RESUMEN
Glycosylphosphatidylinositol-anchored proteins can be specifically identified by several methods. PI-PLC digestion analyses, the most widely used technique, can be performed more reliably when conducted with purified protein and phase partitioning to exclude steric effects and when combined with alkaline hydrolysis to control for inositol acylation. Reductive radiomethylation not only can definitively identify a candidate protein as being GPI anchored, but also can provide information on the number of amine components (GlcN, ethanolamine) in the anchor structure. Biosynthetic labeling with anchor precursors is relatively specific when performed with [3H]ethanolamine or [3H]inositol. Incorporation of the precursors additionally can be used to (1) document anchor transfer to primary translation products, (2) identify soluble derivatives of GPI-anchored proteins that have been released from cell surfaces, and (3) localize the site of GPI anchor attachment within a GPI-anchored protein. A pathway for mammalian GP anchor assembly is depicted in Fig. 12. Initially GlcNAc is transferred to PI. The resulting GlcNAc-PI is then deacetylated to yield GlcN-PI. After that step, several points of divergence are identifiable between the mammalian and T. brucei pathways: (1) all mammalian Man-containing intermediates are built on acylated inositol phospholipids; (2) a proximal phosphoethanolamine is found in mammalian GPI anchor intermediates and is added to Man 1 prior to incorporation of Man 2 and Man 3; (3) no Gal branching substituent is added to the mammalian core glycan; and (4) the most polar mammalian GPI contains a third phosphoethanolamine substituent linked to the 6 position of Man 2.
Asunto(s)
Enzimas/metabolismo , Glicosilfosfatidilinositoles/metabolismo , Proteínas de la Membrana/metabolismo , Precursores de Proteínas/metabolismo , Acetilglucosamina/metabolismo , Animales , Autorradiografía/métodos , Bacterias/enzimología , Secuencia de Carbohidratos , Radioisótopos de Carbono , Electroforesis en Gel de Poliacrilamida/métodos , Precursores Enzimáticos/metabolismo , Enzimas/biosíntesis , Etanolamina , Etanolaminas/metabolismo , Glucolípidos/biosíntesis , Glucolípidos/química , Glucolípidos/metabolismo , Células HeLa , Humanos , Inositol/metabolismo , Mamíferos , Proteínas de la Membrana/biosíntesis , Datos de Secuencia Molecular , Fosfatidilinositol Diacilglicerol-Liasa , Fosfoinositido Fosfolipasa C , Hidrolasas Diéster Fosfóricas/metabolismo , Polisacáridos/química , Técnica de Dilución de Radioisótopos , Radioisótopos de Azufre , TritioRESUMEN
The genetic lesion in paroxysmal nocturnal hemoglobinuria (PNH) cells resides in a DNA element that 1) encodes a product required for assembly of GlcNAc-inositol phospholipid and 2) is commonly affected in different patients. In this study, three alternative mRNA transcripts (1600, 1200, and 950 bp) that derive from this genetic element in normal cells were characterized. The 1200-bp transcript was found to arise from splicing out of 374 bp of exonic sequence extending from positions 407-780. The 950-bp transcript was found to arise from removal of this and 284 bp of additional exonic sequence beginning further upstream at position 123. Analyses of transcripts expressed in Epstein-Barr virus (EBV)-transformed B lymphocytes prepared from two PNH patients showed that both failed to express normal 1600-bp transcripts. One expressed truncated transcripts of 1000 and 800 bp generated by an alternate splice which utilized a downstream signal in place of the normal intronic splice signal. The other expressed a 1600-bp transcript with multiple nucleotide changes but normal 1200- and 950-bp "spliced" transcripts.
