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Superparamagnetic nanoparticles are being actively developed for various applications in oil fields. A key requirement for such nanoparticles is the long-term stability of their dispersion under harsh subsurface conditions such as high salinity and high temperature. In this study, through functionalization of the surface of the magnetite nanoparticles with a sulfonated phenolic resin, stable dispersions of the nanoparticles in 5 wt % NaCl solution at neutral to basic pH were achieved. Sulfonated phenolic resin is aromatic in nature and can endure high temperatures; moreover, the strongly anionic sulfonate groups facilitate sufficient electrostatic repulsion between the particles even in a highly saline environment. Sulfonated phenolic resin was synthesized by heating a mixture of 4-hydroxybenzenesulfonic acid and formaldehyde at 1:1 molar ratio. Then, by synthesizing magnetite nanoparticles by a co-precipitation process in a solution of sulfonated phenolic resin, one-pot synthesis/surface functionalization was accomplished, which not only simplifies the manufacturing process but also can potentially lower the cost of production owing to the use of readily available phenolic resins. The reliability of the technique was confirmed by the detailed characterization of the nanoparticle through size distribution analysis, thermogravimetric analysis, ζ-potential measurements, transmission electron microscopy imaging, and energy-dispersive X-ray spectroscopy mapping. Further, Langevin curve was generated for evaluating the superparamagnetism of the nanoparticles.
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The high linear correlation between the smartphone magnetometer readings in close proximity can be exploited for physical human contact detection, which could be useful for such applications as infectious disease contact tracing or social behavior monitoring. Alternative approaches using other capabilities in smartphones have aspects that do not fit well with the human contact detection. Using Wi-Fi or cellular fingerprints have larger localization errors than close human contact distances. Bluetooth beacons could reveal the identity of the transmitter, threatening the privacy of the user. Also, using sensors such as GPS does not work for indoor contacts. However, the magnetometer correlation check works best in human contact distances that matter in infectious disease transmissions or social interactions. The omni-present geomagnetism makes it work both indoors and outdoors, and the measured magnetometer values do not easily reveal the identity and the location of the smartphone. One issue with the magnetometer-based contact detection, however, is the energy consumption. Since the contacts can take place anytime, the magnetometer sensing and recording should be running continuously. Therefore, how we address the energy requirement for the extended and continuous operation can decide the viability of the whole idea. However, then, we note that almost all existing magnetometer-based applications such as indoor location and navigation have used high sensing frequencies, ranging from 10 Hz to 200 Hz. At these frequencies, we measure that the time to complete battery drain in a typical smartphone is shortened by three to twelve hours. The heavy toll raises the question as to whether the magnetometer-based contact detection can avoid such high sensing rates while not losing the contact detection accuracy. In order to answer the question, we conduct a measurement-based study using independently produced magnetometer traces from three different countries. Specifically, we gradually remove high frequency components in the traces, while observing the correlation changes. As a result, we find that the human coexistence detection indeed tends to be no less, if not more, effective at the sampling frequency of 1 Hz or even less. This is because unlike the other applications that require centimeter-level precision, the human contacts detected anywhere within a couple of meters are valid for our purpose. With the typical smartphone battery capacity and at the 1 Hz sensing, the battery consumption is well below an hour, which is smaller by more than two hours compared with 10 Hz sampling and by almost eleven hours compared with 200 Hz sampling. With other tasks running simultaneously on smartphones, the energy saving aspect will only become more critical. Therefore, we conclude that sensing the ambient magnetic field at 1 Hz is sufficient for the human contact monitoring purpose. We expect that this finding will have a significant practicability implication in the smartphone magnetometer-based contact monitoring applications in general.
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Teléfono Inteligente , Comunicación , HumanosRESUMEN
Existing works in photovoltaic (PV) power generation focus on accurately predicting the PV power output on a forecast horizon. As the solar power generation is heavily influenced by meteorological conditions such as solar radiation, the weather forecast is a critical input in the prediction performance. However, the weather forecast is traditionally considered to have coarse granularity, so many are compelled to use on-site meteorological sensors to complement it. However, the approach involving on-site sensors has several issues. First, it incurs the cost in the installation, operation, and management of the sensors. Second, the physical model of the sensor dynamics itself can be a source of forecast errors. Third, it requires an accumulation of sensory data that represent all seasonal variations, which takes time to collect. In this paper, we take an alternative approach to use a relatively large deep neural network (DNN) instead of the on-site sensors to cope with the coarse-grained weather forecast. With historical PV output power data from our grid-connected building with a rooftop PV power generation facility and the publicly available weather forecast history data, we demonstrate that we can train a six-layer feedforward DNN for the day-ahead forecast. It achieves the average mean absolute error (MAE) of 2.9%, comparable to that of the conventional model, but without involing the on-site sensors.
