Design of a refractive-metasurface hybrid annular aperture folded optical system.

Folded lenses offer advantages in terms of lightness and thinness, but they have limitations when it comes to correcting aberrations. In this paper, we propose a novel approach to address this issue by incorporating metasurfaces in the design of folded optical systems. Specifically, a folded refractive-metasurface hybrid annular aperture folded lens (AFL) is introduced. The structural characteristics of the AFL imaging system are analyzed to investigate the blocking ratio, thickness, and light collection capability of the ring aperture system. Additionally, a hybrid optical integration design using Zemax software is proposed for the metasurfaces. A quadruple-folded AFL working in the mid-infrared waveband is then designed. The superstructure surface is analyzed, and its processability is discussed. The results demonstrate that the reflective-metasurface hybrid AFL significantly improves the imaging quality of this type of optical system while meeting the required design accuracy.

Digital-optical co-design enables athermalization of hybrid optical systems.

We proposed a digital-optical co-design that can effectively improve the image quality of refractive-diffractive hybrid imaging systems over a wide ambient temperature range. Diffraction theory was used to establish the degradation model and blind deconvolution image recovery algorithm was used to perform recovery for simulated images. The peak signal-to-noise ratio (PSNR) and structural similarity (SSIM) were used to evaluate the algorithm performance. A cooled athermalization dual-band infrared optical system with a double-layer diffractive optical element (DLDOE) was designed, and the results show an overall improvement for both PSNR and SSIM overall the whole ambient temperature range. This demonstrates the effectiveness of the proposed method for the image quality improvement of hybrid optical systems.

Identification of Novel Biomarkers for Abdominal Aortic Aneurysm Promoted by Obstructive Sleep Apnea.

BACKGROUND: We sought to find new biomarkers for abdominal aortic aneurysms (AAA) caused by chronic intermittent hypoxia (CIH). METHODS: The AAA mice model was created using Ang II. The mice were divided into normoxic and CIH groups. The structure of AAA was observed using abdominal ultrasonography, Elastica van Gieson (EVG), and hematoxylin and eosin (HE) staining. The expression of ɑ-SMA was investigated using immunohistochemistry. The novel biomarkers were screened using bioinformatics analysis. Quantitative real-time polymerase chain reaction (qRT-PCR) was employed to verify the expression of novel genes in both normal oxygen and CIH. RESULTS: CIH appears to cause greater aortic dilation, higher AAA incidence, lower survival rate, thicker vessel wall, and more brittle elastic lamellae when compared to controls. The immunohistochemistry results showed that the expression of ɑ-SMA in the CIH group was reduced significantly. Four novel genes, including Homer2, Robo2, Ehf, and Asic1, were found to be differentially expressed between normal oxygen and CIH using qRT-PCR, indicating the same trend as bioinformatics analysis. CONCLUSIONS: We discovered that CIH could hasten the occurrence and progression of AAA. Four genes (Homer2, Robo2, Ehf, and Asic1) may be novel biomarkers for AAA, which could aid in the search for new therapies for patients with AAA caused by CIH.

Activity engagement and cognitive function among chinese older adults: moderating roles of gender and age.

