RESUMEN
Migration and homing of immune cells are critical for immune surveillance. Trafficking is mediated by combinations of adhesion and chemokine receptors that guide immune cells, in response to chemokine signals, to specific locations within tissues and the lymphatic system to support tissue-localized immune reactions and systemic immunity1,2. Here we show that disruption of leukaemia inhibitory factor (LIF) production from group 2 innate lymphoid cells (ILC2s) prevents immune cells leaving the lungs to migrate to the lymph nodes (LNs). In the absence of LIF, viral infection leads to plasmacytoid dendritic cells (pDCs) becoming retained in the lungs where they improve tissue-localized, antiviral immunity, whereas chronic pulmonary allergen challenge leads to marked immune cell accumulation and the formation of tertiary lymphoid structures in the lung. In both cases immune cells fail to migrate to the lymphatics, leading to highly compromised LN reactions. Mechanistically, ILC2-derived LIF induces the production of the chemokine CCL21 from lymphatic endothelial cells lining the pulmonary lymphatic vessels, thus licensing the homing of CCR7+ immune cells (including dendritic cells) to LNs. Consequently, ILC2-derived LIF dictates the egress of immune cells from the lungs to regulate tissue-localized versus systemic immunity and the balance between allergen and viral responsiveness in the lungs.
Asunto(s)
Movimiento Celular , Quimiocina CCL21 , Células Dendríticas , Inmunidad Innata , Factor Inhibidor de Leucemia , Pulmón , Ganglios Linfáticos , Linfocitos , Ratones , Animales , Células Dendríticas/inmunología , Ganglios Linfáticos/inmunología , Pulmón/inmunología , Pulmón/virología , Inmunidad Innata/inmunología , Quimiocina CCL21/metabolismo , Quimiocina CCL21/inmunología , Factor Inhibidor de Leucemia/metabolismo , Factor Inhibidor de Leucemia/inmunología , Movimiento Celular/inmunología , Femenino , Linfocitos/inmunología , Linfocitos/citología , Masculino , Receptores CCR7/metabolismo , Receptores CCR7/inmunología , Ratones Endogámicos C57BL , Alérgenos/inmunología , Células Endoteliales/inmunología , Vasos Linfáticos/inmunologíaRESUMEN
Innate lymphoid cells (ILCs) and adaptive T lymphocytes promote tissue homeostasis and protective immune responses. Their production depends on the transcription factor GATA3, which is further elevated specifically in ILC2s and T helper 2 cells to drive type-2 immunity during tissue repair, allergic disorders, and anti-helminth immunity. The control of this crucial up-regulation is poorly understood. Using CRISPR screens in ILCs we identified previously unappreciated myocyte-specific enhancer factor 2d (Mef2d)-mediated regulation of GATA3-dependent type-2 lymphocyte differentiation. Mef2d-deletion from ILC2s and/or T cells specifically protected against an allergen lung challenge. Mef2d repressed Regnase-1 endonuclease expression to enhance IL-33 receptor production and IL-33 signaling and acted downstream of calcium-mediated signaling to translocate NFAT1 to the nucleus to promote type-2 cytokine-mediated immunity.
Asunto(s)
Factor de Transcripción GATA3 , Inmunidad Innata , Interleucina-33 , Factores de Transcripción MEF2 , Factores de Transcripción NFATC , Neumonía , Células Th2 , Animales , Ratones , Factores de Transcripción MEF2/metabolismo , Factores de Transcripción MEF2/genética , Células Th2/inmunología , Interleucina-33/metabolismo , Factores de Transcripción NFATC/metabolismo , Neumonía/inmunología , Factor de Transcripción GATA3/metabolismo , Factor de Transcripción GATA3/genética , Ratones Endogámicos C57BL , Diferenciación Celular , Señalización del Calcio , Hipersensibilidad/inmunología , Pulmón/inmunología , Alérgenos/inmunología , Linfocitos/inmunología , Proteína 1 Similar al Receptor de Interleucina-1RESUMEN
Regulatory T cells (Tregs) control adaptive immunity and restrain type 2 inflammation in allergic disease. Interleukin-33 promotes the expansion of tissue-resident Tregs and group 2 innate lymphoid cells (ILC2s); however, how Tregs locally coordinate their function within the inflammatory niche is not understood. Here, we show that ILC2s are critical orchestrators of Treg function. Using spatial, cellular, and molecular profiling of the type 2 inflamed niche, we found that ILC2s and Tregs engage in a direct (OX40L-OX40) and chemotaxis-dependent (CCL1-CCR8) cellular dialogue that enforces the local accumulation of Gata3high Tregs, which are transcriptionally and functionally adapted to the type 2 environment. Genetic interruption of ILC2-Treg communication resulted in uncontrolled type 2 lung inflammation after allergen exposure. Mechanistically, we found that Gata3high Tregs can modulate the local bioavailability of the costimulatory molecule OX40L, which subsequently controlled effector memory T helper 2 cell numbers. Hence, ILC2-Treg interactions represent a critical feedback mechanism to control adaptive type 2 immunity.