Less Invasive Vestibule Access Tunneling with Platelet-rich Fibrin Membrane for the Treatment of Gingival Recession.

AIM: The aim of this study was to introduce the less invasive vestibule access tunneling in combination with platelet-rich fibrin (PRF) membrane for gingival recession treatment. MATERIALS AND METHODS: Seven subjects with Miller's class I or II buccal gingival recession were selected for this study. All subjects were treated with mucogingival surgery using the less invasive vestibule access tunneling in combination with PRF membrane. Clinical examination performed on each subject and the height of gingival recession was recorded preoperatively (baseline), and at 1 and 3 months postoperatively. The data were statistically analyzed using analysis of variance (ANOVA) with post hoc Tukey's LSD test to determine the significant difference between groups. Statistical significance level was set at 0.05. RESULTS: The results showed that there were significant differences in recession height between baseline and both 1 month and 3 months postoperatively (p < 0.05). Nevertheless, there was no significant difference between 1 and 3 months postoperatively (p > 0.05). All patients reported satisfactory esthetic results both at 1 and 3 months postoperatively. CONCLUSION: Mucogingival surgery using the less invasive vestibule access tunneling in combination with PRF membrane for gingival recession treatment provided optimal root coverage. CLINICAL SIGNIFICANCE: This article introduces a new tunnel technique in combination with PRF membrane for gingival recession treatment. Previous studies tried to make access using the tunnel technique performed through gingival sulcus. In this study, the access is performed through the vestibule region. Access from the vestibule can minimize the damage of gingival margin integrity, especially in patients with thin gingival biotype.

The contradictory effects of coffee intake on periodontal health: a systematic review of experimental and observational studies.

Background: Drinking coffee is known to have both positive and negative aftermath on periodontal health. The current study is aiming to systematically review the impact of coffee consumption on periodontal health status. Methods: An article search was carried out in two electronic databases (PUBMED and Web of Sciences). All type of experimental and observational studies were included. The assessment of the included articles were conducted using Joanna Briggs Institute (JBI) critical appraisal tool. Data were analyzed qualitatively. Result: A total of 10 articles were included in this study. Most (5) of the studies discovered a negative correlation between coffee intake and periodontal health, while 4 other studies found the protective effect of daily coffee consumption against alveolar bone loss. Last, only one study found that coffee intake did not relate with periodontitis. Conclusion: The effect of coffee consumption on periodontal health was fragmented since coffee has complex components that may give either beneficial effects or negative impact on periodontal health.

Transforming Growth Factor-ß1 and Runt-related Transcription Factor 2 as Markers of Osteogenesis in Stem Cells from Human Exfoliated Deciduous Teeth Enriched Bone Grafting.

BACKGROUND: Stem cells from human exfoliated deciduous teeth (SHED) are one source of adult stem cells which can proliferate and differentiate into many types of tissues than any other stem cells. SHED represent potential stem cells for therapeutic therapy and tissue engineering. AIMS: The aim of this study was to compare the expression of transforming growth factor-ß1 (TGF-ß1) and runt-related transcription factor 2 (RUNX2) in hydroxyapatite (HA) scaffold with SHED. SUBJECTS AND METHODS: Eight experimental animals were divided into two groups. The first group was transplanted with HA and the second with HA and SHED. The expression of TGF-ß1 and RUNX2 was seen 21 days later by means of immunohistochemical analysis. STATISTICAL ANALYSIS USED: Data were analyzed using an independent t-test with a significance level of 5%. RESULTS: The analysis results of an independent t-test showed a significant difference between the two groups. The second group given HA with SHED showed a significantly higher expression of TGF-ß1 and RUNX2 than that of the first group. CONCLUSIONS: Expression of TGF-ß1 and RUNX2 occurs after the application of HA with SHED, while TGF-ß1 and RUNX2 expression in the HA with SHED group was significantly higher than in the group without SHED.