Recent Advances in Management of Neuropathic, Nociceptive, and Chronic Pain: A Narrative Review with Focus on Nanomedicine, Gene Therapy, Stem Cell Therapy, and Newer Therapeutic Options.

PURPOSE OF REVIEW: This manuscript summarizes novel clinical and interventional approaches in the management of chronic, nociceptive, and neuropathic pain. RECENT FINDINGS: Pain can be defined as a feeling of physical or emotional distress caused by an external stimulus. Pain can be grouped into distinct types according to characteristics including neuropathic pain, which is a pain caused by disease or lesion in the sensory nervous system; nociceptive pain, which is pain that can be sharp, aching, or throbbing and is caused by injury to bodily tissues; and chronic pain, which is long lasting or persisting beyond 6 months. With improved understanding of different signaling systems for pain in recent years, there has been an upscale of methods of analgesia to counteract these pathological processes. Novel treatment methods such as use of cannabinoids, stem cells, gene therapy, nanoparticles, monoclonal antibodies, and platelet-rich plasma have played a significant role in improved strategies for therapeutic interventions. Although many management options appear to be promising, extensive additional clinical research is warranted to determine best practice strategies in the future for clinicians.

Remediation of potentially toxic elements -containing wastewaters using water hyacinth - a review.

For a long time, water hyacinth has been considered a very stubborn and troublesome weed. However, research has shown that it can be used to remove many pollutants from water. Among the different pollutants, potentially toxic elements (PTE) or their ions have been found to be very toxic for humans, animals, and plants. Among the many conventional methods for removing PTE from wastewaters, phytoremediation has several advantages. This method is highly eco-friendly, cost-effective, and can remove a wide range of metal pollutants and organic pollutants. Both, living and non-living water hyacinth plants, can be used for remediation - either entirely or their parts. Study on mechanisms and different factors involved in the process would help to effectively use water hyacinth for remediation. This review presents different studies conducted in the past thirty years for the removal of PTEs. Detailed analysis of the work done in this field showed that in spite of the main advantages provided by the plant, not much has been done to increase the efficiency of the remediation process and for reusing the water hyacinth biomass for other applications after desorption of the PTE. Hence, the section on scope for future work highlights these prospective ideas. Novelty statement: Water hyacinth, which is a very stubborn weed and has a negative impact on the environment, can be constructively used to remove potentially toxic elements (PTEs) along with other pollutants from wastewaters. Different parts of the water hyacinth plant like roots, leaves, and stems or the entire plant can be used. Further, either the live plant or its other forms, such as dried powder, biochar, or activated carbon can be used. This review focuses on different forms of water hyacinth plant used, the advantages and limitations associated with these methods and the scope for future work.

Prospective analysis of laparoscopic versus open radical nephrectomy for renal tumours more than 7 cm.

AIMS: To analyse the feasibility of laparoscopic radical nephrectomy (LRN) for renal tumours >7 cm and to compare the operative and oncologic outcomes with open radical nephrectomy (ORN). SETTINGS AND DESIGN: This was a prospective, observational, comparative study. SUBJECTS AND METHODS: The study was conducted at a tertiary care super-speciality hospital. All the patients who underwent radical nephrectomy for >7 cm renal tumours during a period of 2 years (April 2012 to May 2014) were included in the study. Thirty patients were included in each ORN and LRN group. Pre-operative, intra-operative and post-operative data for all these patients were collected and analysed. STATISTICAL ANALYSIS USED: Statistical Package for the Social Sciences (SPSS, version 11.0 for Windows, Chicago, IL). RESULTS: Mean age of patients in ORN and LRN groups was 57.3 ± 6.1 years and 54.9 ± 5.7 years, respectively (P = 0.220). As compared to ORN, LRN had less drop in post-operative haemoglobin (1.39 ± 0.55 g/dl vs. 4.07 ± 1.023 g/dl, P < 0.05), less drop in haematocrit value (4.7 ± 3.25% vs. 9.5 ± 5.13%, P < 0.05), less analgesic requirement for tramadol hydrochloride (165 ± 74.5 mg vs. 260 ± 181.66 mg) and less mean hospital stay (4.2 days vs. 6.1 days, P < 0.05). There was no statistically significant difference in post-operative complication rate and recurrence-free survival over a median follow-up of 17 months (93.9% - LRN vs. 90% - ORN). CONCLUSIONS: LRN for large renal tumours is feasible and achieves oncologic outcomes similar to that obtained with ORN.

