Genomic analyses in African populations identify novel risk loci for cleft palate.

Orofacial clefts are common developmental disorders that pose significant clinical, economical and psychological problems. We conducted genome-wide association analyses for cleft palate only (CPO) and cleft lip with or without palate (CL/P) with ~17 million markers in sub-Saharan Africans. After replication and combined analyses, we identified novel loci for CPO at or near genome-wide significance on chromosomes 2 (near CTNNA2) and 19 (near SULT2A1). In situ hybridization of Sult2a1 in mice showed expression of SULT2A1 in mesenchymal cells in palate, palatal rugae and palatal epithelium in the fused palate. The previously reported 8q24 was the most significant locus for CL/P in our study, and we replicated several previously reported loci including PAX7 and VAX1.

Oral Health-Related Quality of Life of Children Born With Orofacial Clefts in Ethiopia and Their Parents.

OBJECTIVE: To assess the oral health-related quality of life (OH-RQoL) using a translated standardized measure in an understudied population of Ethiopian children born with orofacial clefts (OFCs) and their parents. METHODS: Using a descriptive study design, we assessed the OH-RQoL of 41 patients with OFCs between the ages of 8 and 17 years and their parents. Participants received multidisciplinary cleft care from 2008 to 2016. They completed an Amharic translation of the Child Oral Health Impact Profile (COHIP). RESULTS: There was strong internal reliability with the translated COHIP for parents and patients. Parents' COHIP scores ranged from 67 to 186, and patients' scores were 78 to 190. The mean for patients and parents was 155, indicating good OH-RQoL. CONCLUSION: The Amharic translation of the COHIP appears appropriate for use with families in Ethiopia. Both parents and patients reported OH-RQoL at similar levels as other international populations.

Novel GREM1 Variations in Sub-Saharan African Patients With Cleft Lip and/or Cleft Palate.

OBJECTIVE: Cleft lip and/or cleft palate (CL/P) are congenital anomalies of the face and have multifactorial etiology, with both environmental and genetic risk factors playing crucial roles. Though at least 40 loci have attained genomewide significant association with nonsyndromic CL/P, these loci largely reside in noncoding regions of the human genome, and subsequent resequencing studies of neighboring candidate genes have revealed only a limited number of etiologic coding variants. The present study was conducted to identify etiologic coding variants in GREM1, a locus that has been shown to be largely associated with cleft of both lip and soft palate. PATIENTS AND METHOD: We resequenced DNA from 397 sub-Saharan Africans with CL/P and 192 controls using Sanger sequencing. Following analyses of the sequence data, we observed 2 novel coding variants in GREM1. These variants were not found in the 192 African controls and have never been previously reported in any public genetic variant database that includes more than 5000 combined African and African American controls or from the CL/P literature. RESULTS: The novel variants include p.Pro164Ser in an individual with soft palate cleft only and p.Gly61Asp in an individual with bilateral cleft lip and palate. The proband with the p.Gly61Asp GREM1 variant is a van der Woude (VWS) case who also has an etiologic variant in IRF6 gene. CONCLUSION: Our study demonstrated that there is low number of etiologic coding variants in GREM1, confirming earlier suggestions that variants in regulatory elements may largely account for the association between this locus and CL/P.

Traumatic dental injuries in children with special health care needs.