Asunto(s)
Glicosilfosfatidilinositoles/genética , Hemoglobinuria Paroxística/genética , Proteínas de la Membrana/genética , Empalme Alternativo , Secuencia de Aminoácidos , Antígenos CD/genética , Antígenos CD/inmunología , Antígenos CD/metabolismo , Eliminación de Gen , Glicosilfosfatidilinositoles/metabolismo , Hemoglobinuria Paroxística/metabolismo , Humanos , Linfoma/genética , Linfoma/inmunología , Linfoma/metabolismo , Proteínas de la Membrana/metabolismo , Datos de Secuencia Molecular , Células Tumorales CultivadasRESUMEN
Although the insulin-dependent hydrolysis of glycosyl-phosphatidylinositol (GPI) may play an important role in insulin action, an absolute requirement for this glycolipid has not been demonstrated. Human K562 cells were mutated to produce a cell line (IA) incapable of the earliest step in PI glycosylation, the formation of PI-GlcNAc. Another cell line (IVD) was deficient in the deacetylation of PI-GlcNAc to form PI-GlcN and subsequent mannosylated species. Each line was transfected with wild-type human insulin receptors. Similar insulin-stimulated receptor autophosphorylation was observed in all three lines, along with a nearly identical increase in the association of phosphorylated insulin receptor substrate 1 with endogenous PI 3-kinase. Both normal and GPI-defective lines also displayed a similar 2- to 3-fold increase in phosphorylation of the Shc protein and its association with growth factor receptor-bound protein 2 in response to insulin. In contrast to these results, striking differences were noted in insulin-stimulated glycogen synthesis. In normal cells, glycogen synthesis was significantly increased by insulin, whereas no insulin stimulation was observed in GPI-deficient IA cells, and only a trace of stimulation was detected in IVD cells. These results indicate that tyrosine phosphorylation produced by insulin is not dependent on GPI synthesis, and this effect is not sufficient to elicit at least some of the metabolic effects of the hormone. In contrast, GPI synthesis is required for the stimulation of glycogen synthesis by insulin in these cells. These findings support the existence of divergent pathways in the action of insulin.
Asunto(s)
Glucógeno/biosíntesis , Glicosilfosfatidilinositoles/biosíntesis , Insulina/farmacología , Leucemia Eritroblástica Aguda/metabolismo , Receptor de Insulina/fisiología , Línea Celular , Citometría de Flujo , Glucosa/metabolismo , Humanos , Cinética , Mutagénesis , Fosforilación , Fosfotirosina , Receptor de Insulina/biosíntesis , Receptor de Insulina/aislamiento & purificación , Proteínas Recombinantes de Fusión/biosíntesis , Proteínas Recombinantes/biosíntesis , Proteínas Recombinantes/aislamiento & purificación , Proteínas Recombinantes/metabolismo , Transfección , Células Tumorales Cultivadas , Tirosina/análogos & derivados , Tirosina/análisisRESUMEN
The survival of transfused red cells (RBCs) diminishes with time of in vitro storage in blood banks, but the molecular mechanisms underlying the slow but incessant deterioration are incompletely understood. To investigate the possibility that impaired resistance to autologous complement attack could play a role in this phenomenon, packed RBCs stored for variable periods were assayed for decay-accelerating factor (DAF) and CD59, two glycoinositol-phospholipid (GPI)-anchored, membrane-associated complement regulatory proteins that function physiologically to protect blood cells from autologous complement activation on their surfaces. Immunoradiometric and flow cytometric assays employing DAF and CD59 monoclonal antibodies showed that levels of both surface proteins gradually declined over 6 weeks. Digestion analyses with phosphatidylinositol-specific phospholipase C, an enzyme that releases GPI-anchored proteins from cell surfaces, showed that DAF and CD59 molecules with GPI anchors containing unacylated inositol were preferentially lost. These findings suggest: 1) that DAF and CD59 molecules with acylated GPI anchors are more stable in RBC membranes than are molecules with unacylated GPI anchors, and 2) that DAF and CD59 loss may participate with other membrane alterations that occur during in vitro storage in compromising the survival of transfused cells.