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Orai1 is a pore-subunit of store-operated Ca(2+) release-activated Ca(2+) (CRAC) channel that mediates Ca(2+) influx in most non-excitable cells via store-operated Ca(2+) entry (SOCE) mechanism. We previously demonstrated that Orai1 is involved in mediating osteogenic potential of mesenchymal stem cells (MSCs), but the underlying mechanism of this function remains unknown. Here, we report that Orai1 mediates osteogenic differentiation via bone morphogenic protein (BMP) signaling pathway in bone marrow MSCs (BMSCs). In osteogenic conditions, BMSCs derived from wild-type mice underwent osteoblastic differentiation and induced mineralization as demonstrated by increased alkaline phosphatase activity and alizarin red S staining, respectively. The expression of Runx2, a master regulator of osteoblast differentiation, and osteogenic differentiation markers were markedly increased in wild-type BMSCs under osteogenic conditions. In contrast, osteogenic conditions failed to induce such effects in BMSCs derived from Orai1-deficient (Orai1(-/-)) mice, indicating that Orai1 is, in part, necessary for osteogenic differentiation of MSCs. We also found that BMP2 successfully induced phosphorylation of Smad1/5/8, the immediate effector molecules of BMP signaling, in wild-type BMSCs, but failed to do so in Orai1(-/-) BMSCs. Downstream target genes of BMP signaling pathway were consistently increased by osteogenic conditions in wild-type BMSCs, but not in Orai1(-/-) BMSCs, suggesting a novel molecular link between Orai1 and BMP signaling pathway in the osteogenic differentiation process. Further functional studies demonstrated that activation of BMP signaling rescues osteogenic differentiation capacity of Orai1(-/-) BMSCs. In conclusion, Orai1 regulates osteogenic differentiation through BMP signaling, and the Orai1-BMP signaling may be a possible therapeutic target for treating bone-related diseases.
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Proteínas Morfogenéticas Óseas/metabolismo , Calcificación Fisiológica/fisiología , Células Madre Mesenquimatosas/citología , Células Madre Mesenquimatosas/fisiología , Osteogénesis/fisiología , Transducción de Señal/fisiología , Animales , Células de la Médula Ósea/citología , Células de la Médula Ósea/fisiología , Señalización del Calcio , Diferenciación Celular/fisiología , Células Cultivadas , RatonesRESUMEN
OBJECTIVE: The aim of this study was to compare new bone formation with titanium (Ti) surface and hydroxyapatite (HA)-coated titanium surface in mucosal perforation model. MATERIALS AND METHODS: HA coating to the Ti disc and implant were done by aerosol deposition technique. Alkaline phosphatase assay and cell migration assay were done in Ti and HA surface disc with MG63 cells. For the in vivo test, 5 New Zealand white rabbits were used. Two penetration defects were prepared in the nasal bone. Subsequently, 2 types of implants were installed into the defect (diameter: 3.0 mm, length: 6.0 mm). Approximately 5.0 mm of the fixture's surface penetrated into the nasal cavity. In the experimental group, HA-coated implants were used. The same design of implants without coating was used in the control group. The animals were sacrificed 8 weeks postoperatively. Subsequently, a histomorphometric analysis was done. RESULTS: Alkaline phosphatase activity was significantly higher in HA-coated surface than in titanium surface (P < 0.05). In addition, more cells were migrated into the HA-coated surface when compared to Ti surface. In the animal experiments, mean new bone formation was 30.68 ± 14.16% in the experimental group and 6.92 ± 5.12% in the control group (P = 0.001). Mean bone-to-implant contact was 31.71 ± 8.41% in the experimental group and 7.98 ± 5.58% in the control group (P < 0.001). Mean height of the bone regeneration was 3.70 ± 0.76 mm in the experimental group and 1.04 ± 0.67 mm in the control group. The difference between the 2 groups was statistically significant (P < 0.001). CONCLUSIONS: HA-coated implants exhibited more bone regeneration in the mucosal penetration model than the uncoated implants.
