RESUMEN
Twenty Campylobacter jejuni and 16 Campylobacter coli strains isolated from humans and food/animals, including 17 isolates resistant to erythromycin, were analyzed. A combined mismatch amplification mutation assay-PCR technique was developed to detect the mutations A 2074 C and A 2075 G in the 23S rRNA gene associated with erythromycin resistance. All high-level erythromycin-resistant strains examined by DNA sequencing carried the transition mutation A 2075 G, whereas no isolate carried the A 2074 C mutation. No mutations were found among the susceptible and low-level erythromycin-resistant strains.
Asunto(s)
Campylobacter coli/efectos de los fármacos , Campylobacter jejuni/efectos de los fármacos , Farmacorresistencia Bacteriana/genética , Eritromicina/farmacología , Mutación Puntual , Reacción en Cadena de la Polimerasa/métodos , Animales , Infecciones por Campylobacter/microbiología , Campylobacter coli/genética , Campylobacter jejuni/genética , Pollos , Humanos , Productos Avícolas/microbiología , ARN Bacteriano/genética , ARN Ribosómico 23S/genética , PorcinosRESUMEN
A multiplex PCR was developed for simultaneous detection of the cytolethal distending toxin (cdt) genes of Campylobacter jejuni. Three primer pairs targeting each one of the cdtA, cdtB and cdtC genes were designed and combined in the same PCR reaction. The assay was evaluated with 100 C. jejuni strains recovered from humans and animals and it was found to be rapid and specific. Two isolates presented several deletions affecting both cdtA and cdtB genes. High prevalence (98%) of the three cdt genes was found among isolates of different geographic origins.
Asunto(s)
Toxinas Bacterianas/genética , Campylobacter jejuni/genética , Animales , Toxinas Bacterianas/química , Secuencia de Bases , Infecciones por Campylobacter/microbiología , Campylobacter jejuni/aislamiento & purificación , ADN Bacteriano/análisis , ADN Bacteriano/genética , Genes Bacterianos , Humanos , Datos de Secuencia Molecular , Reacción en Cadena de la Polimerasa/métodos , Análisis de Secuencia de ADNRESUMEN
A fragment of the gyrA gene was sequenced from 34 isolates of Campylobacter coli, including 23 isolates resistant to ciprofloxacin. All ciprofloxacin-resistant isolates examined by DNA sequencing carried a point mutation at position Thr-86 on the gyrA gene product, involving the replacement of Thr-86 by Ile. A combined PCR-restriction fragment length polymorphism technique using RsaI was developed to detect this mutation.