RESUMEN
BACKGROUND/AIMS: Coronavirus (CoV) infections induce respiratory tract illnesses and central nervous system (CNS) diseases. We aimed to explore the cytokine expression profiles in hospitalized children with CoV-CNS and CoV-respiratory tract infections. METHODS: A total of 183 and 236 hospitalized children with acute encephalitis-like syndrome and respiratory tract infection, respectively, were screened for anti-CoV IgM antibodies. The expression profiles of multiple cytokines were determined in CoV-positive patients. RESULTS: Anti-CoV IgM antibodies were detected in 22/183 (12.02%) and 26/236 (11.02%) patients with acute encephalitis-like syndrome and respiratory tract infection, respectively. Cytokine analysis revealed that the level of serum granulocyte colony-stimulating factor (G-CSF) was significantly higher in both CoV-CNS and CoV-respiratory tract infection compared with healthy controls. Additionally, the serum level of granulocyte macrophage colony-stimulating factor (GM-CSF) was significantly higher in CoV-CNS infection than in CoV-respiratory tract infection. In patients with CoV-CNS infection, the levels of IL-6, IL-8, MCP-1, and GM-CSF were significantly higher in their cerebrospinal fluid samples than in matched serum samples. CONCLUSION: To the best of our knowledge, this is the first report showing a high incidence of CoV infection in hospitalized children, especially with CNS illness. The characteristic cytokine expression profiles in CoV infection indicate the importance of host immune response in disease progression.
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Enfermedades Virales del Sistema Nervioso Central/epidemiología , Infecciones por Coronavirus/epidemiología , Citocinas/sangre , Citocinas/líquido cefalorraquídeo , Infecciones del Sistema Respiratorio/epidemiología , Adolescente , Enfermedades Virales del Sistema Nervioso Central/diagnóstico , Enfermedades Virales del Sistema Nervioso Central/inmunología , Quimiocina CCL2/sangre , Quimiocina CCL2/líquido cefalorraquídeo , Niño , Niño Hospitalizado , Preescolar , China/epidemiología , Coronavirus/inmunología , Infecciones por Coronavirus/diagnóstico , Infecciones por Coronavirus/inmunología , Citocinas/inmunología , Progresión de la Enfermedad , Femenino , Factor Estimulante de Colonias de Granulocitos/sangre , Factor Estimulante de Colonias de Granulocitos/líquido cefalorraquídeo , Factor Estimulante de Colonias de Granulocitos y Macrófagos/sangre , Factor Estimulante de Colonias de Granulocitos y Macrófagos/líquido cefalorraquídeo , Humanos , Inmunoglobulina M/sangre , Inmunoglobulina M/líquido cefalorraquídeo , Lactante , Interleucina-6/sangre , Interleucina-6/líquido cefalorraquídeo , Interleucina-8/sangre , Interleucina-8/líquido cefalorraquídeo , Masculino , Infecciones del Sistema Respiratorio/diagnóstico , Infecciones del Sistema Respiratorio/inmunologíaRESUMEN
BACKGROUND: The 4-dose Essen intramuscular (IM) regimen for rabies post-exposure prophylaxis (PEP) has been recommended by Advisory Committee on Immunization Practices (ACIP) and World Health Organization (WHO), but the large-sample clinical evidence is still limited. METHOD: Rabies virus neutralizing antibodies of 11,752 patients were detected from 409 rabies prevention clinics in 27 provinces in China. Patients with serum collected before or no later than 1 h after injection on the day of the fifth dose (day 28) of 5-dose Essen regimen were included in Group A to observe the immune efficacy of 4-dose Essen IM regimen, and patients with serum collected 14-28 days after injection of the fifth dose were included in Group B to observe the immune efficacy of 5-dose Essen IM regimen. RESULTS: Finally, 2351 cases met the inclusion and exclusion criteria, including 2244 cases in Group A and 107 cases in Group B. The antibody titer of Group A was higher than that of Group B [12.21 (4.15, 32.10) IU/ml vs. 9.41 (3.87, 27.38) IU/ml] (P = 0.002). In Group A, the median antibody titers were 4.01IU/ml, 11.63IU/ml and 29.46IU/ml in patients vaccinated with purified hamster kidney cell vaccine (PHKCV), purified Vero cell vaccine (PVRV), and human diploid cell rabies vaccine (HDCV), respectively, with statistical significance (P < 0.001). CONCLUSIONS: The 4-dose Essen IM regimen could provide satisfactory immune effect, and HDCV induced higher antibody titer than PHKCV or PVRV.
