RESUMEN
Four new nardosinane-type sesquiterpenoids, flavalins A-D (1-4), have been isolated from the Formosan soft coral Lemnalia flava. The structures of the new metabolites were determined by extensive spectroscopic analysis, and the structure of 2 was confirmed by X-ray diffraction analysis. A plausible biosynthetic pathway to 1 and 2 is proposed. Compound 1 was found to display dose-dependent inhibition of iNOS protein expression, and 1 and 2 were shown to possess significant neuroprotective activity.
Asunto(s)
Antozoos/química , Inhibidores de la Ciclooxigenasa 2/aislamiento & purificación , Sesquiterpenos/aislamiento & purificación , Animales , Cristalografía por Rayos X , Inhibidores de la Ciclooxigenasa 2/química , Inhibidores de la Ciclooxigenasa 2/farmacología , Lipopolisacáridos/farmacología , Macrófagos/efectos de los fármacos , Ratones , Conformación Molecular , Estructura Molecular , Óxido Nítrico/biosíntesis , Óxido Nítrico Sintasa de Tipo II/efectos de los fármacos , Resonancia Magnética Nuclear Biomolecular , Sesquiterpenos/química , Sesquiterpenos/farmacologíaRESUMEN
Two new cadinane-type sesquiterpenoids, scabralins A (1) and B (2) were obtained from the soft coral Sinularia scabra. Metabolite 1 was shown to exhibit moderate to weak cytotoxicity against MCF-7, WiDr, Daoy, and HEp 2 cancer cell lines. Also, incubation with 10 µM compound 1 significantly inhibited the accumulation of the pro-inflammatory inducible nitric oxide synthase protein in lipopolysaccharide-stimulated RAW264.7 macrophage cells.