[Clinical manifestations and neuroimaging characteristics of children with moyamoya disease].

OBJECTIVE: To study the clinical manifestations and neuroimaging characteristics of pediatric moyamoya disease. METHODS: The clinical data of 17 children with moyamoya disease were retrospectively studied. RESULTS: The onset age was between 3 and 14 years. The main clinical manifestations included motor weakness of extremities or hemiplegia, sensory disturbance and headache. Cranial CT or/and MRI examinations predominately showed cerebral infarct. Magnetic resonance angiography (MRA) and digital subtraction angiography (DSA) showed stenosis or occlusion at the terminus of the siphon portions of internal carotid arteries and proximal portions of anterior or middle cerebral arteries, and abnormal vascular networks at the base of brain. CONCLUSIONS: Cerebral ischemia is main clinical manifestations in children with moyamoya disease, presenting motor weakness of extremities or hemiplegia, sensory disturbance and headache. DSA is essential to the diagnosis of the disease.

[The study of primary culture methods for hippocampal neurons of newborn rats].

AIM: To compare three different methods for neuron culture, so as to provide a culture technique with higher neuron purity and survival rate. METHODS: Neurons in hippocampal region of newborn SD rats were cultured by common culture method, cytosine arabinoside (Ara-c)-supplementing method, Ara-c and nerve growth factor (NGF)-supplementing method. Then morphology of neurons was observed under microscope. The survival rates of the neurons at different culture times were compared by inverted microscope observation and MTT colorimetry. The purity of neurons cultured by 3 methods was detected by immunocytochemical staining. RESULTS: Neurons cultured by Ara-c and NGF-supplementing method grew well. The purity and survival rate of neurons cultured by Ara-c and NGF-supplementing method was highest. CONCLUSION: Ara-c and NGF-supplementing method is a good method for neuron culture.