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Anticuerpos Neutralizantes , Anticuerpos Antivirales , Profilaxis Posexposición , Vacunas Antirrábicas , Rabia , Humanos , Rabia/prevención & control , Vacunas Antirrábicas/inmunología , Vacunas Antirrábicas/administración & dosificación , Profilaxis Posexposición/métodos , China , Masculino , Inyecciones Intramusculares , Adulto , Femenino , Anticuerpos Antivirales/sangre , Estudios Transversales , Persona de Mediana Edad , Anticuerpos Neutralizantes/sangre , Virus de la Rabia/inmunología , Adolescente , Adulto Joven , Animales , Niño , Inmunogenicidad Vacunal , Esquemas de InmunizaciónRESUMEN
The currently recommended treatment for individuals exposed to rabies virus (RV) is post-exposure prophylaxis (PEP) through the combined administration of rabies vaccine and rabies immune globulin (RIG). Human monoclonal antibodies (mAbs) that neutralize RV offer an opportunity to replace RIG for rabies PEP. Here, a combinatorial human Fab library was constructed using antibody genes derived from the blood of RV-vaccinated donors. Selections of this library against purified RV virions resulted in the identification of 11 unique Fab antibodies specific for RV glycoprotein. Of the Fab antibodies, five were converted to full human IgG1 format. The human IgG antibodies revealed high binding affinity and neutralizing activities against RV fixed strains through a rapid fluorescent focus inhibition test in vitro as well as the early stage protective function after exposure to RV infection in vivo. Furthermore, epitope mapping and binding competition analysis showed that all of obtained human neutralizing and protective antibodies were directed to the antigenic site II of RV glycoprotein. Our results provide not only important insight into the protective immune response to RV in humans, but also more candidates eligible for use in a mAb cocktail aimed at replacing RIG for rabies post-exposure prophylaxis.
Asunto(s)
Anticuerpos Neutralizantes/genética , Anticuerpos Antivirales/inmunología , Glicoproteínas/inmunología , Virus de la Rabia/inmunología , Rabia/tratamiento farmacológico , Proteínas Virales/inmunología , Animales , Anticuerpos Neutralizantes/inmunología , Anticuerpos Neutralizantes/uso terapéutico , Anticuerpos Antivirales/genética , Anticuerpos Antivirales/uso terapéutico , Cricetinae , Glicoproteínas/genética , Humanos , Fragmentos Fab de Inmunoglobulinas/genética , Fragmentos Fab de Inmunoglobulinas/inmunología , Fragmentos Fab de Inmunoglobulinas/uso terapéutico , Inmunoglobulina G/genética , Inmunoglobulina G/inmunología , Inmunoglobulina G/uso terapéutico , Mesocricetus , Profilaxis Posexposición , Rabia/inmunología , Rabia/prevención & control , Rabia/virología , Virus de la Rabia/genética , Proteínas Virales/genéticaRESUMEN
Smart surfaces promote the fundamental understanding of wetting and are widely used in practical applications for energy and water collection. Light-induced switchable wettability facilitated by ZnO coatings, for instance, was developed for liquid manipulation at the surface. However, the transition of wetting states was reported to follow a hydrophobic-hydrophilic cycle in an hour, which is very long and may limit its future applications. We recently discovered that the cycle of the wetting state transitions on inorganic coatings can be shortened to less than 100 seconds by using ALD-coated ZnO on a pillared surface. However, the mechanisms are still unclear. Here, we investigated the effects of coating thickness on the transition speed and found that it significantly depended on the thickness of the coating with the optimal thickness less than 50 nm. We found that the minimum critical time for a wetting state transition cycle was less than 50 seconds with a thickness of 40 nm. Although the transition time of surfaces with coatings over 70 nm thickness remained constant at 10 min for a cycle, it was shorter than those of other deposition techniques for a coarse surface. Here, we propose a "penetration-diffusion" model to explain the fast and thickness-dependent wetting transitions. Our study may provide a new paradigm for fast wetting transition surfaces with cycle time within tens of seconds using a homogeneous thin layer coated on a rough surface.
