Global spread, genetic differentiation and selection of barley spot form of net blotch isolates.

Spot form of net blotch, caused by Pyrenophora teres f. maculata, is a significant necrotrophic disease of barley that spread world-wide in the 20th century. Genetic relationships were analysed to determine the diversity, survival and dispersal of a diverse collection of 346 isolates from Australia, Southern Africa, North America, Asia Minor and Europe. The results, based on genome-wide DArTseq data, indicated isolates from Turkey were the most differentiated with regional sub-structuring, together with individuals closely related to geographically distant genotypes. Elsewhere, population subdivision related to country of origin was evident, although low levels of admixturing was found that may represent rare genotypes or migration from unsampled populations. Canadian isolates were the next most diverged and Australian and South African the most closely related. With the exception of Turkish isolates, multiple independent Cyp51A mutation events (which confer insensitivity to demethylation inhibitor fungicides) between countries and within regions was evident, with strong selection for a transposable element insertion at the 3' end of the promoter and counter-selection elsewhere. Individuals from Western Australia shared genomic regions and Cyp51A haplotypes with South African isolates, suggesting a recent common origin.

Growth patterns and outcomes of growth hormone therapy in patients with acrodysostosis.

INTRODUCTION: Severe short stature is a feature of acrodysostosis, but data on growth are sparse. Treatment with recombinant human growth hormone (rhGH) is used in some centers to increase final height, but no studies have been published so far. Our objective was to conduct a multicenter, retrospective, cohort study to investigate growth in individuals with both types of acrodysostosis, treated with rhGH or not; we used the new nomenclature to describe acrodysostosis, as this disease belongs to the large group of inactivating PTH/PTHrP signaling disorders (iPPSD); acrodysostosis refers to iPPSD4 (acrodysostosis type 1 due to PRKAR1A mutations) and iPPSD5 (acrodysostosis type 2, due to PDE4D mutations). METHODS: We present auxological data from individuals with genetically characterized iPPSD4, and participants with clinical features of iPPSD5. RESULTS: We included 20 and 17 individuals with iPPSD4 and iPPSD5, respectively. The rhGH-treated iPPSD4 patients (n = 9) were smaller at birth than those who did not receive rhGH (median - 2.2 SDS vs. - 1.7 SDS); they showed a trend to catch-up growth during rhGH therapy (median 0.5 SDS in the first year). The rhGH-treated patients (n = 5) reached a better final height compared to those who did not receive rhGH (n = 4) (median - 2.8 SDS vs. - 3.9 SDS), suggesting that rhGH is efficient to increase height in those patients. The difference in target height to final height ranged between 1.6 and 3.0 SDS for iPPSD4 not treated with rhGH (n = 4), 2.1-2.8 SDS for rhGH-treated iPPSD4 (n = 5), 0.6-5.5 SDS for iPPSD5 not treated with rhGH (n = 5) and 2.5-3.1 for rhGH-treated iPPSD5 (n = 2). CONCLUSION: Final height may be positively influenced by rhGH in patients with acrodysostosis/iPPSD. Our rhGH-treated cohort started therapy relatively late, which might explain, at least in part, the limited effect of rhGH on height.

Retrospective and Contemporary Predictors of National Collegiate Athletic Association Division I Cross-Country Performance Are Sex Specific.

ABSTRACT: Carder, MJ, Scudamore, EM, Savanna, KN, Pribyslavska, V, Bowling, LR, and O'Neal, EK. Retrospective and contemporary predictors of National Collegiate Athletic Association Division I cross-country performance are sex specific. J Strength Cond Res 37(11): 2267-2272, 2023-The purpose of this study was to identify National Collegiate Athletic Association (NCAA) Division I cross-country (XC) performance potential using laboratory-based and field-based parameters and retrospective high school (HS) personal best (PB) data at various distances of current collegiate XC runners. Fifteen female and 17 male NCAA XC runners provided their PB for 5-km (women) and 8-km (men) distances from the previous season. Bivariate correlation and stepwise and hierarchical regression modeling were used to predict XC performance. Single squat jump height and multijump reactive strength index displayed r < 0.27 for both sexes, suggesting lower-body power is a poor predictor of XC performance or masked by other factors of greater importance. Triceps skinfold thickness approached significance (r = 0.43; p = 0.09) for men but was unrelated to women's performance (r = -0.05; p = 0.86). HS XC PB neared significance (r = 0.55, p = 0.054), but no other single or combination of variables reached significance for female runners. Aerobic capacity displayed a moderate to strong relationship (r = 0.65) for male runners. High school 3,200-m PB for men produced a robust prediction capacity (r = 0.85; p = 0.005, SEE = ± 0.65 minutes), and predicted 8-km PB within 30 seconds for approximately two-thirds of runners. These outcomes suggest when recruiting HS or transfer athletes, male and female XC runners should not be recruited on the same factors. Women's XC PB is more difficult to predict, but skinfold thickness was statistically the least valuable predictor of all factors.

