Parallel evolution in an island archipelago revealed by genomic sequencing of Hipposideros leaf-nosed bats.

Body size is a key morphological attribute, often used to delimit species boundaries among closely related taxa. But body size can evolve in parallel, reaching similar final states despite independent evolutionary and geographic origins, leading to faulty assumptions of evolutionary history. Here, we document parallel evolution in body size in the widely distributed leaf-nosed bat genus Hipposideros, which has misled both taxonomic and evolutionary inference. We sequenced reduced representation genomic loci and measured external morphological characters from three closely related species from the Solomon Islands archipelago, delimited by body size. Species tree reconstruction confirms the paraphyly of two morphologically designated species. The nonsister relationship between large-bodied H. dinops lineages found on different islands indicates that large-bodied ecomorphs have evolved independently at least twice in the history of this radiation. A lack of evidence for gene flow between sympatric, closely related taxa suggests the rapid evolution of strong reproductive isolating barriers between morphologically distinct populations. Our results position Solomon Islands Hipposideros as a novel vertebrate system for studying the repeatability of parallel evolution under natural conditions. We conclude by offering testable hypotheses for how geography and ecology could be mediating the repeated evolution of large-bodied Hipposideros lineages in the Solomon Islands.

Intraguild relationships between sympatric predators exposed to lethal control: predator manipulation experiments.

INTRODUCTION: Terrestrial top-predators are expected to regulate and stabilise food webs through their consumptive and non-consumptive effects on sympatric mesopredators and prey. The lethal control of top-predators has therefore been predicted to inhibit top-predator function, generate the release of mesopredators and indirectly harm native fauna through trophic cascade effects. Understanding the outcomes of lethal control on interactions within terrestrial predator guilds is important for zoologists, conservation biologists and wildlife managers. However, few studies have the capacity to test these predictions experimentally, and no such studies have previously been conducted on the eclectic suite of native and exotic, mammalian and reptilian taxa we simultaneously assess. We conducted a series of landscape-scale, multi-year, manipulative experiments at nine sites spanning five ecosystem types across the Australian continental rangelands to investigate the responses of mesopredators (red foxes, feral cats and goannas) to contemporary poison-baiting programs intended to control top-predators (dingoes) for livestock protection. RESULT: Short-term behavioural releases of mesopredators were not apparent, and in almost all cases, the three mesopredators we assessed were in similar or greater abundance in unbaited areas relative to baited areas, with mesopredator abundance trends typically either uncorrelated or positively correlated with top-predator abundance trends over time. The exotic mammals and native reptile we assessed responded similarly (poorly) to top-predator population manipulation. This is because poison baits were taken by multiple target and non-target predators and top-predator populations quickly recovered to pre-control levels, thus reducing the overall impact of baiting on top-predators and averting a trophic cascade. CONCLUSIONS: These results are in accord with other predator manipulation experiments conducted worldwide, and suggest that Australian populations of native prey fauna at lower trophic levels are unlikely to be negatively affected by contemporary dingo control practices through the release of mesopredators. We conclude that contemporary lethal control practices used on some top-predator populations do not produce the conditions required to generate positive responses from mesopredators. Functional relationships between sympatric terrestrial predators may not be altered by exposure to spatially and temporally sporadic application of non-selective lethal control.

Plastic ingestion by fishing cats suggests trophic transfer in urban wetlands.