Asunto(s)
Antígenos CD/análisis , Donantes de Sangre , Conservación de la Sangre , Proteínas Inactivadoras de Complemento/análisis , Eritrocitos , Glicoproteínas de Membrana/análisis , Bancos de Sangre , Antígenos CD55 , Antígenos CD59 , Eritrocitos/química , Eritrocitos/inmunología , Citometría de Flujo , Fluorescencia , Glicosilfosfatidilinositoles/metabolismo , Glicosilfosfatidilinositoles/farmacología , Humanos , Factores de TiempoRESUMEN
Every protein fated to receive the glycophosphatidylinositol (GPI) anchor post-translational modification has a C-terminal GPI-anchor attachment signal sequence. This signal peptide varies with respect to length, content, and hydrophobicity. With the exception of predictions based on an upstream amino acid triplet termed omega-->omega + 2 which designates the site of GPI uptake, there is no information on how the efficiencies of different native signal sequences compare in the transamidation reaction that catalyzes the substitution of the GPI anchor for the C-terminal peptide. In this study we utilized the placental alkaline phosphatase (PLAP) minigene, miniPLAP, and replaced its native 3' end-sequence encoding omega-2 to the C-terminus with the corresponding C-terminal sequences of nine other human GPI-anchored proteins. The resulting chimeras then were fed into an in vitro processing microsomal system where the cleavages leading to mature product from the nascent preproprotein could be followed by resolution on an SDS-PAGE system after immunoprecipitation. The results showed that the native signal of each protein differed markedly with respect to transamidation efficiency, with the signals of three proteins out-performing the others in GPI-anchor addition and those of two proteins being poorer substrates for the GPI transamidase. The data additionally indicated that the hierarchical order of efficiency of transamidation did not depend solely on the combination of permissible residues at omega-->omega + 2.
Asunto(s)
Fosfatasa Alcalina/metabolismo , Glicosilfosfatidilinositoles/metabolismo , Proteínas Gestacionales/metabolismo , Precursores de Proteínas/metabolismo , Procesamiento Proteico-Postraduccional/fisiología , Señales de Clasificación de Proteína/fisiología , Fosfatasa Alcalina/genética , Glicosilfosfatidilinositoles/genética , Células HeLa/metabolismo , Humanos , Células K562/citología , Células K562/metabolismo , Leucemia Eritroblástica Aguda/metabolismo , Microsomas/metabolismo , Mutagénesis/genética , Mutagénesis/fisiología , Proteínas Gestacionales/genética , Ingeniería de Proteínas/métodos , Proteínas Recombinantes de Fusión/genética , Proteínas Recombinantes de Fusión/metabolismoRESUMEN
The final step in glycosylphosphatidylinositol (GPI) anchoring of cell surface proteins consists of a transamidation reaction in which preassembled GPI donors are substituted for C-terminal signal sequences in nascent polypeptides. In previous studies we described a human K562 cell mutant, termed class K, that accumulates fully assembled GPI units but is unable to transfer them to N-terminally processed proproteins. In further work we showed that, unlike wild-type microsomes, microsomes from these cells are unable to support C-terminal interaction of proproteins with the small nucleophiles hydrazine or hydroxylamine, and that the cells thus are defective in transamidation. In this study, using a modified recombinant vaccinia transient transfection system in conjunction with a composite cDNA prepared by 5' extension of an existing GenBank sequence, we found that the genetic element affected in these cells corresponds to the human homolog of yGPI8, a gene affected in a yeast mutant strain exhibiting similar accumulation of GPI donors without transfer. hGPI8 gives rise to mRNAs of 1.6 and 1.9 kb, both encoding a protein of 395 amino acids that varies in cells with their ability to couple GPIs to proteins. The gene spans approximately 25 kb of DNA on chromosome 1. Reconstitution of class K cells with hGPI8 abolishes their accumulation of GPI precursors and restores C-terminal processing of GPI-anchored proteins. Also, hGPI8 restores the ability of microsomes from the mutant cells to yield an active carbonyl in the presence of a proprotein which is considered to be an intermediate in catalysis by a transamidase.