BACKGROUND/AIM: Children with special healthcare needs (CSHCN) are at an increased risk for traumatic dental injuries (TDI) due to unique predisposing factors; moreover, their access to dental care is compromised. In Jordan, there is a need to study TDI among CSHCN to highlight their dental care needs. The aim of this study was to identify the prevalence, types, possible risk factors associated with TDI, and treatment-seeking behavior in CSHCN in Jordan. MATERIAL AND METHODS: Children (n=959) were examined in schools/centers for CSHCN, and compared to a healthy age- and gender-matched control group. Data concerning demographics, types of trauma, risk factors associated with TDI, and treatment-seeking behavior were collected. Data were analyzed using SPSS for descriptive and bivariate analyzes. Significance level was set at P≤.05. RESULTS: Prevalence of TDI in the study group (age, 11.76±4.2 years) and control group (age 11.70±4.2 years) was (83 of 959, 8.7%) and (42 of 1010, 4.1%), respectively. TDI prevalence was highest in children with multiple disabilities (14.0%), followed by intellectual disabilities (13.1%), and cerebral palsy (12.2%). The most common type of TDI was an uncomplicated crown fracture (91.0%). Increased overjet and incompetent lips were significant risk factors associated with TDI. Reasons for not seeking treatment in the study vs control group included parental attitude and lack of dental awareness (68.1% vs 60%), difficulties getting an appointment and availability of dental clinics willing to see CSHCN (36.2% vs 0%), P≤.01, and financial reasons (31.9% vs 40%). CONCLUSIONS: Prevalence of TDI was higher in CSHCN, and associated with increased overjet and incompetent lips. Uncomplicated crown fracture was the most common injury. In both groups, the main reason for not seeking treatment was lack of dental awareness among parents/caregivers; however, difficulties in getting an appointment and availability of dental clinics willing to see children were more prominent in the CSHCN group.

Success rates of Hall technique crowns in primary molars: a retrospective pilot study.

The purpose of this retrospective observational study was to assess success rates, both clinical and radiographic, of stainless steel crowns (SSCs) placed on primary molars using the Hall technique. A retrospective analysis was performed on recorded data of patients with any primary molar treated with an SSC that was placed using the Hall technique at the University of Iowa College of Dentistry during 2011-2015. The primary outcome measure was the success or failure of the SSCs placed with the Hall technique. These outcomes were categorized as either clinical and radiographic success or failure. Clinical failure was defined as the need for pulp therapy or extraction following crown placement. Radiographic failure was defined as the presence of any pathological condition-including external or internal root resorption, bifurcation radiolucency, widened periodontal ligament, or ectopic eruption of permanent first molar adjacent to the Hall crown-following crown placement. Records indicated that 100 boys received a total of 179 crowns (61.1% of all Hall crowns placed), and 64 girls received 114 crowns. The mean age of the patients was 5.1 years (SD, 2.4 years). Of 293 SSCs included in the study, 180 received at least 1 follow-up examination after a mean of 9.9 months (SD, 6.5 months). At the first follow-up visit, 178 (98.9%) of 180 SSCs placed using the Hall technique were clinically successful. Of 87 crowns with radiographs available, 85 (97.7%) were radiographically successful. At the second follow-up visit (after a mean of 20.1 months), 74 of 76 (97.4%) were rated as clinically successful, and 37 of 39 (94.9%) were radiographically successful. Results of this study provide evidence of high clinical and radiographic success rates for SSCs placed on primary molars with the Hall technique.

Maximum occusal bite force for children in different dentition stages.

This study was carried out to record maximum occlusal bite force (MOBF) among different dentition stages in children and to study the relation of occlusal bite force to weight, height, and gender. A total of 1011 children (500 females and 511 males) aged from 3 to 18 years were examined. The subjects were divided into 5 groups according to their dentition stage as the following:- early primary dentition stage (100 males and 100 females, average age was 3.37 ± 0.23 years), late primary dentition stage (104 males and 100 females, average age was 5.86 ± 1.15 years), early mixed dentition stage (100 males and 100 females, average age was 8.15 ± 0.67 years), late mixed dentition stage (100 males and 100 females, average age was 9.97 ± 0.86 years ), and permanent dentition stage (107 males and 100 females, average age was 14.03 ± 2.14 years). Occlusal bite force was measured using a hydraulic occlusal force gauge. The means of MOBF for the different dentition stages were:- 176 N in early primary stage, 240 N in late primary stage, 289 N in early mixed stage, 433 N in late mixed stage, and 527 N in the permanent dentition stage, respectively. Gender differences were detected in groups 2, 3 and 4. Height and age significantly correlated with the MOBF in all dentition stage groups except group 1. In conclusion, the MOBF increased with age. Age, gender, and height were significant predictors of the MOBF.

