Theoretical study of the Coriolis effect in LiNa, LiK, and LiRb molecules.

The non-adiabatic electronic matrix elements, LΠΣ(R), that arise from the spin-conserving electron-rotational interactions between all mΣ+ and mΠ states, where multiplicity m = 1, 3, converging to the lowest three dissociation limits of Li-containing alkali diatomics, LiM (M = Na, K, Rb), were calculated ab initio up to large internuclear distances, R. The required electronic wavefunctions were obtained within the framework of the multi-reference configuration interaction treatment of the two-valence-electron problem constructed using small-core scalar-relativistic effective core potentials and l-independent core-polarization potentials. A least squares analysis of the ab initio functions at large internuclear distances in conjunction with long-range perturbation theory (LRPT) revealed three different asymptotic behaviors of the LΠΣ(R → +∞)-functions: const. + ß[n]/Rn, characterized by n = -1, 3 and 6. The asymptotic coefficients ß[n], extracted from the point-wise ab initio data, were found to be in agreement with their LRPT counterparts, which were evaluated analytically using the relevant atomic parameters. The mass dependence of the LΠΣ matrix elements was investigated analytically and numerically. To confirm the reliability of the LΠΣ(R)-functions and interatomic potentials at small and intermediate distances, the empirical q-factors available for the D1Π-states of all LiM molecules studied were compared with their theoretical counterparts derived from the present ab initio data.

A first principles study of the spin-orbit coupling effect in LiM (M = Na, K, Rb, Cs) molecules.

The spin-orbit (SO) interactions in low-lying electronic states of the LiM (M = Na, K, Rb, Cs) molecular series are studied through ab initio calculations of potential energy curves and SO coupling matrix elements as functions of the interatomic distance, R. Two different approaches are employed: (a) the Fock-space relativistic coupled-cluster calculations (FS-RCC) which directly yield full relativistic energies, Urel(R); the SO coupling functions, ξso(R), are extracted a posteriori through projecting scalar-relativistic wave functions onto the subspaces spanned by their full-relativistic counterparts; (b) the evaluation of the scalar-relativistic electronic energies, Usr(R), and relevant ξso(R) functions using the configuration interaction method with core-valence correlation accounted for using core polarization potentials (CI-CPP). The SO-free potentials and SO coupling functions obtained within the framework of both approaches are in good agreement with each other and their prior theoretical and empirical counterparts.

Long-range behavior of the transition dipole moments of heteronuclear dimers XY (X, Y = Li, Na, K, Rb) based on ab initio calculations.

The ab initio electronic transition dipole moments (ETDMs) of heteronuclear dimers XY (X, Y = Li, Na, K, Rb) were calculated between the ground and excited states converging to the lowest three dissociation limits. The spin-allowed ETDMs were evaluated in a wide range of interatomic distances, R, by means of the quasi-relativistic electronic wave functions obtained by the multi-reference configuration interaction method. The inner-shell electrons (2 electrons for Li and Na atoms, and 10 and 28 for K and Rb, respectively) were described using the non-empirical shape-consistent effective core potentials. The l-independent core polarization potentials of each atom were used to take core-polarization and core-valence correlation effects into account. The long-range behavior of both singlet-singlet X1Σ+-(2,3)1Σ+;(1,2)1Π and triplet-triplet a3Σ+-(2,3)3Σ+;(1,2)3Π transition moments is perfectly fitted at large R-distance by the asymptotic formula of X. Chu and A. Dalgarno, Phys. Rev. A: At., Mol., Opt. Phys., 2002, 66, 024701: , where the coefficient ß is equal to 2 and -1 for the Σ-Σ and Σ-Π transitions, respectively. The n2S-n2P transition moments, dA, and dynamic polarizabilites, αB, of the alkali atoms in the n2S state extracted from the present molecular calculations coincide with their empirical and ab initio counterparts to within a few percent.

[The influence of risk factors for malignant tumors of maxillo-facial area on the expression of matrix metalloproteinases and tissue inhibitors of matrix metalloproteinases in patients of elderly and senile age period].

Morbidity and mortality from malignant neoplasms increases each year, and the average age of patients with first diagnosed decreases. The peak of incidence of malignant tumors of maxillofacial region falls on the elderly life. Not only age-related changes of the organism contribute in the development of this pathology, but also harmful habits. A comprehensive approach to solving the problem requires monitoring of this group of patients to identify risk factors for the development of pathologies in the oral and maxillofacial region.

[The diagnostic possibilities of saliva].

Saliva is a clinically informative biological fluid which contains multitude of bio-markers. This characteristic makes it possible to carry out numerous analyzes for developing mode to test patient in situ, express-tests included. The diagnostic by saliva is a new area of more simple application both markers and analyzers that can be useful in diagnostic of diseases of oral cavity, oncological diseases included. The using of saliva expands perspectives for making clinical diagnosis and establishment of dynamics and monitoring of disease.