Asunto(s)
Aciltransferasas/genética , Regulación de la Expresión Génica , Glicosilfosfatidilinositoles/genética , Secuencia de Bases , Línea Celular , Clonación Molecular , Técnicas de Transferencia de Gen , Humanos , Datos de Secuencia Molecular , Mutación , Saccharomyces cerevisiae , Alineación de SecuenciaRESUMEN
CD59, the membrane regulator of autologous C5b-9 channel formation, exhibits variable sensitivity to cleavage by phosphatidylinositol-specific phospholipase C (PI-PLC), an enzyme that releases glyco-inositolphospholipid (GPI)-anchored proteins from cell surfaces. To determine whether the GPI-anchor phospholipid of CD59 is similar to that of decay-accelerating factor (DAF) and whether variation in its structure underlies its variable enzyme susceptibility, the GPI anchors of the two proteins expressed on erythrocytes, polymorphonuclear and mononuclear leucocytes were compared in situ and after purification. Flow cytometric analyses of PI-PLC-treated cells showed parallel cell type specific release of both proteins as a function of enzyme concentration. Non-denaturing PAGE analyses of alkaline/hydroxylamine-treated proteins (affinity-purified from [125I]-surface-labelled cells) provided evidence for (i) comparable proportions of GPI-anchor acylation, and (ii) alkali-resistant rather than alkali-sensitive lipid substituents in erythrocytes. These findings argue that the differential C5b-9 sensitivity that distinguishes paroxysmal nocturnal haemoglobinuria II and III erythrocytes does not derive from expression of CD59 molecules with alternative GPI-anchor phospholipid structures.
Asunto(s)
Antígenos CD/química , Complejo de Ataque a Membrana del Sistema Complemento/antagonistas & inhibidores , Glucolípidos/química , Glicoproteínas de Membrana/química , Fosfatidilinositoles/química , Proteínas Sanguíneas/química , Antígenos CD55 , Antígenos CD59 , Proteínas Inactivadoras de Complemento/química , Electroforesis en Gel de Poliacrilamida , Eritrocitos/efectos de los fármacos , Citometría de Flujo , Glicosilfosfatidilinositoles , Humanos , Leucocitos Mononucleares/efectos de los fármacos , Proteínas de la Membrana/química , Neutrófilos/efectos de los fármacos , Relación Estructura-Actividad , Fosfolipasas de Tipo C/farmacologíaRESUMEN
Deficient expression of glycoinositol phospholipid (GPI)-anchored surface proteins has been linked to six different genetic defects in Thy-1- murine lymphoma mutants. In this study, human K562 cell mutants defective in GPI anchoring were derived by anti-decay-accelerating factor (CD55) based negative fluorescent cell sorting of N-methyl-N'-nitro-N-nitrosoguanidine pretreated cells. Homologous cell fusions of six clones that complemented a previously described K562 mutant corresponding to one of the Thy-1- mutant classes (Hirose, S., Mohney, R. P., Mutka, S. C., Ravi, L., Singleton, D. R., Perry, G., Tartakoff, A., and Medof, M. E. (1992) J. Biol. Chem. 267, 5272-5278) showed that they segregated into two complementation groups. In heterologous cell fusions, representative clones of each group complemented Thy-1 expression by all of the previously described GPI anchor pathway-defective Thy-1- murine lymphoma classes (A, B, C, E, F, and H) but not class(es) D (and I) defective in the Thy-1 structural gene. Analyses of putative GPI anchor precursors synthesized by the two lines revealed that one mutant exhibited a complete block in deacetylation of N-acetyl-D-glucosamine-inositol phospholipid to glucosamine (GlcN)-inositol phospholipid, whereas the other mutant assembled GlcN-inositol phospholipid and subsequent mannose (Man)-containing intermediates but showed markedly increased amounts of the terminal ethanolamine (EthN)-phosphate (P)-substituted putative anchor precursors, EthN-P-6ManMan(EthN-P-->)ManGlcN- and EthN-P-6Man(EthN-P-6)Man(EthN- P-->)ManGlcN-acylinositol phospholipid (H7 and H8). We designate these new complementation classes J, harboring a defect in N-acetyl-D-glucosamine-inositol phospholipid deacetylation, and K, deficient in a step preliminary to or associated with protein transfer of assembled anchor precursors. The availability of these new mutant classes should aid in characterization of the GPI anchor pathway enzymes providing for these reactions.