BACKGROUND: Many studies have found that engaging in activities, including physical exercise, social interaction, and cognitive training, is beneficial for preventing cognitive decline among older adults; however, the demographic differences in the association between activity engagement and cognitive functions remain understudied. This study investigates: (a) the influence of activity engagement on cognitive functions among Chinese older adults, and (b) the moderating roles of age and gender in these associations . METHODS: The data were derived from the China Health and Retirement Longitudinal Study in 2018, which included 9803 participants aged 60 or older. A multiple regression model was used to test the study hypotheses. RESULTS: Engaging in physical activity (b = 1.578, p < .001), social interaction (b = 1.199, p < .001), and cognitive activity (b = 1.468, p < .001) was positively associated with cognitive functions, whereas the effect of volunteer activities on cognitive functions was not significant (b = -.167, p = .390). Light- and moderate-intensity activities were beneficial for cognition (light: b = .847, p < .001; moderate: b = 1.189, p < .001), but vigorous-intensity activity was negatively related to cognition (b = -.767, p < .001). In addition, women and participants with advanced age appeared to benefit more from cognitive activities than their male and younger counterparts, respectively (gender: b = 1.217, p = .002; age: b = .086, p = .004). The adverse effects of vigorous-intensity activities (including agricultural work) on cognitive health were stronger for women and younger participants (gender: b = -1.472, p < .001; age: b = .115, p < .001). The protective effects of moderate-intensity activities on people's cognition increased with increasing age (b = .055, p = .012). CONCLUSIONS: The findings indicate that participating in physical, social, and cognitive activities can help older adults to maintain cognitive health. They suggest that older adults should select activities while considering activity intensity and their individual characteristics.

Perceptions of neighborhood environment and loneliness among older Chinese adults: the mediator role of cognitive and structural social capital.

OBJECTIVE: This study aimed to examine the mediating role of social capital in the association between perceived physical neighborhood environment and loneliness among older adults in urban China. METHOD: Data were derived from face-to-face interviews with people (n = 472) aged 60 or older in urban communities of Shanghai, China, in 2020. Loneliness was measured by the 6-item De Jong Gierveld Scale. A multiple mediator model was adopted to examine the research hypotheses. RESULTS: Cognitive social capital played a full mediation role in the association between perceived community health care (ß = -.061, p < .05), safety (ß = -.168, p < .001), recreational services (ß = -.058, p < .05), and loneliness. Structural social capital was associated with loneliness (ß = -.102, p < .05), whereas its mediator effect was not significant. Perceived transportation convenience had neither a direct nor an indirect effect on loneliness among older adults. CONCLUSION: Findings indicate that older adults' perceptions of healthcare services, safety, and recreational resources could help alleviate their loneliness via fostering cognitive social capital. Interventions improving older adults' perceptions of community health care, safety, and recreation, and their social capital level could help reduce the prevalence of loneliness.

Breast-conserving surgery is an appropriate procedure for centrally located breast cancer: a population-based retrospective cohort study.

BACKGROUND: The evidence of breast-conserving therapy (BCT) applied in centrally located breast cancer (CLBC) is absent. This study aims to investigate the long-term survival of breast-conserving therapy (BCT) in centrally located breast cancer (CLBC) compared with mastectomy in CLBC and BCT in non-CLBC. METHODS: Two hundred ten thousand four hundred nine women with unilateral T1-2 breast cancer undergoing BCT or mastectomy were identified from the Surveillance, Epidemiology, and End Results database. Kaplan-Meier survival curves were assessed via log-rank test. Propensity score matching (PSM) was used to balance baseline features, and the multivariable Cox model was used to estimate the adjusted hazard ratio [HR] and its 95% confidence interval [CI] for breast cancer-specific survival (BCSS) and overall survival (OS). RESULTS: With a median follow-up of 91 months, the BCSS and OS rates in patients who received BCT were greater than those patients treated with mastectomy in the entire CLBC set. Multivariable Cox analyses showed that CLBC patients who received BCT had better BCSS (HR = 0.67, 95%CI: 0.55-0.80, p < 0.001) and OS (HR = 0.78, 95%CI: 0.68-0.90, p = 0.001) than patients who received a mastectomy, but there were no significant differences of BCSS (HR = 0.65, 95%CI: 0.47-0.90, p = 0.009) and OS (HR = 0.82, 95%CI: 0.65-1.04, p = 0.110) after PSM. In patients treated with BCT, CLBC patients had a similar BCSS (HR = 0.99, 95%CI: 0.87-1.12, p = 0.850) but a worse OS (HR = 1.09, 95%CI: 1.01-1.18, p = 0.040) compared to that of the non-CLBC patient, but there was no significant difference both BCSS (HR = 1.05, 95%CI: 0.88-1.24, p = 0.614) and OS (HR = 1.08, 95%CI: 0.97-1.20, p = 0.168) after PSM. CONCLUSION: Our findings revealed that BCT should be an acceptable and preferable alternative to mastectomy for well-selected patients with CLBC.