RESUMEN
Photo-corrosion is a common phenomenon observed in the photocatalytic semiconductor materials, which can seriously harm the photoelectric properties and performances in the energy applications. However, in this paper, we demonstrated that the photo-corrosion effects can be used for the microfabrication of conductive structures on a photocatalytic film like zinc oxide (ZnO), named as "photoetching". Our results demonstrated that microstructures can be prepared within seconds with a precision at an order of tens of micrometers using our current devices. Different from the previous work, the etching process was achieved free of conducting layer under the ZnO film, avoiding the short-circuit of the conductive micro-patterns and enabling the use for the impedance sensing. We demonstrated the fabricated ZnO microelectrode pairs can work for the electrochemical impedance measurements like assessment of hemostasis integrated with a microfluidic chip. Compared to the noble metal microelectrodes, the ZnO conductive microelectrodes can be fabricated within seconds and the low costs make it possible as a disposable diagnostic device. Besides, the photoetching technique can be performed without a cleanroom reducing the technical barriers, possibly helpful for the low resources areas. We believe the simplicity of device, low costs and fast fabrication can be useful in the relevant fields such as biomedical and energy harvesting, especially for low resources areas.
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Técnicas Biosensibles/instrumentación , Oro/química , Hemostasis , Microtecnología/métodos , Óxido de Zinc/química , Pruebas de Coagulación Sanguínea/instrumentación , Conductividad Eléctrica , Diseño de Equipo , Humanos , Microelectrodos , Microtecnología/instrumentaciónRESUMEN
BACKGROUND: Rabies is a serious reemerging zoonosis in China. At present human rabies cases are primarily diagnosed based on clinical presentation. CASE PRESENTATION: In August 2012, a woman and her son were attacked by a stray dog in Henan, China. The son received rabies postexposure prophylaxis (wound treatment followed by vaccine, no immunoglobulin), however, the mother did not. Rabies infection was subsequently laboratory confirmed in the mother and she died in December; her son is alive and healthy after 2 years of follow-up. CONCLUSION: This report documents that the timely utilization of postexposure prophylaxis is a required measure in preventing rabies after exposure to an animal bite.
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Mordeduras y Picaduras/etiología , Profilaxis Posexposición/estadística & datos numéricos , Vacunas Antirrábicas/administración & dosificación , Virus de la Rabia/fisiología , Rabia/tratamiento farmacológico , Adulto , Animales , Niño , China , Enfermedades de los Perros/virología , Perros , Resultado Fatal , Femenino , Humanos , Masculino , Rabia/inmunología , Virus de la Rabia/genéticaRESUMEN
China has the second highest number of rabies cases worldwide. There are millions of category III rabies exposure cases in China every year, which are treated with rabies immunoglobulin(RIG)and the rabies vaccine. The price of RIG is relatively expensive, and the application of RIG is relatively complicated. The rate of RIG use in post exposure prophylaxis(PEP)for rabies exposure category â ¢ cases has remained low for a significant amount of time. Reducing the dosage of RIG could reduce the cost of PEP, while simplifying the use of RIG could make PEP easier. Together, these steps could improve the rate of RIG utilization in PEP. There are conflicting conclusions in studies of RIG on the immune effects of the rabies vaccine.Exploring the mechanism of action of RIG in the immune response to the rabies vaccine would help to explain the role of RIG in the immune effects mediated by the rabies vaccine. In this paper, the progress of research on the application of RIG is systematically reviewed in order to provide a reference for the formulation of new and more practical guidelines for the application of RIG.
