Facile Fabrication of Multifunctional Hydrogel Nanoweb Coating Using Carboxymethyl Chitosan-Based Short Nanofibers for Blood-Contacting Medical Devices.

Blood-contacting medical devices (BCDs) require antithrombotic, antibacterial, and low-friction surfaces. Incorporating a nanostructured surface with the functional hydrogel onto BCD surfaces can enhance the performances; however, their fabrication remains challenging. Here, we introduce a straightforward method to fabricate a multifunctional hydrogel-based nanostructure on BCD surfaces using O-carboxymethyl chitosan-based short nanofibers (CMC-SNFs). CMC-SNFs, fabricated via electrospinning and cutting processes, are easily sprayed and entangled onto the BCD surface. The deposited CMC-SNFs form a robust nanoweb layer via fusion at the contact area of the nanofiber interfaces. The superhydrophilic CMS-SNF nanoweb surface creates a water-bound layer that effectively prevents the nonspecific adhesion of bacteria and blood cells, thereby enhancing both antimicrobial and antithrombotic performances. Furthermore, the CMC-SNF nanoweb exhibits excellent lubricity and durability on the bovine aorta. The demonstration results of the CMC-SNF coating on catheters and sheaths provide evidence of its capability to apply multifunctional surfaces simply for diverse BCDs.

Performance comparison of photodynamic antimicrobial chemotherapy with visible-light-activated organic dyes: Rose bengal, crystal violet, methylene blue, and toluidine blue O.

This study evaluated the photobiocidal performance of four widely distributed visible-light-activated (VLA) dyes against two bacteria (Staphylococcus epidermidis and Escherichia coli) and two bacteriophages (phages MS2 and phi 6): rose bengal (RB), crystal violet, methylene blue, and toluidine blue O (TBO). The photobiocidal performance of each dye depended on the relationship between the type of dye and microorganism. Gram-negative E. coli and the non-enveloped structure of phage MS2 showed more resistance to the photobiocidal reaction than Gram-positive S. epidermidis and the enveloped structure of phage phi 6. RB had the highest potential to yield reactive oxygen species. However, the photobiocidal performance of RB was dependent on the magnitude of the surface charge of the microorganisms; for example, anionic RB induced a negative surface charge and thus electrical repulsion. On the other hand, the photobiocidal performance of TBO was observed to be less affected by the microorganism type. The comparative results presented in our study have significant implications for selecting photodynamic antimicrobial chemotherapy (PACT) dyes suitable for specific situations and purposes. Furthermore, they contribute to the advancement of PACT-related technologies by enhancing their applicability and scalability.

Enhanced Scanning Electron Microscopy Using Auto-Optimized Image Restoration With Constrained Least Squares Filter for Nanoscience.

The growing demands of nanoscience require the continuous improvement of visualization methods. The imaging performance of scanning electron microscopy (SEM) is fundamentally limited by the point spread function of the electron beam and degrades because of noise. This paper proposes an auto-optimization algorithm based on deconvolution for the restoration of SEM images. This algorithm uses a constrained least squares filter and does not dependent on the user's experience or the availability of nondegraded images. The proposed algorithm improved the quality of the SEM images of 10-nm Au nanoparticles, and achieved balance among the sharpness, contrast-to-noise ratio (CNR), and image artifacts. For the SEM image of 100-nm pitched line patterns, the analysis of the spatial frequencies allowed the 2.5-fold improvement of the intensity of 4-nm information, and the noise floor decreased approximately 32 times. Along with the results obtained by the application of the proposed algorithm to images of tungsten disulfide (WS2) flakes, carbon nanotubes (CNTs), and HeLa cells, the evaluation results confirm that the proposed algorithm can enhance the SEM imaging of nanoscale features that lie close to the microscope's resolution limit.

Exploring the Potential of a Thermionic LaB6 Virtual Source Mode Electron Gun for a High Angular Current Density and a Narrow Energy Distribution.