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Durapatita/farmacología , Hueso Nasal/cirugía , Osteogénesis/efectos de los fármacos , Prótesis e Implantes , Titanio/farmacología , Aerosoles , Fosfatasa Alcalina/análisis , Animales , Movimiento Celular/efectos de los fármacos , Materiales Biocompatibles Revestidos , Microscopía Electrónica de Rastreo , Hueso Nasal/enzimología , ConejosRESUMEN
PURPOSE: The aim of this study was to assess quantitatively whether a symmetric reconstruction of the calvaria could be achieved using 3-dimensional (3D) custom-made implants and to examine any complications caused by the cranioplasty. PATIENTS AND METHODS: Custom-made cranial implants were produced using data obtained from computed tomographic scanning of the defect using computer-aided design and rapid prototyping techniques. Polymethylmethacrylate was used as the reconstruction material and the implant was cast from a silicone rubber mold. These implants were used in 10 patients (9 men and 1 woman) who previously received a craniectomy. The symmetry gained after cranioplasty was quantified by volumetric analysis using 3D reconstructed postoperative computed tomographic imaging. Any complications after cranioplasty also were recorded. RESULTS: The average follow-up was 42.5 months (range, 7 to 85 mo). The esthetic appearance of all patients was much improved. When the volume of the reconstructed right calvaria was compared with the left calvaria, the difference was not statistically significant (P > .05). There were 2 cases of complications. One exhibited a transient seroma collection. Another had a wrinkle formation in the forehead. No infectious episodes or signs of plate rejection were encountered. CONCLUSIONS: The custom-made implants for cranioplasty showed a significant improvement in morphology. The implants may be very useful for repairing large and complex-shaped cranial defects. The technique may be useful for the bone reconstruction of other sites.
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Craneotomía/métodos , Procedimientos de Cirugía Plástica/métodos , Prótesis e Implantes , Adulto , Materiales Biocompatibles/química , Diseño Asistido por Computadora , Estética , Femenino , Estudios de Seguimiento , Frente/patología , Humanos , Imagenología Tridimensional/métodos , Masculino , Persona de Mediana Edad , Modelos Anatómicos , Polimetil Metacrilato/química , Complicaciones Posoperatorias , Diseño de Prótesis , Implantación de Prótesis/métodos , Seroma/etiología , Piel/patología , Cráneo/lesiones , Cráneo/cirugía , Neoplasias Craneales/cirugía , Accidente Cerebrovascular/cirugía , Tomografía Computarizada Espiral/métodos , Interfaz Usuario-Computador , Adulto JovenRESUMEN
The use of halogen-based materials has been regulated since toxic substances are released during combustion. In this study, polyurethane foam was coated with cationic starch (CS) and montmorillonite (MMT) nano-clay using a spray-assisted layer-by-layer (LbL) assembly to develop an eco-friendly, high-performance flame-retardant coating agent. The thickness of the CS/MMT coating layer was confirmed to have increased uniformly as the layers were stacked. Likewise, a cone calorimetry test confirmed that the heat release rate and total heat release of the coated foam decreased by about 1/2, and a flame test showed improved fire retardancy based on the analysis of combustion speed, flame size, and residues of the LbL-coated foam. More importantly, an additional cone calorimeter test was performed after conducting more than 1000 compressions to assess the durability of the flame-retardant coating layer when applied in real life, confirming the durability of the LbL coating by the lasting flame retardancy.