Asunto(s)
Inmunoglobulina G/uso terapéutico , Rabia/prevención & control , Animales , China , Humanos , Inmunoglobulina G/inmunología , Profilaxis Posexposición/tendencias , Rabia/inmunología , Rabia/virologíaRESUMEN
To study the B cell linear epitopes of rabies virus CVS-11 nucleoprotein, peptides were synthesized according to the amino acid sequences of B cell linear epitopes. Linear epitopes predicted by bioinformatics analysis were evaluated with immunological techniques. Indirect enzyme-linked immunosorbent assay showed that titers of antibodies to peptides (355-369 and 385-400 residues of rabies virus CVS-11 nucleoprotein) were above 1:12 800 in mouse sera. The antibodies recognized denatured rabies virus CVS-11 nucleoprotein in Western blot analysis. Purified anti-peptide antibodies recognized natural rabies virus CVS-11 nucleoprotein in BHK-21 cells in indirect fluorescent antibody test. The 355-369 and 385-400 residues of rabies virus CVS-11 nucleoprotein were validated as B cell linear epitopes.
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Epítopos de Linfocito B/química , Epítopos de Linfocito B/inmunología , Nucleoproteínas/química , Nucleoproteínas/inmunología , Virus de la Rabia/inmunología , Rabia/virología , Secuencia de Aminoácidos , Animales , Anticuerpos Antivirales/inmunología , Mapeo Epitopo , Epítopos de Linfocito B/genética , Femenino , Humanos , Masculino , Ratones , Ratones Endogámicos BALB C , Datos de Secuencia Molecular , Nucleoproteínas/genética , Rabia/inmunología , Virus de la Rabia/química , Virus de la Rabia/genéticaRESUMEN
The World Health Organization (WHO) standard assay for determining levels of the rabies virus neutralization antibody (RVNA) is the rapid fluorescent focus inhibition test (RFFIT), which is used to evaluate the immunity effect after vaccination against rabies. For RFFIT, CVS-11 was used as the challenge virus, BSR cells as the adapted cells, and WHO rabies immunoglobulin (WHO STD) as the reference serum in this study. With reference to WHO and Pasteur RFFIT procedures, a micro-RFFIT procedure adapted to our laboratory was produced, and its specificity and reproducibility were tested. We tested levels of RVNA in human serum samples after immunization with different human rabies vaccines (domestic purified Vero cell rabies vaccine (PVRV) and imported purified chick embryo cell vaccine (PCECV)) using different regimens (Zagreb regimen and Essen regimen). We analyzed the levels of RVNA, and compared the immune efficacy of domestic PVRV and imported PCECV using different immunization regimens. The results showed that the immune efficacy of domestic PVRV using the Zagreb regimen was as good as that of the imported PCECV, but virus antibodies were generated more rapidly with the Zagreb regimen than with the Essen regimen. The RFFIT procedure established in our laboratory will enhance the comprehensive detection ability of institutions involved in rabies surveillance in China.