Asunto(s)
Antígenos de Superficie/genética , Glicosilfosfatidilinositoles/biosíntesis , Linfoma/genética , Glicoproteínas de Membrana/genética , Animales , Antígenos de Superficie/biosíntesis , Secuencia de Carbohidratos , Línea Celular , Células Clonales , Citometría de Flujo , Glicosilfosfatidilinositoles/genética , Humanos , Leucemia Mielógena Crónica BCR-ABL Positiva , Glicoproteínas de Membrana/biosíntesis , Metilnitronitrosoguanidina/toxicidad , Ratones , Datos de Secuencia Molecular , Mutagénesis , Fosfatidilinositoles/biosíntesis , Antígenos Thy-1 , Células Tumorales Cultivadas , Uridina Difosfato Glucosa/metabolismo , Uridina Difosfato N-Acetilglucosamina/metabolismoRESUMEN
Deficient expression of glycoinositol phospholipid (GPI) anchored proteins in affected paroxysmal nocturnal hemoglobinuria (PNH) cells has been traced to a defect in GPI anchor assembly. In a previous study (Schubert, J., Schmidt, R. E., and Medof, M. E. (1993) J. Biol. Chem., in press) we characterized the biosynthesis of putative Man-containing GPI anchor precursors in normal peripheral blood lymphocytes and investigated assembly of these intracellular GPI intermediates in CD48- affected and CD48+ unaffected T and natural killer cell lines of PNH patients. We found that affected T cells from five patients exhibited a uniform defect in which dolichol-phosphoryl-Man was synthesized but no GPI mannolipids were expressed. In this study, membranes of patients' affected T cells were labeled with UDP-[3H]GlcNAc to evaluate earlier steps in GPI synthesis, and intact cells were fused to Thy-1- murine lymphoma mutants harboring different defects in early GPI assembly to test for the presence of corresponding or complementary lesions. In all cases, affected cell membranes failed to assemble GlcNAc-inositol phospholipid, the initial precursor of GPI anchor structures, and the intact cells failed to complement class A mutants while complementing other classes. Affected polymorphonuclear leukocytes from three additional patients of different origin were then labeled with [3H]Man and the labeling patterns found to correspond to those obtained with the T lymphocytes. Taken together the data indicate that the genetic lesion in PNH cells resides in a DNA element which: 1) encodes a product required for the synthesis of GlcNAc-inositol phospholipid, 2) corresponds to that altered in class A Thy-1- murine lymphoma mutants, and 3) is commonly affected in different patients.
Asunto(s)
Acetilglucosamina/metabolismo , Glicosilfosfatidilinositoles/metabolismo , Hemoglobinuria Paroxística/inmunología , Hemoglobinuria Paroxística/metabolismo , Linfoma/inmunología , Linfoma/metabolismo , Animales , Antígenos CD/genética , Antígeno CD48 , Membrana Celular/metabolismo , Monofosfato de Dolicol Manosa/metabolismo , Hemoglobinuria Paroxística/genética , Humanos , Células Asesinas Naturales/inmunología , Linfoma/genética , Manosa/sangre , Ratones , Mutación , Neutrófilos/inmunología , Neutrófilos/metabolismo , Trypanosoma brucei brucei/metabolismo , Uridina Difosfato N-Acetilglucosamina/metabolismoRESUMEN
The genetic lesion in paroxysmal nocturnal hemoglobinuria (PNH) cells resides in a DNA element that 1) encodes a product required for assembly of GlcNAc-inositol phospholipid and 2) is commonly affected in different patients. In this study, three alternative mRNA transcripts (1600, 1200 and 950 bp) that derive from this genetic element in normal cells were characterized. The 1200-bp transcript was found to arise from splicing out of 374 bp of exonic sequence extending from positions 407-780. The 950-bp transcript was found to arise from removal of this and 284 bp of additional exonic sequence beginning further upstream at position 123. Analyses of transcripts expressed in Epstein-Barr virus (EBV)-transformed B lymphocytes prepared from two PNH patients showed that both failed to express normal 1600-bp transcripts. One expressed truncated transcripts of 1000 and 800 bp generated by an alternate splice which utilized a downstream signal in place of the normal intronic splice signal. The other expressed a 1600 bp-transcript with multiple nucleotide changes but normal 1200- and 950- bp "spliced" transcripts