RESUMEN
Nocardial conjunctivitis associated with silicone tubing is an extremely rare finding. The authors present a case of a 52-year-old woman with previous dacryocystorhinostomy and silicone tube placement 3 years prior who presented with OD redness and discharge for 1 week. On examination, the patient was noted to have mucoid discharge and crusting surrounding the silicone tube. The tube debris was sampled, and the culture was positive for Nocardia nova complex sensitive to trimethoprim/sulfamethoxazole and amikacin. Silicone tube colonization and N. nova complex conjunctivitis are both rare but should be considered in the differential diagnosis of patients with indwelling silicone tubes presenting with chronic conjunctivitis resistant to fluoroquinolones and tobramycin.
Asunto(s)
Conjuntivitis Bacteriana/microbiología , Infecciones Bacterianas del Ojo/microbiología , Nocardiosis/microbiología , Nocardia/aislamiento & purificación , Infecciones Relacionadas con Prótesis/microbiología , Elastómeros de Silicona , Stents/microbiología , Antibacterianos/uso terapéutico , Enfermedad Crónica , Conjuntivitis Bacteriana/diagnóstico , Conjuntivitis Bacteriana/tratamiento farmacológico , Dacriocistorrinostomía/instrumentación , Infecciones Bacterianas del Ojo/diagnóstico , Infecciones Bacterianas del Ojo/tratamiento farmacológico , Femenino , Humanos , Intubación/instrumentación , Pruebas de Sensibilidad Microbiana , Persona de Mediana Edad , Nocardiosis/diagnóstico , Nocardiosis/tratamiento farmacológico , Infecciones Relacionadas con Prótesis/diagnóstico , Infecciones Relacionadas con Prótesis/tratamiento farmacológico , Combinación Trimetoprim y Sulfametoxazol/uso terapéuticoRESUMEN
Preparation of a random-sequence DNA pool is presented. The degree of randomization and the length of the random sequence are discussed, as is synthesis of the pool using a DNA synthesizer or via commercial synthesis companies. Purification of a single-stranded pool and conversion to a double-stranded pool are presented as step-by-step protocols. Support protocols describe determination of the complexity and skewing of the pool, and optimization of amplification conditions.
Asunto(s)
ADN de Cadena Simple/síntesis química , ADN/metabolismo , Evolución Molecular Dirigida , Biología Molecular/métodos , Distribución Aleatoria , Técnica SELEX de Producción de Aptámeros/métodos , ADN/aislamiento & purificación , ADN de Cadena Simple/aislamiento & purificaciónRESUMEN
Preparation of a random-sequence DNA pool is presented. The degree of randomization and the length of the random sequence are discussed, as is synthesis of the pool using a DNA synthesizer or via commercial synthesis companies. Purification of a single-stranded pool and conversion to a double-stranded pool are presented as step-by-step protocols. Support protocols describe determination of the complexity and skewing of the pool, and optimization of amplification conditions.