Recent studies have suggested that plastic contamination in some terrestrial and freshwater environments is estimated to be greater than that detected in marine environments. Urban wetlands are prone to plastic pollution but levels of contamination in their wildlife are poorly quantified. We collected 276 fishing cat (Prionailurus viverrinus) scat samples in Colombo, Sri Lanka for a dietary study of urban fishing cats. We used traditional dietary analysis methodology to investigate the contents of the scats by washing, isolating, and identifying prey remains; while sorting prey remains of individual scats, we unexpectedly detected macroscopic (>1 mm) plastic debris in six (2.17%) of the samples. Across all scat samples, we detected low occurrences of microplastics (0.72%), mesoplastics (1.09%) and macroplastics (1.45%). All three plastic types were found in scats containing rodent remains, while meso-, and macroplastics were found in scats with avian remains, and micro- and macroplastics in scats containing freshwater fish remains. Given that felids are obligate generalist carnivores that eat live or recently dead prey and do not consume garbage, our findings suggest that trophic transfer of plastics occurred whereby fishing cats consumed prey contaminated with plastic. Although macroscopic plastic detection was low, our findings suggest that accumulation of plastics is occurring in wetland food webs, and plastic pollution in freshwater terrestrial systems could pose a risk to predators that do not directly consume plastics but inhabit contaminated environments.

Social interactions of juvenile rabbits (Oryctolagus cuniculus) and their potential role in lagovirus transmission.

Rabbit Haemorrhagic Disease Virus (RHDV), which is a calicivirus, is used as a biocontrol agent to suppress European wild rabbit populations in Australia. The transmission of RHDV can be influenced by social interactions of rabbits; however, there is a paucity of this knowledge about juvenile rabbits and the roles they may play in the transmission of RHDV. We aimed to quantify the social interactions of juvenile (< 900 g) and adult (> 1200 g) rabbits in a locally abundant population in the Central Tablelands of New South Wales, Australia. Twenty-six juvenile and 16 adult rabbits were fitted with VHF proximity loggers to monitor intra- and inter-group pairings. Use of multiple warrens by these rabbits was investigated using VHF base stations at nine warrens and on foot with a hand-held Yagi antenna. Juvenile rabbits were strongly interconnected with both juveniles and adults within and outside their warren of capture, and almost all juveniles were well-connected to other individuals within their own social group. Inter-group pairings were infrequent and fleeting between adults. Both juvenile and adult rabbits used multiple warrens. However, visits to warrens outside their warren of capture, particularly those within 50 m, were more common and longer in duration in juveniles than in adults. The high connectivity of juveniles within and between warrens in close proximity increases potential pathogen exchange between warrens. Therefore, juvenile rabbits could be of greater importance in lagovirus transmission than adult rabbits. The strength of juvenile rabbit inter- and intra-group pairings, and their tendency to use multiple warrens, highlight their potential to act as 'superspreaders' of both infection and immunity for lagoviruses and other pathogens with similar lifecycles. Confirmation of this potential is required through examination of disease progress and rabbit age-related immune responses during outbreaks.

Does the house mouse self-regulate its density in maturing sorghum and wheat crops?

1. One of the central questions in population ecology and management is: what regulates population growth? House mouse Mus domesticus L. populations erupt occasionally in grain-growing regions in Australia. This study aimed to determine whether mouse populations are self-regulated in maturing sorghum and wheat crops. This was assessed by examining food supply to mice (i.e. yield) and the relationship between initial mouse density (D(I)) and density at harvest (D(H)). Eight levels of D(I) ranging from 89 to 5555 mice ha(-1) were introduced to sorghum at the hard dough stage and to wheat crops at the milky stage in mouse-proofed pens. D(H) was measured by trapping out mice 49 days after the introduction. 2. There were at least 3.11 tonnes ha(-1) of wheat and 1.85 tonnes ha(-1) of sorghum grain available for mice at harvest. The estimated relationship between D(I) and D(H) was asymptotic exponential, with D(H) initially increasing almost linearly with D(I). When D(I) was above c. 500 mice ha(-1), D(H) increased asymptotically with D(I) and then saturated at c. 3100 mice ha(-1). The asymptotic increases in and saturation of D(H) was due partly to more young mice being born and recruited in pens treated with lower levels of D(I). 3. Our findings indicated that mouse densities in maturing cereal crops were driven by a numerical response of mice to the abundant supply of grain, modified by some unknown self-regulation mechanism that reduced this numerical response of mice at higher mouse densities. The mechanism was possibly spacing behaviours. Although the nature of this self-regulation mechanism is not known our model is, nevertheless, useful for predicting increases and eruptions in mouse population density in sorghum and wheat crops. Understanding the nature of this mechanism may provide insights into population processes that can be exploited in controlling mice in cereal crops.

