RESUMEN
BACKGROUND: A possible mechanism underlying cardiovascular morbidity after major vascular surgery may be the perioperative ischaemia-reperfusion with excessive oxygen-derived free-radical production and increased levels of circulating inflammatory mediators. We examined the effect of melatonin infusion during surgery and oral melatonin treatment for 3 days after surgery on biochemical markers of oxidative and inflammatory stress. METHODS: Patients received an intra-operative intravenous infusion of 50 mg melatonin or placebo. In addition, all patients received 10 mg melatonin or placebo orally the first 3 nights after surgery. Blood samples for analysis of malondialdehyde (MDA), ascorbic acid (AA), dehydroascorbic acid (DHA) and C-reactive protein (CRP) were collected preoperatively, and at 5 min, 6 h and 24 h after clamp removal (recirculation of the first leg). RESULTS: Twenty-six patients received melatonin and 24 patients received placebo. No significant differences were observed in any of the oxidative and inflammatory stress parameters. There were significantly more side effects in the melatonin group than in the placebo group. CONCLUSIONS: Melatonin treatment in the perioperative period did not reduce the oxidative and inflammatory parameters measured in this study.
Asunto(s)
Melatonina/farmacología , Estrés Oxidativo/efectos de los fármacos , Procedimientos Quirúrgicos Vasculares , Administración Oral , Adolescente , Adulto , Anciano , Anciano de 80 o más Años , Análisis de Varianza , Ácido Ascórbico/sangre , Biomarcadores/sangre , Proteína C-Reactiva/metabolismo , Distribución de Chi-Cuadrado , Cromatografía Líquida de Alta Presión , Ácido Deshidroascórbico/sangre , Método Doble Ciego , Femenino , Humanos , Inflamación/tratamiento farmacológico , Infusiones Intravenosas , Masculino , Malondialdehído/sangre , Melatonina/administración & dosificación , Persona de Mediana Edad , Placebos , Estadísticas no Paramétricas , Resultado del TratamientoRESUMEN
BACKGROUND: Melatonin, an endogenous circadian regulator, also has antioxidant and anti-inflammatory properties. The aim of this study was to evaluate the antioxidative effect of melatonin in patients undergoing laparoscopic cholecystectomy. METHODS: Patients were randomized to receive 10 mg melatonin or placebo during surgery. Blood samples for analysis of malondialdehyde (MDA), ascorbic acid (AA), total ascorbic acid (TAA) dehydroascorbic acid (DHA) and C-reactive protein (CRP) were collected pre-operatively and at 5 min, 6 h and 24 h after operation. RESULTS: Twenty patients received melatonin and 21 patients received placebo during surgery. No significant differences were observed between the groups in the oxidative stress variables MDA, TAA, AA and DHA or in the inflammatory variable CRP (repeated-measures ANOVA, P>0.05 for all variables). CONCLUSIONS: Administration of 10 mg melatonin did not reduce variables of oxidative stress in patients undergoing elective laparoscopic cholecystectomy.