wide association study, analyzing 411,777 SNPs with 830 cases and 1,911 general population controls in a two-stage design (297 cases and 934 controls in the first stage and 533 cases and 977 controls in the second stage). We selected 22 SNPs that showed P-values smaller than 10(-4) in the first stage and tested them for association in the second stage. The meta-analysis combining the first and second stages identified an SNP, rs577948, at chromosome 11q24.1, which was associated with the disease (P = 2.22x10(-7) and OR of 1.37 with 95% confidence interval: 1.21-1.54). Two genes, BLID and LOC399959, were identified within a 200-kb DNA encompassing rs577948. RT-PCR analysis demonstrated that both genes were expressed in human retinal tissue. Our results strongly suggest that the region at 11q24.1 is a novel susceptibility locus for pathological myopia in Japanese.


Asunto(s)
Cromosomas Humanos Par 11/genética , Predisposición Genética a la Enfermedad , Estudio de Asociación del Genoma Completo , Miopía Degenerativa/genética , Pueblo Asiatico/genética , Femenino , Regulación de la Expresión Génica , Marcadores Genéticos , Genoma Humano/genética , Células HeLa , Humanos , Japón , Desequilibrio de Ligamiento/genética , Masculino , Persona de Mediana Edad , Polimorfismo de Nucleótido Simple/genética , Retina/metabolismo , Retina/patología
13.
Ophthalmology ; 118(7): 1408-15, 2011 Jul.
Artículo en Inglés | MEDLINE | ID: mdl-21439646

RESUMEN

PURPOSE: To investigate whether photodynamic therapy (PDT) outcomes of polypoidal choroidal vasculopathy (PCV) are related to baseline clinical characteristics, smoking history, or genetic factors by analyzing the retreatment-free period after the first PDT. DESIGN: Retrospective cohort study. PARTICIPANTS: The study consisted of 167 patients with PCV who underwent PDT as their first treatment. METHODS: We targeted 638 single nucleotide polymorphisms (SNPs) in 42 possible susceptible genes for age-related macular degeneration to evaluate their relation to the effectiveness of PDT for PCV. For this evaluation, we used 2 methods: (1) survival analysis, with the retreatment-free period as the target; and (2) logistic regression test between the need for additional therapy within 3 months after the first PDT and the genotypes, with age, gender, smoking status, and greatest linear dimension (GLD) at baseline as covariates. The contributions of smoking status and GLD at baseline for the retreatment-free period also were evaluated. Contributions of these factors to visual prognosis were evaluated for 1 year after PDT. MAIN OUTCOME MEASURES: Retreatment-free period after the first PDT for PCV. Secondary outcome measures included correlation of the susceptible factor to the retreatment requirement within the 3-month follow-up and the mean visual acuity change. RESULTS: In survival analyses, SERPINF1 rs12603825 showed a significant association with the retreatment-free period after the first PDT; those patients homozygous for the minor allele A of rs12603825 received additional treatment after PDT within significantly shorter times than those with other genotypes (P = 0.0038). There was no significant difference in the retreatment-free period between baseline GLD and smoking status. Retreatment within 3 months was required significantly more in patients with the AA genotype, even after taking into consideration the effect of clinical characteristics (age, gender), baseline PCV lesion size, and smoking status (P = 0.0027). Furthermore, patients with the AA genotype showed significantly worse visual prognosis after PDT (P = 0.013). CONCLUSIONS: Pigment epithelium-derived factor (SERPINF1 or PEDF) polymorphisms may influence the initial response to and visual prognosis after PDT for PCV. Our findings may lead to understanding the pathogenesis of PCV and modification of the effects of PDT.