Activity Patterns and Loneliness in Later Life: The Mediating Effects of Social Support and Attitudes Toward Aging.

Studies have documented the inconsistent influences of activity participation on health. However, the underlying mechanisms connecting activity patterns to loneliness are relatively understudied. Using data on 8,545 respondents aged 60 or older from the 2014 China Longitudinal Aging Social Survey, we used latent class analysis to identify the patterns of participating in eight activities and path analysis to examine the mediating effects of formal support, informal support, and aging attitudes in the relationship of activity patterns and loneliness. Three meaningful patterns of activity (high activity, low activity, and working caregivers) were identified. Working caregivers and low activity groups, compared to high activity, showed higher levels of loneliness in later life. Informal support, rather than formal support, and attitudes toward aging mediated the relationship between activity patterns and loneliness in later life. This study highlights the importance of fuller engagement in diverse political, economic, and community activities to prevent loneliness. Additionally, intervention programs that facilitate joint support from family, community, and government and shape scientific understanding of aging are critical for alleviating loneliness in later life.

Computational Imaging in Dual-Band Infrared Hybrid Optical System with Wide Temperature Range.

The special dispersion and temperature characteristics of diffractive optical element (DOE) make them widely used in optical systems that require both athermalization and achromatic aberrations designs. The multi-layer DOE (MLDOE) can improve the diffraction efficiency of the overall broad waveband, but its diffraction efficiency decreases with changes in ambient temperature. When the ambient temperature changes, the micro-structure heights of MLDOE and the refractive index of the substrate materials change, ultimately affecting its diffraction efficiency, and, further, the optical transform function (OTF). In this paper, the influence of ambient temperature on the diffraction efficiency of MLDOE in a dual-infrared waveband is proposed and discussed, the diffraction efficiency of MLDOE caused by ambient temperature is derived, and a computational imaging method that combines optical design and image restoration is proposed. Finally, a dual-infrared waveband infrared optical system with athermalization and achromatic aberrations corrected based on computational imaging method is designed. Results show that this method can effectively reduce the diffraction efficiency of MLDOE by ambient temperature and improve the imaging quality of hybrid optical systems.

Design and analysis of a hybrid optical system containing a multilayer diffractive optical element with improved diffraction efficiency.

We present a compressive image quality evaluation for a hybrid optical system containing a multilayer diffractive optical element (MLDOE). We take effects from both incident angle and waveband into consideration to improve the diffraction efficiency of the MLDOE. With this MLDOE design, it can ensure accurate image quality evaluation for hybrid optical systems. The results show that this design enables a diffraction-efficiency-improved MLDOE, and further ensures accurate image quality of the modulation transfer function and better optical system structure of the hybrid surveillance lens. The results are of great significance for the optimal design of hybrid imaging optical systems.

Ezrin, a Membrane Cytoskeleton Cross-Linker Protein, as a Marker of Epithelial Damage in Asthma.