Environmental effects are stronger than human effects on mammalian predator-prey relationships in arid Australian ecosystems.

Climate (drought, rainfall), geology (habitat availability), land use change (provision of artificial waterpoints, introduction of livestock), invasive species (competition, predation), and direct human intervention (lethal control of top-predators) have each been identified as processes driving the sustainability of threatened fauna populations. We used a systematic combination of empirical observational studies and experimental manipulations to comprehensively evaluate the effects of these process on a model endangered rodent, dusky hopping-mice (Notomys fuscus). We established a large manipulative experiment in arid Australia, and collected information from relative abundance indices, camera traps, GPS-collared dingoes (Canis familiaris) and dingo scats, along with a range of related environmental data (e.g. rainfall, habitat type, distance to artificial water etc.). We show that hopping-mice populations were most strongly influenced by geological and climatic effects of resource availability and rainfall, and not land use, invasive species, or human effects of livestock grazing, waterpoint provision, or the lethal control of dingoes. Hopping-mice distribution declined along a geological gradient of more to less available hopping-mice habitat (sand dunes), and their abundance was driven by rainfall. Hopping-mice populations fluctuated independent of livestock presence, artificial waterpoint availability or repeated lethal dingo control. Hopping-mice populations appear to be limited first by habitat availability, then by food availability, then by predation. Contemporary top-predator control practices (for protection of livestock) have little influence on hopping-mice behaviour or population dynamics. Given our inability to constrain the effects of predation across broad scales, management actions focusing on increasing available food and habitat (e.g. alteration of fire and herbivory) may have a greater chance of improving the conservation status of hopping-mice and other small mammals in arid areas. Our study also reaffirms the importance of using systematic and experimental approaches to detect true drivers of population distribution and dynamics where multiple potential drivers operate simultaneously.

The Distribution and Density of Water Mice (Xeromys myoides) in the Maroochy River of Southeast Queensland, Australia.

The water mouse is a small and vulnerable rodent present in coastal areas of south-west Papua New Guinea, and eastern Queensland and the Northern Territory of Australia. Current knowledge regarding the distribution of the water mouse is incomplete and the loss of one local population has been documented in southeast Queensland, a region where pressures from urban and industrial development are increasing. Water mouse populations have not been studied intensively enough to enable the primary factors responsible for the local decline to be identified. We surveyed the distribution and density of the water mouse along the Maroochy River of southeast Queensland, near the southern extent of the species' range, to gather baseline data that may prove valuable for detecting any future decline in this population's size or health. All areas of suitable habitat were surveyed on foot or by kayak or boat over a three-year period. We found 180 water mouse nests, of which ~94% were active. Permanent camera monitoring of one nest and limited supplementary live trapping suggested that up to three individual mice occupied active nests. Water mouse density was estimated to be 0.44 per hectare of suitable habitat along the Maroochy River. Should future monitoring reveal an adverse change in the water mouse population on the Maroochy River, a concerted effort should be made to identify contributing factors and address proximate reasons for the decline.

Dingoes at the Doorstep: Home Range Sizes and Activity Patterns of Dingoes and Other Wild Dogs around Urban Areas of North-Eastern Australia.

Top-predators around the world are becoming increasingly intertwined with humans, sometimes causing conflict and increasing safety risks in urban areas. In Australia, dingoes and dingo×domesticdoghybridsarecommoninmanyurbanareas,andposeavarietyofhumanhealth and safety risks. However, data on urban dingo ecology is scant. We GPS-collared 37 dingoes in north-easternAustraliaandcontinuouslymonitoredthemeach30minfor11-394days. Mostdingoes were nocturnal, with an overall mean home range size of 17.47 km2. Overall mean daily distance travelled was 6.86 km/day. At all times dingoes were within 1000 m of houses and buildings. Home ranges appeared to be constrained to patches of suitable vegetation fragments within and around human habitation. These data can be used to reallocate dingo management effort towards mitigating actual conflicts between humans and dingoes in urban areas.

