Chondrocyte Isolation from Loose Bodies-An Option for Reducing Donor Site Morbidity for Autologous Chondrocyte Implantation.

Loose bodies (LBs) from patients with osteochondritis dissecans (OCD) are usually removed and discarded during surgical treatment of the defect. In this study, we address the question of whether these LBs contain sufficient viable and functional chondrocytes that could serve as a source for autologous chondrocyte implantation (ACI) and how the required prolonged in vitro expansion affects their phenotype. Chondrocytes were isolated from LBs of 18 patients and compared with control chondrocyte from non-weight-bearing joint regions (n = 7) and bone marrow mesenchymal stromal cells (BMSCs, n = 6) obtained during primary arthroplasty. No significant differences in the initial cell yield per isolation and the expression of the chondrocyte progenitor cell markers CD44 + /CD146+ were found between chondrocyte populations from LBs (LB-CH) and control patients (Ctrl-CH). During long-term expansion, LB-CH exhibited comparable viability and proliferation rates to control cells and no ultimate cell cycle arrest was observed within 12 passages respectively 15.3 ± 1.1 mean cumulative populations doublings (CPD). The chondrogenic differentiation potential was comparable between LB-CH and Ctrl-CH, but both groups showed a significantly higher ability to form a hyaline cartilage matrix in vitro than BMSC. Our data suggest that LBs are a promising cell source for obtaining qualitatively and quantitatively suitable chondrocytes for therapeutic applications, thereby circumventing donor site morbidity as a consequence of the biopsies required for the current ACI procedure.

Treatment of osteochondral defects with a combination of bone grafting and AMIC technique.

INTRODUCTION: Osteochondral defects of the knee may cause functional impairment of young and sportively active patients. Different surgical treatment options have been proposed using either one or two step procedures. The aim of the current study was to evaluate mid-term outcomes of combined bone grafting with autologous matrix-associated chondrogenesis (AMIC) for the treatment of large osteochondral defects. MATERIALS AND METHODS: 15 Patients with osteochondrosis dissecans of the medial femoral condyle grade III or IV according to ICRS classification were treated with a single step surgical procedure combining bone grafting and the AMIC procedure. Mean defect size was 4.98 cm2 (± 3.02) and patients were examined at 6, 12 weeks, 6 and 12 month and at mean final follow-up of 49 months (36-61). Patients were evaluated using VAS, IKDC, KOOS, Lysholm, Tegner activity scores and psychological and physical health assessed using the SF 12. MRI evaluation was performed at final follow-up using the MOCART score. RESULTS: Pain had significantly decreased at final follow-up (7.2 ± 1.4 vs. 2.4 ± 2.6) compared to preoperative baseline. All functional scores had improved significantly throughout the follow-up period (IKDC from 36.6 ± 20.6 vs. 72.2 ± 18.7; KOOS 50.0 ± 18.9 vs. 81.7 ± 13.9; LYSHOLM 39.3 ± 19.5 vs. 79.8 ± 15.1). SF12 evaluation showed a significant increase in physical component summary (PCS) (31.2 ± 11.1 preoperative vs. 46.3 ± 9.9 at final follow-up), while mental component summary (MCS) remained stable (51.8 ± 8.9 vs. 57.3 ± 3.3). MOCART score revealed a mean overall score of 77 ± 15 at final follow-up. Integration to the adjacent cartilage was complete in 79%, incomplete in 21%. Defect filling was complete in 64%, incomplete in 36%. CONCLUSION: Significant improvement of knee function and restoration of homogenous cartilage morphology could be achieved with simultaneous AMIC procedure and bone grafting in 2/3 of all patients with large osteochondral lesions at 4 years postoperatively.

[Reconstruction of large acetabular defects using trabecular metal augments]. / Rekonstruktion grösserer Pfannendefekte mit metallischen Augmentaten--Trabecular Metal Technology.

OBJECTIVE: Revision of cup and reconstruction of original center of rotation. High primary and secondary stability. Prevention of additional bone loss. INDICATIONS: Osseous defects at the anterior-cranial, cranial and posterior-cranial rim of acetabulum. Larger cavitary, medial or oval defects (Paprosky IIb-IIIb). Segmental defects (anterior column up to half of host bone, posterior column up to one third of host bone). CONTRAINDICATIONS: Infection of total hip arthroplasty. Pelvic discontinuity (Paprosky IV). SURGICAL TECHNIQUE: Exposure of acetabulum and detection of defects. Complete removal of soft tissue from acetabulum, reaming of sclerotic bone, if necessary. Adaptation of trial augments to close an oval defect to a round defect and to reach an uncontained defect, respectively. Adaptation of trial cup. In case of sufficient stability, fixation of final augment with two or three screws in cranial bone stock. The screws should be directed to iliosacral joint. Augmentation with allogenic bone chips is possible in the region of wedge and acetabulum as well. Sealing of rough augment surface with bone cement. Implantation of cup, fixation with screws. Application of insert. POSTOPERATIVE MANAGEMENT: Depending on bone defects, full weight bearing is possible. In cases of severe bone defects, reduction of weight bearing to 20 kg for 6 weeks is recommended. Postoperative physiotherapy is possible in most cases. RESULTS: Between 2005 and 2007, 38 patients with acetabular defects type IIIa und IIIb according to Paprosky underwent reconstruction using the TMT system (Trabecular Metal Technology). After 25 months, a significant functional improvement was seen in all patients. The Merle d'Aubigné Score increased from 6 points preoperatively to 13 points postoperatively, the Harris Hip Score from 29 to 78 points. Two revisions were necessary because of loosening or migration of the cup.