Strategies for the removal of halides from drinking water sources, and their applicability in disinfection by-product minimisation: a critical review.

The presence of bromide (Br(-)) and iodide (I(-)) in source waters leads to the formation of brominated and iodinated disinfection by-products (DBPs), which are often more toxic than their chlorinated analogues. The increasing scarcity of water resources in Australia is leading to use of impaired and alternative water supplies with high bromide and iodide levels, which may result in the production of more brominated and iodinated DBPs. This review aims to provide a summary of research into bromide and iodide removal from drinking water sources. Bromide and iodide removal techniques have been broadly classified into three categories, namely; membrane, electrochemical and adsorptive techniques. Reverse osmosis, nanofiltration and electrodialysis membrane techniques are reviewed. The electrochemical techniques discussed are electrolysis, capacitive deionization and membrane capacitive deionization. Studies on bromide and iodide removal using adsorptive techniques including; layered double hydroxides, impregnated activated carbons, carbon aerogels, ion exchange resins, aluminium coagulation and soils are also assessed. Halide removal techniques have been compared, and areas for future research have been identified.

Superheated ice: true compression fractures and fast internal melting.

Internal melt figures can be nucleated in ice without the presence of a vapor bubble. Their form and growth are fracture-like, different from the normal Tyndall stars, which do contain vapor bubbles. Normal Tyndall figures that grow rapidly are not oriented in the basal plane, and very rapid internal melting gives a peculiar, systematic growth of clouds of Tyndall figures.

Severe disruptive vocalizers.

OBJECTIVES: To describe the most severe disruptive vocalizers in nursing facilities, in regard to their clinical and behavioral characteristics, staff responses, and treatments used, and to report on their prognosis over 6 months. DESIGN: A longitudinal cohort study. SETTING: One hundred seven skilled nursing facilities. PARTICIPANTS: The 203 residents who were among the two most disruptive vocalizers in their respective facilities and who vocalized at least 2 hours a day. MEASUREMENTS: Telephone interviews of licensed nursing staff who cared for the subjects, conducted at baseline, 2, 4, and 6 months. Data gathered included subject demographics, physical function, diagnoses, medication and restraint use, behavioral problems, vocalization characteristics, treatments used, and status at follow-up. RESULTS: Subjects tended to have dementia, to be dependent in most activities of daily living, to have multiple medical problems, to be physically restrained (48%), and to be taking psychotropic medication (76%). Nearly all (95%) were audible at least 50 feet away, with loudness associated with more severe cognitive impairment (OR 4.90, P = .001). When subjects who primarily made nonverbal noises ("screamers") were compared with those whose predominant expressions were words ("talkers"), hearing impairment, severe cognitive impairment, and greater dependency in activities of daily living characterized the screamers. Staff reported trying a variety of treatments with all subjects, often with little success. Two months after enrollment, 66% of surviving subjects vocalized fewer hours than at baseline, and 45% were rated as improved. Independent predictors of improvement included greater ADL independence, hearing and vision problems, shorter length of stay, urinary incontinence, and use of a treatment other than one-on-one interventions. Nearly one-quarter of subjects (23.4%) died within 6 months. Baseline factors associated independently with a higher probability of death included age, use of activity intervention, physical abusiveness, and absence of wandering. CONCLUSION: Severe disruptive vocalization is associated with severe cognitive and physical impairment and with a high probability of mortality within 6 months. Subcategorization of severe vocalizers by vocalization type or other associated factors may be useful for prognostic and treatment purposes.