Asunto(s)
Enfermedades de la Coroides/genética , Proteínas del Ojo/genética , Variación Genética , Factores de Crecimiento Nervioso/genética , Fotoquimioterapia , Pólipos/genética , Serpinas/genética , Enfermedades Vasculares/genética , Anciano , Coroides/irrigación sanguínea , Enfermedades de la Coroides/tratamiento farmacológico , Estudios de Cohortes , Femenino , Estudios de Seguimiento , Humanos , Masculino , Polimorfismo de Nucleótido Simple , Pólipos/tratamiento farmacológico , Pronóstico , Retratamiento , Estudios Retrospectivos , Fumar , Tasa de Supervivencia , Factores de Tiempo , Resultado del Tratamiento , Enfermedades Vasculares/tratamiento farmacológico
14.
Sci Rep ; 11(1): 4681, 2021 02 25.
Artículo en Inglés | MEDLINE | ID: mdl-33633220

RESUMEN

Retinitis pigmentosa (RP) is a heterogenous hereditary disorder leading to blindness. Despite using next-generation sequencing technologies, causal variants in about 60% of RP cases remain unknown. The heterogeneous genetic inheritance pattern makes it difficult to pinpoint causal variants. Besides, rare penetrating variants are hardly observed in general case-control studies. Thus, a family-based analysis, specifically in a consanguineous family, is a clinically and genetically valuable approach for RP. We analyzed a Japanese consanguineous family with a member suffering from RP with a typical autosomal recessive pattern. We sequenced five direct descendants and spouse using Whole-exome sequencing (WES) and Whole-genome sequencing (WGS). We identified a homozygous pathogenic missense variant in CNGA1 (NM_000087.3, c.839G > A, p.Arg280His) in the proband, while we found no homozygous genotypes in the other family members. CNGA1 was previously reported to be associated with RP. We confirmed the genotypes by the Sanger sequencing. Additionally, we assessed the homozygous genotype in the proband for the possibility of a founder mutation using homozygosity analysis. Our results suggested the two copies of the variant derived from a founder mutation. In conclusion, we found the homozygotes for c.839G > A in CNGA1 as causal for RP.


Asunto(s)
Canales Catiónicos Regulados por Nucleótidos Cíclicos/genética , Homocigoto , Retinitis Pigmentosa/genética , Consanguinidad , Femenino , Humanos , Japón , Masculino , Mutación Missense , Linaje , Secuenciación Completa del Genoma
15.
Invest Ophthalmol Vis Sci ; 61(12): 25, 2020 10 01.
Artículo en Inglés | MEDLINE | ID: mdl-33107904

RESUMEN

Purpose: Disruption of proteostasis is a key event in many neurodegenerative diseases. Heat shock proteins (HSPs) participate in multiple functions associated with intracellular transport and proteostasis. We evaluated the effect of augmented HSP70 expression in mutant photoreceptors of mouse retinal degeneration models to test the hypothesis that failure to sustain HSP70 expression contributes to photoreceptor cell death. Methods: We examined HSP70 expression in retinas of wild-type and mutant mice by RNA and protein analysis. A transgenic mouse line, TgCrx-Hspa1a-Flag, was generated to express FLAG-tagged full-length HSP70 protein under control of a 2.3 kb mouse Crx promoter. This line was crossed to three distinct retinal degeneration mouse models. Retinal structure and function were evaluated by histology, immunohistochemistry, and electroretinography. Results: In seven different mouse models of retinal degeneration, we detected transient elevation of endogenous HSP70 expression at early stages, followed by a dramatic reduction as cell death ensues, suggesting an initial adaptive response to cellular stress. Augmented expression of HSP70 in RHOT17M mice, in which mutant rhodopsin is misfolded, marginally improved photoreceptor survival, whereas elevated HSP70 led to more severe retinal degeneration in rd10 mutants that produce a partially functional PDE6B. In Rpgrip1-/- mice that display a ciliary defect, higher HSP70 had no impact on photoreceptor survival or function. Conclusions: HSP70 overexpression has divergent effects in photoreceptors determined, at least in part, by the nature of the mutant protein each model carries. Additional investigations on HSP pathways and associated chaperone networks in photoreceptors are needed before designing therapeutic strategies targeting proteostasis.


