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BACKGROUND: Peripheral nerve defects (PND) often cause lifelong physical disability, and the available treatment options are often not satisfactory. PND are usually bridged with an autologous nerve transplant or a nerve guidance conduit (NGC), when coaptation as preferred technique is not possible. The aim of this experimental study was to determine the effectiveness of a novel NGC for regeneration in the treatment of PND. MATERIALS AND METHODS: A conduit made of gelatin with an innovative interior structure was tested for the repair of a 6-mm gap versus direct microsurgical suture repair without gap. RESULTS: We found that bridging the defect with this conduit was as effective as direct microsurgical coaptation without a defect. CONCLUSIONS: This nerve conduit, effective in bridging neural defects, appears as an alternative to autologous nerve grafts, avoiding the problems related to nerve graft harvesting, host-donor differences in diameter, mismatches in number and pattern of fascicles, cross-sectional shape and area, and morbidity of the donor area.
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Regeneración Nerviosa , Traumatismos de los Nervios Periféricos/cirugía , Prótesis e Implantes , Nervio Ciático/lesiones , Animales , Atrofia , Potenciales Evocados Motores , Análisis de la Marcha , Gelatina , Modelos Animales , Músculos/inervación , Músculos/patología , Músculos/fisiología , Regeneración Nerviosa/fisiología , Procedimientos Neuroquirúrgicos/métodos , Traumatismos de los Nervios Periféricos/fisiopatología , Diseño de Prótesis , Distribución Aleatoria , Nervio Ciático/fisiología , Porcinos , Porcinos Enanos , TitanioRESUMEN
Despite the popularity of a simultaneous application of dermal matrices and split-thickness skin grafts, scarce evidence exists about the process of revascularization involved. In this study, we aimed at analyzing the progression of revascularization by high-resolution episcopic microscopy (HREM) in a porcine excisional wound model. Following the surgical procedure creating 5 × 5 cm(2) full-thickness defects on the back, one area was covered with an autologous split-thickness skin graft alone (control group), the other with a collagen-elastin dermal matrix plus split-thickness skin graft (dermal matrix group). Two skin biopsies per each group and location were performed on day 5, 10, 15, and 28 postoperatively and separately processed for H&E as well as HREM. The dermal layer was thicker in the dermal matrix group vs. control on day 5 and 28. No differences were found for revascularization by conventional histology. In HREM, the dermal matrix did not appear to decelerate the revascularization process. The presence of the dermal matrix could be distinguished until day 15. By day 28, the structure of the dermal matrix could no longer be delineated and was replaced by autologous tissue. As assessed by conventional histology and confirmed by HREM, the revascularization process was comparable in both groups, notably with regard to the vertical ingrowth of sprouting vessels. The presented technique of HREM is a valuable addition for analyzing small vessel sprouting in dermal matrices in the future.
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Dermis/trasplante , Trasplante de Piel , Piel/patología , Cicatrización de Heridas , Animales , Modelos Animales de Enfermedad , Elastina , Femenino , Supervivencia de Injerto , Microscopía , Piel/irrigación sanguínea , Piel/lesiones , Porcinos , Trasplante AutólogoRESUMEN
INTRODUCTION: Even the most modern technology has failed to induce satisfactory functional regeneration of traumatically severed peripheral nerves. Delayed neural regeneration and in consequence, slower neural conduction seriously limit muscle function in the area supplied by the injured nerve. This study aimed to compare a new nerve coaptation system involving an innovative prosthesis with the classical clinical method of sutured nerve coaptation. Besides the time and degree of nerve regeneration, the influence of electrostimulation was also tested. METHODS: The sciatic nerve was severed in 14 female Göttingen minipigs with an average weight of 40.4 kg. The animals were randomized into 2 groups: One group received the new prosthesis and the other underwent microsurgical coaptation. In each group, according to the randomization a part of the animals received postoperative electrostimulation. Postoperative monitoring and the stimulation schedule covered a period of 9 months, during which axonal budding was evaluated monthly. RESULTS: The data from the pilot study indicate that results with the nerve prosthesis were comparable with those of conventional coaptation. CONCLUSION: The results indicate that implantation of the nerve prosthesis allows for good and effective neural regeneration. This new and simple treatment option for peripheral nerve injuries can be performed in any hospital with surgical facilities as it does not involve the demanding microsurgical suture technique that can only be performed in specialized centers.
