Nanosilver gel as an endodontic alternative against Enterococcus faecalis in an in vitro root canal system in Mexican dental specimens.

Nanotechnology has become a research area with promising results for technological innovation. Endodontics can benefit from this field of research by increasing the success rate of the treatment, which currently ranges between 86% and 98% and has varied very little over the years. One of the causes of endodontic treatment failure is based on the presence of Enterococcus faecalis. The objective of this investigation is to evaluate the antibacterial effect of a gel preparation containing silver nanoparticles (Ag-NP) against E. faecalis present in the walls of the root canal. 60 extracted human uniradicular teeth that were instrumented with Wave One Gold (Denstplay/USA) and subsequently contaminated with Enterococcus faecalis. For antibacterial evaluation, intra-canal conducting was placed, and several groups were formed: a) Ag-NP 300 ug/MI gel; b) Ag-NP 500 ug/MI gel; c) Ca (OH) 2 (Ultracal from Ultradent/USA) and the control group. They were incubated at 37°C and a sample was taken every 24 h for 7 days. The Ag-NP gel showed antimicrobial activity against E. faecalis with a value of minimum inhibitory concentration and minimum bactericidal concentration of 300 g/ml and 900 g/ml, respectively. When the Ag-NP gel was used as an intra-canal conducting drug in an in-vitro model, its antimicrobial effect at 300 g/ml and 500 g/ml was equivalent to the action of Ca(OH)2.

The effect of Emdogain and 24% EDTA root conditioning on periodontal healing of replanted dog's teeth.

Controversies still exist as for the regenerative role of enamel matrix derivatives and the need for removal of the periodontal ligament in replanted teeth. The purpose of this study was to evaluate the effect of Emdogain and 24% ethylenediamine tetraacetic acid (EDTA) root conditioning on periodontal healing of replanted dog's teeth. Teeth were extracted, endodontically treated and preconditioned as follows: group 1, Emdogain; group 2, Emdogain + EDTA and group 3, EDTA. Teeth were replanted after 30 min extraoral time, splinted for 15 days and animals sacrificed after 8 weeks of observation. Histological evaluation was performed using hematoxylin/eosin and Masson trichrome and results scored based on previously reported criteria for histological evaluation. Replacement root resorption was histologically diagnosed in all groups except in the negative control. A parametric analysis showed no statistically significant differences between experimental groups. Root preconditioning with Emdogain alone or in combination with 24% EDTA showed no evidence of regeneration of collagen fibers and consequently did not prevent the development of replacement root resorption on replanted dog's teeth.