Asunto(s)
Regulación de la Expresión Génica/fisiología , Proteínas HSP70 de Choque Térmico/genética , Degeneración Retiniana/metabolismo , Células Fotorreceptoras Retinianas Bastones/metabolismo , Animales , Supervivencia Celular , Modelos Animales de Enfermedad , Electrorretinografía , Immunoblotting , Ratones , Ratones Endogámicos C57BL , Ratones Transgénicos , Microscopía Fluorescente , Retina/fisiopatología , Degeneración Retiniana/fisiopatología , Células Fotorreceptoras Retinianas Bastones/fisiología
16.
Sci Rep ; 10(1): 4251, 2020 03 06.
Artículo en Inglés | MEDLINE | ID: mdl-32144342

RESUMEN

In glaucoma, retinal ganglion cells are damaged, leading to the progressive constriction of the visual field. We have previously shown that the valosin-containing protein (VCP) modulators, Kyoto University Substance (KUS)121 and KUS187, prevent the death of retinal ganglion cells in animal models of glaucoma, including the one generated by N-methyl-D-aspartate (NMDA)-induced neurotoxicity. KUSs appeared to avert endoplasmic reticulum (ER) stress by maintaining ATP levels, resulting in the protection of ganglion cells from cell death. To further elucidate the protective mechanisms of KUSs, we examined gene expression profiles in affected ganglion cells. We first injected KUS-treated mice with NMDA and then isolated the affected retinal ganglion cells using fluorescence-activated cell sorting. Gene expression in the cells was quantified using a next-generation sequencer. Resultantly, we found that KUS121 upregulated several genes involved in energy metabolism. In addition, we observed the upregulation of Zfp667, which has been reported to suppress apoptosis-related genes and prevent cell death. These results further support the suitability of KUS121 as a therapeutic drug in protecting retinal ganglion cells in ophthalmic disorders, such as glaucoma.


Asunto(s)
Susceptibilidad a Enfermedades , Enfermedades de la Retina/etiología , Enfermedades de la Retina/metabolismo , Células Ganglionares de la Retina/metabolismo , Transcriptoma , Proteína que Contiene Valosina/genética , Proteína que Contiene Valosina/metabolismo , Enfermedad Aguda , Animales , Apoptosis , Biomarcadores , Biología Computacional/métodos , Modelos Animales de Enfermedad , Metabolismo Energético , Perfilación de la Expresión Génica , Ontología de Genes , Inmunofenotipificación , Redes y Vías Metabólicas , Ratones , N-Metilaspartato/efectos adversos , Enfermedades de la Retina/patología
17.
Am J Ophthalmol ; 145(3): 582-585, 2008 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-18201684

RESUMEN

PURPOSE: To investigate the contribution of two single-nucleotide polymorphisms (SNPs) of the lysyl oxidase-like 1 (LOXL1) gene, recently shown to be associated with exfoliation syndrome (XFS) and exfoliation glaucoma (XFG) in the Nordic population, to the occurrence of XFS and XFG in the Japanese population. DESIGN: Case-control association study. METHODS: A total of 59 unrelated Japanese individuals with XFS, 27 XFG patients, and 190 population-based controls were recruited. The SNPs rs1048661 (R141L) and rs3825942 (G153D) in the LOXL1 gene were genotyped directly. Association tests were performed for the two SNPs and inferred haplotypes. RESULTS: The frequency of the G allele in rs1048661, reportedly a functional risk allele in White persons, existed in only 0.8% of Japanese XFS cases, but occurred with much higher frequency in controls (46.0%) and yielded a P value of 3.0x10(-19), and the odds ratio for the T allele in rs1048661 was 99.8 (95% confidence interval, 13.8 to 722). For rs3825942, the frequency of the G allele, which is another possible risk allele in White persons with XFS, was 1.000 vs 0.857 in the controls (P=1.4x10(-5)). The most frequent haplotype in Japanese XFS patients was haplotype (T,G) (99.2%). The (G,G) haplotype, which generates the highest risk in White persons, was present in only a small percentage of Japanese XFS cases (0.8%). CONCLUSIONS: The SNPs rs1048661 and rs3825942 of the LOXL1 gene seem to be highly associated with XFS in the Japanese population, but a different polymorphism of LOXL1 may cause the development of XFS in the Japanese population.