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Anticuerpos Neutralizantes/sangre , Anticuerpos Antivirales/sangre , Pruebas de Neutralización/métodos , Vacunas Antirrábicas/inmunología , Virus de la Rabia/inmunología , Virología/métodos , China , Fluorescencia , Humanos , Reproducibilidad de los Resultados , Sensibilidad y EspecificidadRESUMEN
BACKGROUND: The family Tymoviridae comprises three plant virus genera, including Tymovirus, Marafivirus, and Maculavirus, which are found in most parts of the world and cause severe agricultural losses. We describe a putatively novel member of the family Tymoviridae, which is isolated from mosquitoes (Culex spp.), referred to as CuTLV. METHODS AND RESULTS: The CuTLV was isolated by cell culture, which replicates and causes cytopathic effects in Aedes albopictus C6/36 cells, but not in mammalian BHK-21 or Vero cells. The complete 6471 nucleotide sequence of CuTLV was determined. The genome of CuTLV is predicted to contain three open reading frames (ORFs). The largest ORF1 is 5307 nucleotides (nt) in length and encodes a putative polypeptide of 1769 amino acids (aa), which contains the conserved motifs for the methyltransferase (MTR), Tymovirus endopeptidase (PRO), helicase (HEL), and RNA-dependent RNA polymerase (RdRp) of the replication-associated proteins (RPs) of positive-stranded RNA viruses. In contrast, the ORF1 sequence does not contain the so-called "tymobox" or "marafibox", the conserved subgenomic RNA promoter present in all tymoviruses or marafiviruses, respectively. ORF2 and ORF3 putatively encode a 248-aa coat protein (CP) and a proline-rich 149-aa polypeptide. The whole genomic nucleotide identity of CuTLV with other members of family Tymoviridae ranged from 46.2% (ChiYMV) to 52.4% (GFkV). Phylogenetic analysis based on the putative RP and CP genes of CuTLV demonstrated that the virus is most closely related to viruses in the genus Maculavirus. CONCLUSIONS: The CuTLV is a novel virus related to members of the family Tymoviridae, with molecular characters that are distinct from those of tymoviruses, marafiviruses, and other maculaviruses or macula-like viruses. This is the first report of the isolation of a Tymoviridae-like virus from mosquitoes. Further investigations are required to clarify the origin, replication strategy, and the public health or agricultural importance of the CuTLV.
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Culex/virología , Genoma Viral/fisiología , Sistemas de Lectura Abierta/fisiología , Tymoviridae , Proteínas Virales , Animales , Línea Celular , Cricetinae , Tymoviridae/clasificación , Tymoviridae/genética , Tymoviridae/aislamiento & purificación , Tymoviridae/metabolismo , Proteínas Virales/genética , Proteínas Virales/metabolismoRESUMEN
Liao ning virus (LNV) is related to Banna virus, a known human-pathogen present in south-east Asia. Both viruses belong to the genus Seadornavirus, family Reoviridae. LNV causes lethal haemorrhage in experimentally infected mice. Twenty seven isolates of LNV were made from mosquitoes collected in different locations within the Xinjiang province of north-western China during 2005. These mosquitoes were caught in the accommodation of human patients with febrile manifestations, or in animal barns where sheep represent the main livestock species. The regions where LNV was isolated are affected by seasonal encephalitis, but are free of Japanese encephalitis (JE). Genome segment 10 (Seg-10) (encoding cell-attachment and serotype-determining protein VP10) and Seg-12 (encoding non-structural protein VP12) were sequenced for multiple LNV isolates. Phylogenetic analyses showed a less homogenous Seg-10 gene pool, as compared to segment 12. However, all of these isolates appear to belong to LNV type-1. These data suggest a relatively recent introduction of LNV into Xinjiang province, with substitution rates for LNV Seg-10 and Seg-12, respectively, of 2.29×10(-4) and 1.57×10(-4) substitutions/nt/year. These substitution rates are similar to those estimated for other dsRNA viruses. Our data indicate that the history of LNV is characterized by a lack of demographic fluctuations. However, a decline in the LNV population in the late 1980s-early 1990s, was indicated by data for both Seg-10 and Seg-12. Data also suggest a beginning of an expansion in the late 1990s as inferred from Seg-12 skyline plot.