To date, lanthanum hexaboride (LaB6) thermionic electron sources have not been able fully to capitalize on their inherent potential, resulting in an ambiguous position within the application area. Although they exhibit higher brightness compared with a tungsten filament source, they still fall short of the performance of Schottky electron sources. This study aims to explore the capabilities of the LaB6 electron source under different operating conditions to bridge the gap, ultimately to realize its untapped potential. Simulations in virtual source mode indicated enhanced beam brightness and a reduced beam half-angle with an increase the extraction voltage, promising up to tenfold times higher beam brightness compared with the crossover mode. The energy distribution measured using a prelens retarding field energy analyzer revealed an energy distribution of 0.55 eV and a high angular current density of 33 mA/sr in the virtual source mode. Therefore, the virtual source mode of LaB6 can provide a narrow energy distribution akin to that of a ZrO/W Schottky electron gun (1600 K) while having an angular current density over 2,000 times higher. In addition, the stability of the virtual source mode is ±0.022%, while that of the crossover mode is ±0.138%.

Prediction of indoor PM2.5 concentrations and reduction strategies for cooking events through various IAQ management methods in an apartment of South Korea.

Indoor PM2.5 in apartments must be effectively managed to minimize adverse impacts on human health. Cooking is the one of the main PM2.5 sources in apartments, and indoor air quality (IAQ) management methods (natural ventilation, mechanical ventilations, range hoods, and air purifiers) are typically used to reduce PM2.5 generated during cooking. For effective control of indoor PM2.5 , prediction of PM2.5 reduction for various IAQ management methods is necessary. This study carefully predicted indoor PM2.5 concentrations in an apartment when IAQ management methods were applied separately and/or in combination during cooking. The infiltration and exfiltration were verified by comparing the experimental results of CO2 concentration with those predicted with or without mechanical ventilation. The deposition rate for PM2.5 generated by cooking was also derived by comparing the experimental PM2.5 changes with the predicted values for PM2.5 natural decay. Through this method, effective PM2.5 control ways during cooking in apartments can be proposed, such as natural ventilation with a range hood for 30 min and then the operation of an air purifier for 30 min. Additionally, if this prediction is combined with energy consumption, it will be possible to propose the most energy-efficient indoor PM2.5 control methods for various seasons and outdoor conditions.

Photobiocidal-triboelectric nanolayer coating of photosensitizer/silica-alumina for reusable and visible-light-driven antibacterial/antiviral air filters.

Outbreaks of airborne pathogens pose a major threat to public health. Here we present a single-step nanocoating process to endow commercial face mask filters with photobiocidal activity, triboelectric filtration capability, and washability. These functions were successfully achieved with a composite nanolayer of silica-alumina (Si-Al) sol-gel, crystal violet (CV) photosensitizer, and hydrophobic electronegative molecules of 1H, 1H, 2H, 2H-perfluorooctyltriethoxysilane (PFOTES). The transparent Si-Al matrix strongly immobilized the photosensitizer molecules while dispersing them spatially, thus suppressing self-quenching. During nanolayer formation, PFOTES was anisotropically rearranged on the Si-Al matrix, promoting moisture resistance and triboelectric charging of the Si-Al/PFOTES-CV (SAPC)-coated filter. The SAPC nanolayer stabilized the photoexcited state of the photosensitizer and promoted redox reaction. Compared to pure-photosensitizer-coated filters, the SAPC filter showed substantially higher photobiocidal efficiency (∼99.99 % for bacteria and a virus) and photodurability (∼83 % reduction in bactericidal efficiency for the pure-photosensitizer filter but ∼0.34 % for the SAPC filter after 72 h of light irradiation). Moreover, after five washes with detergent, the SAPC filter maintained its photobiocidal and filtration performance, proving its reusability potential. Therefore, this SAPC nanolayer coating provides a practical strategy for manufacturing an antimicrobial and reusable mask filter for use during the ongoing COVID-19 pandemic.

A Novel Monochromator with Offset Cylindrical Lenses and Its Application to a Low-Voltage Scanning Electron Microscope.