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INTRODUCTION: This report describes the treatment of an immature mandibular molar by combining vital pulp therapy (VPT) and regenerative endodontic procedures (REPs). It details the use of REP to regain functionality and continued root development of an immature root with pulp necrosis and VPT for an immature root containing vital pulpal tissues. METHODS: An 8-year old male presented for evaluation of a mandibular first right molar with mild buccal swelling and a nontraceable sinus tract. He recently had received a restoration. After intraoral and radiographic examination, a diagnosis of pulp necrosis and chronic apical abscess was made. After access, pulp necrosis was confirmed in the distal root; however, vital pulp tissues were present in the mesial canals. It was decided on pulpotomy (VPT) in the mesial and REP in the distal root. At the initial visit, pulpotomy was completed in the mesial root, and REP was initiated in the distal root. Three weeks later, the patient was asymptomatic and the sinus tract absent. REP was completed in the distal root, and the tooth was restored. RESULTS: At the 6-, 12-, and 18-month follow-up, the patient presented without symptoms, and the tooth responded positively to pulp sensibility tests. Radiographic examinations showed resolution of the apical radiolucency and completed root development. CONCLUSIONS: Combined treatment using both VPT and REP for immature molars with different pulpal status in individual roots may be a preferable treatment option because preservation of vital pulp tissues and regeneration of new vital tissues allow for continued root development and functionality.
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Diente Molar , Niño , Necrosis de la Pulpa Dental , Humanos , Masculino , Pulpotomía , Endodoncia Regenerativa , Raíz del DienteRESUMEN
Mobile services' rapid evolution and development has meant that their evaluation has become a more and more pressing issue, and from both the practical and theoretical standpoints. The significant previous work in the field of multiple-criteria decision-making based evaluation of mobile services has some practical limitations that should be noted. First, there has been insufficient research that has utilized both objective and subjective weighting. Second, the investigations that have employed Vise Kriterijumska Optimizacija I Kompromisno Resenje (VIKOR), a well known practical tool for use in multi-criteria decision making, did not consider the fuzzy environment. In order to fill these gaps in the literature, the present study developed fuzzy VIKOR for use with an integrated weighting approach that combines subjective and objective weighting to account for mobile services' various characteristics and, thereby, evaluate their quality. For subjective weighting, Decision Making Trial and Evaluation Laboratory (DEMATEL) was employed for simple determination of the weighting and causal relationships. For objective weighting of evaluation criteria, Shannon entropy was utilized. This study has a unique contribution in that it reflects the special circumstances of the mobile service evaluation that have not been considered in the previous studies. Especially, in this study, not only the subjective weighting method but also the objective weighting method are used for more accurate importance weight of evaluation criteria. In the novelty aspect, this is the first study trying to utilize fuzzy VIKOR in concert with a novel combined subjective/objective weighting method in order to integrate objective decision-matrix-derived information with subjective decision-maker preferences. Additionally, a supplemental, empirical mobile-service-evaluation case study was conducted that enables researchers and practitioners to better understand the overall, practical evaluation process. Validation of the case study results by comparison with other, representative multiple-criteria decision-making methods verified the proposed method's robustness.
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Algoritmos , Lógica Difusa , Tecnología Inalámbrica , Entropía , Lingüística , Reproducibilidad de los ResultadosRESUMEN
[This corrects the article DOI: 10.1371/journal.pone.0217786.].
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INTRODUCTION: Studies have shown that there is a significantly higher concentration of interleukin 6 (IL-6) in inflamed pulp tissues compared with healthy pulp tissues. The aims of this study were to investigate the baseline differences between mesenchymal stem cells (MSCs) isolated from healthy human dental pulp stem cells (H-DPSCs) and inflamed dental pulp stem cells (I-DPSCs) and their correlation to IL-6 and to determine whether IL-6 can affect the differentiation potentials of these cells. METHODS: MSCs isolated from healthy and inflamed pulp tissues were cultured and characterized in vitro. The levels of secreted IL-6 in the culture supernatants from H-DPSCs and I-DPSCs were measured by enzyme-linked immunosorbent assay. IL-6 and neutralizing IL-6 were added to H-DPSCs and I-DPSCs, respectively. Immunofluorescence staining, alizarin red staining, and Western blotting were performed to assess the differentiation potentials of H-DPSCs and I-DPSCs. The independent unpaired 2-tailed Student's t-test was performed after quantification analysis. RESULTS: H-DPSCs and I-DPSCs showed a similar expression of MSC-associated markers including CD44, CD73, CD90, and CD105, whereas H-DPSCs showed a lower level of IL-6, lower osteogenic differentiation potentials, and higher neurogenic differentiation potentials compared with I-DPSCs. The addition of IL-6 to H-DPSCs increased osteogenic potentials and decreased neurogenic potentials, whereas the neutralization of IL-6 for I-DPSCs led to decreased osteogenic potentials and increased neurogenic potentials. CONCLUSIONS: The findings of this study indicated IL-6 has the capacity to enhance osteogenesis while hindering neurogenesis of DPSCs.