Clinical Versus Radiographic Caries Diagnosis in Primary Tooth Approximal Surfaces.

Purpose: The purpose of this study was to compare approximal carious lesions in dentin diagnosed by clinical examination with those diagnosed radiographically in high caries-risk children. Methods: Eighty-four Amish two- to 11-year-olds were evaluated clinically by calibrated examiners using standard epidemiological criteria and radiographically using standard criteria as part of baseline examinations for a two-year pilot clinical trial. Approximal carious surfaces diagnosed by clinical examination were compared to those diagnosed radiographically to determine the number of lesions misdiagnosed by clinical examination alone. Comparisons between the number of lesions detected by clinical exam and radiographic exam were made using McNemar's test. Results: Among 124 anterior approximal tooth surfaces, clinical examination alone identified 61 lesions (49.2 percent), while radiograph examination identified 72 lesions (58.1 percent); thus, nine percent of lesions were misdiagnosed by clinical examination alone. For the 412 posterior approximal tooth surfaces included in the study, 101 lesions (24.5 percent) were identified by clinical examination, whereas 229 lesions (55.6 percent) were identified via radiographic examination. Thus, 128 (31.1 percent) were misdiagnosed by clinical examination alone. Conclusions: Clinical examinations without radiographs significantly underestimate caries prevalence. Therefore, the use of radiographs should be encouraged in epidemiological studies and clinical trials.

Dentists' involvement in identification and reporting of child physical abuse: Jordan as a case study.

OBJECTIVES: The objectives of this study were to assess the knowledge, attitude, and experience among Jordanian dentists regarding child abuse, and to explore the factors that affect their hesitation to report any suspected cases. MATERIALS AND METHODS: A self-administered structured questionnaire was sent randomly to 500 Jordanian dentists. It investigated dentists' knowledge, attitude, and experience in recognizing and reporting child abuse cases. It also investigated several factors associated with dentists' hesitation to report suspected cases of child physical abuse. RESULTS: The response rate was 68%. More dentists were aware of their ethical obligations (80%) than their legal responsibilities (71%) to report child abuse cases. One-third of the dentists knew where to report suspected cases. Although 42% of dentists suspected cases of child abuse, only 20% of them reported these cases. The most frequently cited reasons for hesitation to report such cases were lack of history (76%), uncertainty about diagnosis (73%), and possible consequences on the child (66%). Reporting was significantly associated with suspicion of child abuse cases, as well as the belief of legal responsibilities. CONCLUSION: There was a low reporting rate of child abuse among Jordanian dentists. They lack the adequate knowledge about recognition and reporting issues of suspected cases.

A Preexperimental Study to Assess the Impact of an Interdisciplinary Educational Intervention on Nurses' Knowledge of Perinatal and Infant Oral Health Care.

