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1.
MMWR Morb Mortal Wkly Rep ; 69(27): 887-892, 2020 Jul 10.
Artículo en Inglés | MEDLINE | ID: mdl-32644986

RESUMEN

Meat and poultry processing facilities face distinctive challenges in the control of infectious diseases, including coronavirus disease 2019 (COVID-19) (1). COVID-19 outbreaks among meat and poultry processing facility workers can rapidly affect large numbers of persons. Assessment of COVID-19 cases among workers in 115 meat and poultry processing facilities through April 27, 2020, documented 4,913 cases and 20 deaths reported by 19 states (1). This report provides updated aggregate data from states regarding the number of meat and poultry processing facilities affected by COVID-19, the number and demographic characteristics of affected workers, and the number of COVID-19-associated deaths among workers, as well as descriptions of interventions and prevention efforts at these facilities. Aggregate data on confirmed COVID-19 cases and deaths among workers identified and reported through May 31, 2020, were obtained from 239 affected facilities (those with a laboratory-confirmed COVID-19 case in one or more workers) in 23 states.* COVID-19 was confirmed in 16,233 workers, including 86 COVID-19-related deaths. Among 14 states reporting the total number of workers in affected meat and poultry processing facilities (112,616), COVID-19 was diagnosed in 9.1% of workers. Among 9,919 (61%) cases in 21 states with reported race/ethnicity, 87% occurred among racial and ethnic minority workers. Commonly reported interventions and prevention efforts at facilities included implementing worker temperature or symptom screening and COVID-19 education, mandating face coverings, adding hand hygiene stations, and adding physical barriers between workers. Targeted workplace interventions and prevention efforts that are appropriately tailored to the groups most affected by COVID-19 are critical to reducing both COVID-19-associated occupational risk and health disparities among vulnerable populations. Implementation of these interventions and prevention efforts† across meat and poultry processing facilities nationally could help protect workers in this critical infrastructure industry.


Asunto(s)
Infecciones por Coronavirus/epidemiología , Brotes de Enfermedades , Industria de Procesamiento de Alimentos , Enfermedades Profesionales/epidemiología , Neumonía Viral/epidemiología , Adulto , Animales , COVID-19 , Femenino , Humanos , Masculino , Carne , Persona de Mediana Edad , Pandemias , Aves de Corral , Estados Unidos/epidemiología
2.
Emerg Infect Dis ; 20(10): 1697-704, 2014 Oct.
Artículo en Inglés | MEDLINE | ID: mdl-25396258

RESUMEN

Venezuela had the highest number of human malaria cases in Latin American before 1936. During 1891­1920,malaria was endemic to >600,000 km2 of this country; malaria death rates led to major population decreases during 1891­1920. No pathogen, including the influenza virus that caused the 1918 pandemic, caused more deaths than malaria during 1905­1945. Early reports of malaria eradication in Venezuela helped spark the world's interest in global eradication. We describe early approaches to malaria epidemiology in Venezuela and how this country developed an efficient control program and an approach to eradication.Arnoldo Gabaldón was a key policy maker during this development process. He directed malaria control in Venezuela from the late 1930s to the end of the 1970s and contributed to malaria program planning of the World Health Organization.We discuss how his efforts helped reduce the incidence of malaria in Venezuela and how his approach diverged from World Health Organization guidelines.


Asunto(s)
Malaria/historia , Malaria/prevención & control , Política de Salud/historia , Historia del Siglo XIX , Historia del Siglo XX , Humanos , Insecticidas , Control de Mosquitos/métodos , Venezuela/epidemiología
3.
Malar J ; 12: 303, 2013 Aug 30.
Artículo en Inglés | MEDLINE | ID: mdl-24001096

RESUMEN

Malaria has been part of Peruvian life since at least the 1500s. While Peru gave the world quinine, one of the first treatments for malaria, its history is pockmarked with endemic malaria and occasional epidemics. In this review, major increases in Peruvian malaria incidence over the past hundred years are described, as well as the human factors that have facilitated these events, and concerted private and governmental efforts to control malaria. Political support for malaria control has varied and unexpected events like vector and parasite resistance have adversely impacted morbidity and mortality. Though the ready availability of novel insecticides like DDT and efficacious medications reduced malaria to very low levels for a decade after the post eradication era, malaria reemerged as an important modern day challenge to Peruvian public health. Its reemergence sparked collaboration between domestic and international partners towards the elimination of malaria in Peru.