RATIONALE: Bronchial epithelial cell damage occurs in patients with bronchial asthma. Ezrin, a membrane-cytoskeleton protein, maintains cellular morphology and intercellular adhesion and protects the barrier function of epithelial cells. OBJECTIVES: To study the role of ezrin in bronchial epithelial cells injury and correlate its expression with asthma severity. METHODS: Levels of ezrin were measured in exhaled breath condensate (EBC) and serum in patients with asthma and BAL fluid (BALF) from a mouse model of asthma by ELISA. The regulation of IL-13 on ezrin protein levels was studied in primary bronchial epithelial cells. Ezrin knockdown using shRNA was studied in human bronchial epithelial 16HBE cells. MEASUREMENTS AND MAIN RESULTS: Ezrin levels were decreased in asthmatic EBC (92.7 ± 34.99 vs. 150.5 ± 10.22 pg/ml, P < 0.0001) and serum (700.7 ± 55.59 vs. 279.2 ± 25.83 pg/ml, P < 0.0001) compared with normal subjects. Levels were much lower in uncontrolled (P < 0.001) and partly controlled patients (P < 0.01) compared with well-controlled subjects. EBC and serum ezrin levels correlated with lung function in patients with asthma and serum ezrin levels were negatively correlated with serum IL-13 and periostin. IL-13-induced downregulation of ezrin expression in primary bronchial epithelial cells was significantly attenuated by the Janus tyrosine kinase 2 inhibitor, TG101348. Ezrin knockdown changed 16HBE cell morphology, enlarged intercellular spaces, and increased their permeability. Ezrin expression was decreased in the lung tissue and BALF of "asthmatic" mice and negatively correlated with BALF IL-13 level. CONCLUSIONS: Ezrin downregulation is associated with IL-13-induced epithelial damage and might be a potential biomarker of asthma control.

Integral diffraction efficiency model for multilayer diffractive optical elements with wide angles of incidence in case of polychromatic light.

Oblique incidence is the normal working mode for diffractive optical elements (DOEs). The diffraction efficiency is very sensitive to the angle of incidence for multilayer diffractive optical elements (MLDOEs). Therefore, the design and diffraction efficiency analysis of MLDOEs with wide angles of incidence is of universal significance and practice. We propose an integral diffraction efficiency model for MLDOEs with wide angles of incidence in case of polychromatic light and then describe this corresponding optimal design in detail. It is shown that high diffraction efficiency can be realized by the surface micro-structure heights optimization, ensuring high modulation transfer function (MTF) for MLDOEs with wide angles of incidence in hybrid optical systems. On this basis, we present the optimal design process and simulation of an MLDOE working in visible waveband with optical plastic materials combination PMMA and POLYCARB as the two-layer substrates. The result shows that with this optimal design, we can achieve the maximum diffraction efficiency and minimum micro-structure heights, which makes the MLDOE design exactly over the entire waveband and wide angles of incidence especially for zoom hybrid optical system.

Tolerance analysis on decenter error of multilayer diffractive optical elements based on polychromatic integral diffraction efficiency.

The decenter error can determine the polychromatic integral diffraction efficiency (PIDE) over a broad wave band of hybrid optical systems and further the imaging quality. The mathematical model of the relationship between the decenter error and the PIDE of multilayer diffractive optical elements (MLDOEs) is presented, and the expressions are derived based on phase delay of diffractive optical elements for both normal and oblique incident situations. Finally, the sensitivity of PIDE to the decenter error for MLDOEs used in a long-infrared wave band with a material combination of ZNS-ZNSE is analyzed, and then the decenter error tolerance is proposed to ensure high PIDE over a broad wave band as well as diffraction efficiency at the designed wavelengths. The analysis methods and results could be useful for guiding the decenter error of MLDOEs in hybrid optical systems for optical engineers.

Puerarin prevents diabetic cardiomyopathy in vivo and in vitro by inhibition of inflammation.

Diabetic cardiomyopathy (DCM) is one of the chief diabetes mellitus complications. Inflammation factors may be one reason for the damage from DM. The purpose of this research is to study the potential protective effects of puerarin on DM and the possible mechanisms of action related to NF-κB signal pathway. Following administration of puerarin to the disease model rat, several changes were observed including the changes of serum biochemical index, improved diastolic dysfunction, and enhanced endogenous antioxidant enzymes activities, further NF-κB signaling activation. Puerarin showed cardio-protective effects on DCM by inhibiting inflammation, and it might be a potential candidate for the treatment of DCM.

[Research advances in the biomarkers of brain damage in preterm infants].