OBJECTIVE: The objective of this study was to assess the impact of an interdisciplinary educational intervention on the knowledge of nursing practitioners regarding perinatal and infant oral health (PIOH) care. MATERIALS AND METHODS: This was a preexperimental study conducted among nursing practitioners in Lagos, Nigeria. Participants received hands-on training and didactic lectures, which included dental caries etiology and risk factors; oral hygiene and dietary education; teething and its management; dental trauma and its prevention; nonnutritive habits; screening, referrals, and counseling; and fluoride varnish application. Knowledge of the trainees was assessed using pre- and posttest questionnaires. Level of statistical significance was set at P < 0.05. RESULTS: Overall, 110 nurses participated in the study with a mean age of 40.9 ± 10.8 years; 106 (96.4%) were females. Approximately 88% of the participants had not received formal training on PIOH. The baseline mean scores of the participants' knowledge on oral hygiene, teething, trauma, caries, and oral habits were 4.31 ± 1.9, 9.84 ± 2.6, 2.59 ± 1.7, 4.24 ± 1.8, and 1.45 ± 0.6, respectively; this increased significantly (P < 0.001) following the educational intervention with posttest mean scores as 7.58 ± 0.8, 11.79 ± 1.3, 4.34 ± 1.9, 6.19 ± 1.8, and 1.82 ± 0.4 and six-month evaluation scores as 6.21 ± 1.8,7 10.27 ± 3.1, 4.39 ± 1.5, 5.91 ± 1.8, and 1.79 ± 0.5, respectively. Overall posttest (31.4 ± 4.2) and six-month (28.6 ± 6.2) knowledge scores were significantly higher than the pretest values (22.4 ± 4.8, P < 0.001). At the six-month post-intervention survey, 84% of the nurses reported inclusion of PIOH education in their routine general health education sessions. CONCLUSION: There was a positive impact of the educational intervention as evidenced by an increase in the knowledge of the nurses on PIOH care and the inclusion of PIOH education in their general health education. A slight decline between posttest and six-month evaluation scores indicates a need for continuous education and evaluation.

Missense Pathogenic variants in KIF4A Affect Dental Morphogenesis Resulting in X-linked Taurodontism, Microdontia and Dens-Invaginatus.

The etiology of dental anomalies is multifactorial; and genetic and environmental factors that affect the dental lamina have been implicated. We investigated two families of European ancestry in which males were affected by taurodontism, microdontia and dens invaginatus. In both families, males were related to each other via unaffected females. A linkage analysis was conducted in a New Zealand family, followed by exome sequencing and focused analysis of the X-chromosome. In a US family, exome sequencing of the X-chromosome was followed by Sanger sequencing to conduct segregation analyses. We identified two independent missense variants in KIF4A that segregate in affected males and female carriers. The variant in a New Zealand family (p.Asp371His) predicts the substitution of a residue in the motor domain of the protein while the one in a US family (p.Arg771Lys) predicts the substitution of a residue in the domain that interacts with Protein Regulator of Cytokinesis 1 (PRC1). We demonstrated that the gene is expressed in the developing tooth bud during development, and that the p.Arg771Lys variant influences cell migration in an in vitro assay. These data implicate missense variations in KIF4A in a pathogenic mechanism that causes taurodontism, microdontia and dens invaginatus phenotypes.

Maximum occlusal bite force in pre-school children with different occlusal patterns.

BACKGROUND: This study was carried out to record maximum occlusal bite force (MOBF) in pre-school children with different occlusal patterns. MATERIAL AND METHODS: A randomly selected sample of 1085 kindergarten children in primary dentition stage were selected. The age of subjects ranged between 3-6 years (averaged 4.90 ± 0.86 years). The subjects were divided into 3 groups according to molar relationship; flush terminal (n=335; 165 males and 170 females), distal step (n=450; 200 males and 250 females), mesial step (n=300; 150 males and 150 females) molar relationship. Clinical examination involved the record of molar relationship, overjet, overbite and the presence of wearing facets. Occlusal bite force was measured using a hydraulic occlusal force gauge. RESULTS: The means of MOBF for the different occlusal relationship were: - 193.47N (± 60.98), 179.20N (±56.80) and 245.11N (±69.45) for flush terminal, mesial and distal step molar relationships, respectively. Significant differences between studied groups were detected (P<0.01; P<0.001). MOBF were higher in subjects with distal step molar relationship, increased overjet and increased overbite. Gender differences were detected in flush terminal and distal step molar relationships. CONCLUSIONS: MOBF was affected by the different occlusal relationships. Children with distal step and mesial step molar relationship had the highest and the lowest MOBF, respectively. MOBF was similar in children with/without wearing facets. Key words:Occlusal, Bite force, primary, dentition, Pre-school.

The role of PAX9 promoter gene polymorphisms in causing hypodontia: a study in the Jordanian population.

