Feasibility of Digital Image Correlation for Fatigue Cracks Detection under Dynamic Loading.

We address non-contact detection of defects in the railway rails under their dynamic loading and propose to combine digital image correlation (DIC) and finite element modeling (FEM). We show that accurate model of defect-free rail operating at the same loading conditions as the inspected one provides a reliable reference for experimental data. In this study, we tested the rail samples with artificial and fatigue defects under cyclic loading, calculated displacement and stress distributions at different locations of the cracks via DIC and validated the obtained results by FEM. The proposed DIC-FEM approach demonstrates high sensitivity to fatigue cracks and can be effectively used for remote control of rails as well as for non-destructive testing of various other objects operating under dynamic loads.

Optimization of prism-based stereoscopic imaging systems at the optical design stage with respect to required 3D measurement accuracy.

We address the optical design procedure of prism-based stereoscopic imaging systems. Conventional approach includes two sequential stages: selection of the hardware and development of the proper digital image processing algorithms. At each of these stages, specific techniques are applied, which are almost unrelated to each other. The main requirements to the imaging system include only the key parameters and the image quality. Therefore, the insufficient measurement accuracy may be revealed only after the prototype is assembled and tested. In this case, even applying complex time-consuming image processing and calibration procedures does not ensure the necessary precision. A radical solution of this issue is to include the measurement error estimation into the optical design stage. In this research, we discuss a simplified implementation of this approach and demonstrate the capabilities of optical design software for this purpose. We demonstrate the effectiveness of this approach by the analysis and optimization of a prism-based stereoscopic imager with respect to required 3D measurement accuracy. The results are meaningful for the development of 3D imaging techniques for machine vision, endoscopic and measurement systems.

Optimization of stereoscopic imager performance by computer simulation of geometrical calibration using optical design software.

Stereoscopic imagers are widely used in machine vision for three-dimensional (3D) visualization as well as in non-destructive testing for quantitative characterization of cracks, delamination and other defects. Measurement capability in these systems is provided by a proper combination of the optical parameters and data processing techniques. Conventional approach to their design consists of two sequential stages: optical system design and optimization of calibration and image processing algorithms. Such two-stage procedure often complicates both the hardware and the software, and results in a time-ineffective design procedure and cost-ineffective solution. We demonstrate a more effective approach and propose to estimate errors of 3D measurements at the early optical design stage. We show that computer simulation using optical design software allows not only optimizing optical parameters of the imager but also choosing the most effective mathematical model of the system and the equipment necessary for calibration. We tested the proposed approach on the design of miniature prism-based stereoscopic system and analyzed the impact of various factors (aberrations, tolerances, etc.) as on the image quality, so on the quality of calibration and 3D measurements accuracy. The proposed joint design approach may be highly effective for various measurement systems and applications when both optical parameters and image processing algorithms are not defined in advance and are necessary to be optimized.

Modeling and optimization of a geometrical calibration procedure for stereoscopic video endoscopes.

Stereoscopic video endoscopes are widely used for remote visual inspection and precise three-dimensional (3D) measurements in industrial and biomedical applications. The reconstruction of 3D points from the corresponding image points requires a geometrical calibration procedure, the accuracy of which affects the measurement uncertainty. We propose to perform an optimal choice of the calibration technique and the calibration target parameters using a computer simulation at the design stage. The effectiveness of this approach is demonstrated via the design of a self-developed miniature prism-based stereoscopic system. We simulated acquisition of calibration and measurement data using optical design software. The conventional calibration technique, requiring many positions of the flat target with arbitrary displacements and rotations, was compared with another one that uses the translation stage to provide pure translation of the target. We analyzed the impact of the translation uncertainty, the number of positions, the number of targets, and the uncertainty of image point coordinates on the uncertainty of calibration parameters and 3D measurements. We have shown that the second technique could provide acceptable measurement accuracy using only two images. The results of the computer simulation were confirmed experimentally using the prototype of the stereoscopic endoscope. The proposed approach may be used to optimize calibration techniques and reduce the cost of calibration equipment for various stereoscopic measurement systems.

Imaging system based on a tandem acousto-optical tunable filter for in situ measurements of the high temperature distribution.

We present, to the best of our knowledge, the first experimental demonstration of a new imaging system for in situ measurement of the two-dimensional (2D) distribution of the surface temperature of microscopic specimens. The main component of the system is an imaging tandem acousto-optical tunable filter (TAOTF) synchronized with a video camera. A set of TAOTF spectroscopic images (up to a few hundreds) is taken by the TAOTF imaging system to fit the measured spectral curves in each pixel to the Planck radiation function and determine the temperature and emissivity of the sample using the gray body approximation. It is experimentally shown that this technique provides aberration-free spectral imaging suitable for precise multispectral imaging radiometry (MIR).

Acousto-optical tunable filter for combined wideband, spectral, and optical coherence microscopy.

A multimodal technique for inspection of microscopic objects by means of wideband optical microscopy, spectral microscopy, and optical coherence microscopy is described, implemented, and tested. The key feature is the spectral selection of light in the output arm of an interferometer with use of the specialized imaging acousto-optical tunable filter. In this filter, two interfering optical beams are diffracted via the same ultrasound wave without destruction of interference image structure. The basic requirements for the acousto-optical tunable filter are defined, and mathematical formulas for calculation of its parameters are derived. Theoretical estimation of the achievable accuracy of the 3D image reconstruction is presented and experimental proofs are given. It is demonstrated that spectral imaging can also be accompanied by measurement of the quantitative reflectance spectra. Examples of inspection of optically transparent and nontransparent samples demonstrate the applicability of the technique.

