MiR-26b-5p inhibits cell proliferation and EMT by targeting MYCBP in triple-negative breast cancer.

BACKGROUND: The study was designed to elucidate the association and functional roles of miR-26b-5p and c-MYC binding protein (MYCBP) in triple-negative breast cancer (TNBC). METHOD: Luciferase reporter assay was used to confirm the relationship between miR-26b-5p and MYCBP in TNBC cells. The expression levels of miR-26b-5p and MYCBP in tissue specimens and cell lines were determined using reverse transcription-quantitative PCR. Cell proliferation, migration and invasion were assessed using CCK-8 assay, colony formation and transwell assay. RESULTS: We first observed that miR-26b-5p directly targets the 3'-UTR of MYCBP to inhibit MYCBP expression in MDA-MB-468 and BT-549 cells. The expression of miR-26b-5p was inversely correlated with MYCBP expression in TNBC tissues. We further demonstrated that MYCBP knockdown suppressed the proliferation, migration and invasion of TNBC cells. Furthermore, MYCBP overexpression counteracted the suppressive effect of miR-26b-5p on TNBC cell behaviors. Western blot analysis demonstrated that the E-cadherin protein level was increased, while protein levels of N-cadherin and vimentin were decreased in cells transfected with miR-26b-5p, which were all reversed by ectopic expression of MYCBP. CONCLUSIONS: In summary, our findings revealed the tumor suppressive role of miR-26b-5p in regulating TNBC cell proliferation and mobility, possibly by targeting MYCBP.

miR-199a-5p inhibits the progression of papillary thyroid carcinoma by targeting SNAI1.

BACKGROUND: Increasing evidence has emphasized the important roles of differentially expressed miRNAs in papillary thyroid cancer (PTC) development. miR-199a-5p was previously documented to be downregulated in PTCs compared with normal thyroids. However, the role of miR-199a-5p in the progression of PTC and the underlying mechanism remain to be further addressed. METHODS: miR-199a-5p and snail family zinc finger 1 (SNAI1) mRNA expressions in PTC tissues and cells were detected by qRT-PCR. The effects of miR-199a-5p and SNAI1 on cell migration, invasion and epithelial-mesenchymal transition (EMT) were evaluated by cell migration and invasion assays, and western blot, respectively. The relationship between miR-199a-5p and SNAI1 was investigated by luciferase reporter assay and western blot. Xenograft tumor assay was performed to verify the role of miR-199a-5p and molecular mechanism in PTC. RESULTS: miR-199a-5p expression was significantly downregulated and SNAI1 was markedly upregulated in PTC tissues and cells. miR-199a-5p overexpression and SNAI1 knockdown suppressed the progression of PTC cells in vitro, as evidenced by the reduced cell migration, invasion and EMT. Of note, SNAI1 was identified as a target of miR-199a-5p and miR-199a-5p suppressed SNAI1 expression in PTC cells. Xenograft tumor assay proved that miR-199a-5p overexpression suppressed tumor growth in PTC in vivo by downregulating SNAI1. CONCLUSION: miR-199a-5p inhibited the progression of PTC by downregulating SNAI1, offering new insight into the molecular mechanism underlying PTC progression.

Chlorination behaviors for green and efficient vanadium recovery from tailing of refining crude titanium tetrachloride.

The refined tailing, generated from refining of titanium tetrachloride (TiCl4) for vanadium (V) removal, is a hazardous material to environment due to the high content of V. Aiming at effective and selective extraction of V from the refined tailing, a fluidized chlorination process was proposed in present work. The chlorination behaviors of the refined tailing which determine the efficiency and selectivity of V extraction were emphatically investigated. A resultant 96.36% of V and 4.23% of Ti can be synchronously extracted from the tailing at the optimum conditions of 800 °C for 60 min, with the pressure fraction of chlorine [P(Cl2)/P(Cl2 +N2)] = 0.5 and the mass fraction of petroleum coke in raw materials for chlorination at 10 wt%. High purity vanadium oxytrichloride (VOCl3, higher than 99.99 wt%) can be finally obtained via further simple purification of the collected chloride product. Moreover, the chlorination residue containing concentrated TiO2 has the potential to be further utilized for Ti extraction. Thus the process provides a new prospect for effective, clean and comprehensive utilization of the refined tailing, which can solve the hazardous waste recycle and environmental concerns simultaneously.

Association of tea consumption and the risk of thyroid cancer: a meta-analysis.

OBJECTIVES: Epidemiological studies evaluating the association of tea consumption and the risk of thyroid cancer risk have produced inconsistent results. Thus, we conducted a meta-analysis to assess the relationship between tea consumption and thyroid cancer risk. METHODS: Pertinent studies were identified by a search in PubMed and Web of Knowledge. The random effect model was used based to combine the results. Publication bias was estimated using Egger's regression asymmetry test. RESULTS: Finally, 11 articles with 14 studies (2 cohort studies and 12 case-control studies) involving 2,955 thyroid cancer cases and 106,447 participants were included in this meta-analysis. The relative risk (95% confidence interval) of thyroid cancer for the highest versus the lowest category of tea consumption was 0.774 (95% CI = 0.619-0.967), and the associations were also significant in Europe and America, but not in the Asia. No publication bias was found. CONCLUSIONS: Our analysis indicated that higher tea consumption may have a protective effect on thyroid cancer, especially in Europe and America.