BACKGROUND: The congenital absence of one or few teeth, hypodontia, is considered one of the utmost dental ageneses in human beings. Several genes have been shown to be involved in the development of hypodontia such as paired box gene 9 (PAX9). The expression of PAX9 is controlled by several polymorphic elements in the promoter region of the gene on 14q13.3 locus. The aim of this study was to find any association between PAX9 c.-912T>C (rs2073247) and c.-1031G>A (rs2073244) promoter polymorphisms and the development of hypodontia among the Jordanian population. METHODS: Genotyping of the polymorphisms in 72 unrelated subjects with hypodontia was performed using PCR-restriction fragment length polymorphism (RFLP) technique and compared with that of 72 normal healthy unrelated control individuals. RESULTS: The hypodontia group had a significantly higher -1031GG genotype (P<0.01) and a significantly lower -912TC genotype (P<0.01) compared with the control group. The results suggest that the transcriptional activity of PAX9 gene is affected by polymorphisms in the promoter region of this gene and is associated with hypodontia phenotype. CONCLUSION: The rs2073247) and rs2073244 promoter polymorphisms of PAX9 might play a role in the development of hypodontia in the Jordanian population.

Dentists' intention to report suspected violence: a cross-sectional study in eight Arab countries.

OBJECTIVES: This study assessed dentists' intention in eight Arab countries to report suspected exposure to violence among patients and factors associated with this intention based on the theory of planned behaviour. METHODS: A cross-sectional study was conducted in 2016 including a convenience sample of dentists practising in public, private and academic sectors in Algeria, Egypt, Jordan, Kuwait, Libya, Palestine, Saudi Arabia and Yemen. Respondents answered a self-administered questionnaire collecting information about personal and professional background and perceived ability to identify victims of violence. The questionnaire assessed (on a scale from 1 to 10 using six negative statements) dentists' perception of healthcare system mandated reporting of suspected violence. Six statements were used to assess professional attitude towards reporting suspected violence. Logistic regression was used to assess the association between intention to report suspected violence and perceived ability, perception and attitude adjusting for confounders. RESULTS: The response rate was 65.2% (n=2936/4506) from general practitioners (70.9%) of mean age=31 years with 56.7% women. Of those, 68.8% intended to report and 52.2% considered themselves able to identify violence victims. The mean (SD) negative perception score=5.3/10 (2.1) and the mean (SD) professional attitude score=7.5/10 (1.9). In multivariate regression, intention to report was associated with professional attitude (OR 1.08, 95% CI 1.03 to 1.14), ability to identify violence victims (OR 1.76, 95% CI 1.45 to 2.12) and negative perception that reporting is not mandated (OR 0.89, 95% CI 0.85 to 0.94). Significant differences existed among countries in intention to report. CONCLUSION: Most dentists intended to report suspected violence and their intention could be explained by the theory of planned behaviour which offers a framework for professional development to support violence victims. Sharing of training resources, policies and guidelines is needed to ensure that practices similar to international guidelines are consistently adopted by dentists across Arab countries.

Identification of paternal uniparental disomy on chromosome 22 and a de novo deletion on chromosome 18 in individuals with orofacial clefts.

BACKGROUND: Orofacial clefts are the most common malformations of the head and neck region. Genetic and environmental factors have been implicated in the etiology of these traits. METHODS: We recently conducted genotyping of individuals from the African population using the multiethnic genotyping array (MEGA) to identify common genetic variation associated with nonsyndromic orofacial clefts. The data cleaning of this dataset allowed for screening of annotated sex versus genetic sex, confirmation of identify by descent and identification of large chromosomal anomalies. RESULTS: We identified the first reported orofacial cleft case associated with paternal uniparental disomy (patUPD) on chromosome 22. We also identified a de novo deletion on chromosome 18. In addition to chromosomal anomalies, we identified cases with molecular karyotypes suggesting Klinefelter syndrome, Turner syndrome and Triple X syndrome. CONCLUSION: Observations from our study support the need for genetic testing when clinically indicated in order to exclude chromosomal anomalies associated with clefting. The identification of these chromosomal anomalies and sex aneuploidies is important in genetic counseling for families that are at risk. Clinicians should share any identified genetic findings and place them in context for the families during routine clinical visits and evaluations.

