Asunto(s)
Detección Precoz del Cáncer/métodos , Servicio de Urgencia en Hospital , Infecciones por Papillomavirus/prevención & control , Vacunas contra Papillomavirus/uso terapéutico , Cooperación del Paciente , Neoplasias del Cuello Uterino/diagnóstico , Adolescente , Adulto , Femenino , Hospitales Urbanos , Humanos , Prueba de Papanicolaou , Proyectos Piloto , Estudios Prospectivos , Neoplasias del Cuello Uterino/prevención & control , Frotis Vaginal , Adulto JovenRESUMEN
BACKGROUND: Homocysteine is an essential amino acid, whose clinical utilization includes detection of homocystinuria, vitamin B12 and folate deficiencies and cardiovascular disease management. We report assay performance of the Catch reagents on the Hitachi 917 chemistry analyzer. METHODS: Linearity, recovery, imprecision and interference from lipids, bilirubin, hemoglobin and ascorbic acid were determined on a Hitachi 917 chemistry analyzer. Split sample aliquots were assayed using the described method, by HPLC and fluorescence polarization immunoassay for method comparisons. RESULTS: The assay was linear to at least 45 micromol/l. Intra- and interassay variation ranged from 1.7% to 3.4% and 3.3% to 6.7%, respectively. Interference was observed when hemoglobin concentrations was >7 g/l and intralipid >5 g/l. No interference was observed from bilirubin or ascorbic acid. The assay was in good agreement with values obtained by HPLC and fluorescence polarization immunoassay. CONCLUSIONS: The performance of the Catch homocysteine assay was acceptable on the Hitachi 917 chemistry analyzer.