[The application of certain methods of the statistical analysis for the determination of prescription of an injury to the spleen].

The objective of the present study was to elucidate specific morphological features of the injuries inflicted to the spleen that may be of value for determining their prescription by means of the sequential identification procedure with the use of the relevant diagnostic coefficients. The hierarchic cluster analysis has demonstrated that all the cases of interest can be categorized into 6 groups differing in the prescription of delayed injuries to the spleen that are possible to diagnose based on the morphological picture. It is concluded that the proposed sequential identification procedure with the use of the diagnostic coefficients makes it possible to distinguish between specific morphological features characterizing each group of splenic injuries.

[Correlation analysis of skin melanoma occurrence and major exogenic environmental factors in Samara region over 1995-2010].

The article covers study of major exogenic environmental factors influence on skin melanoma occurrence in Samara region, through application of various statistic analysis methods. The results prove increase in skin melanoma incidence in Samara region, that is characteristic for Russian population in general. Strong correlation is seen with increase of industrial waste volume in the region and with public nutrition structure changes.

[Forensic medical examination of a medical malpractice case as a form of situational expertise].

The authors propose to divide the process of forensic medical examination of a medical malpractice case into several stages and substantiate the possibility of their realization as a form of situational forensic medical expertise.

Immune Response of Mice Against Babesia canis Antigens is Enhanced When Antigen is Coupled to Gold Nanoparticles.

PURPOSE: The aim of this study was to isolate Babesia canis soluble antigens and to investigate the effect of their conjugates with gold nanoparticles on the immunogenicity in laboratory animals. METHODS: A procedure was developed for isolating and purifying B. canis antigens. The isolated culture antigen of B. canis 495 was coupled to gold nanoparticles, and the conjugate was used to immunize laboratory mice. RESULTS: Western blotting showed that the resultant antiserum specifically recognized the proteins of the B. canis strains isolated from naturally infected dogs. The antibody titer, the respiratory activity of peritoneal macrophages, the proliferative activity of splenocytes, and the production of cytokines were maximal when the animals were immunized with the antigen-nanoparticle conjugate emulsified in complete Freund's adjuvant. Without adjuvant, the babesial antigen was weakly immunogenic. CONCLUSION: Therefore, the use of gold nanoparticles as an antigen carrier induced a broad immune response involving both cellular and humoral responses. The antibodies raised by the proposed procedure are potentially effective at immunodetection of Babesia canis infections in dogs.

[Iatrogenic conditions as an object of forensic medical studies].

The author proposes an original definition of iatrogenic states and their forensic medical model based on the analysis of the causes underlying the development of these conditions. A rationale for the application of this model in the forensic medical practice is formulated.

A direct interaction of axonin-1 with NgCAM-related cell adhesion molecule (NrCAM) results in guidance, but not growth of commissural axons.

An interaction of growth cone axonin-1 with the floor-plate NgCAM-related cell adhesion molecule (NrCAM) was shown to play a crucial role in commissural axon guidance across the midline of the spinal cord. We now provide evidence that axonin-1 mediates a guidance signal without promoting axon elongation. In an in vitro assay, commissural axons grew preferentially on stripes coated with a mixture of NrCAM and NgCAM. This preference was abolished in the presence of anti-axonin-1 antibodies without a decrease in neurite length. Consistent with these findings, commissural axons in vivo only fail to extend along the longitudinal axis when both NrCAM and NgCAM interactions, but not when axonin-1 and NrCAM or axonin-1 and NgCAM interactions, are perturbed. Thus, we conclude that axonin-1 is involved in guidance of commissural axons without promoting their growth.

[Medical care of wounded persons with maxillofacial pathology in Main military hospital during the Great Patriotic War].

There was marked work of dental unit of the Main military hospital during the Great Patriotic War, including during evacuation in Gor'ky. Insertion of modern methods of immobilization of fragments of jaws and well organized succession to 1944 permitted to decrease the quantity of operations of traumatic osteomyelitis and increase the quantity of plastic operations. Owing to using of new materials (stainless steel, plastics) and to insertion of antibiotics, the period after 1946 is characterized by increased capabilities of effectuating of plastic operations and treatment of jaw breakups.

[Prehospital thrombolytic therapy with tenecteplase in patients with ST-elevation myocardial infarction].