Low-voltage scanning electron microscopes (LV-SEMs) are widely used in nanoscience. However, image resolution for SEMs is restricted by chromatic aberration due to energy spread of the electron beam at low acceleration voltage. This study introduces a new monochromator (MC) with offset cylindrical lenses (CLs) as one solution for LV-SEMs. The MC optics, with highly excited CLs in offset layouts, has advantageous high performance and simple experimental setup, making it suitable for field emission LV-SEMs. In a preliminary evaluation, our MC reduced the energy spread from 770 to 67 meV. The MC was integrated into a commercial SEM equipped with an out-lens (a conventional objective lens without immersion magnetic or retarding electric fields) and an Everhart­Thornley detector. Comparing SEM images under two conditions with the MC turned on or off, the spatial resolution was improved by 58% at 0.5 and 1 keV. The filtering effect of the MC decreased the probe current with a ratio (i.e., transmittance) of 5.7%, which was consistent with estimations based on measured energy spreads. To the best of our knowledge, this is the first report on an effective MC with higher-energy resolution than 100 meV and the results offer encouraging prospects for LV-SEM technology.

High-Performance Compact Pre-Lens Retarding Field Energy Analyzer for Energy Distribution Measurements of an Electron Gun.

The energy distribution of an electron gun is one of the most important characteristics determining the performance of electron beam-based instruments, such as electron microscopes and electron energy loss spectroscopes. For accurate measurements of the energy distribution, this study presents a novel retarding field energy analyzer (RFEA) with the feature of an additional integrated pre-lens, which enables an adjustment of beam trajectory into the analyzer. The advantages of this analyzer are its compact size and simple electrode configuration. According to trajectory simulation theories, the optimum condition arises when the incident electron beam inside the RFEA is focused on the center of a retarding electrode. Comparing I­V curves depending on whether the pre-lens working or not, it is confirmed that the use of the pre-lens dramatically improves the energy resolution and efficiency of the signal acquisition process. The pre-lens RFEA was applied to characterize a Schottky electron gun under various temperatures and extraction voltages as operational conditions. When the tip temperature was increased by 50 K, we were able to measure an energy distribution broadening of 13.8 meV with the proposed pre-lens RFEA. The relative standard deviation of energy distribution was 0.7% for each working condition.

Serial number coding and decoding by laser interference direct patterning on the original product surface for anti-counterfeiting.

Here, we investigate a method to distinguish the counterfeits by patterning multiple reflective type grating directly on the surface of the original product and analyze the serial number from its rotation angles of diffracted fringes. The micro-sized gratings were fabricated on the surface of the material at high speeds by illuminating the interference fringe generated by passing a high-energy pulse laser through the Fresnel biprism. In addition, analysis of the grating's diffraction fringes was performed using a continuous wave laser.

Observation of strongly enhanced ultrashort pulses in 3-D metallic funnel-waveguide.

For strong field enhancement of ultrashort light pulses, a 3-D metallic funnel-waveguide is analyzed using the finite-difference time-domain (FDTD) method. Then the maximum intensity enhancement actually developed by the funnel-waveguide upon the injection of femtosecond laser pulses is observed using two-photon luminescence (TPL) microscopy. In addition, the ultrafast dephasing profile of the localized field at the hot spot of the funnel-waveguide is verified through the interferometric autocorrelation of the TPL signal. Finally it is concluded the funnel-waveguide is an effective 3-D nanostructure that is capable of boosting the peak pulse intensity of stronger than 80 TWcm(-2) by an enhancement factor of 20 dB without significant degradation of the ultrafast spatiotemporal characteristics of the original pulses.

High-harmonic generation by resonant plasmon field enhancement.