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Culicidae/virología , Modelos Moleculares , Filogenia , Reoviridae/genética , Proteínas Virales/química , Animales , Secuencia de Bases , Teorema de Bayes , China , Análisis por Conglomerados , Biología Computacional , Cartilla de ADN/genética , Ensayo de Inmunoadsorción Enzimática , Ratones , Modelos Genéticos , Datos de Secuencia Molecular , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa , Análisis de Secuencia de ADN , Especificidad de la Especie , Proteínas Virales/genéticaRESUMEN
OBJECTIVE: Compare the difference of the results referred to the WHO standard rabies immunoglobulin and the national standard human rabies immunoglobulin used in the rapid fluorescent focus inhibition test (RFFIT). METHODS: Setting the WHO standard immunoglobulin and the national standard immunoglobulin in the same system and testing 12 human serum at the same time. Compare the fluorescence percentage of the two different standard immunoglobulin; compare the 12 serum results calculated from the two different standard immunoglobulin used the calculation formula of neutralization antibody titer. RESULTS: The Results display that the 50% percent of the two standard immunoglobulin are all between the fifth and the sixth well, but the percentage of the national standard immunoglobulin is lower than the WHO one. The same testserum result calculated from the WHO standard immunoglobulin is little higher than the national one. CONCLUSION: There is difference in the WHO standard immunoglobulin and the national one, but there is no influence in the results.
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Técnica del Anticuerpo Fluorescente/métodos , Inmunoglobulinas/inmunología , Pruebas de Neutralización/métodos , Rabia/diagnóstico , Técnica del Anticuerpo Fluorescente/normas , Humanos , Pruebas de Neutralización/normas , Rabia/inmunología , Virus de la Rabia/inmunología , Estándares de Referencia , Organización Mundial de la SaludRESUMEN
A combinatorial human Fab library to the rabies virus was constructed using antibody genes derived from the blood of vaccinated donors. The library were panned and selected on purified rabies virus particles of aG or CTN strain with phage display. Eleven unique human Fab antibodies specific for the rabies virus glycoprotein were obtained by ELISA, IFA and DNA sequences analysis of these antibodies. Among these Fab antibodies, five human Fab antibodies were converted to full-length human IgG antibodies with recombinant baculovirus system. The five full-length human IgG antibodies were tested in vitro for rabies virus neutralization, resulting in all specificities to neutralize the virus. The obtained human anti-rabies antibodies lay the basis for the production of cocktail of anti-rabies monoclonal antibody with chinese intellectual property.
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Anticuerpos Neutralizantes/inmunología , Anticuerpos Antivirales/inmunología , Fragmentos Fab de Inmunoglobulinas/inmunología , Virus de la Rabia/inmunología , Rabia/inmunología , Secuencia de Aminoácidos , Animales , Línea Celular , Cricetinae , Humanos , Fragmentos Fab de Inmunoglobulinas/genética , Inmunoglobulina G/genética , Inmunoglobulina G/inmunología , Datos de Secuencia Molecular , Pruebas de Neutralización , Rabia/virología , Virus de la Rabia/genéticaRESUMEN
OBJECTIVE: To establish a rapid fluorescent inhibition test (RFFIT) for testing rabies virus neutralizing antibody and the titer of rabies virus neutralizing antibody. CVS-11 was used as the standard challenge virus, and three generations prepared for the establishment of the virus library. METHODS: International standard for rabies immunoglobulin was used as the reference serum. RFFIT test was established under consulting the protocol of Institute of Pasteur, and its specificity, stability and reproducibility were validated. RESULTS: We established the RFFIT which showed both good specificity (100%) and reproducibility (P > 0.5). CONCLUSION: The establishment of RFFIT test perfected the rabies laboratory techniques and would enhance the overall ability in detecting rabies in China.