Asunto(s)
Antimaláricos/uso terapéutico , Control de Enfermedades Transmisibles/historia , Control de Enfermedades Transmisibles/métodos , Malaria/epidemiología , Malaria/historia , Antimaláricos/historia , Quimioterapia/historia , Política de Salud , Historia del Siglo XX , Humanos , Malaria/tratamiento farmacológico , Malaria/prevención & control , Perú/epidemiología , Quinina
4.
Malar J ; 11: 77, 2012 Mar 22.
Artículo en Inglés | MEDLINE | ID: mdl-22439637

RESUMEN

BACKGROUND: Understanding the origin and spread of mutations associated with drug resistance, especially in the context of combination therapy, will help guide strategies to halt and prevent the emergence of resistance. Unfortunately, studies have assessed these complex processes when resistance is already highly prevalent. Even further, information on the evolutionary dynamics leading to multidrug-resistant parasites is scattered and limited to areas with low or seasonal malaria transmission. This study describes the dynamics of strong selection for mutations conferring resistance against sulphadoxine-pyrimethamine (SP), a combination therapy, in western Kenya between 1992 and 1999, just before SP became first-line therapy (1999). Importantly, the study is based on longitudinal data, which allows for a comprehensive analysis that contrasts with previous cross-sectional studies carried out in other endemic regions. METHODS: This study used 236 blood samples collected between 1992 and 1999 in the Asembo Bay area of Kenya. Pyrosequencing was used to determine the alleles of dihydrofolate reductase (dhfr) and dihydropterote synthase (dhps) genes. Microsatellite alleles spanning 138 kb around dhfr and dhps, as well as, neutral markers spanning approximately 100 kb on chromosomes 2 and 3 were characterized. RESULTS: By 1992, the South-Asian dhfr triple mutant was already spreading, albeit in low frequency, in this holoendemic Kenyan population, prior to the use of SP as a first-line therapy. Additionally, dhfr triple mutant alleles that originated independently from the predominant Southeast Asian lineage were present in the sample set. Likewise, dhps double mutants were already present as early as 1992. There is evidence for soft selective sweeps of two dhfr mutant alleles and the possible emergence of a selective sweep of double mutant dhps alleles between 1992 and 1997. The longitudinal structure of the dataset allowed estimation of selection pressures on various dhfr and dhps mutants relative to each other based on a theoretical model tailored to P. falciparum. The data indicate that drug selection acted differently on the resistant alleles of dhfr and dhps, as evidenced by fitness differences. Thus a combination drug therapy such as SP, by itself, does not appear to select for "multidrug"-resistant parasites in areas with high recombination rate. CONCLUSIONS: The complexity of these observations emphasizes the importance of population-based studies to evaluate the effects of strong drug selection on Plasmodium falciparum populations.


Asunto(s)
Antimaláricos/farmacología , Dihidropteroato Sintasa/genética , Malaria Falciparum/tratamiento farmacológico , Plasmodium falciparum/genética , Proteínas Protozoarias/genética , Pirimetamina/farmacología , Sulfadoxina/farmacología , Tetrahidrofolato Deshidrogenasa/genética , Alelos , Combinación de Medicamentos , Resistencia a Medicamentos , Quimioterapia Combinada , Humanos , Kenia , Estudios Longitudinales , Malaria Falciparum/parasitología , Repeticiones de Microsatélite , Mutación , Filogeografía , Plasmodium falciparum/efectos de los fármacos , Plasmodium falciparum/enzimología , Recombinación Genética , Selección Genética
5.
J Infect Dis ; 203(2): 220-7, 2011 Jan 15.
Artículo en Inglés | MEDLINE | ID: mdl-21288822

RESUMEN

BACKGROUND: In 2005, Ghana adopted artemisinin-based combination therapy (ACT) for primary treatment of falciparum malaria. A comprehensive study of the drug-resistance-associated mutations and their genetic lineages will lead to a better understanding of the evolution of antimalarial drug resistance in this region. METHODS: The pfcrt, pfmdr1, dhps, and dhfr mutations associated with chloroquine (CQ) and sulfadoxine-pyrimethamine (SP) resistance and the microsatellite loci flanking these genes were genotyped in Plasmodium falciparum isolates from Ghana. RESULTS: The prevalence of mutations associated with both CQ and SP resistance was high in Ghana. However, we observed a decrease in prevalence of the pfcrt K76T mutation in northern Ghana after the change in drug policy from CQ to ACT. Analysis of genetic diversity and differentiation at microsatellite loci flanking all 4 genes indicated that they have been under strong selection, because of CQ and SP use. The triple-mutant pfcrt and dhfr alleles in Ghana were derived from Southeast Asia, whereas the double-mutant dhfr, dhps, and pfmdr1 alleles were of African lineage. CONCLUSION: Because of the possible role of pfmdr1 in amodiaquine and mefloquine resistance, demonstrating selection on pfmdr1 and defining lineages of resistant alleles in an African population holds great importance.