High-harmonic generation by focusing a femtosecond laser onto a gas is a well-known method of producing coherent extreme-ultraviolet (EUV) light. This nonlinear conversion process requires high pulse intensities, greater than 10(13) W cm(-2), which are not directly attainable using only the output power of a femtosecond oscillator. Chirped-pulse amplification enables the pulse intensity to exceed this threshold by incorporating several regenerative and/or multi-pass amplifier cavities in tandem. Intracavity pulse amplification (designed not to reduce the pulse repetition rate) also requires a long cavity. Here we demonstrate a method of high-harmonic generation that requires no extra cavities. This is achieved by exploiting the local field enhancement induced by resonant plasmons within a metallic nanostructure consisting of bow-tie-shaped gold elements on a sapphire substrate. In our experiment, the output beam emitted from a modest femtosecond oscillator (100-kW peak power, 1.3-nJ pulse energy and 10-fs pulse duration) is directly focused onto the nanostructure with a pulse intensity of only 10(11) W cm(-2). The enhancement factor exceeds 20 dB, which is sufficient to produce EUV wavelengths down to 47 nm by injection with an argon gas jet. The method could form the basis for constructing laptop-sized EUV light sources for advanced lithography and high-resolution imaging applications.

Design and optimization of a conical electrostatic objective lens of a low-voltage scanning electron microscope for surface imaging and analysis in ultra-high-vacuum environment.

Low-voltage scanning electron microscopy (LV-SEM) with landing energies below 5 keV has been widely used due to its advantages in mitigating the damage and charging effects to a specimen and enhancing surface information due to small interaction volume of electrons inside a specimen. Additionally, for elemental analysis of the surfaces of bulk specimens with Auger electron spectroscopy (AES) or electron energy loss spectroscopy (EELS), ultra-high-vacuum (UHV) environment is essential to maintain clean surfaces without the absorption of gas molecules during the electron beam irradiation for the acquisition of spectral data. In this study, we propose the optimal design and condition of a conical Electrostatic Objective Lens (EOL) for a UHV LV-SEM to achieve the high spatial resolution and secondary electron (SE) detection efficiency. The EOL is composed of only the three electrodes (retarding, focusing and booster electrodes) and the insulators, which is suitable for maintaining a UHV environment with less out-gassing. The cone angle of the EOL is determined as 60° to integrate a spectrometer in the UHV LV-SEM and in a large size and a higher tilt angle of the sample. Through the optimization with the simulations, the EOL achieves the minimized spherical and chromatic aberration coefficients of 0.05 and 0.03 mm at the sample side, respectively, at the landing energy of 50 eV and the shortest working distance (WD) of 1 mm for high-resolution imaging. In addition, the probe diameter of the optimized EOL is 2.3 nm at 1 keV and 5.7 nm at 50 eV with a WD of 1 mm and a probe current of 10 pA, which are comparable to previously studied compound objective lenses with magnetic and electrostatic lenses. Using a longer WD of 4 mm for analysis, the probe diameter was 5.4 nm at 1 keV and the SE detection efficiency was 83.3 % owing to the separated scintillator detector structure from the booster electrode. These results imply that the optimized EOL has the potential to be applied to a high-performance UHV LV-SEM for the surface imaging and analysis with a simple system configuration.

The Effectiveness of a Mechanical Ventilation System for Indoor PM2.5 in Residential Houses.

The mechanical ventilation systems used in houses are designed to reduce carbon dioxide emissions while minimizing the energy loss resulting from ventilation. However, the increase in indoor fine particulate (PM2.5) concentration because of external PM2.5 influx through the ventilation system poses a problem. Here, we analyzed the changes in indoor PM2.5 concentration, distinguishing between cases of high and low outdoor PM2.5 concentrations and considering the efficiency of the filters used in residential mechanical ventilation systems. When using filters with the minimum efficiency reporting value (MERV) of 10 in the ventilation system, the outdoor PM2.5 concentration was 5 µg/m³; compared to the initial concentration, the indoor PM2.5 concentration after 60 min decreased to 73%. When the outdoor PM2.5 concentration was 30-40 µg/m³, the indoor PM2.5 concentration reached 91%. However, when MERV 13 filters were used, the indoor PM2.5 concentration consistently dropped to 73-76%, regardless of the outdoor PM2.5 concentration. Furthermore, by comparing the established equation with the mass balance model, the error was confirmed to be within 5%, indicating a good fit. This allows for the prediction of indoor PM2.5 under various conditions when using mechanical ventilation systems, enabling the formulation of strategies for maintaining indoor PM2.5, as recommended by the World Health Organization.