Insects for breakfast and whales for dinner: the diet and body condition of dingoes on Fraser Island (K'gari).

Top-predators play stabilising roles in island food webs, including Fraser Island, Australia. Subsidising generalist predators with human-sourced food could disrupt this balance, but has been proposed to improve the overall health of the island's dingo (Canis lupus dingo) population, which is allegedly 'starving' or in 'poor condition'. We assess this hypothesis by describing the diet and health of dingoes on Fraser Island from datasets collected between 2001 and 2015. Medium-sized mammals (such as bandicoots) and fish were the most common food items detected in dingo scat records. Stomach contents records revealed additional information on diet, such as the occurrence of human-sourced foods. Trail camera records highlighted dingo utilisation of stranded marine fauna, particularly turtles and whales. Mean adult body weights were higher than the national average, body condition scores and abundant-excessive fat reserves indicated a generally ideal-heavy physical condition, and parasite loads were low and comparable to other dingo populations. These data do not support hypotheses that Fraser Island dingoes have restricted diets or are in poor physical condition. Rather, they indicate that dingoes on Fraser Island are capable of exploiting a diverse array of food sources which contributes to the vast majority of dingoes being of good-excellent physical condition.

Diet of dingoes and other wild dogs in peri-urban areas of north-eastern Australia.

Knowledge of the resource requirements of urban predators can improve our understanding of their ecology and assist town planners and wildlife management agencies in developing management approaches that alleviate human-wildlife conflicts. Here we examine food and dietary items identified in scats of dingoes in peri-urban areas of north-eastern Australia to better understand their resource requirements and the potential for dingoes to threaten locally fragmented populations of native fauna. Our primary aim was to determine what peri-urban dingoes eat, and whether or not this differs between regions. We identified over 40 different food items in dingo scats, almost all of which were mammals. Individual species commonly observed in dingo scats included agile wallabies, northern brown bandicoots and swamp wallabies. Birds were relatively common in some areas but not others, as were invertebrates. Dingoes were identified as a significant potential threat to fragmented populations of koalas. Dietary overlap was typically very high or near-identical between regions, indicating that peri-urban dingoes ate the same types or sizes of prey in different areas. Future studies should seek to quantify actual and perceived impacts of, and human attitudes towards, peri-urban dingoes, and to develop management strategies with a greater chance of reducing human-wildlife conflicts.

The (non)effects of lethal population control on the diet of Australian dingoes.

Top-predators contribute to ecosystem resilience, yet individuals or populations are often subject to lethal control to protect livestock, managed game or humans from predation. Such management actions sometimes attract concern that lethal control might affect top-predator function in ways ultimately detrimental to biodiversity conservation. The primary function of a predator is predation, which is often investigated by assessing their diet. We therefore use data on prey remains found in 4,298 Australian dingo scats systematically collected from three arid sites over a four year period to experimentally assess the effects of repeated broad-scale poison-baiting programs on dingo diet. Indices of dingo dietary diversity and similarity were either identical or near-identical in baited and adjacent unbaited treatment areas in each case, demonstrating no control-induced change to dingo diets. Associated studies on dingoes' movement behaviour and interactions with sympatric mesopredators were similarly unaffected by poison-baiting. These results indicate that mid-sized top-predators with flexible and generalist diets (such as dingoes) may be resilient to ongoing and moderate levels of population control without substantial alteration of their diets and other related aspects of their ecological function.

The short-term effects of a routine poisoning campaign on the movements and detectability of a social top-predator.