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Regeneración Nerviosa/fisiología , Prótesis Neurales , Traumatismos de los Nervios Periféricos/cirugía , Nervios Periféricos/cirugía , Ingeniería de Tejidos/instrumentación , Potenciales de Acción/fisiología , Animales , Ingeniería Biomédica/instrumentación , Terapia por Estimulación Eléctrica , Femenino , Músculo Esquelético/fisiología , Proyectos Piloto , Porcinos , Porcinos EnanosRESUMEN
BACKGROUND: Surgical therapy for widespread first carpometacarpal (CMC1) arthritis permanently alters the physiologic anatomy of the hand. The injection of autologous substances into the thumb saddle joint could achieve temporary pain relief and delay surgical indications. This trial aimed to compare the pain-reducing effects of autologous fat and/or platelet-rich plasma (PRP) with saline 0.9% in the infiltration therapy of carpometacarpal arthritis of the thumb (CMC1 arthritis). METHODS: A blinded, randomized controlled trial was conducted. Ninety-five patients with CMC1 arthritis were included in the study. The mean follow-up period was 2 years. Participants were injected with 1.5 mL of autologous fat, PRP, autologous fat and PRP, or 0.9% saline solution into the CMC1 joint, depending on the group allocation. The primary outcome of this study was the evaluation of pain reduction in each treatment group. RESULTS: The combination of fat and PRP was the only treatment that resulted in a significantly greater reduction in pain compared with 0.9% saline ( P = 0.003). Similarly, fat and PRP in combination was the only therapy group to achieve clinically relevant Quick Disabilities of Arm, Shoulder, and Hand score reduction, and the only group that showed a significantly better Medical Outcomes Study 36-Item Short-Form Health Survey score than 0.9% saline ( P = 0.016). No major complications were noted. CONCLUSIONS: In addition to pain reduction, the combination of autologous fat and PRP yields a relevant improvement in hand function and a corresponding improvement in quality of life. This treatment seems to be a viable and safe alternative to short-acting glucocorticoids. CLINICAL QUESTION/LEVEL OF EVIDENCE: Therapeutic, II.
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Osteoartritis , Plasma Rico en Plaquetas , Humanos , Calidad de Vida , Solución Salina , Osteoartritis/cirugía , Glucocorticoides , Dolor , Resultado del TratamientoRESUMEN
BACKGROUND: Over 90% of patients with systemic sclerosis suffer from gastroesophageal reflux. Esophageal motility disturbances are associated with a reduced life quality and may force interstitial lung disease progression. We wanted to determine whether we can improve gastroesophageal reflux in these patients by esophageal stem-cell injection. METHODS: We performed a pilot study including eights patients with systemic sclerosis and symptomatic gastroesophageal reflux. Sampling of adipose tissue was performed by an experienced plastic surgeon under local anesthesia. The collected fat was injected into the submucosa of the distal esophagus, each time 1 ml in all four quadrants starting 2, 4 and 6 cm proximal to the Z line (ending up to a total volume of 12 ml). Before the intervention, 3, 6 and finally 12 months after the procedure, patients answered the Gastroesophageal Reflux Disease Health-Related Quality of Life Questionnaire (GERD HRQL) and a high-resolution manometry was performed to quantify changes in motility function. RESULTS: All patients showed an improvement in the GERD HRQL score after the stem-cell injection and a lower dosage of proton-pump inhibitors. The manometric findings showed no change throughout the time. A serious adverse event occurred, as one patient developed multiple cerebellar embolic infarcts. CONCLUSION: Because of the favorable effect in all patients, a safe route for esophageal fat injection needs to be developed.
