Breeding program in rehabilitated bottlenose dolphins (Tursiops truncatus gephyreus) from the Southwestern Atlantic Ocean.

Tursiops truncatus gephyreus is only found in the inshore waters of the Southwestern Atlantic Ocean. They are organized in small socially, structured groups, which lead to low genetic variability and high anthropogenic threats. Currently, the information about their reproductive biology and management is still insufficient. Thus, this study aims to present novel information recorded for 20 years regarding reproductive parameters in a small population of captive T. t. gephyreus. Three of the animals involved were found stranded in La Plata River estuary and, the other two were born at the oceanarium. Thirteen semen evaluations showed high-quality semen with a low percentage of sperm abnormalities. Twelve pregnancies and parturitions were observed. There was a clear calving seasonality during austral spring and summer. The duration of phase-2 of labor was approximately 80 min, being the caudal presentation the most frequent. The average inter-birth interval (IBI) was about 33 months. This is the first report that closely monitored parturition, IBI, and seminal evaluation in this species. Information gathered during this program will allow the development of adequate conservation plans for free-ranging populations.

Serum progesterone concentration, volume, and apoptosis of corpora lutea in early, middle and late diestrus in the bitch.

The aim was to study number, volume, apoptosis of corpora lutea (CL), and serum P4 concentrations in early, middle, and late diestrus of dogs. Thirty-six bitches were ovari-hysterectomized (OVX): Early Diestrus (Group [G]1; OVX 20 days after end of estrus [DEH]); Mid-diestrus (GII; OVX between 21 and 40 days after DEH), and Late-diestrus (GIII; OVX between 41 and 60 days after DEH). Before OVX a blood sample was collected to quantify P4. After OVX, the number of CL (NCL) was recorded, CL measured using both ultrasonography (US) and caliper (CAL), and the volume (mm3) was calculated. Based on abundances of caspase-3, apoptotic luteal cells were detected. Bitches in early-diestrus had greater P4 concentrations than bitches in mid- and late-diestrus (23.52 ±â€¯3.78 and 10.86 ±â€¯3.88 ng/mL; P < 0.05). The NCL, cumulative USCLV, and CALCLV were similar among diestrus stages (P > 0.30). Bitches with CL (≥5) had twice the serum P4 concentrations as bitches with CL1-2 and CL3-4 (22.71 ±â€¯3.70 and 10.97 ±â€¯4.06 ng/mL; P < 0.05). There were correlations between P4 concentrations with USCLV, CALCLV, and NCL (r = 0.64, r = 0.67, r = 0.44; P < 0.0001). When serum P4 concentrations were adjusted for stages of diestrus, however, there were only correlations during early diestrus. The percentage of apoptotic cells was greater in GIII compared with GI and GII (13.75 ±â€¯2.26 % compared with 4.5 ±â€¯0.68 % and 4.6 ±â€¯1.5 %, respectively; P < 0.05). As days of diestrus increased, number of apoptotic cells increased, and serum P4 concentrations decreased.

Relationship between semen quality and seminal plasma cholesterol, triacylglycerols and proteins in the domestic cat.

OBJECTIVES: The current study aimed to evaluate the relationship between specific seminal plasma components - cholesterol (CHOL), triacylglycerols (TAG) and total protein (PROT) concentrations - and semen quality in cats. A further aim was to determine the relationship between specific seminal protein bands and semen quality. METHODS: Thirteen toms, 2-5 years of age, were included. Semen collection was performed by electroejaculation every 4 weeks. Fifty-eight ejaculates were assessed for motility, velocity, volume, sperm concentration, total sperm count, viability, acrosome integrity, plasma membrane integrity and sperm morphology. Samples were divided into two groups: good semen quality (GSQ) and poor semen quality (PSQ). After evaluation, seminal plasma was separated from the sperm by centrifugation and stored at -20°C. CHOL, TAG and PROT concentrations were then assessed and seminal plasma protein profile was determined by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). RESULTS: Seminal plasma CHOL and TAG concentrations, motility, velocity, sperm concentration, total sperm count and sperm morphology were significantly higher in GSQ cats compared with PSQ cats (P <0.01). Moreover, seminal plasma SDS-PAGE analysis showed an identifiable extra band exclusively in the GSQ group. CONCLUSIONS AND RELEVANCE: Data obtained in this study showed that seminal plasma CHOL and TAG concentrations and specific protein bands could be used to improve semen evaluation in toms. In this sense, the 14 kDa protein band could be a valuable marker for semen quality in the cat and should be further investigated. However, more studies are necessary to determine its relationship with fertility.

Reestablishment of sperm quality after long-term deslorelin suppression in tomcats.

The aim of the study was to determine the time after treatment with a 4.7 mg deslorelin implant until Tomcat spermatogenesis activity was restored, and seminal parameters reached pre-implant values. Tomcats (n = 6) were randomly assigned to one of two treatments. Three cats (n = 3) received a deslorelin implant (4.7 mG; Suprelorin®, Virbac, France) in the interscapular subcutaneous region whereas three (n = 3) received no implant and served as control group. Semen samples were collected by electroejaculation every 4 wk from 3 mo before treatment (pretreatment samples) until reestablishment of pre-treatment sperm quality, 32 mo post-implant insertion (PI). Each semen sample was assessed for motility, velocity, concentration, total sperm count, viability, acrosome integrity, plasma membrane integrity and sperm morphology. After semen collection, testicular volume and presence/absence of penile spines were recorded. Additionally, blood samples were taken to measure testosterone concentration. An increase in sperm concentration and total sperm count was present 1 mo PI despite of an abrupt decrease in serum testosterone concentrations after 2-4 weeks. This initial stimulatory effect was followed by a decrease in seminal parameters, reduction of testicular volume and disappearance of penile spines 2 mo PI. A single Suprelorin® 4.7 mg implant suppressed sperm production for 22-25 months. No clinically side effect was observed during the study period. All toms returned to their initial seminal quality 23-28 months after treatment. Therefore, we conclude that Suprelorin® 4.7 mg is a safe option for reversible reproduction control during long periods in tomcats.

Cloprostenol treatment of feline open-cervix pyometra.

Treatment with cloprostenol, a prostaglandin synthetic analogue, was evaluated in five queens with open-cervix pyometra. Cloprostenol was administered (5 µg/kg body weight SC) on 3 consecutive days and amoxicillin (20 mg/kg body weight IM) on 7 consecutive days. Transient post-injection reactions caused by cloprostenol administration included diarrhea, vomiting and vocalizations. Reactions began as quickly as 10 mins after cloprostenol administration and lasted as long as 30 mins. All queens improved clinically after cloprostenol treatment and remained healthy until the end of the study, 1 year after treatment. All queens resumed normal estrous cycles without further treatment and two (40%) delivered a normal litter. In conclusion, use of cloprostenol is an acceptable treatment for open-cervix pyometra in queens.