Top-predators can be important components of resilient ecosystems, but they are still controlled in many places to mitigate a variety of economic, environmental and/or social impacts. Lethal control is often achieved through the broad-scale application of poisoned baits. Understanding the direct and indirect effects of such lethal control on subsequent movements and behaviour of survivors is an important pre-requisite for interpreting the efficacy and ecological outcomes of top-predator control. In this study, we use GPS tracking collars to investigate the fine-scale and short-term movements of dingoes (Canis lupus dingo and other wild dogs) in response to a routine poison-baiting program as an example of how a common, social top-predator can respond (behaviourally) to moderate levels of population reduction. We found no consistent control-induced differences in home range size or location, daily distance travelled, speed of travel, temporal activity patterns or road/trail usage for the seven surviving dingoes we monitored immediately before and after a typical lethal control event. These data suggest that the spatial behaviour of surviving dingoes was not altered in ways likely to affect their detectability, and if control-induced changes in dingoes' ecological function did occur, these may not be related to altered spatial behaviour or movement patterns.

Resource pulses in desert river habitats: productivity-biodiversity hotspots, or mirages?

Resource pulses in the world's hot deserts are driven largely by rainfall and are highly variable in both time and space. However, run-on areas and drainage lines in arid regions receive more water more often than adjacent habitats, and frequently sustain relatively high levels of primary productivity. These landscape features therefore may support higher biotic diversity than other habitats, and potentially act as refuges for desert vertebrates and other biota during droughts. We used the ephemeral Field River in the Simpson Desert, central Australia, as a case study to quantify how resources and habitat characteristics vary spatially and temporally along the riparian corridor. Levels of moisture and nutrients were greater in the clay-dominated soils of the riverine corridor than in the surrounding sand dunes, as were cover values of trees, annual grasses, other annual plants and litter; these resources and habitat features were also greater near the main catchment area than in the distal reaches where the river channel runs out into extensive dune fields. These observations confirm that the riverine corridor is more productive than the surrounding desert, and support the idea that it may act as a refuge or as a channel for the ingress of peri-desert species. However, the work also demonstrates that species diversity of invertebrates and plants is not higher within the river corridor; rather, it is driven by rainfall and the accompanying increase in annual plants following a rain event. Further research is required to identify the biota that depend upon these resource pulses.

Assessing predation risk to threatened fauna from their prevalence in predator scats: dingoes and rodents in arid Australia.

The prevalence of threatened species in predator scats has often been used to gauge the risks that predators pose to threatened species, with the infrequent occurrence of a given species often considered indicative of negligible predation risks. In this study, data from 4087 dingo (Canis lupus dingo and hybrids) scats were assessed alongside additional information on predator and prey distribution, dingo control effort and predation rates to evaluate whether or not the observed frequency of threatened species in dingo scats warrants more detailed investigation of dingo predation risks to them. Three small rodents (dusky hopping-mice Notomys fuscus; fawn hopping-mice Notomys cervinus; plains mice Pseudomys australis) were the only threatened species detected in <8% of dingo scats from any given site, suggesting that dingoes might not threaten them. However, consideration of dingo control effort revealed that plains mice distribution has largely retracted to the area where dingoes have been most heavily subjected to lethal control. Assessing the hypothetical predation rates of dingoes on dusky hopping-mice revealed that dingo predation alone has the potential to depopulate local hopping-mice populations within a few months. It was concluded that the occurrence of a given prey species in predator scats may be indicative of what the predator ate under the prevailing conditions, but in isolation, such data can have a poor ability to inform predation risk assessments. Some populations of threatened fauna assumed to derive a benefit from the presence of dingoes may instead be susceptible to dingo-induced declines under certain conditions.

Managing crop damage caused by house mice (Mus domesticus) in Australia.