Asunto(s)
Aminoácido Oxidorreductasas/genética , Síndrome de Exfoliación/genética , Glaucoma de Ángulo Abierto/genética , Polimorfismo de Nucleótido Simple/genética , Adulto , Anciano , Alelos , Pueblo Asiatico/etnología , Estudios de Casos y Controles , Síndrome de Exfoliación/etnología , Femenino , Glaucoma de Ángulo Abierto/etnología , Haplotipos , Humanos , Japón/epidemiología , Masculino , Reacción en Cadena de la Polimerasa
18.
Retina ; 28(10): 1493-501, 2008.
Artículo en Inglés | MEDLINE | ID: mdl-18667957

RESUMEN

PURPOSE: To describe the clinical characteristics of macular complications on the border of an inferior staphyloma associated with tilted disk syndrome. METHODS: We reviewed retrospectively the medical records of 32 consecutive eyes of 20 patients with tilted disk syndrome and an inferior staphyloma lying across the macula. RESULTS: In 21 (66%) eyes, fluorescein angiography showed window defects on the border of the staphyloma, where the early phase of indocyanine green angiography showed hypofluorescence due to atrophy of the choriocapillaris. On the late phase of indocyanine green angiography, 19 eyes (59%) showed hyperfluorescence along the border of the staphyloma, which often extended beyond the area of the window defect. Of the 32 eyes, 25 (78%) had macular complications: polypoidal choroidal vasculopathy in 7 (22%), classic choroidal neovascularization in 1 (3%), focal serous retinal detachment without polypoidal choroidal vasculopathy or choroidal neovascularization in 13 (41%), and atrophy of the retinal pigment epithelium alone in 4 (13%). Visual acuity in eyes with polypoidal choroidal vasculopathy or choroidal neovascularization was significantly worse than that in eyes with other complications (P < 0.001). CONCLUSIONS: Eyes with tilted disk syndrome often have macular complications on the border of the inferior staphyloma, which can cause severe visual loss.


Asunto(s)
Neovascularización Coroidal/etiología , Anomalías del Ojo/complicaciones , Mácula Lútea/patología , Disco Óptico/anomalías , Enfermedades de la Retina/etiología , Adulto , Anciano , Anciano de 80 o más Años , Coroides/irrigación sanguínea , Neovascularización Coroidal/diagnóstico , Colorantes , Dilatación Patológica , Anomalías del Ojo/diagnóstico , Femenino , Angiografía con Fluoresceína , Humanos , Verde de Indocianina , Masculino , Persona de Mediana Edad , Disco Óptico/patología , Enfermedades Vasculares Periféricas/diagnóstico , Enfermedades Vasculares Periféricas/etiología , Enfermedades de la Retina/diagnóstico , Estudios Retrospectivos , Síndrome , Tomografía de Coherencia Óptica , Agudeza Visual/fisiología
19.
Clin Exp Ophthalmol ; 36(5): 437-42, 2008 Jul.
Artículo en Inglés | MEDLINE | ID: mdl-18939352

RESUMEN

BACKGROUND: Typical exudative age-related macular degeneration (AMD) and polypoidal choroidal vasculopathy (PCV) are two of the major macular diseases found in Asians. Although genomic studies have shown a contribution by CFH and LOC387715/HTRA1 polymorphisms to the development of these two diseases, the correlation of the clinical phenotypes to these genotypes has not been determined in Asian patients. METHODS: The prevalence of the CFH Y402H and HTRA1 rs11200638 genotypes was determined in 116 patients with typical exudative AMD and in 204 patients with PCV. Potential correlations of these polymorphisms were tested retrospectively and cross-sectionally for bilaterality of the disease, final visual acuity and the greatest linear dimension of the choroidal neovascular (CNV) lesion. RESULTS: There was no significant difference in the incidence of CFH Y402H (P = 0.598) and HTRA1 rs11200638 (P = 0.290) between eyes with typical exudative AMD and with PCV. There was a significant association between the lesion size and HTRA1 rs11200638. For eyes with typical AMD, the size of the lesion (6363 +/- 2837 microm) was significantly larger in the high-risk homozygous group (AA), than in the low-risk homozygous group (GG) (3866 +/- 1947 microm; P = 0.0003). The same tendency was observed for the size of the lesion in PCV cases (homozygous group: 6347 +/- 2673 microm, non-risk homozygous group: 4405 +/- 2066 microm, P = 1.3 x 10(-5). CONCLUSIONS: A common genetic background may exist between typical exudative AMD and PCV patients. Among the patients with these two clinical entities, those with a homozygous HTRA1 rs11200638 risk allele had larger CNV lesions.