A case of myoepithelial carcinoma displaying biallelic inactivation of the tumour suppressor gene APC in a patient with familial adenomatous polyposis.

Familial adenomatous polyposis (FAP) is an autosomal dominant disorder caused by mutation of the APC gene. It is characterised by the appearance of hundreds to thousands of colorectal adenomas in adolescence and the subsequent development of colorectal cancer. Various extracolonic malignancies are associated with FAP, including desmoids and neoplasms of the stomach, duodenum, pancreas, liver, and brain. We present a family affected by FAP with an exon 14 APC mutation displaying two rare extracolonic lesions, a hepatoblastoma and a myoepithelial carcinoma. The hepatoblastoma was found in a male patient aged 2 years. The second lesion, a myoepithelial carcinoma of the right cheek, was found in a female patient aged 14 years. Inactivation of the normal APC allele was demonstrated in this lesion by loss of heterozygosity analysis, thus implicating APC in the initiation or progression of this neoplasm. This is the first reported case of this lesion in a family affected by FAP.

An evaluation of new gel matrices for the separation of PCR-amplified DNA fragments.

It has been shown that the novel gel matrix, PCR Purity Plus which consists of a vinyl-polymer of polyacrylamide, provides a superior and rapid means of separating DNA fragments. As this product has been discontinued, the two new commercial versions of this matrix (MDE and GeneAmp) with PCR Purity Plus were compared. Optimal conditions for resolving DNA fingerprint profiles for both matrices were defined. Both MDE and GeneAmp gels provided a clear separation of DNA fragments. However, the profiles obtained on GeneAmp gel were closest to that of PCR Purity Plus. These results should be useful to DNA fingerprinting studies where it is critical to obtain a clear resolution of complex DNA profiles.

Computer image analysis of pigmented skin lesions.

To assist in the distinction of melanoma from benign pigmented lesions, an imaging system was developed, comprising a frame grabber, a microcomputer, a colour video camera and flash lighting with red, green and infrared filters. Over an 18-month period, video images of 70 unselected pigmented lesions for which complete diagnostic data were available, were successfully captured using the camera. Analysis software extracted features relevant to the size, colour, shape and boundary of each lesion, and these features were correlated with clinical and histological characteristics on which standard diagnoses of skin tumours are based. For discriminant analysis based on image analysis measurements, equal probabilities were assigned to three specified diagnostic groups, namely melanoma, naevi and 'other', and four of five melanomas were correctly classified when infrared data were included. However when infrared measurements were omitted, all five melanomas were correctly classified, and the overall accuracy of classification of pigmented lesions was 71%. This system holds promise as an aid in the clinical distinction of melanoma from benign pigmented skin lesions.

Hypersensitive dentin: testing of procedures for mechanical and chemical obliteration of dentinal tubuli.

This study examined by scanning electron microscopy the effect of various mechanical and chemical procedures in obliterating dentinal tubuli. Dentin blocks containing open, cross-sectioned dentinal tubuli were separated into one experimental and one control area. The mechanical treatments consisted of 20 seconds of continuous instrumentation with sharp and dull curets, finely textured inserts for the EVA reciprocating handpiece, metal and plastic inserts for a sonic scaler, and metal inserts for an ultrasonic scaler. Chemical treatments included a light-cured dental resin and active obliterating agents including sodium, stannous and hydrogen fluorides, potassium oxalate, glycerin, ferric oxalate, and potassium nitrate. These substances were applied to the test surfaces for 2 minutes and allowed to dry. One half of the chemically treated specimens were then sprayed with water in order to evaluate the retention of the obliterating agents. The results revealed that among the mechanical treatments, the sharp curet gave the most consistent and complete obliteration of the tubuli. The plastic inserts had no tubular occluding effects. Among the chemical procedures, the light-cured resin resulted in the most complete obliteration of the tubuli, while the fluoride agents and glycerin had no effect. It can be hypothesized that combining instrumentation with a sharp curet causing a heavy smear layer and occlusion of tubuli apertures, followed by application of a light body resin, may prove to be a rational method of desensitizing hypersensitive dentin surfaces. However, this combined treatment should be evaluated in clinical trials.

Construction of shoulder and breast prostheses.