A large-scale outbreak of the house mouse populations occurs in grain growing in Australia on average once every four years. High densities of mice cause major yield losses to cereal crops, and low to moderate densities of mice also cause some losses. Several predictive models based on rainfall patterns have been developed to forecast mouse density. These models carry some uncertainty and the economic value of basing management actions on these models is not clear. Baiting is the most commonly used method and zinc phosphide and other rodenticide bait are effective in reducing up to 90% of mouse populations. Ecologically-based best farming practice for controlling mice has recently been developed on the basis of long-term field studies of mouse populations. No effective biological control method has been developed for mice. However, grain growers still cannot make economically rational decisions to implement control because they do not know the pest threshold density (D(T)) above which the economic benefits of control exceed the economic costs of control. Applied predator-prey theory suggests that understanding the relationship between mouse density and damage is the basis for determining D(T). Understanding this relationship is the first research priority for managing mouse damage. The other research priority is to develop a reliable method to estimate unbiased mouse density.

Comparison of population estimators and indices for monitoring house mice in sorghum crops.

House mice (Mus musculus domesticus Schwarz & Schwarz, 1943) are monitored in Australia and China to track changes in mouse population densities and forecast their potential damage to cereal crops. The present study compared population indices based on the number of different mice caught and overall trap success from live-trapping with an oil card index (OC) and a tracking index (T) for monitoring mice in sorghum crops immediately before crop maturation. T was measured as the percentage of track board covered with mouse footprints night(-1), and OC as the percentage of card removed by mice night(-1). The reliability of these abundance indices was quantified by Pearson correlation coefficient with the trappable population size (Ñ), which was estimated by capture-recapture over eight consecutive nights on 175 × 5 trapping grids, in sorghum crops on two properties on the Darling Downs, Queensland. Because of differences among individual mice in capture probability, Model M(h) of program MARK was used to account for such heterogeneity and to estimate the size of each mouse population. The number of individual animals caught was more strongly correlated with Ñ than trap success and, therefore, might be a more reliable index; the data suggest that three trapping occasions provide optimal precision for this index. T correlated significantly with Ñ only at sites where the canopy of sorghum plants was closed, and its use should, therefore, be restricted to this habitat. OC did not correlate with Ñ because none or very little of the cards was eaten at low to moderate mouse densities. T and the number of animals caught over three trapping nights are recommended for monitoring mice in sorghum crops immediately prior to crop maturation.

A rare autosomal recessive condition, congenital erythropoietic porphyria, found in the canefield rat Rattus sordidus Gould 1858.

Congenital erythropoietic porphyria (CEP) is a rare autosomal recessive condition that has been reported in humans and in some animals, in which uroporphyrin 1 is deposited in the bones, teeth and urine, resulting in pink coloration and fluorescence of the tissues and urine under long-wave ultraviolet (UV) light. We observed red teeth in nine of 450 canefield rats (Rattus sordidus) captured in a small, isolated patch of sugarcane in Tully, north Queensland, Australia. The skeletons of these animals were excised and were found to be bright red under normal day light. Under UV light, the skeleton had a bright red fluorescence. It is plausible that the canefield rat population in this isolated patch of sugarcane is small and inbreeding might have occurred, resulting in incidences of the autosomal recessive genes that cause CEP. The canefield rat can be used as an animal model for research into porphyria.

Grain, pellet and wax block bait take by the house mouse (Mus musculus) and non-target species: implications for mouse eradications on coral cay islands.

Introduced rodents have been eradicated from large numbers of offshore islands using toxic baits; however, toxic baits have been linked with negative impacts on non-target species. The present study assessed the bait take of target (house mouse, Mus musculus) and non-target (buff banded rail, Rallus philippensis) animals on Northwest and Heron Islands in the Great Barrier Reef. Three non-toxic bait formulations (wax block, pellet and grain) were tested and each was applied at 1 kg ha(-1) in six treatment grids. The tracks of animals visiting the baits were identified using 30 tracking stations per treatment grid. A tracking station consisted of a track-board placed in the centre of a sand-pad. Mean bait take differed significantly between the formulations: birds took more grain bait than wax block bait; mice took more wax block than grain bait. Both mice and birds were equally selective of pellet bait. Thus, the findings indicate that wax blocks are the most suitable formulation for future baiting programs to eradicate mice on these and other islands.