Where in the tissues of Danio rerio is more H2O2 produced during acute hypoxia?

The lack of oxygen (O2) causes changes in the cell functioning. Modeling hypoxic conditions in vitro is challenging given that different cell types exhibit different sensitivities to tissue O2 levels. We present an effective in vivo platform for assessing various tissue and organ parameters in Danio rerio larvae under acute hypoxic conditions. Our system allows simultaneous positioning of multiple individuals within a chamber where O2 level in the water can be precisely and promptly regulated, all while conducting microscopy. We applied this approach in combination with a genetically encoded pH-biosensor SypHer3s and a highly H2O2-sensitive Hyper7 biosensor. Hypoxia causes H2O2 production in areas of brain, heart and skeletal muscles, exclusively in the mitochondrial matrix; it is noteworthy that H2O2 does not penetrate into the cytosol and is neutralized in the matrix upon reoxygenation. Hypoxia causes pronounced tissue acidosis, expressed by a decrease in pH by 0.4-0.6 units everywhere. Using imaging photoplethysmography, we measured in D.rerio fry real-time heart rate decrease under conditions of hypoxia and subsequent reoxygenation. Our observations in this experimental system lead to the hypothesis that mitochondria are the only source of H2O2 in cells of D.rerio under hypoxia.

Evaluation of Acoustic Waves in Acousto-Optical Devices by Ultrasonic Imaging.

The structure of the acoustic field defines the key parameters of acousto-optical (AO) devices. To confirm their compliance with the expected values in the presence of multiple real factors, AO crystalline cells require accurate experimental investigation of the acoustic field after being totally assembled. For this purpose, we propose to detect and quantify all the acoustic waves propagating in AO cells using an impulse acoustic microscopy technique. To validate this approach, we have analyzed both theoretically and experimentally the modes, amplitudes, propagation trajectories, and other features of the ultrasonic waves generated inside an AO modulator made of fused quartz. Good correspondence between theoretical and experimental data confirms the effectiveness of the proposed technique.

Exoscope-based videocapillaroscopy system for in vivo skin microcirculation imaging of various body areas.

The capillary system immediately responds to many pathologies and environmental conditions. Accurate monitoring of its functioning often enables early detection of various diseases related to disorders in skin microcirculation. To expand the scope of capillaroscopy application, it is reasonable to visualize and assess blood microcirculation exactly in the areas of inflamed skin. Body vibrations, breathing, non-flat skin surface and other factors hamper the application of conventional capillaroscopes outside the nailfold area. In this paper, we propose an exoscope-based optical system for high-quality non-invasive computational imaging of capillary network in various areas of the body. Accurate image matching and tracking temporal intensity variations allow detecting the presence of blood pulsations, precise mapping of capillaries and photoplethysmogram acquisition. We have demonstrated the efficiency of the proposed approach experimentally by in vivo mapping and analysis of microvessels in wrist, forearm, upper-arm, breast and hip areas. We believe that the developed system will increase the diagnostic value of video capillaroscopy in clinical practice.

Imaging photoplethysmography and videocapillaroscopy enable noninvasive study of zebrafish cardiovascular system functioning.

We report on the noninvasive method for in vivo study of fish's cardiovascular system, that is, the heart and the structure of vessels that carry blood throughout the body. The proposed approach is based on combined photoplethysmographic and videocapillaroscopic microscopic imaging and enables noncontact two-dimensional mapping of blood volume changes. We demonstrate that the obtained data allows precise measurements of heartbeat, blood flow velocity and other important parameters (see Videos S1 and S2). To validate the developed image processing technique, we have carried out multiple experiments on zebrafish-a well-proven informative model organism widely used to understand cardiac development. The proposed approach may be effective for the study of cardiovascular system formation and functioning as well as the impact of various influencing factors on them.

Control of Acousto-Optic Mode Locker by Means of Electronic Matching Circuit.

An acousto-optic mode locker (AOML) operating in a standing wave mode has a fixed working frequency, defined by the length of the crystal, which makes the device prone to temperature variations. In this study, we examine the effect of the electrical matching circuit parameters on the acoustic resonances and AO diffraction in the AOML both theoretically and experimentally. Acoustical and electrical models of the AOML are introduced. We outline the ways of utilizing this effect for compensation of ill thermal influence. Our analysis allows us to broaden the temperature and driving power domains of an AOML's reliable operation without a heat sink or a complicated temperature stabilization system.

Blood Vessel Imaging at Pre-Larval Stages of Zebrafish Embryonic Development.

The zebrafish (Danio rerio) is an increasingly popular animal model biological system. In cardiovascular research, it has been used to model specific cardiac phenomena as well as to identify novel therapies for human cardiovascular disease. While the zebrafish cardiovascular system functioning is well examined at larval stages, the mechanisms by which vessel activity is initiated remain a subject of intense investigation. In this research, we report on an in vivo stain-free blood vessel imaging technique at pre-larval stages of zebrafish embryonic development. We have developed the algorithm for the enhancement, alignment and spatiotemporal analysis of bright-field microscopy images of zebrafish embryos. It enables the detection, mapping and quantitative characterization of cardiac activity across the whole specimen. To validate the proposed approach, we have analyzed multiple data cubes, calculated vessel images and evaluated blood flow velocity and heart rate dynamics in the absence of any anesthesia. This non-invasive technique may shed light on the mechanism of vessel activity initiation and stabilization as well as the cardiovascular system's susceptibility to environmental stressors at early developmental stages.