Optimized radiofrequency coil setup for MR examination of living isolated rat hearts in a horizontal 9.4T magnet.

PURPOSE: (i) To optimize an MR-compatible organ perfusion setup for the nondestructive investigation of isolated rat hearts by placing the radiofrequency (RF) coil inside the perfusion chamber; (ii) to characterize the benefit of this system for diffusion tensor imaging and proton ((1) H-) MR spectroscopy. METHODS: Coil quality assessment was conducted both on the bench, and in the magnet. The benefit of the new RF-coil was quantified by measuring signal-to-noise ratio (SNR), accuracy, and precision of diffusion tensor imaging/error in metabolite amplitude estimation, and compared to an RF-coil placed externally to the perfusion chamber. RESULTS: The new design provided a 59% gain in signal-to-noise ratio on a fixed rat heart compared to using an external resonator, which found reflection in an improvement of living heart data quality, compared to previous external resonator studies. This resulted in 14-29% improvement in accuracy and precision of diffusion tensor imaging. The Cramer-Rao lower bounds for metabolite amplitude estimations were up to 5-fold smaller. CONCLUSION: Optimization of MR-compatible perfusion equipment advances the study of rat hearts with improved signal-to-noise ratio performance, and thus improved accuracy/precision.

Optically detunable, inductively coupled coil for self-gating in small animal magnetic resonance imaging.

An inductively coupled coil concept is presented, which improves the compensation of physiological motion by the self-gating (SG) technique. The animal is positioned in a conventional volume coil encompassing the whole animal. A small, resonant surface coil (SG-coil) is placed on the thorax so that its sensitive region includes the heart. Via inductive coupling the SG-coil amplifies selectively the MR signal of the beating heart. With an optical detuning mechanism, this coupling can be switched off during acquisition of the MR image information, whereas it is active during SG data sampling to provide the physiological information. In vivo experiments on a mouse show an amplification of the SG signal by at least 40%.

An expandable catheter loop coil for intravascular MRI in larger blood vessels.

The present study proposes a catheter system with an expandable coil etched on a polyimide foil. The catheter system combines the advantages of a small insertion diameter when the coil is rolled up in a protective carrier sheath with an increased signal-to-noise ratio (SNR) and penetration depth when the coil is pushed out. After imaging, the coil can be retracted into the sheath and folded back into the initial rolled-up configuration due to the tapered geometry of the carrier foil. The catheter system was tested on two healthy anesthetized pigs, including tracking and high-resolution intravascular imaging. To reduce artifacts in high-resolution images induced by catheter motion in the pulsatile blood flow, a motion-gating method was implemented that combines a flow-compensated two-dimensional fast low angle shot (FLASH) imaging sequence with the acquisition of projection data for retrospective gating. Using the projection data for motion detection, image SNR was increased by up to 500% over uncorrected images, and anatomic structures of 150 microm size could be differentiated in the aorta.

Measurement of R1 dynamics using sliding window-DESPOT.

PURPOSE: To measure longitudinal relaxation rate (R1) changes during contrast agent studies using a driven equilibrium single pulse observation of T1 (DESPOT) method with a sliding window (sw) acquisition. MATERIALS AND METHODS: A sw-DESPOT technique was implemented that uses several three-dimensional (3D) image data sets to calculate R1 with a temporal resolution of only a single data set. Different sources of systematic errors were studied in simulations, and the technique was tested in a tumor-bearing mouse using an intravascular contrast agent. RESULTS: Consistent concentration distributions of the CA were calculated with a temporal resolution of 10 s. CONCLUSION: Sw-DESPOT offers a precise and fast method to monitor the CA dynamics in 3D volumes.

Evaluation of lung recovery after static administration of three different perfluorocarbons in pigs.

BACKGROUND: The respiratory properties of perfluorocarbons (PFC) have been widely studied for liquid ventilation in humans and animals. Several PFC were tested but their tolerance may depend on the species. Here, the effects of a single administration of liquid PFC into pig lungs were assessed and compared. Three different PFC having distinct evaporative and spreading coefficient properties were evaluated (Perfluorooctyl bromide [PFOB], perfluorodecalin [PFD] and perfluoro-N-octane [PFOC]). METHODS: Pigs were anesthetized and submitted to mechanical ventilation. They randomly received an intra-tracheal administration of 15 ml/kg of either PFOB, PFD or PFOC with 12 h of mechanical ventilation before awakening and weaning from ventilation. A Control group was submitted to mechanical ventilation with no PFC administration. All animals were followed during 4 days after the initial PFC administration to investigate gas exchanges and clinical recovery. They were ultimately euthanized for histological analyses and assessment of PFC residual concentrations within the lungs using dual nuclei fluorine and hydrogen Magnetic Resonance Imaging (MRI). Sixteen animals were included (4/group). RESULTS: In the PFD group, animals tended to be hypoxemic after awakening. In PFOB and PFOC groups, blood gases were not significantly different from the Control group after awakening. The poor tolerance of PFD was likely related to a large amount of residual PFC, as observed using MRI in all lung samples (≈10% of lung volume). This percentage was lower in the PFOB group (≈1%) but remained significantly greater than in the Control group. In the PFOC group, the percentage of residual PFC was not significantly different from that of the Control group (≈0.1%). Histologically, the most striking feature was an alveolar infiltration with foam macrophages, especially in the groups treated by PFD or PFOB. CONCLUSIONS: Of the three tested perfluorocarbons, PFOC offered the best tolerance in terms of lung function, gas exchanges and residuum in the lung. PFOC was rapidly cleared from the lungs and virtually disappeared after 4 days whereas PFOB persisted at significant levels and led to foam macrophage infiltration. PFOC could be relevant for short term total liquid ventilation with a rapid weaning.

Flow-compensated self-gating.

OBJECTIVE: Self-gating (SG) is a method to record cardiac movement during MR imaging. It uses information from an additional short, non-spatially encoded data acquisition. This usually lengthens TE and increases the sensitivity to flow artifacts. A new flow compensation scheme optimized for self-gating sequences is introduced that has very little or no time penalty over self-gating sequences without flow compensation. MATERIALS AND METHODS: Three variants of a self-gated 2D spoiled gradient echo or fast low angle shot (FLASH) sequence were implemented: without (noFC), with a conventional, serial (cFC), and with a new, time-efficient flow compensation (sFC). In experiments on volunteers and small animals, the sequence variants were compared with regard to the SG signal and the flow artifacts in the images. RESULTS: Both cFC and sFC reduce flow artifacts in cardiac images. The SG signal of the sFC is more sensitive to physiological motion, so that a cardiac trigger can be extracted more precisely as in cFC. In a typical setting for small animal imaging, sFC technique reduces the echo/repetition time over cFC by about 23%/14%. CONCLUSION: The time-efficient sFC technique provides flow-compensated images with cardiac triggering in both volunteers and small animals.

Polymer-dispersed liquid-crystal voltage sensor.

We present a novel electric-field and voltage sensor based on the electro-optical properties of polymer-dispersed liquid-crystals (PDLCs). In principle, the transmittance of PDLCs is a nonlinear function of the applied electrical field. To measure an AC field we superposed to it a known DC field. This allowed us to achieve linearization of the PDLC response and to measure transmittance changes independently of the light-intensity level variations. Validation experiments are presented.