RESUMEN
This study was conducted to assess the clinical effect of photodynamic therapy (PDT) in the decontamination of the deep dentin of deciduous molars submitted to partial removal of carious tissue. After cavity preparation, dentin samples were taken from the pulp wall of nineteen deciduous molars before and after PDT application. Remaining dentin was treated with 0.01% methylene blue dye followed by irradiation with an InGaAlP diode laser (λ - 660 nm; 40 mW; 120 J/cm2; 120 s). Dentin samples were microbiologically assessed for the enumeration of total microorganisms, Lactobacillus spp. and mutans streptococci. There was no significant difference in the number of colony-forming units (CFU) for any of the microorganisms assessed (p > 0.05). Photodynamic therapy, using 0.01% methylene blue dye at a dosimetry of 120 J/cm2 would not be a viable clinical alternative to reduce bacterial contamination in deep dentin.
Asunto(s)
Caries Dental/tratamiento farmacológico , Dentina/efectos de los fármacos , Inhibidores Enzimáticos/administración & dosificación , Azul de Metileno/administración & dosificación , Fotoquimioterapia/métodos , Diente Primario , Niño , Recuento de Colonia Microbiana , Dentina/microbiología , Dentina/efectos de la radiación , Femenino , Humanos , Lactobacillus/efectos de los fármacos , Lactobacillus/efectos de la radiación , Láseres de Semiconductores/uso terapéutico , Masculino , Estadísticas no Paramétricas , Streptococcus mutans/efectos de los fármacos , Streptococcus mutans/efectos de la radiación , Factores de Tiempo , Diente Primario/microbiología , Resultado del TratamientoRESUMEN
Abstract This study was conducted to assess the clinical effect of photodynamic therapy (PDT) in the decontamination of the deep dentin of deciduous molars submitted to partial removal of carious tissue. After cavity preparation, dentin samples were taken from the pulp wall of nineteen deciduous molars before and after PDT application. Remaining dentin was treated with 0.01% methylene blue dye followed by irradiation with an InGaAlP diode laser (λ - 660 nm; 40 mW; 120 J/cm2; 120 s). Dentin samples were microbiologically assessed for the enumeration of total microorganisms, Lactobacillus spp. and mutans streptococci. There was no significant difference in the number of colony-forming units (CFU) for any of the microorganisms assessed (p > 0.05). Photodynamic therapy, using 0.01% methylene blue dye at a dosimetry of 120 J/cm2 would not be a viable clinical alternative to reduce bacterial contamination in deep dentin.