Asunto(s)
Alelos , Antimaláricos/farmacología , Resistencia a Medicamentos , Malaria Falciparum/parasitología , Plasmodium falciparum/efectos de los fármacos , Plasmodium falciparum/genética , Sustitución de Aminoácidos , Evolución Biológica , Preescolar , Cloroquina/farmacología , ADN Protozoario/genética , Dihidropteroato Sintasa/genética , Combinación de Medicamentos , Evolución Molecular , Genotipo , Ghana , Humanos , Lactante , Recién Nacido , Proteínas de Transporte de Membrana/genética , Repeticiones de Microsatélite , Proteínas Asociadas a Resistencia a Múltiples Medicamentos/genética , Mutación Missense , Plasmodium falciparum/clasificación , Plasmodium falciparum/aislamiento & purificación , Proteínas Protozoarias/genética , Pirimetamina/farmacología , Sulfadoxina/farmacología , Tetrahidrofolato Deshidrogenasa/genética
6.
Antimicrob Agents Chemother ; 53(5): 2042-51, 2009 May.
Artículo en Inglés | MEDLINE | ID: mdl-19258269

RESUMEN

Monitoring changes in the frequencies of drug-resistant and -sensitive genotypes can facilitate in vivo clinical trials to assess the efficacy of drugs before complete failure occurs. Peru changed its national treatment policy for uncomplicated malaria to artesunate (ART)-plus-mefloquine (MQ) combination therapy in the Amazon basin in 2001. We genotyped isolates collected in 1999 and isolates collected in 2006 to 2007 for mutations in the Plasmodium falciparum dihydrofolate reductase (Pfdhfr) and dihydropteroate synthase (Pfdhps) genes, multidrug resistance gene 1 (Pfmdr-1), the chloroquine (CQ) resistance transporter gene (Pfcrt), and the Ca(2+) ATPase gene (PfATP6); these have been shown to be involved in resistance to sulfadoxine-pyrimethamine (SP), MQ, CQ, and possibly ART, respectively. Microsatellite haplotypes around the Pfdhfr, Pfdhps, Pfcrt, and Pfmdr-1 loci were also determined. There was a significant decline in the highly SP resistant Pfdhfr and Pfdhps genotypes from 1999 to 2006. In contrast, a CQ-resistant Pfcrt genotype increased in frequency during the same period. Among five different Pfmdr-1 allelic forms noted in 1999, two genotypes increased in frequency while one genotype decreased by 2006. We also noted previously undescribed polymorphisms in the PfATP6 gene as well as an increase in the frequency of a deletion mutant during this period. In addition, microsatellite analysis revealed that the resistant Pfdhfr, Pfdhps, and Pfcrt genotypes have each evolved from a single founder haplotype, while Pfmdr-1 genotypes have evolved from at least two independent haplotypes. Importantly, this study demonstrates that the Peruvian triple mutant Pfdhps genotypes are very similar to those found in other parts of South America.


Asunto(s)
Antimaláricos , Resistencia a Medicamentos/genética , Política de Salud , Malaria Falciparum , Plasmodium falciparum/efectos de los fármacos , Proteínas Protozoarias/genética , Animales , Antimaláricos/farmacología , Antimaláricos/uso terapéutico , Genotipo , Haplotipos , Humanos , Malaria Falciparum/tratamiento farmacológico , Malaria Falciparum/epidemiología , Malaria Falciparum/parasitología , Repeticiones de Microsatélite/genética , Mutación , Pruebas de Sensibilidad Parasitaria , Perú/epidemiología , Plasmodium falciparum/genética , Plasmodium falciparum/aislamiento & purificación
7.
Vector Borne Zoonotic Dis ; 7(3): 437-43, 2007.
Artículo en Inglés | MEDLINE | ID: mdl-17767414

RESUMEN

We measured mosquito landing rates on adult and nestling American robins at nests with infrared cameras in Washington, D.C., and Maryland, United States. Mosquitoes landed on nesting robins almost exclusively between dusk and dawn. The mean number of mosquito landings per night was higher for adults (123.3 +/- SE 32.8) than nestlings (37.26 +/- 14.8). The fraction of mosquitoes landing at a nest on nestlings increased with decreases in adult brooding. Oral swabs from nestlings at these and 13 other robin, Gray catbird, and house finch nests were negative for West Nile virus (WNV). These results show that landing rates were higher on adults and that parental brooding reduces the landing rates of mosquitoes on nestlings.