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Anticuerpos Neutralizantes/sangre , Anticuerpos Antivirales/sangre , Técnica del Anticuerpo Fluorescente/métodos , Virus de la Rabia/aislamiento & purificación , Humanos , Pruebas de Neutralización/métodos , Virus de la Rabia/inmunologíaRESUMEN
5 strains of virus isolated from Culex tritaeniorhynchus, Anopheles sinensis and Armigeres subalbatus, which caused cytopathic effect in C6/36 cells, had been obtained in the survey of arboviruses in Northwestern Yunnan Province. China. The virus particles displayed 70 nanometers diameter (n=7) with no envelope but spikes on the surfaces. RNA-PAGE of the genomes of the isolates showed 6-5-1 profile. A fragment of the 12th segment sequence was amplified by a pair of specific primers for Kadipiro virus strain JKT-7075 in RT-PCR. The full length of the 12th segment was 758 nucleotides, BLAST analysis revealed the highest identity was 90% to JKT-7075. Phylogenetic analysis demonstrated that the isolates appeared to be Kadipiro viruses (Family Reoviridae). It was the first report of kadipiro virus isolation in China.
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Coltivirus/clasificación , Coltivirus/aislamiento & purificación , Secuencia de Aminoácidos , Animales , Anopheles/virología , Línea Celular , China , Coltivirus/genética , Culex/virología , Datos de Secuencia Molecular , Filogenia , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa , Análisis de Secuencia de ADNRESUMEN
During an investigation of arboviruses in China, a novel densovirus (DNV) was isolated from the adult female Culex pipiens pallens. The virus, designated Culex pipiens pallens densovirus (CppDNV), caused cytopathic effect in C6/36 cells. The virus particles were icosahedral, non-enveloped and had a mean diameter of 24 nm. The complete coding region of CppDNV was found to be 3335 nt and it contained three open reading frames (ORFs). CppDNV shares 82-93 % identical nucleotides with isolates of the Aedes albopictus densovirus [isolates AalDNV-1, AalDNV-2 (C6/36 DNV) and AalDNV-3], Aedes aegypti densovirus (AaeDNV) and Haemagogus equines densovirus (HeDNV). The nucleotide sequence identity among CppDNV isolates exceeds 98 %. Phylogenetic trees based on non-structural (NS1 and NS2) and capsid (VP) genes show that CppDNV clustered with the species AaeDNV and represents a novel variant of this species within the genus Brevidensovirus.
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Culex/virología , Densovirus/clasificación , Densovirus/genética , Secuencia de Aminoácidos , Animales , Secuencia de Bases , China , Secuencia Conservada , Cartilla de ADN , ADN Viral/genética , Densovirus/aislamiento & purificación , Datos de Secuencia Molecular , FilogeniaRESUMEN
0507BS3 virus was isolated from a mixed pool of Culex sp. and Anopheles sp. collected in Kashi, Xinjiang, China. 0507BS3 virus could cause cytopathic effects on C6/36 cells but not on Vero and BHK-21 cells. Viral particles had no envelope and appeared round with diameter of about 60 nm (n = 20). Viral capsid was composed of a single layer and a central core. Capsomeres on the surface of capsid were clearly visible. Electrophoresis of viral genome showed a profile of 10 double stranded RNA (dsRNA) segments. Sequencing of the tenth segment revealed the length of 964bp (GenBank ID: FJ150869). A single open reading frame (ORF) was found and encoded a protein of 275 amino acids with a molecular mass of 30.8kDa. The nucleotide sequence had no similarity with any other viral genomic sequences, but the deduced amino acid sequence significantly matched the polyhedrin genes of cytoplasmic polyhedrosis virus (CPV) in some sections. A phylogenetic tree was constructed to compare the polyhedrin gene sequences of all CPV types in GenBank. The tree demonstrated that 0507BS3 virus was only distantly related to the other CPV types and belonged to a new CPV type.
