RESUMEN
Fatty liver is one of the most pervasive liver diseases worldwide. Probiotics play an important role in the progression of liver disease, but their effects on host regulation are poorly understood. This study investigated the protective effects of lactobacillus gasseri (L. gasseri) against high-cholesterol diet (HCD)-induced fatty liver injury using a zebrafish larvae model. Liver pathology, lipid accumulation, oxidative stress and hepatic inflammation were evaluated to demonstrate the changes in a spectrum of hepatic injury. Moreover, multiple indexes on host gene expression profiles were comprehensively characterized by RNA screening. The results showed that treatment with L. gasseri ameliorated HCD-induced morphological and histological alterations, lipid regulations, oxidative stress and macrophage aggregation in the liver of zebrafish larvae. Furthermore, the enrichment of the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway revealed that the core pathways of L. gasseri regulation were interleukin-17 (IL-17) signaling, phosphoinositide 3-kinase (PI3K)-AKT signaling pathway, the regulation of lipolysis and adipocytes and fatty acid elongation and estrogen signaling. The genes at key junction nodes, hsp90aa1.1, kyat3, hsd17b7, irs2a, myl9b, ptgs2b, cdk21 and papss2a were significantly regulated by L. gasseri administration. To conclude, the current research extends our understanding of the protective effects of L. gasseri against fatty liver and provides potential therapeutic options for fatty liver treatment.
Asunto(s)
Dieta Alta en Grasa , Hígado Graso , Lactobacillus gasseri , Probióticos , Pez Cebra , Animales , Colesterol/análisis , Colesterol/metabolismo , Dieta/efectos adversos , Hígado Graso/etiología , Hígado Graso/genética , Hígado Graso/metabolismo , Hígado Graso/prevención & control , Lactobacillus gasseri/metabolismo , Lípidos/farmacología , Hígado/efectos de los fármacos , Hígado/metabolismo , Fosfatidilinositol 3-Quinasas/metabolismo , Probióticos/farmacología , Probióticos/uso terapéutico , Transcriptoma , Pez Cebra/metabolismo , Perfilación de la Expresión Génica , Regulación de la Expresión Génica , Larva/genéticaRESUMEN
The mechanical signals within the extracellular matrix (ECM) regulate cell growth, proliferation and differentiation, and integrins function as the hub between the ECM and cellular actin. Focal adhesions (FAs) are multiprotein, integrincontaining complexes, acting as tensionsensing anchoring points that bond cells to the extracellular microenvironment. Talin1 serves as the central protein of FAs that participates in the activation of integrins and connects them with the actin cytoskeleton. As a cytoplasmic protein, Talin1 consists of a globular head domain and a long rod comprised of a series of αhelical bundles. The unique structure of the Talin1 rod domain permits folding and unfolding in response to the mechanical stress, revealing various binding sites. Thus, conformation changes of the Talin1 rod domain enable the cell to convert mechanical signals into chemical through multiple signaling pathways. The present review discusses the binding partners of Talin1, their interactions, effects on the cellular processes, and their possible roles in diseases.