RESUMEN
Acinetobacter baumannii is a ubiquitous microrganism often able to colonize and survive in different environments. Currently it is one of the most common pathogens responsible for nosocomial infections, including outbreaks, especially in long-term care facilities. The aim of this study was to show the results of an environmental investigation and genotyping analysis of multidrug-resistant Acinetobacter baumannii associated with an outbreak in an intensive care unit of a tertiary hospital located in Northern Sardinia, Italy. Positive cultures of MDR Acinetobacter baumannii were reported during the month of June 2012, after the collection of biological samples from ten patients. Acinetobacter baumannii was isolated during the following environmental investigation from the headboard of two beds. All the strains were genotyped by performing multiplex PCR to identify the presence of genes encoding carbapenemases. The results showed specific bands of bla(OXA-51-like) gene and of the bla(OXA-23-like) gene. PFGE highlighted minimal differences in genomic fingerprints, while the cluster analysis grouped the isolated microorganisms into two closely related clusters, characterized by Dice's similarity coefficient equal to 95.1%. MLST showed that the strains belonged to ST31. The results of the study highlight the need, especially in high-risk areas, to adopt strict hygiene practices, particularly hand hygiene, and to ensure an appropriate turnover of personal protective equipment, which could be responsible for the spread of biological agents, such as MDR Acinetobacter baumannii.
Asunto(s)
Infecciones por Acinetobacter/microbiología , Acinetobacter baumannii/efectos de los fármacos , Acinetobacter baumannii/aislamiento & purificación , Infección Hospitalaria/microbiología , Farmacorresistencia Bacteriana Múltiple , Equipos y Suministros de Hospitales/microbiología , Unidades de Cuidados Intensivos/estadística & datos numéricos , Infecciones por Acinetobacter/epidemiología , Acinetobacter baumannii/clasificación , Acinetobacter baumannii/genética , Infección Hospitalaria/epidemiología , Brotes de Enfermedades , Contaminación de Equipos/estadística & datos numéricos , Femenino , Humanos , Italia/epidemiología , Masculino , Centros de Atención Terciaria/estadística & datos numéricosRESUMEN
The aim of this study was to evaluate the antimicrobial activity of three absorbable, sterile, regenerated oxidized cellulose gauzes against ATCC and clinical isolates of bacterial and fungal strains, in particular those most frequently involved in surgical site infections. The three cellulose devices showed rapid antimicrobial activity against the microbial species tested. Their use could be a valuable adjunct to antibiotic prophylaxis in the prevention of surgical site infections.