While the survival rate of preterm infants has continually increased with the development of perinatal and neonatal monitoring techniques, the incidence of brain injury in preterm infants has been increasing, resulting in varying degrees of cognitive impairment and movement disorders. Measuring the biomarkers of brain damage is an important means to diagnose brain injury. The biomarkers can be divided into neuroglial damage markers, neuronal damage markers and other markers according to the features of injured cells. The biomarkers widely used in clinical practice include S100B protein, myelin basic protein and neuron-specific enolase. Recent studies have newly discovered a collection of markers that can suggest potential brain injury in preterm infants, such as glial fibrillary acidic protein, neurofilament light chain protein, α-II spectrin breakdown products, chemokines, melatonin and urinary metabolomics. These biomarkers can contribute to the early diagnosis and treatment of preterm brain injury, essential for improving neural development and prognosis. This article reviews the latest research advances in the biomarkers of preterm brain injury, in order to provide evidence for the early diagnosis and treatment of this condition.

Effect of the surface roughness on the efficiency of diffractive optical elements.

The proposed study aims to investigate the effect of surface roughness on the efficiency of diffractive optical elements (DOEs) used in imaging optical systems. A mathematical model describing the influence of surface roughness on diffraction efficiency and polychromatic integral diffraction efficiency (PIDE) is presented, and its effects are analyzed and simulated. Examples of single-layer DOEs containing PMMA substrate and multi-layer DOEs composed of a combination of PMMA and polycarbonate substrates for each layer used in the visible region are analyzed. The results can be used to analyze the effects of surface roughness on the diffraction efficiency and PIDE of DOEs.

[HPLC fingerprints of pig, cattle and sheep biles].

To establish the fingerprints of biles of pig, cattle and sheep, HPLC was used with Acclaim™ RSLC 120 C18 column (3.0 mm×100 mm, 2.2 µm, 120 Å), the column temperature 35 °C, acetonitrile-1% perchloric acid as mobile phase, gradient elution, 0.5 mL·min⁻¹ flow rate, and detection wavelength at 200 nm. The fingerprint was generated by using Similarity Evaluation Software of Chromatographic Fingerprint of Chinese Medicine (2004A Edition). The fingerprint peaks were identified by reference substances and verified by ELSD and LC-MS/MS. Then, the biles of pig, cattle and sheep were detected to contain 14, 9 and 8 common fingerprint peaks respectively, and the similarity was greater than 0.92. To analyze each technical parameter, GHDCA in pig bile and TCA in cattle and sheep bile were selected as reference peak. The precision, repeatability and stability all meet the requirements of fingerprint establishment. The RSD of the relative retention time of the fingerprint peaks was less than 1.5%, and the RSD of the relative peak area was less than 5%. The fingerprint peaks in pig bile were THDCA, TCDCA, GHDCA and GCDCA, and TCA, TCDCA, GCA, GCDCA and GDCA in cattle and sheep bile. The main components of pig, cattle and sheep bile were conjugated bile acids, but there were significant differences in bile acids between pig bile and cattle, sheep biles. The HPLC method established in this paper is simple, rapid and reproducible, and could be applied to the identification and quality control of biles.

Optimal design method on diffractive optical elements with antireflection coatings.

The effect of antireflection coatings on diffraction efficiency of diffractive optical elements (DOEs) was studied and the mathematical model of diffraction efficiency affected by antireflection coatings for DOEs is presented. We found antireflection coatings can cause a significant reduction on diffraction efficiency at the designed, or the central wavelength. In order to solve this problem, we proposed a method to keep 100% diffraction efficiency at the designed wavelength by ensuring the 2π phase induced by DOEs and the antireflection coatings. Diffraction efficiency affected by antireflection coatings for DOEs with consideration of antireflection coatings are simulated. Analysis results can be utilized for refractive-diffractive hybrid imaging optical system optimal design and image quality evaluation.

Substrate material selection method for multilayer diffractive optics in a wide environmental temperature range.