We carried out prehospital thrombolytic therapy (TLT) with tenecteplase in 24 patients with ST-elevation myocardial infarction (MI) at the background of standard concomitant therapy with unfractionated heparin, aspirin, and clopidogrel and analyzed clinical course of MI, ECG dynamics, systolic left ventricular function according to echocardiography. In 20 patients coronary angiography was performed after TLT. Mean symptom - needle time was 113 min. In 14 patients (58%) TLT was carried out within 2 hours after appearance of symptoms. In 19 patients the course of disease was uncomplicated. Electrocardiographic criteria of effective reperfusion were registered in 14 patients (58%), angiographic criteria - in 13 patients (65% of subjected to angiography). Percutaneous intervention was performed in 18 patients (75%). Systolic left ventricular dysfunction was revealed in 5 patients (21%), aborted MI - in 4 patients. Significant correlation was found between aborted MI (r=0,38, p=0,034) and uncomplicated MI (r=0,40, p=0,027) and performance of TLT during first 2 hours. There were no hemorrhagic complications. Results of the study evidence for high efficacy of prehospital thrombolysis with tenecteplase and real possibility of its use under conditions of existing system of organization of urgent cardiological aid.

[Surgical treatment of gastroesophageal reflux disease combined with duodenal ulcer].

Operations for ulcer disease of the duodenum were performed on 696 patients. In 112 (16.1%) patients there were symptoms of insufficient obturating function of the cardia, which was considered to be an indication to antireflux simultaneous operation on the cardia. The indications were worked out for the decision on operative methods in patients with ulcer disease of the duodenum in combination with gastroesophageal reflux disease. Methods of resection were used in 88 (78.5%) patients, videolaparoscopic selective proximal vagotomy with an original means of fundoplication--in 24 (21.5%) patients. Good and excellent long-term results after operation were noted in 95.5% of the patients.

[Functional results of the surgery of esophageal opening hernia].

The article presents an original method of laparoscopic anti-reflux surgery, immediate and long-term results of the treatment of 132 patients with esophageal opening hernia. It also describes the analysis of the life quality of patients with the gastroesophageal reflux disease before and after the surgery.

[Method of treatment of reflux-esophagitis after gastrectomy].

A new method of treatment of reflux-esophagitis developed for patients after gastrectomy has a distinctive moment: the formation of a restraining mechanism in the field of esophago-gastric anastomosis, without opening the intestinal tube lumen. The carried on trials of clinical application of the method have shown its high effectiveness for prophylactics ofjejuno-esophageal reflux both in the early and distant terms after operation. The method of operating was given a patent of the Russian Federation.

[Experimental grounds for the formation of an increased pressure zone in the area of "impaired" lower esophageal sphincter].

A method of the formation of an artificial lower esophageal sphincter (LES) with the help of superelastic threads made of a nickelide-titan alloy was developed in the experiment. An experimental model of an artificial LES was tested on cadaver organic combinations of the esophagus and stomach in ten watchdogs and ten rabbits. Tests of the esophageal passage and antireflux features of the artificial lower esophageal sphincter (LES) and assessment of morphological changes in esophageal wall layers with the emphasis on changes of the esophageal wall structure and gastroesophageal passage near the thread were made. This experiment proved that the passage in the artificial lower esophageal sphincter (LES) area is not disturbed and a zone of increased pressure is created.

Structure of the mouse gene for the serine protease inhibitor neuroserpin (PI12).

Neuroserpin (PI12), initially identified as an axonally secreted protein in cultured chicken dorsal root ganglion neurons, belongs to the serpin family of the serine protease inhibitors and is mainly expressed by neurons of both the developing and the adult nervous system. Here we report on the cloning and structural characterization of the neuroserpin gene of the mouse. The murine neuroserpin gene spans over more than 55kb and consists of nine exons. The positions and phases of the exonintron borders are completely conserved between neuroserpin and its nearest homologues, protease nexin-1 and plasminogen activator inhibitor-1. A single transcription initiation site, which is colocalized with a potential initiation (Inr) sequence, has been determined by primer extension and RNase protection. Sequence analysis revealed a TATA-less promoter with a CAAT box and several sites for the general transcription factor Sp1 and the neuron-specific transcription factor AP-2.

Crystal structure of neuroserpin: a neuronal serpin involved in a conformational disease.

The protease inhibitor neuroserpin regulates the development of the nervous system and its plasticity in the adult. Neuroserpins carrying the Ser53Pro or Ser56Arg mutation form polymers in neuronal cells. We describe here the structure of wild-type neuroserpin in a cleaved form. The structure provides a basis to understand the role of the mutations in the polymerization process. We propose that these mutations could delay the insertion of the reactive center loop into the central beta-sheet A, an essential step in the inhibition and possibly in the polymerization of neuroserpin.

[Clinical picture and diagnosis of the Mills syndrome]. / O klinike i diagnostike sindroma Millsa.

Three clinical observations of patients with Mills' syndrome are presented. The clinical peculiarity of the syndrome and the specificity of the pathological process localization in the brain revealed on pneumoencephalographic examination are stressed. The atrophic process in the fronto-parietal parasaggital divisions of the cerebral cortex is regarded as a morphological substrate of the syndrome. A conclusion on the vagueness of the process character and prematurity of the pathogenetic correlations is drawn. Nosological independence of the pathology is not improbable.