Infection control knowledge and practices among dentists and dental nurses at a Jordanian University Teaching Center.

BACKGROUND: Information regarding compliance with infection control precautions in the dental settings in the Middle East is scant. OBJECTIVE: To examine the knowledge and practices in infection control among dental staff and dental nurses at a university teaching dental center. METHODS: A self-administered, confidential questionnaire concerning various aspects of infection control knowledge and practices was distributed to 48 dental staff and 28 dental nurses working at the Jordan University of Science and Technology Dental Teaching Center. RESULTS: Responses came from 37 (77%) dental teaching staff members and 23 (82%) dental nurses. Of the dental staff participants, 95% received hepatitis B immunization in comparison with 87% of the dental nurses. Dental nurses were more prone to percutaneous dental injuries (P < .05). In both groups, 100% reported routine wearing of gloves. Although the dental staff reported a statistically higher frequency of washing hands before gloving (46%), dental nurses reported a higher frequency of washing hands after removing the gloves (100%). Dental staff reported statistically higher frequency of routine mask use (43%) than dental nurses (30%). CONCLUSION: Compliance with recommended guidelines for control of cross infection varied among the 2 tested groups. A need exists for proper practice of infection control by both dental staff and dental nurses.

Ability of Pit and Fissure Sealant-containing Amorphous Calcium Phosphate to inhibit Enamel Demineralization.

AIM: To evaluate the effect of amorphous calcium phosphate (ACP)-containing pit and fissure sealant on inhibition of enamel demineralization in vitro. MATERIALS AND METHODS: Enamel specimens (n = 75) were prepared using freshly extracted noncarious human third molars. Box-shaped cavities (8 × 2 × 2 mm) on the buccal or lingual surfaces were prepared and restored with resin-based sealant (Concise™), ACP-containing sealant (Aegis®) or fluoride-containing sealant (Conseal-F™). The samples were acid challenged in a demineralizing solution of 50 mmol/l lactic acid at pH 5.0 for 4 days. The change in enamel microhardness (ASuH) was calculated. Data were analyzed using one-way analysis of variance (ANOVA) and Tukey's post hoc test. RESULTS: The mean SuH0 (±SD) (in Vicker's unit) prior to the acid challenge was: Concise™ (318.83 ± 33.86), Aegis® (331.03 ± 21.52), Conseal-F™ (310.12 ± 34.31). Following the acid challenge, the values dropped in all groups and ASuH (±SD) values were 269.17 ± 47.49, 151.39 ± 23.96 and 175.79 ± 32.39 respectively. CONCLUSION: The ACP-containing pit and fissure sealant has the potential to inhibit enamel demineralization. How to cite this article: Zawaideh FI, Owais AI, Kawaja W. Ability of pit and fissure sealant-containing amorphous calcium phosphate to inhibit enamel demineralization. Int J Clin Pediatr Dent 2016;9(1):10-14.

Bedsharing and Oral Health-related Feeding Behaviors Among Zero- to Three-year-old Children.