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Culicidae/virología , Reoviridae/aislamiento & purificación , Animales , Línea Celular , China , Filogenia , Reoviridae/clasificación , Reoviridae/genética , Reoviridae/ultraestructuraRESUMEN
0507JS60 virus was isolated from a pool of Culex sp. collected in Kashi, Xinjiang, which could be propagated stably on C6/36 cells and caused cytopathic effects continuously. Viral particles had no envelope and appeared round with diameter of about 55nm (n = 10). Capsomeres on the surface of capsid were clearly visible. Electrophoresis of viral genome showed a profile of 12 double stranded RNA (dsRNA) segments. Sequencing of the twelfth segment revealed the length of 760bp (GenBank ID: FJ157354). A single open reading frame (ORF) was found and encoded a protein of 174 amino acids with a molecular mass of 18.9kD. The nucleotide sequence had similarity over 89% with that of LNV, but the deduced amino acid sequence had similarity over 91% with that of LNV. A phylogenetic tree was constructed to compare the corresponding genetic sequences in Seadornavirus. The tree demonstrated that 0507JS60 virus lied in the same branch with LNV and more closely related to LNV-NE9712. 0507JS60 virus was identified as LNV, which was firstly isolated outside the Northeast of China.
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Culex/virología , Reoviridae/clasificación , Reoviridae/aislamiento & purificación , Animales , Línea Celular , China , Datos de Secuencia Molecular , Filogenia , Reoviridae/genética , Reoviridae/ultraestructuraRESUMEN
OBJECTIVE: To study the clinical and laboratory characteristics of adult Japanese encephalitis (JE) patients in a JE outbreak in Yuncheng, Shanxi Province in 2006. METHOD: All the clinical data from the Second People's Hospital in Yuncheng city were analyzed, part of patients' sera and cerebrospinal fluid were tested by serology and RT-PCR. RESULTS: The majority of patients were middle-aged and elderly, 77.8% (35/45) of the total cases were more than 40 years old. Severe and fulminating type cases accounted for 60.0% (27/45). Most patients had underlying diseases. IgM antibody to JE virus (JEV) in serum was positive in each of the 45 patients analyzed and 4-fold or greater rise in sera neutralization antibody titer were found in convalescent serum. JEV nucleic acid was positive in part of cerebrospinal fluid specimens. CONCLUSION: Viral encephalitis emerged in Yuncheng city, Shanxi Province was Japanese encephalitis B, and most of the cases belonged to elderly group.
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Brotes de Enfermedades , Virus de la Encefalitis Japonesa (Especie)/aislamiento & purificación , Encefalitis Japonesa/epidemiología , Adolescente , Adulto , Anciano , Anciano de 80 o más Años , Anticuerpos Antivirales/sangre , Niño , Preescolar , China/epidemiología , Virus de la Encefalitis Japonesa (Especie)/genética , Virus de la Encefalitis Japonesa (Especie)/inmunología , Encefalitis Japonesa/sangre , Encefalitis Japonesa/líquido cefalorraquídeo , Femenino , Humanos , Inmunoglobulina M/sangre , Masculino , Persona de Mediana Edad , Pruebas de Neutralización , ARN Viral/genética , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa , Adulto JovenRESUMEN
BACKGROUND: To study the arboviruses carried by mosquitoes collected in Hebei Province. METHODS: Samples were collected from mosquito active sites and stored in liquid nitrogen till use. Pools of 20 to 30 mosquitoes were ground after sterilization, centrifugal supernant was inoculated onto C6/36 cell, cytopathic effect was observed for three sequential passages. Positive isolates were identified by IFA and RT-PCR. RESULTS: Totally 1310 mosquitoes were collected from two villages of She county, Hebei province. They were divided into 46 pools and ground respectively. Thirteen positive isolates were obtained. Two isolates reacted with alphaviral antibodies and were amplified by alphaviral primers, nucleotide sequence showed the highest homology (98%) to Getah virus (AY702913.1), so the two isolates were identified as Getah virus. CONCLUSION: Getah virus was isolated from mosquitoes in Hebei Province. This is the first report of isolating Getah virus from inland of China.