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Diferenciación Celular , Pulpa Dental , Interleucina-6 , Neurogénesis , Osteogénesis , Células Madre , Proliferación Celular , Células Cultivadas , Humanos , Interleucina-6/fisiologíaRESUMEN
A series of guanylsulfonamides, 2-amino-9-[2-substituted-4-(4-substituted piperidin-1-sulfonyl)phenyl]-1,9-dihydropurin-6-ones, was synthesized by adopting reductive aminoformylation of 2-amino-5-nitro-6-[4-(piperidin-1-sulfonyl)phenylamino]-3H-pyrimidin- 4-one and subsequent intramolecular ring condensation as key steps. All the guanylsulfonamides were assayed for their in vitro antibacterial activities against Klebsiella pneumoniae, Escherichia coli, Pseudomonas aeruginosa, Bacillus subtilis, Staphylococcus aureus, and Streptococcus faecalis, and their antifungal activities against Aspergillus flavus, Aspergillus niger, and Candida albicans. Of the guanylsulfonamides, 13e and 13f displayed better antibacterial activities than that of Norfloxacin against the bacterial strains S. aureus and S. faecalis except 13f against S. faecalis, which exhibited the activity similar to that of Norfloxacin. Against the fungal strains A. flavus and A. niger, 13g and 13h showed similar activities to that of Griseoflavin-16 except 13h against A. niger, which displayed a profound drop in the activity compared to that of Griseoflavin-16. The remarkable inhibition of the growth of the bacterial and fungal strains makes these substances promising microbial agents.
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Antiinfecciosos/síntesis química , Antiinfecciosos/farmacología , Antifúngicos/síntesis química , Antifúngicos/farmacología , Sulfonamidas/síntesis química , Sulfonamidas/farmacología , Dimetilsulfóxido/farmacología , Evaluación Preclínica de Medicamentos/métodos , Enterobacter aerogenes/efectos de los fármacos , Enterobacter aerogenes/fisiología , Humanos , Pruebas de Sensibilidad Microbiana/métodos , Norfloxacino/farmacologíaAsunto(s)
Implantación Dental Endoósea/efectos adversos , Nervio Mandibular/fisiopatología , Síndromes de Compresión Nerviosa/etiología , Traumatismos del Nervio Trigémino/etiología , Mentón/inervación , Tomografía Computarizada de Haz Cónico , Implantación Dental Endoósea/instrumentación , Implantes Dentales/efectos adversos , Remoción de Dispositivos , Femenino , Humanos , Hipoestesia/etiología , Labio/inervación , Masculino , Mandíbula/diagnóstico por imagen , Mandíbula/cirugía , Nervio Mandibular/diagnóstico por imagen , Persona de Mediana Edad , Parestesia/etiología , Complicaciones PosoperatoriasRESUMEN
Pyoderma gangrenosum (PG) is a rare ulcerative neutrophilic disorder of skin. Its pulmonary manifestations are uncommon and only less than forty cases have been reported in the literature previously. PG is associated with variable systemic diseases, most commonly inflammatory bowel disease and hematologic malignancies. It reported rarely in rheumatoid arthritis (RA). We report a case of PG lung involvement in patient with RA associated interstitial lung disease. A 66-year-old woman presented with productive cough and recurrent ulcerative lesion on her left ankle. She had a 15-year history of RA associated interstitial lung disease and treated with methotrexate, sulfasalazine, hydroxychloroquine and methylprednisolone. On physical examination, there were a few tender, erythematous subcutaneous nodules ranging from 1 to 3 cm in diameter on her left thigh and left elbow. Anti-neutrophil cytoplasmic antibodies (ANCAs) are negative. Her chest CT scan demonstrated multifocal cavitary consolidations on the background of reticular opacity and honeycombing. Punch biopsy of erythematous nodule on thigh showed neutrophilic abscess with necrotic debris. The skin and lung lesions were rapidly improved with 0.5 mg/kg/day of prednisolone.