Asunto(s)
Pueblo Asiatico/genética , Neovascularización Coroidal/genética , Exudados y Transudados/metabolismo , Degeneración Macular/genética , Polimorfismo Genético , Serina Endopeptidasas/genética , Anciano , Anciano de 80 o más Años , Alelos , Neovascularización Coroidal/patología , Neovascularización Coroidal/fisiopatología , Factor H de Complemento/genética , Estudios Transversales , Femenino , Predisposición Genética a la Enfermedad , Genotipo , Serina Peptidasa A1 que Requiere Temperaturas Altas , Homocigoto , Humanos , Degeneración Macular/metabolismo , Masculino , Fenotipo , Estudios Retrospectivos , Visión Binocular , Agudeza Visual
20.
Ophthalmology ; 114(12): 2197-207, 2007 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-17507096

RESUMEN

OBJECTIVE: To describe the 3-dimensional (3-D) imaging of the pathologic changes in the foveal photoreceptor layer in eyes with central serous chorioretinopathy (CSC) using high-speed optical coherence tomography (OCT). DESIGN: Prospective observational case series. PARTICIPANTS: Twenty-seven eyes of 27 consecutive patients with various stages of CSC. METHODS: A prototype high-speed OCT system was fabricated for patient examinations based on Fourier domain OCT. The system had a sensitivity of 98 dB, a tissue axial resolution of 4.3 mum, and an acquisition rate of approximately 18700 axial scans per second. Three-dimensional imaging was performed based on a raster-scan protocol. MAIN OUTCOME MEASURES: Anatomic features of CSC distinguished by 3-D OCT. RESULTS: A line corresponding to backreflection from the external limiting membrane (ELM) was visible in images from eyes with all stages of CSC, including 6 in the acute, 5 in the chronic, and 9 in the recurrent phase of retinal detachment and 11 examined in the quiescent phase (including 4 reexamined after reattachment). Backreflection from the photoreceptor inner and outer segment junction (IS/OS) was missing before but present after reattachment. The ELM line bordered the photoreceptor nuclear and inner segment layers, clearly showing that CSC primarily alters the outer segment (OS) layer. Punctate areas of intense reflectivity were observed more frequently in the OS layer of detached retinas in cases of chronic or recurrent versus acute CSC (P<0.05, chi-square test). Of 11 eyes with reattached macula, 3 eyes with large defects in the subfoveal IS/OS had poor visual acuity (VA), and 8 eyes with small or no defects had good VA (P<0.05 and P<0.001, chi-square test). Decreases in foveal full and outer thickness were associated with VA loss (P<0.05 and P<0.001, Spearman rank correlation test). CONCLUSIONS: Three-dimensional OCT imaging delineates the microstructural changes that occur within the photoreceptor layers and demonstrates the spatial relationship between the laterally spreading or scattering microstructures and the fovea in eyes with CSC. Visualization of the 3-D relationship between the ELM and each photoreceptor layer before and after macular reattachment facilitates understanding of anatomic and vision changes that result from CSC.


Asunto(s)
Enfermedades de la Coroides/diagnóstico , Fóvea Central/patología , Imagenología Tridimensional/métodos , Células Fotorreceptoras de Vertebrados/patología , Enfermedades de la Retina/diagnóstico , Tomografía de Coherencia Óptica/métodos , Enfermedad Aguda , Adulto , Anciano , Enfermedad Crónica , Femenino , Análisis de Fourier , Humanos , Masculino , Persona de Mediana Edad , Estudios Prospectivos , Recurrencia , Sensibilidad y Especificidad , Agudeza Visual
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