Quartz crystal microbalance with thermally-controlled surface adhesion for an efficient fine dust collection and sensing.

The mass concentration of fine dust or particles acts as a standard measure to express the severity of air pollution. In connection with this, many related sensor technologies have been suggested for both indoor and outdoor uses. Among several technologies, the direct measurement of the dust mass using resonant platforms is the most preferable as it possesses multiple advantages including high sensitivity, low limit of detection, and a rapid response time. Such sensor performances directly rely on the adhesion quality between the sensor substrate and dust. In this work, we introduce a thermally controlled dust capturing scheme by integrating a polystyrene (PS) layer and microheater on quartz crystal microbalance (QCM). The Pt microheater can rapidly heat the sensor up to 100 °C, allowing a controlled switching between the soft and hard conditions of the PS film at a rapid rate. When the film is soft, the sensor can capture dust particle efficiently and we can calibrate the attached particle mass by measuring the resonance response. Compared to a bare QCM, our sensor used in this study exhibits 11 times larger detectable mass range. In addition, heated QCMs show a performance that is comparable to a high-cost particle sensing equipment such as an aerodynamic particle sizer and optical particle counter.

Multiplexed electrospraying of water in cone-jet mode using a UV-embossed pyramidal micronozzle film.

The electrospraying of water in the cone-jet mode is difficult in practical applications owing to its low throughput and the electrical discharge caused by the high surface tension of water. A film with multiple dielectric micronozzles is essential for multiplexed electrospraying of water in cone-jet mode without electrical discharge. Thus, a pyramidal micronozzle film with five nozzles was fabricated using the UV-embossing process. The pyramidal micronozzle film consisted of pyramidal micronozzles, a micropillar array, and an in-plane extractor, which were proposed to minimize wetting and concentrate the electric field to the water meniscus at the tip of the pyramidal micronozzle. The electrospraying of water using a single pyramidal micronozzle was visualized by a high-speed camera at a flow rate of 0.15-0.50 ml/h with voltages of 0.0-2.3 kV, -1.6 kV, and -4.0 kV at the water, guide ring, and collector, respectively. Three distinct modes, the dripping, spindle, and cone-jet modes, were observed and distinguished according to the motion of the water meniscus at the nozzle tip. The steady Taylor cone and jet were observed in a voltage range of 1.3-2.0 kV in water, particularly in cone-jet mode. Multiplexed electrospraying of water in cone-jet mode at a flow rate of 1.5 ml/h was performed using a pyramidal micronozzle film, demonstrating the potential for a high-throughput electrospraying system.

The Actual Efficacy of an Air Purifier at Different Outdoor PM2.5 Concentrations in Residential Houses with Different Airtightness.

It is important to control airborne particles in residential houses for protecting human health. Indoor particulate matter of <2.5 µm (PM2.5) can be effectively monitored and managed using an air purifier. In this study, the actual clean air delivery rates in residential houses (CADRActual) were acquired by comparing decay rates of fine particles with and without operations of the air purifier under actual conditions, following the standard CADR of an air purifier obtained in a closed test chamber. The measurements of CADRActual at different outdoor PM2.5 concentrations over a month in two residential houses revealed different airtightness levels, compared to the standardized clean air delivery rate of the air purifier (CADRAP). Air changes per hour at 50 Pa (ACH50) was 4.8 h−1 for "house A" (built in 2007) and 2.1 h−1 for "house B" (built in 2018). The CADR of the air purifier used in this study was 10.6 m3/min, while the averaged CADRActual at the "house A" was 7.2 m3/min (approximately 66% of the CADR of the air purifier) and 9.5 m3/min at "house B" (approximately 90% of the CADR of the air purifier). Under the outdoor PM2.5 concentrations of <35 µg/m3, the averaged CADRActual of house A and house B were 7.8 ± 0.3 and 9.7 ± 0.4 m3/min, respectively. However, under the outdoor PM2.5 concentrations of >35 µg/m3, the analogous averaged concentrations were 6.8 ± 0.6 and 9.6 ± 0.3 m3/min for houses A and B, respectively. The measured CADRActual agreed well with the theoretical estimates of CADRActual acquired by the mass balance equation using the infiltration rate of ACH50/20. We also estimated CADRActual/CADRAP for house C built in 2017, where the ACH50 was 1.8 h−1. Overall, this study demonstrated how CADRActual/CADRAP of an air purifier at residential houses can be predicted according to outdoor PM2.5 concentration and airtightness of the house. As shown, it can be closer to 1 at lower ACH50 houses and at lower outdoor PM2.5 concentrations.