PURPOSE: The purpose of this study was to investigate the association between bedsharing and oral health-related feeding behaviors among zero- to three-year-old children. METHODS: Data were collected from records of children attending the University of Iowa's Infant Oral Health Program (1998 to 2009), including maternal demographics, children's nighttime dietary practices, bedsharing habits, methods to help children sleep, and clinical evidence of plaque and caries. Statistical analyses utilized bivariate and logistic regression analyses. RESULTS: Mother-child dyads (n equals 629) who either bedshared every night (n equals 273) or did not bedshare (n equals 356) were included. Of the 629 children (mean±standard deviation [SD] age equals 21.6±12.1 months), 51 percent were male, 50 percent were nonwhite, and 72 percent lived with both parents. Logistic regression analysis revealed that children who bedshared were more likely to be healthy (OR equals 6.78; P=.008), older (OR equals 1.56; P=.007), nonwhite (OR equals 2.96; P<.001), live with a single mother (OR equals 3.41; P<.001), breastfed throughout the night (OR equals 4.33; P<.001), and bottle-fed and breastfed to help them sleep (OR equals 2.34; P=.03; OR equals 3.27; P=.03, respectively). Moreover, these children were more likely to be in the high caries-risk category (OR equals 2.19; P=.01). CONCLUSIONS: Bedsharing was significantly associated with the child's health status, age, race, nighttime feeding habits, and high caries-risk and with whom the child lived. Caries preventive measures should be targeted to mother-child dyads who bedshare.

Dental age estimation of Jordanian children: applicability of Demirjian method / Estimativa de idade dentária em crianças jordanas: aplicabilidade do método Demirjian

ABSTRACT Objectives: To evaluate the accuracy of Demirjian method in estimating the chronological age of male and female Jordanian children and to establish a new dental age curve if the Demirjian method was not found to be accurate. Methods: Orthopantomograms (OPTs) of 1374 Caucasian Jordanian children (684 females and 690 males) aged 4 to16 years were selected and the dental age was determined by Demirjian method. The chronological ages of the children were obtained by subtracting their birthdates from the date of taking the radiograph. The OPTs were obtained from Archives of Dental Teaching Clinics /XXX and other private orthodontic practices in Irbid and Amman. Results: Demirjian method overestimated chronological age in female and male subjects aged 4 to 8 years. Afterwards, the method underestimated chronological age in females aged 9-11 years and 14-16 years. In male subjects, chronological age was underestimated in subjects aged 9-12 years and 15-16 years. New the dental age curves for Jordanian females and males were constructed. The constants for the quadratic model for the new curves were (b0=-25.341, b1=17.557, b2=-0.623) for females and (b0=-29.809, b1=17.396, b2=-0.595) for males. Conclusion: Demirjian method overestimated the chronological age of Jordanians below the age of 8 years and underestimated the age of Jordanians above 8 years. A new DA standard for Jordanian children was developed and tested for accuracy.

RESUMO Objetivo: Avaliar a precisão do método Demirjian na estimativa da idade cronológica de crianças jordanianas do sexo masculino e feminino e estabelecer uma nova curva de idade dentária, se o método Demirjian não for acurado. Métodos: Foram selecionados radiografias panorâmicas (OPTs) de 1374 crianças jordanianas caucasianas (684 do sexo feminino e 690 do sexo masculino) com idades entre 4 e 16 anos, e a idade dentária foi determinada pelo método de Demirjian. As idades cronológicas das crianças foram obtidas subtraindo as datas de nascimento a partir da data da realização da radiografia. Os OPTs foram obtidos em Archives of Dental Teaching Clinics / XXX e outras clínicas ortodônticas particulares em Irbid e Amã. Resultados: O método Demirjian superestimou a idade cronológica em indivíduos do sexo feminino e masculino com idades entre 4 e 8 anos. Posteriormente, o método subestimou a idade cronológica em mulheres de 9 a 11 anos e 14 a 16 anos. Nos homens, a idade cronológica foi subestimada nos indivíduos de 9 a 12 anos e 15 a 16 anos. Novas curvas de idade dentária para mulheres e homens jordanianos foram construídas. As constantes para o modelo quadrático para as novas curvas foram (b0 = -25.341, b1 = 17.557, b2 = -0.623) para mulheres e (b0 = -29.809, b1 = 17.396, b2 = -0.595) para homens. Conclusão O método Demirjian superestimou a idade cronológica dos jordanianos abaixo de 8 anos e subestimou a idade dos jordanianos acima de 8 anos. Um novo padrão DA para crianças jordanianas foi desenvolvido e testado quanto à precisão.