We present a substrate material selection method for multilayer diffractive optical elements (MLDOEs) to obtain high polychromatic integral diffraction efficiency (PIDE) in a wide environmental temperature range. The extended expressions of the surface relief heights for the MLDOEs are deduced with consideration of the influence of the environmental temperature. The PIDE difference Δη¯(λ) and PIDE change factor F are introduced to select a reasonable substrate material combination. A smaller value of Δη¯(λ) or F indicates a smaller decrease of the PIDE in a wide temperature range, and the corresponding substrate material combination is better. According to the deduced relation, double-layer and three-layer DOEs with different combinations are discussed. The results show that IRG26 and zinc sulfide is the best substrate material combination in the infrared waveband for double-layer DOEs, and polycarbonate is more reasonable than polymethyl methacrylate as the middle filling optical material for three-layer DOEs when the two substrate materials are the same.

[Pro-inflammatory mechanism of lectin from Pinellia pedatisecta on macrophage].

To investigate the mechanism of lectin from Pinellia pedatisecta(PPL) on macrophage-induced inflammation and its association with inflammatory corpuscles NLRP3. Lectin from P. pedatisecta was isolated and purified by gel chromatography, and its purity was analyzed by using SDS-PAGE gel electrophoresis. ELISA was used to investigate the effect of PPL on inflammatory cytokines released by macrophages, with IL-1ß as indicators;and fluorescence probe DCFH-DA fluorometer was used to determine changes in active oxygen ROS of macrophages after application of lectin from P. pedatisecta.RAW264.7 cells were pre-treated with ROS inhibitor N-acetylcysteine (NAC) to investigate the effect on ROS and the release of inflammatory factor IL-1ß from macrophages to research the relationship between them. The protein levels of NLRP3, Caspase-1 p20, ASC and TXNIP were determined by Western blot.The results showed that isolated and purified PPL could reach electrophoretic purity; PPL stimulated macrophages and induced the excessive release of ROS, leading to strong oxidative stress reaction, and the levels of intracellular inflammatory factorsIL-1ß were significantly increased. NAC could inhibit PPL-induced ROS excessive production and significantly reduce the release of IL-1ß. In addition, PPL could induce the increase in protein expression levels of Caspase-1 p20, NLRP3 and ASC, and significantly reduce TXNIP expression. The results showed that PPL could cause a strong oxidative stress response by stimulating macrophages, activate inflammatory corpuscles NLRP3, and result in large amount of IL-1ß release. That is, PPL could lead to inflammatory cascade reaction by promoting the maturation and secretion of IL-1ß through ROS-TXNIP-NLRP3-IL-1ß signaling pathway.

Clinically mild encephalitis/encephalopathy with a reversible splenial lesion associated with Mycoplasma pneumoniae infection.

BACKGROUND: Clinically mild encephalitis/encephalopathy with a reversible splenial lesion (MERS) is a clinico-radiological syndrome characterized by transient mild symptoms of encephalopathy and a reversible lesion in the splenium of the corpus callosum on magnetic resonance imaging (MRI). It is often triggered by infection. The common pathogens of MERS are viruses, especially influenza virus. However, Mycoplasma pneumoniae (M.pneumoniae) are relatively rare pathogens for MERS. CASE PRESENTATION: Here we report two paediatric cases of M.pneumoniae infection-induced MERS. The diagnosis of M.pneumoniae infection was established based on polymerase chain reaction (PCR) and specific serum antibodies (IgM). Both of the two patients presented with mild encephalopathy manifestations and recovered completely within a few days. The initial MRI showed a lesion in the central portion of the splenium of the corpus callosum, which completely resolved on the seventh and eighth day after admission for case 1 and case 2. Lumbar puncture was performed in both patients, which revealed no pleocytosis. In case 1, the patient had hyponatremia, peripheral facial nerve paralysis, and rash. To the best of our knowledge, it is the first MERS case associated with peripheral nerve damage. In case 2, interleukin-6(IL-6) was moderately increased in the cerebrospinal fluid (CSF). It suggested that IL-6 may play a role in the pathogenesis of M.pneumoniae-induced MERS. CONCLUSION: Our study enriches the available information on the pathogens of MERS and provides valuable data for better understanding of this syndrome.