Asunto(s)
Culex/fisiología , Interacciones Huésped-Parásitos/fisiología , Insectos Vectores/fisiología , Pájaros Cantores/parasitología , Animales , District of Columbia , Conducta Alimentaria/fisiología , Femenino , Mordeduras y Picaduras de Insectos/veterinaria , Maryland , Comportamiento de Nidificación/fisiología , Fotograbar , Pájaros Cantores/virología , Factores de Tiempo , Fiebre del Nilo Occidental/transmisión , Virus del Nilo Occidental/aislamiento & purificación
8.
PLoS One ; 10(7): e0131967, 2015.
Artículo en Inglés | MEDLINE | ID: mdl-26132731

RESUMEN

The objective of this study was to develop a canonical, parsimoniously-informative SNP panel for subtyping Shiga-toxin producing Escherichia coli (STEC) O157:H7 that would be consistent with epidemiological, PFGE, and MLVA clustering of human specimens. Our group had previously identified 906 putative discriminatory SNPs, which were pared down to 391 SNPs based on their prevalence in a test set. The 391 SNPs were screened using a high-throughput form of TaqMan PCR against a set of clinical isolates that represent the most diverse collection of O157:H7 isolates from outbreaks and sporadic cases examined to date. Another 30 SNPs identified by others were also screened using the same method. Two additional targets were tested using standard TaqMan PCR endpoint analysis. These 423 SNPs were reduced to a 32 SNP panel with the almost the same discriminatory value. While the panel partitioned our diverse set of isolates in a manner that was consistent with epidemiological data and PFGE and MLVA phylogenies, it resulted in fewer subtypes than either existing method and insufficient epidemiological resolution in 10 of 47 clusters. Therefore, another round of SNP discovery was undertaken using comparative genomic resequencing of pooled DNA from the 10 clusters with insufficient resolution. This process identified 4,040 potential SNPs and suggested one of the ten clusters was incorrectly grouped. After its removal, there were 2,878 SNPs, of which only 63 were previously identified and 438 occurred across multiple clusters. Among highly clonal bacteria like STEC O157:H7, linkage disequilibrium greatly limits the number of parsimoniously informative SNPs. Therefore, it is perhaps unsurprising that our panel accounted for the potential discriminatory value of numerous other SNPs reported in the literature. We concluded published O157:H7 SNPs are insufficient for effective epidemiological subtyping. However, the 438 multi-cluster SNPs we identified may provide the additional information required.


Asunto(s)
Escherichia coli O157/genética , Polimorfismo de Nucleótido Simple , Escherichia coli Shiga-Toxigénica/genética , Infecciones por Escherichia coli/microbiología , Humanos
9.
PLoS One ; 8(3): e58984, 2013.
Artículo en Inglés | MEDLINE | ID: mdl-23554964

RESUMEN

Previous work suggests that Brazilian Plasmodium falciparum has limited genetic diversity and a history of bottlenecks, multiple reintroductions due to human migration, and clonal expansions. We hypothesized that Brazilian P. falciparum would exhibit clonal structure. We examined isolates collected across two decades from Amapá, Rondônia, and Pará state (n = 190). By examining more microsatellites markers on more chromosomes than previous studies, we hoped to define the extent of low diversity, linkage disequilibrium, bottlenecks, population structure, and parasite migration within Brazil. We used retrospective genotyping of samples from the 1980s and 1990s to explore the population genetics of SP resistant dhfr and dhps alleles. We tested an existing hypothesis that the triple mutant dhfr mutations 50R/51I/108N and 51I/108N/164L developed in southern Amazon from a single origin of common or similar parasites. We found that Brazilian P. falciparum had limited genetic diversity and isolation by distance was rejected, which suggests it underwent bottlenecks followed by migration between sites. Unlike Peru, there appeared to be gene flow across the Brazilian Amazon basin. We were unable to divide parasite populations by clonal lineages and pairwise FST were common. Most parasite diversity was found within sites in the Brazilian Amazon, according to AMOVA. Our results challenge the hypothesis that triple mutant alleles arose from a single lineage in the Southern Amazon. SP resistance, at both the double and triple mutant stages, developed twice and potentially in different regions of the Brazilian Amazon. We would have required samples from before the 1980s to describe how SP resistance spread across the basin or describe the complex internal migration of Brazilian parasites after the colonization efforts of past decades. The Brazilian Amazon basin may have sufficient internal migration for drug resistance reported in any particular region to rapidly spread to other parts of basin under similar drug pressure.


