The normality of sperm in an infertile man with ring chromosome 15: a case report.

PURPOSE: The purpose of this report is to analyze the chromosome status and fertilization capability of sperm obtained from an infertile male patient with ring chromosome 15. METHODS: This was a case report at a private in vitro fertilization clinic. A man diagnosed with severe oligozoospermia carrying ring chromosome 15. To evaluate the chromosome status and fertilization capability, sperm from a patient carrying ring chromosome 15 were injected into enucleated mouse oocytes. RESULTS: The karyotypes of motile sperm from a patient carrying ring chromosome 15 were normal, and ring chromosome 15 was not observed in the chromosome spread samples of 1PN. In addition, these motile sperm retained the fertilization capability. However, the fertilization rates decreased (85.2, 76.2, and 64.3%, respectively) along with the decline of the aspect ratio of the sperm head (≥ 1.50, 1.30-1.49, and < 1.30, respectively). CONCLUSIONS: The karyotypes were normal without ring chromosome 15, and motile sperm with a high aspect ratio showed adequate potential for fertilization.

The prevalence and risk factors for delayed union of the superior pubic ramus at one year after curved periacetabular osteotomy: its risk factor and outcome.

PURPOSE: Curved periacetabular osteotomy (CPO) has been developed for the treatment of acetabular dysplasia. While several studies have reported its good clinical results, the complications of CPO include delayed union and nonunion of the superior pubic ramus. The purpose of this study is to investigate the prevalence of delayed union of the pubis one year after CPO, and to determine the risk factors for this complication. METHODS: The study examined 113 hips that underwent CPO between 2008 and 2012. Delayed union was assessed based on the anteroposterior radiography one year after CPO. A superior pubic ramus union group (U group) and a delayed union group (D group) were retrospectively compared regarding patient characteristics, clinical evaluations, and radiographic parameters. RESULTS: Delayed union rate was 16.8%. The D group contained a significantly greater proportion of smokers (p < 0.001). The gap at the pubic osteotomy site on CT coronal images was significantly larger in the D group (p < 0.001), and the cut-off value for the risk of nonunion was larger than 5.1 mm. Multivariate regression analysis indicated that smoking (OR 10.7, 95% CI 2.1-55.4) and a gap at the superior pubic ramus >5.1 mm (OR 16.5, 95% CI 3.7-73.7) were significantly associated with delayed union as independent risk factors. CONCLUSION: The prevalence of delayed union one year after CPO was 16.8%. Smoking and a gap larger than 5.1 mm at the pubic osteotomy site are risk factors for delayed union after CPO.

Incidence of delayed union one year after peri-acetabular osteotomy based on computed tomography.

BACKGROUND: Pubic bone nonunion and delayed union are reported as post-operative complications after peri-acetabular osteotomy (PAO). However, few studies have determined the incidence of delayed union using computed tomography (CT) scans. This study aimed to determine the incidence of delayed union at one year after PAO using X-ray and CT scans. METHODS: We performed a retrospective review of 150 hips in 132 consecutive patients with acetabular dysplasia who underwent PAO between January 2012 and June 2016 and evaluated 107 hips for which pelvic CT scans taken at one year after PAO were available. Clinical evaluations included age at surgery, weight, body mass index (BMI) and history. Radiographic evaluations were to assess pubic, ischial and iliac delayed union at one year post-operatively. RESULTS: Based on X-ray analysis, the incidence of delayed union in the pubic, ischial and iliac bones was 11.2% (12 hips), 5.6% (6 hips) and 0% (0 hips), respectively, and20.6% (22 hips), 8.4% (9 hips) and 0% (0 hips), respectively, based on CT scans. CONCLUSION: The incidence of delayed union of the pubis and ischium at one year after PAO according to CT scans was higher than that based on X-ray imaging. CT scans are useful in patients with some symptoms at the osteotomy site. LEVEL OF EVIDENCE: Level III.

The Utility of False-Profile Radiographs for the Detection of Osteoarthritis Progression in Acetabular Dysplasia.

BACKGROUND: Both the lateral center-edge angle and acetabular roof obliquity on anteroposterior radiographs are well-known prognostic predictors of osteoarthritis progression in patients with acetabular dysplasia. However, few studies have evaluated osteoarthritis progression on false-profile radiographs. In the present study, osteoarthritis progression was evaluated on anteroposterior and false-profile radiographs. METHODS: We retrospectively evaluated 76 patients with acetabular dysplasia with Tönnis grade-0 or 1 osteoarthritis, from a group of 179 patients (209 hips), who had undergone unilateral periacetabular osteotomy from 1995 to 2005. We evaluated the hip joint of the contralateral, untreated side. All patients were followed for ≥10 years. Of the 76 patients, 52 patients with Tönnis grade-0 or 1 osteoarthritis at the latest follow-up were categorized into the non-progression group and the remaining 24 patients with Tönnis grade-2 or 3 osteoarthritis were categorized into the progression group. We evaluated patient characteristics as well as radiographic parameters, including the preoperative lateral center-edge angle, acetabular roof obliquity, and anterior center-edge angle. RESULTS: The mean duration of follow-up was 12.6 years (range, 10 to 19 years). On univariate analysis, body weight, body mass index, and all radiographic parameters indicating the severity of acetabular dysplasia significantly differed between the 2 groups. On multivariate analysis, both the anterior center-edge angle and acetabular roof obliquity were considered to be significant predictive factors for osteoarthritis progression. On receiver operating characteristic curve analysis, the cutoff values for the lateral center-edge angle, acetabular roof obliquity, and anterior center-edge angle were 15.5°, 15.5°, and 12.5°, respectively. CONCLUSIONS: In addition to the lateral center-edge angle and acetabular roof obliquity, we showed that the anterior center-edge angle is a possible prognostic predictor of osteoarthritis progression. These findings indicate that radiographic parameters based on the false-profile view are relevant. Additionally, patients with an anterior center-edge angle of ≤12.5° and a lateral center-edge angle of ≤15.5° seem to be at higher risk of osteoarthritis progression over a 10-year period. LEVEL OF EVIDENCE: Prognostic Level IV. See Instructions for Authors for a complete description of levels of evidence.

Osteocyte regulation of orthodontic force-mediated tooth movement via RANKL expression.

Orthodontic tooth movement is achieved by the remodeling of the alveolar bone surrounding roots of teeth. Upon the application of orthodontic force, osteoclastic bone resorption occurs on the compression side of alveolar bone, towards which the teeth are driven. However, the molecular basis for the regulatory mechanisms underlying alveolar bone remodeling has not been sufficiently elucidated. Osteoclastogenesis is regulated by receptor activator of nuclear factor-κB ligand (RANKL), which is postulated to be expressed by the cells surrounding the tooth roots. Here, we show that osteocytes are the critical source of RANKL in alveolar bone remodeling during orthodontic tooth movement. Using a newly established method for the isolation of periodontal tissue component cells from alveolar bone, we found that osteocytes expressed a much higher amount of RANKL than other cells did in periodontal tissue. The critical role of osteocyte-derived RANKL was confirmed by the reduction of orthodontic tooth movement in mice specifically lacking RANKL in osteocytes. Thus, we provide in vivo evidence for the key role of osteocyte-derived RANKL in alveolar bone remodeling, establishing a molecular basis for orthodontic force-mediated bone resorption.