Shoals in troubled waters? The impact of rising temperatures on metabolism, foraging, and shoaling behavior in mixed-species shoals.

Rising water temperatures across aquatic habitats, in the current global climate change scenario, can directly affect metabolism and food intake in fish species. This can potentially alter their physiological, behavioral, and shoaling properties. In the current study, we examined the effects of high temperatures on metabolism, foraging, and shoaling in tropical fish. Mixed-species (comprising flying barbs, zebrafish, and gambusia) and single-species (flying barbs and zebrafish) shoals were conditioned for 45 days to three kinds of temperature regimes: the current temperature regime (CTR), in which shoals were maintained at water temperature of 24°C (i.e., the current mean temperature of their habitat), the predicted temperature regime (PTR) at 31°C (i.e., simulating conditions projected for their habitat in 2100), and the dynamic temperature regime (DTR), which experienced daily temperature fluctuations between 24 and 31°C (i.e., resembling rapid temperature changes expected in their natural environments). We found species-specific responses to these temperature regimes. Flying barbs exhibited significantly lower body weight at PTR but maintained consistent muscle glycogen content across all temperature regimes. In contrast, zebrafish and gambusia displayed significantly elevated muscle glycogen content at PTR, with similar body weights across all three temperature regimes. Cohesion within flying barb shoals and cohesion/polarization in mixed-species shoals decreased significantly at PTR. Shoals exposed to DTR exhibited intermediate characteristics between those conditioned to CTR and PTR, suggesting that shoals may be less impacted by dynamic temperatures compared to prolonged high temperatures. This study highlights species-specific metabolic responses to temperature changes and their potential implications for larger-scale shoal properties.

The impact of predators and vegetation on shoaling in wild zebrafish.

In their natural habitats, animals experience multiple ecological factors and regulate their social responses accordingly. To unravel the impact of two ecological factors on the immediate behavioural response of groups, we conducted experiments on wild zebrafish shoals in arenas with vegetation, predator cues, and both factors simultaneously or neither (control treatments). Analysis of 297 trials revealed that while shoals formed significantly larger subgroups in the presence of predator cues, their subgroup size was comparable to control treatments when they faced predator cues and vegetation. Shoals were highly polarized in open arenas, in the absence of either ecological factors and in the presence of predator cues (with/without vegetation). The presence of vegetation alone, however, significantly reduced shoal polarization. Furthermore, food intake was significantly reduced when predator cues and/or vegetation were present. Tracking individuals revealed that (i) individuals within shoals receiving predator cues had a significantly higher probability to continue being in a group compared with control treatments and (ii) individuals occupying the front positions deviated less from their median position within a shoal as compared with other individuals regardless of predator cues. The adaptability of animals depends on behavioural responses to changing environments, making this study significant in the context of environmental changes.

What drives mixed-species shoaling among wild zebrafish? The roles of predators, food access, abundance of conspecifics and familiarity.

Mixed-species groups occur across a wide range of faunal communities and provide several benefits to members. While zebrafish have often been observed to form mixed-species shoals with coexisting species, the factors determining their occurrence are not yet fully understood. Shoals comprising zebrafish (Danio rerio), flying barbs (Esomus danricus), and whitespots (Aplocheilus panchax) were collected from a stagnant canal at Haringhata (West Bengal, India), and using laboratory-based experiments, we deciphered likely drivers of mixed-species shoaling among zebrafish. Experiments assessing foraging efficiency revealed that the amount of food consumed by individual zebrafish in mixed shoals was comparable to the amount consumed by these individuals in conspecific shoals. Within mixed-species shoals, zebrafish individuals, despite being smaller than the other species, consumed a comparable amount of food as the other species. Shoal choice experiments revealed that under predator risk, zebrafish associate more with mixed shoals and showed comparable associations to shoals differing in the abundance of conspecifics. Furthermore, zebrafish preferred associating with familiar conspecifics over unfamiliar mixed and unfamiliar conspecific shoals. Therefore, equitable food consumption in mixed shoals, greater association with mixed shoals in the presence of predators, and familiarity were important in driving zebrafish towards mixed-species shoaling. This article has an associated First Person interview with the first author of the paper.