Asunto(s)
Alelos , Resistencia a Medicamentos/genética , Plasmodium falciparum/efectos de los fármacos , Plasmodium falciparum/genética , Proteínas Protozoarias/genética , Evolución Biológica , Brasil , Variación Genética , Genotipo , Geografía , Humanos , Repeticiones de Microsatélite , Mutación , Tetrahidrofolato Deshidrogenasa/genética
10.
Sci Rep ; 3: 2797, 2013 Sep 30.
Artículo en Inglés | MEDLINE | ID: mdl-24077522

RESUMEN

The majority of malaria rapid diagnostic tests (RDTs) detect Plasmodium falciparum histidine-rich protein 2 (PfHRP2), encoded by the pfhrp2 gene. Recently, P. falciparum isolates from Peru were found to lack pfhrp2 leading to false-negative RDT results. We hypothesized that pfhrp2-deleted parasites in Peru derived from a single genetic event. We evaluated the parasite population structure and pfhrp2 haplotype of samples collected between 1998 and 2005 using seven neutral and seven chromosome 8 microsatellite markers, respectively. Five distinct pfhrp2 haplotypes, corresponding to five neutral microsatellite-based clonal lineages, were detected in 1998-2001; pfhrp2 deletions occurred within four haplotypes. In 2003-2005, outcrossing among the parasite lineages resulted in eight population clusters that inherited the five pfhrp2 haplotypes seen previously and a new haplotype; pfhrp2 deletions occurred within four of these haplotypes. These findings indicate that the genetic origin of pfhrp2 deletion in Peru was not a single event, but likely occurred multiple times.


Asunto(s)
Antígenos de Protozoos/genética , Eliminación de Gen , Parásitos/genética , Plasmodium falciparum/genética , Proteínas Protozoarias/genética , Animales , Teorema de Bayes , Análisis por Conglomerados , Haplotipos/genética , Humanos , Repeticiones de Microsatélite/genética , Perú , Fenotipo , Prevalencia
11.
PLoS One ; 6(9): e23486, 2011.
Artículo en Inglés | MEDLINE | ID: mdl-21949680

RESUMEN

Malaria has reemerged in many regions where once it was nearly eliminated. Yet the source of these parasites, the process of repopulation, their population structure, and dynamics are ill defined. Peru was one of malaria eradication's successes, where Plasmodium falciparum was nearly eliminated for two decades. It reemerged in the 1990s. In the new era of malaria elimination, Peruvian P. falciparum is a model of malaria reinvasion. We investigated its population structure and drug resistance profiles. We hypothesized that only populations adapted to local ecological niches could expand and repopulate and originated as vestigial populations or recent introductions. We investigated the genetic structure (using microsatellites) and drug resistant genotypes of 220 parasites collected from patients immediately after peak epidemic expansion (1999-2000) from seven sites across the country. The majority of parasites could be grouped into five clonal lineages by networks and AMOVA. The distribution of clonal lineages and their drug sensitivity profiles suggested geographic structure. In 2001, artesunate combination therapy was introduced in Peru. We tested 62 parasites collected in 2006-2007 for changes in genetic structure. Clonal lineages had recombined under selection for the fittest parasites. Our findings illustrate that local adaptations in the post-eradication era have contributed to clonal lineage expansion. Within the shifting confluence of drug policy and malaria incidence, populations continue to evolve through genetic outcrossing influenced by antimalarial selection pressure. Understanding the population substructure of P. falciparum has implications for vaccine, drug, and epidemiologic studies, including monitoring malaria during and after the elimination phase.


Asunto(s)
Resistencia a Medicamentos/genética , Malaria Falciparum/parasitología , Plasmodium falciparum/clasificación , Plasmodium falciparum/genética , Antimaláricos/farmacología , Antimaláricos/uso terapéutico , Dihidropteroato Sintasa/genética , Epidemias/prevención & control , Frecuencia de los Genes , Genotipo , Geografía , Haplotipos , Humanos , Desequilibrio de Ligamiento , Malaria Falciparum/epidemiología , Malaria Falciparum/prevención & control , Proteínas de Transporte de Membrana/genética , Repeticiones de Microsatélite/genética , Proteínas Asociadas a Resistencia a Múltiples Medicamentos/genética , Perú/epidemiología , Filogenia , Plasmodium falciparum/efectos de los fármacos , Crecimiento Demográfico , Proteínas Protozoarias/genética , Tetrahidrofolato Deshidrogenasa/genética
12.
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