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PURPOSE: The objective of this study was to evaluate the interaction between 4-hexylresorcinol (4HR) and antibody as that affects the performance of a silk-4HR combination graft for soft tissue augmentation in an animal model. METHODS: The silk graft materials consisted of four types: silk+10% tricalcium phosphate (TCP) (ST0), silk+10% TCP+1% 4HR (ST1), silk+10% TCP+3% 4HR (ST3), and silk+10% TCP+6% 4-HR (ST6). The antibody binding assay tested the 4HR effect and scanning electron microscopic (SEM) exam was done for silk grafts. The animal experiment used a subcutaneous pocket mouse model. The graft - SH0 or SH1 or SH3 or SH6 - was placed in a subcutaneous pocket. The animals were killed at one, two, and four weeks, postoperatively. The specimens were subjected to histological analysis and lysozyme assay. RESULTS: Groups with 4HR applied showed lower antibody binding affinity to antigen compared to groups without 4HR. In the SEM examination, there was no significant difference among groups. Histological examinations revealed many foreign body giant cells in ST0 and ST1 group at four weeks postoperatively. Both ST3 and ST6 groups developed significantly lower levels of giant cell values compared to ST0 and ST1 groups (P <0.001) at four weeks postoperatively. In the lysozyme assay, the ST1 and ST3 groups showed denser signals than the other groups. CONCLUSION: 4HR combined silk implants resulted in high levels of vascular and connective tissue regeneration.
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Post-traumatic anterior open bite can occur as a result of broken balance among the masticatory muscles. The superior hyoid muscle group retracts the mandible downward and contributes to the anterior open bite. Denervation of the digastric muscle by injection of botulinum toxin type A (BTX-A) can reduce the power of the digastric muscle and help to resolve the post-traumatic anterior open bite. A patient with a bilateral angle fracture had an anterior open bite even after undergoing three operations under general anesthesia and rubber traction. Although the open bite showed some improvement by the repeated operation, the occlusion was still unstable six weeks after the initial treatment. To eliminate the residual anterior open bite, BTX-A was injected into the anterior belly of the digastric muscle. Following injection of BTX-A, the anterior open bite showed immediate improvement. Complication and relapse were not observed during follow-up. Long-standing post-traumatic open bite could be successfully corrected by injection of BTX-A into the anterior belly of the digastric muscle without complication.
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Many bioactive molecules like recombinant human bone morphogenetic protein 2 (rhBMP-2) have been developed for mineralized bone grafts, for which proper scaffolds are necessary to successfully apply the bioactive molecules. In this study, we tested the osteogenic efficacy of rhBMP-2 produced in-house in combination with gelatin sponge as the scaffold carrier in a rabbit radial defect model. The efficacy of the rhBMP-2 was determined by alkaline phosphatase activity assay of C2C12 cells. Two groups of ten rabbits each were treated with rhBMP-2/gelatin sponge, or gelatin sponge only. At 4 weeks, rhBMP-2/gelatin sponge grafts showed more bone regeneration than gelatin sponge grafts, as determined by X-ray radiography, micro-computed tomography, and histological analyses. At 8 weeks, rhBMP-2/gelatin sponge grafts exerted much stronger osteogenic effects. The study demonstrates the improved osteogenic efficacy of the rhBMP-2/gelatin sponge grafts in a rabbit radial bone defect model acting as a bone-inductive material.
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Proteína Morfogenética Ósea 2/farmacología , Regeneración Ósea/efectos de los fármacos , Gelatina/química , Radio (Anatomía)/patología , Factor de Crecimiento Transformador beta/farmacología , Fosfatasa Alcalina/metabolismo , Animales , Línea Celular , Subunidad alfa 1 del Factor de Unión al Sitio Principal/metabolismo , Humanos , Ratones , Modelos Animales , Conejos , Radio (Anatomía)/diagnóstico por imagen , Proteínas Recombinantes/farmacología , Proteínas Smad/metabolismo , Tomografía Computarizada por Rayos XRESUMEN
An acetonitrile solution of mercury(II) chloride has been found to catalyze efficiently the conversion of a diverse range of ketoximes into their corresponding amides/lactams.