The usual surgical treatment for patients who have osteogenic sarcoma of the humerus is forequarter amputation. Hence, a prosthetic shoulder is needed in order to compensate for the resulting deformity. Occasionally, female patients must also undergo a radical mastectomy and as a result require a comination breast-shoulder prosthesis. The occupational therapy staff at M.D. Anderson Hospital and Tumor Institute devised a procedure for the construction of shoulder and/or breast prostheses. Methods of fabrication and problems encountered are discussed. As medical treatments improve and survival times are lengthened, more cancer patients will require rehabilitation services. This article may provide assistance for therapists who are faced with similar problems in the rehabilitative process.

Chlorine disinfection by-products in wastewater effluent: Bioassay-based assessment of toxicological impact.

The potential ecological impact of disinfection by-products (DBPs) present in chlorinated wastewater effluents is not well understood. In this study, the chlorinated effluent of traditional wastewater treatment plants (WWTPs) and advanced water reclamation plants (AWRPs) supplying highly-treated recycled water were analyzed for nitrosamines and trihalomethanes (THMs), and a battery of bioassays conducted to assess effluent toxicity. An increase in general toxicity from DBPs was revealed for all wastewaters studied using an in vitro bioluminescence assay. Examples of androgenic activity and estrogenic activity arising from DBPs at specific sampling sites were also observed. The in vivo model (Artemia franciscana) was generally not adversely affected by exposure to DBPs from any of the chlorinated wastewaters studied. The observed toxicity could not be related to the concentrations of THMs and nitrosamines present, indicating that DBPs not monitored in this study were responsible for this. This work highlights the complexity of DBPs mixtures formed in chlorinated wastewaters, illustrating that toxicity of wastewater DBPs cannot be predicted by chemical monitoring of THMs and nitrosamines. The results suggest bioassays may be particularly useful monitoring tools in assessing toxicity arising from DBPs of these complex waters. The research concludes that DBPs formed in the chlorinated wastewaters studied can be toxic and may have a deleterious impact on aquatic organisms that are exposed to them, and therefore, that chlorination or chlorination/dechlorination may not be adequate treatment strategies for the protection of receiving waters. Chlorinated wastewater toxicity (from DBPs) is not well-understood in the Australian context, and this study serves to advise regulators on this issue.

Seventy-five years of the RSNA (Radiological Society of North America), approaching a century of radiology: museum and information resources.

As the Centenary approaches, the feel of radiology history is changing. As exhibits are mounted and the sub-specialties tell their own stories, what once seemed a very new field is acquiring a codified history of its own. That story is made up, to a very large extent, by the things and memories we happen to have kept. As we move into a second century of radiology, the question of what to save and how to save it becomes even more challenging.

Severe sensorineural deafness in children due to perforation of the round-window membrane.

Perforation of the round-window membrane was found in three children with severe sensorineural deafness. After a simple repair, all three have had a small improvement in hearing.

Round window membrane rupture and acquired sensorineural hearing loss in children.

The aetiology of acquired sensorineural hearing loss includes many conditions. The disparity between the number of children affected by the conditions which are supposed to cause hearing loss and the number of children who are actually deaf, has never been satisfactorily explained. Clinical features observed in children with surgically proven round window membrane rupture have been noted in all conventional aetiological groups. These features include otitis media, doubt about the hearing status in early life, disturbance of balance and other occasional neurological phenomena. Wide variation in the appearance of round window niche has been observed in children with secretory otitis and similar appearances have been observed in children with proven round window membrane rupture. The appearances may represent a reaction to perilymph. Round window membrane rupture is probably common and may prove to be a unifying factor for many cases of acquired sensorineural hearing loss. Occasional neurological phenomena may be related to it.

Applicability of aeration and delayed addition of selenite to the isolation of salmonellae.

The effect of aeration combined with the delayed addition of selenite on the lag period of several strains of salmonellae and other enterobacteria is reported. A procedure has been developed involving shaking of the sample in a basal medium for 4 hr at 37 C, adding selenite and cystine, and continuing shaking for 20 hr. Confirmation by selective plating, biochemical tests, and serology gave results comparable to the standard lactose pre-enrichment method with the saving of 24 hr and elimination of one set of media. Confirmation by fluorescent-antibody tests showed that fewer positive fluorescent stains were obtained from the aerated procedure than from the lactose pre-enrichment procedure. Precautions in the application of this procedure are discussed.