Efficient Energy Saving Scenarios for Indoor PM2.5 Management in an Apartment of South Korea.

Indoor PM2.5 must be effectively controlled to minimize adverse impacts on public health. Cooking is one of the main sources of PM2.5 in residential areas, and indoor air quality (IAQ) management methods such as natural and mechanical ventilation, range hood, and air purifier are typically used to reduce cooking-generated PM2.5 concentrations. However, studies on the combined effects of various IAQ management methods on indoor PM2.5 reduction and energy consumption are limited. In this study, a theoretical model was established to estimate the performance of various IAQ management methods for controlling indoor PM2.5 concentrations and energy consumption. The model was verified by comparative experiments in which, various IAQ management methods were operated individually or combined. Seasonal energy consumption was calculated through the verified model, and energy consumption saving scenarios were derived for maintaining indoor PM2.5 concentrations less than 10 µg/m3, a World Health Organization annual guideline, under fair and poor outdoor PM2.5 concentrations of 15 and 50 µg/m3, respectively. Based on our results, we found that energy consumption could be reduced significantly by applying natural ventilation in spring, autumn, and summer and mechanical ventilation in winter. Our study identified efficient energy saving PM2.5 management scenarios using various IAQ management methods by predicting indoor PM2.5 concentration and energy consumption according to the annual life patterns of typical residents in South Korea.

Effects of Air Purifiers on the Spread of Simulated Respiratory Droplet Nuclei and Virus Aggregates.

The present study was performed to quantitatively evaluate the effects of air purifiers on the spread of COVID-19 and to suggest guidelines for their safe use. To simulate respiratory droplet nuclei and nano-sized virus aggregates, deionized water containing 100 nm of polystyrene latex (PSL) particles was sprayed using a vibrating mesh nebulizer, and the changes in the particle number concentration were measured for various locations of the particle source and air purifier in a standard 30 m3 test chamber. The spread of the simulated respiratory droplet nuclei by the air purifier was not significant, but the nano-sized aggregates were significantly affected by the airflow generated by the air purifier. However, due to the removal of the airborne particles by the HEPA filter contained in the air purifier, continuous operation of the air purifier reduced the number concentration of both the simulated respiratory droplet nuclei and nano-sized aggregates in comparison to the experiment without operation of the air purifier. The effect of the airflow generated by the air purifier on the spread of simulated respiratory droplet nuclei and nano-sized aggregates was negligible when the distance between the air purifier and the nebulizer exceeded 1 m.

High-sensitivity microvolume UV absorption spectrometry for routine analysis of small-volume biological samples.

Substantial improvement of microvolume UV absorption spectrometry in sensitivity, robustness and ease of operation was achieved for routine biological applications. A unique microtubing-based absorption cell (208 µm internal diameter) featuring enhanced light transmission with a liquid core waveguide technique provided dramatically enhanced absorption sensitivity, proportional to the extended path length (50 mm, from the typical 1 mm), while robust measurement performance was attained by implementation of preventive measures against bubble trapping along the light path. For pBR322 plasmid DNA, absorbance at 260 nm was reliably measurable down to 0.1 ng/µl with repeatability typically 2-3% RSD. The detection limit was 0.03 ng/µl dsDNA, far lower than the current state-of-the-art âˆ¼1 ng/µl. Sample consumption for each measurement was 2.4 µl. Automated operation implemented for the first time in microvolume spectrophotometry facilitated the ease in handling with small-volume biological samples.