RESUMEN
We determined the effect of conjugated equine estrogen plus medroxyprogesterone acetate on calcium content of aortic atherosclerotic lesions in oophorectomized adult New Zealand rabbits submitted to a cholesterol rich diet. Five groups of 10 animals each were studied: G1 = control, G2 = cholesterol diet only, G3 = diet plus conjugated equine estrogen (0.625 mg/day); G4 and G5 = diet, conjugated equine estrogen (0.625 mg/day) plus medroxyprogesterone acetate (5 and 10 mg/day, respectively). Mean weight varied from 2.7 +/- 0.27 to 3.1 +/- 0.20 kg (P = 0.38) between groups at the beginning and 3.1 +/- 0.27 to 3.5 +/- 0.20 kg (P = 0.35) at the end of the experiment. Cholesterol and triglyceride levels were determined at the time of oophorectomy, 21 days after surgery (time 0), and at the end of follow-up of 90 days. The planimetric method was used to measure plaque and caryometric method for histopathologic examination of the aorta. Calcium content was determined by the method of von Kossa. A similar increase in cholesterol occurred in all treated groups without differences between them at the end of the study. Groups G4 and G5 had smaller areas of atherosclerotic lesions (2.33 +/- 2.8 and 2.45 +/- 2.1 cm(2), respectively) than the groups receiving no progestogens (G2: 5.6 +/- 4 and G3: 4.6 +/- 2.8 cm(2); P = 0.02). The relation between lesion area and total aorta area was smaller in groups treated with combined drugs compared to the groups receiving no progesterone (G4: 14.9 +/- 13 and G5: 14.2 +/- 13.4 vs G2: 35.8 +/- 26 and G3: 25 +/- 8 cm(2), respectively; P = 0.017). Oral conjugated equine estrogen (0.625 mg/day) plus medroxyprogesterone acetate (5 or 10 mg/day) provoked a greater reduction in atherosclerotic plaque area and calcium content in treated groups, suggesting a dose-dependent effect.
Asunto(s)
Aorta/química , Aterosclerosis/metabolismo , Calcio/análisis , Estrógenos Conjugados (USP)/farmacología , Acetato de Medroxiprogesterona/farmacología , Animales , Aorta/efectos de los fármacos , Aterosclerosis/etiología , Calcio/metabolismo , Colesterol/análisis , Dieta Aterogénica , Relación Dosis-Respuesta a Droga , Femenino , Ovariectomía , Conejos , Distribución Aleatoria , Factores de Tiempo , Triglicéridos/análisisAsunto(s)
Aterosclerosis/terapia , Colesterol/sangre , Dislipidemias/terapia , Adolescente , Adulto , Anciano , Aterosclerosis/prevención & control , Biomarcadores/sangre , Enfermedades Cardiovasculares/etiología , Niño , Ésteres del Colesterol/sangre , Dislipidemias/sangre , Dislipidemias/prevención & control , Ácidos Grasos/metabolismo , Femenino , Ácidos Fíbricos/metabolismo , Humanos , Hipercolesterolemia/sangre , Hipertrigliceridemia/sangre , Esperanza de Vida , Lipoproteínas/metabolismo , Masculino , Persona de Mediana Edad , Factores de Riesgo , Triglicéridos/sangreAsunto(s)
Adolescente , Adulto , Anciano , Niño , Femenino , Humanos , Masculino , Persona de Mediana Edad , Aterosclerosis/terapia , Colesterol/sangre , Dislipidemias/terapia , Aterosclerosis/prevención & control , Biomarcadores/sangre , Enfermedades Cardiovasculares/etiología , Ésteres del Colesterol/sangre , Dislipidemias/sangre , Dislipidemias/prevención & control , Ácidos Grasos/metabolismo , Ácidos Fíbricos/metabolismo , Hipercolesterolemia/sangre , Hipertrigliceridemia/sangre , Esperanza de Vida , Lipoproteínas/metabolismo , Factores de Riesgo , Triglicéridos/sangreRESUMEN