Temporal Patterns in Interactions Across Same- and Mixed-Sex Wild Zebrafish Dyads.

Animals communicate with each other through a variety of behavioral interactions, many of which are often complex due to the interplay of several ecological factors. Observations on dyadic interactions can help throw light on the more complex interactions observed among group living organisms and can help in understanding mechanisms of behaviors related to mating strategies, dominance hierarchies, and decision-making. This study focused on the assessment of several generally observed interactions among dyads of different sexes (female-female, male-male, and male-female) in wild zebrafish (Danio rerio). Temporal dynamics of these interactive behaviors were observed in 45 dyads across 3 time intervals of the day. We used generalized linear mixed models to investigate the effect of time, sex of dyad, and their interaction on specific behaviors. While the frequency of occurrence of some behaviors showed clear variation across time intervals of the day, these were further found to depend on the composition of the dyad. Contrary to previous reports, we found that same-sex dyads are equally aggressive and aggressive interactions did not vary temporally. Mating-associated interactions, as expected, were significantly higher in mixed-sex dyads and declined significantly from early morning to afternoon. Interestingly, we also found some mating-associated interactions in same-sex dyads. A fine line exists between social and mating-associated interactions in many organisms and so we speculate that these interactions could also be social interactions and not mating-related behavior. Our findings shed light on complex interactive behaviors among zebrafish, that are likely to be affected by time as well as sex composition of interacting individuals and thus has important implications for groups varying in sex ratios in the wild.

Policy capacities and effective policy design: a review.

Effectiveness has been understood at three levels of analysis in the scholarly study of policy design. The first is at the systemic level indicating what entails effective formulation environments or spaces making them conducive to successful design. The second reflects more program level concerns, surrounding how policy tool portfolios or mixes can be effectively constructed to address complex policy objectives. The third is a more specific instrument level, focusing on what accounts for and constitutes the effectiveness of particular types of policy tools. Undergirding these three levels of analysis are comparative research concerns that concentrate on the capacities of government and political actors to devise and implement effective designs. This paper presents a systematic review of a largely scattered yet quickly burgeoning body of knowledge in the policy sciences, which broadly asks what capacities engender effectiveness at the multiple levels of policy design? The findings bring to light lessons about design effectiveness at the level of formulation spaces, policy mixes and policy programs. Further, this review points to a future research agenda for design studies that is sensitive to the relative orders of policy capacity, temporality and complementarities between the various dimensions of policy capacity.

Förster Resonance Energy Transfer from Carbon Nanoparticles to a DNA-Bound Compound: A Method to Detect the Nature of Binding.

A drug molecule can bind in various orientations to a DNA strand. Nature of the binding decides the functionality and efficacy of the drug. To innovate a new method to detect the nature of binding of a drug to DNA strands, herein we have used the dipole-dipole interaction driven Förster resonance energy transfer (FRET) between carbon nanoparticles (CNPs) and a DNA-bound small molecule, (E)-3-ethyl-2-(4-(pyrrolidin-1-yl)styryl)benzo[d]thiazol-3-ium (EPSBT), which belongs to the hemicyanine family and binds typically to the minor groove of a DNA duplex. EPSBT was designed to obtain appreciable fluorescence quantum yield, which constructed an efficient FRET pair with the synthesized CNPs. The tested compound prefers the thymine nucleobase to bind to the DNA strand. Orientation of its dipole on attachment to the DNA strand and the donor-acceptor distance dictate the FRET efficiency with the CNPs. The results provided a precise estimation of the nature of binding of EPSBT to the DNA backbone and, hence, supposedly will help in deciding the functional efficacy.