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BACKGROUND: Based on an anatomical study of the perforators of the tensor fasciae latae (TFL) perforator flap, a clinical study was undertaken to confirm our data in a clinical trial and to demonstrate the ability to harvest a TFL perforator flap on septocutaneous perforators. METHODS: A retrospective case series analysis was performed of patients, who had undergone reconstruction of soft-tissue defects of the extremities, the groin and the head in 17 cases with a TFL perforator flap based on septocutaneous perforators; in three cases, a combined flap was used. The size of the flaps, the number of perforators, their external diameter, the length of the pedicle and the location and the distance from the anterior superior iliac spine (ASIS) were recorded. RESULTS: The average number of septocutaneous perforators per flap was 1.3 (range, 1-3); the average distance from the ASIS was 11 cm (range, 8-14 cm). The pedicle length varied between 4 and 10 cm, with an average of 7 cm; the average diameter of the pedicle was 4 mm (range, 1-5 mm). The average length of the flaps was 14 cm (range, 4.5-25), and the average width was 7 cm (range, 4.5-19). Donor site closure was achieved by direct closure in 14 patients, and in three patients with a split-thickness skin graft. In one case, at least two-thirds of the flap became necrotic because the septocutaneous perforator was located too far laterally from the flap centre. In one case of a combined flap, one skin island became partly necrotic due to compression after the pedicle was placed beneath the tendon of the extensor tibialis anterior muscle. One flap was successfully revised after venous thrombosis. CONCLUSIONS: The reliability and consistency of the septocutaneous perforators of the TFL flap make planning of this flap easy and the dissection straightforward. Although the number of complications is high in this series, only one complication is related to the flap and the planning itself. With the proposed modifications, we recommend this flap as an interesting alternative to other fasciocutaneous flaps.
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Fascia Lata/trasplante , Músculo Esquelético/trasplante , Procedimientos de Cirugía Plástica/métodos , Trasplante de Piel/métodos , Colgajos Quirúrgicos , Heridas y Lesiones/cirugía , Adolescente , Adulto , Anciano , Femenino , Estudios de Seguimiento , Ingle , Humanos , Masculino , Persona de Mediana Edad , Estudios Retrospectivos , Muslo , Adulto JovenRESUMEN
BACKGROUND: The purpose of this study was to differentiate between musculocutaneous and septocutaneous perforators of the tensor fasciae latae perforator flap; to evaluate their number, size, and location; and to provide landmarks to facilitate flap dissection. An additional injection study estimated the skin area of the flap. METHODS: The anatomical study was performed on 23 fixed and injected cadavers. The perforators of the tensor fasciae latae were identified and classified as septocutaneous or musculocutaneous. Diameter, location, and numbers were measured and the perforators were dissected up to their origin. The injection study was performed on 10 fresh cadavers. On one side, the ascending branch of the lateral circumflex femoral artery was injected with methylene blue; on the other side, the septocutaneous perforators were injected selectively. The size, location, and borders of the stained skin were measured. RESULTS: Forty-five thighs were included in this study. All perforators emerged from the ascending branch of the lateral circumflex artery. The average number of musculocutaneous perforators was 2.3 (range, 0 to 5), the distance from the anterior superior iliac spine was 10.9 cm (range, 4.5 to 16.1 cm), and the diameter was 0.9 mm (range, 0.2 to 2 mm). Four specimens had no musculocutaneous perforator. The average number of septocutaneous perforators was 1.8 (range, 1 to 3), the distance from the anterior superior iliac spine was 10.9 cm (range, 6.2 to 15.7 cm), and the diameter was 1.5 mm (range, 0.5 to 3 mm). Seventy-six percent of the septocutaneous perforators emerged between 8 and 12 cm from the anterior superior iliac spine. The possible pedicle length of a flap based on these vessels is 8.1 cm (range, 6.5 to 10 cm). In the injection study, the average skin area stained with methylene blue was 19.4 x 13.4 cm (range, 10 to 24 cm x 7 to 17 cm) in the ascending branch group. In the perforator group, the average skin area was 19.2 x 13.7 cm (range, 15 to 22 cm x 12 to 16 cm). CONCLUSIONS: The authors could show that the number of septocutaneous perforators for the tensor fasciae latae flap is more constant and that their diameter is greater than that of musculocutaneous perforators. The location of these perforators on a line extending from the ilium to the greater trochanter facilitates planning and dissection of a flap.