We determined the effect of conjugated equine estrogen plus medroxyprogesterone acetate on calcium content of aortic atherosclerotic lesions in oophorectomized adult New Zealand rabbits submitted to a cholesterol rich diet. Five groups of 10 animals each were studied: G1 = control, G2 = cholesterol diet only, G3 = diet plus conjugated equine estrogen (0.625 mg/day); G4 and G5 = diet, conjugated equine estrogen (0.625 mg/day) plus medroxyprogesterone acetate (5 and 10 mg/day, respectively). Mean weight varied from 2.7 ± 0.27 to 3.1 ± 0.20 kg (P = 0.38) between groups at the beginning and 3.1 ± 0.27 to 3.5 ± 0.20 kg (P = 0.35) at the end of the experiment. Cholesterol and triglyceride levels were determined at the time of oophorectomy, 21 days after surgery (time 0), and at the end of follow-up of 90 days. The planimetric method was used to measure plaque and caryometric method for histopathologic examination of the aorta. Calcium content was determined by the method of von Kossa. A similar increase in cholesterol occurred in all treated groups without differences between them at the end of the study. Groups G4 and G5 had smaller areas of atherosclerotic lesions (2.33 ± 2.8 and 2.45 ± 2.1 cm², respectively) than the groups receiving no progestogens (G2: 5.6 ± 4 and G3: 4.6 ± 2.8 cm²; P = 0.02). The relation between lesion area and total aorta area was smaller in groups treated with combined drugs compared to the groups receiving no progesterone (G4: 14.9 ± 13 and G5: 14.2 ± 13.4 vs G2: 35.8 ± 26 and G3: 25 ± 8 cm², respectively; P = 0.017). Oral conjugated equine estrogen (0.625 mg/day) plus medroxyprogesterone acetate (5 or 10 mg/day) provoked a greater reduction in atherosclerotic plaque area and calcium content in treated groups, suggesting a dose-dependent effect.
Asunto(s)
Conejos , Animales , Femenino , Aorta/química , Arteriosclerosis/metabolismo , Calcio/análisis , Dieta Aterogénica , Estrógenos Conjugados (USP)/farmacología , /farmacología , Aorta/efectos de los fármacos , Calcio/metabolismo , Relación Dosis-Respuesta a Droga , Ovariectomía , Factores de TiempoAsunto(s)
Calcinosis/diagnóstico por imagen , Enfermedad de la Arteria Coronaria/diagnóstico por imagen , Hiperlipoproteinemia Tipo II/complicaciones , Adulto , Distribución por Edad , Anciano , Anciano de 80 o más Años , Calcinosis/complicaciones , Enfermedad de la Arteria Coronaria/complicaciones , Heterocigoto , Humanos , Persona de Mediana Edad , Tomografía Computarizada por Rayos XRESUMEN
Objetivo - avaliar os efeitos do etofibrato sobre variáveis lipídicas, fibrinogênio e agregaçäo plaquetária. Métodos - foram selecionados 21 portadores de hiperlipidemia primária, associada a fatores de risco para doença coronária, após introduçäo de dieta (AHA fase I) e dias, analisando-se as modificaçöes lipídicas induzidas (colesterol total e fraçöes, triglicérides) e efeitos sobre o fibrinogênio e agregaçäo plaquetária. Resultados - foram observadas reduçöes percentuais significantes das variávs: colesterol total (9,50 por cento), LDL-colesterol (-7,88 por cento), triglicérides (19,07 por cento), colesterol total/HDL-colesterol (-11,90 por cento), LDL-colesterol/HDL-colesterol (-19,20 por cento), fibrinogênio (-12, 79 por cento) agregaçäo plaquetária com adrenalina (-24,02 por cento), com ADP 1 ymol (-30,13 por cento), com ADP 3 ymol (-24,51 por cento). Conclusäo - efietos benéficos do etofibrato foram observados näo somente sobre o perfil lipídico mas, também sobre variáveis de trobogenicidae como o fibrinogênio e a agregaçäo plaquetária.