Synthesis of polycarbonate-r-polyethylene glycol copolymer for templated synthesis of mesoporous TiO2 films.

We synthesized a novel polycarbonate Z-r-polyethylene glycol (PCZ-r-PEG) copolymer by solution polycondensation. Successful synthesis of PCZ-r-PEG copolymer was confirmed by Fourier transform infrared spectroscopy (FTIR), nuclear magnetic resonance (1H-NMR), gel permeation chromatography (GPC), and transmission electron microscopy (TEM). PCZ-r-PEG copolymer was used as a structure-directing agent for fabrication of mesoporous thin film containing a titanium dioxide (TiO2) layer. To control the porosity of the resultant inorganic layer, the ratio of titanium(IV) isopropoxide (TTIP) to PCZ-r-PEG copolymer was varied. The structure and porosity of the resulting mesoporous films were characterized by X-ray diffraction (XRD) and scanning electron microscopy (SEM) analyses. Mesoporous TiO2 films fabricated on an F-doped tin oxide (FTO) surface were used as photoanodes for quasi-solid-state dye-sensitized solar cells (qssDSSCs). The highest efficiency achieved was 3.3% at 100 mW/cm2 for a film thickness of 750 nm, which is high considering the thickness of TiO2 film, indicating the importance of the structure-directing agent.

Biopolymer-Based Biomimetic Aerogel for Biomedical Applications.

Aerogels are lightweight and highly porous materials that have been found to have great potential in biomedical research because of some of their unique properties, such as their high surface area, tunable porosity, and biocompatibility. Researchers have been exploring ways to use aerogels to create biomimetic scaffolds inspired by natural extracellular matrices (ECMs) for various biomedical applications. Aerogel scaffolds can serve as three-dimensional (3D) templates for cell growth and tissue regeneration, promoting wound healing and tissue repair. Additionally, aerogel-based scaffolds have great potential in controlled drug delivery systems, where their high surface area and porosity enable the efficient loading and release of therapeutic agents. In this review, we discuss biopolymer-based biomimetic aerogel scaffolds for tissue engineering, drug delivery, and biosensors. Finally, we also discuss the potential directions in the development of aerogel-based biomimetic scaffolds.

Tissue adhesives based on chitosan for biomedical applications.

Chitosan bio-adhesives bond strongly with various biological tissues, such as skin, mucosa, and internal organs. Their adhesive ability arises from amino acid and hydroxyl groups in chitosan, facilitating interactions with tissue surfaces through chemical (ionic, covalent, and hydrogen) and physical (chain entanglement) bonding. As non-toxic, biodegradable, and biocompatible materials, chitosan bio-adhesives are a safe option for medical therapies. They are particularly suitable for drug delivery, wound healing, and tissue regeneration. In this review, we address chitosan-based bio-adhesives and the mechanisms associated with them. We also discuss different chitosan composite-based bio-adhesives and their biomedical applications in wound healing, drug delivery, hemostasis, and tissue regeneration. Finally, challenges and future perspectives for the clinical use of chitosan-based bio-adhesives are discussed.

Oxidative stress modulating nanomaterials and their biochemical roles in nanomedicine.

Many pathological conditions are predominantly associated with oxidative stress, arising from reactive oxygen species (ROS); therefore, the modulation of redox activities has been a key strategy to restore normal tissue functions. Current approaches involve establishing a favorable cellular redox environment through the administration of therapeutic drugs and redox-active nanomaterials (RANs). In particular, RANs not only provide a stable and reliable means of therapeutic delivery but also possess the capacity to finely tune various interconnected components, including radicals, enzymes, proteins, transcription factors, and metabolites. Here, we discuss the roles that engineered RANs play in a spectrum of pathological conditions, such as cancer, neurodegenerative diseases, infections, and inflammation. We visualize the dual functions of RANs as both generator and scavenger of ROS, emphasizing their profound impact on diverse cellular functions. The focus of this review is solely on inorganic redox-active nanomaterials (inorganic RANs). Additionally, we deliberate on the challenges associated with current RANs-based approaches and propose potential research directions for their future clinical translation.

Production and Bioconversion Efficiency of Enzyme Membrane Bioreactors in the Synthesis of Valuable Products.

The demand for bioactive molecules with nutritional benefits and pharmaceutically important properties is increasing, leading researchers to develop modified production strategies with low-cost purification processes. Recent developments in bioreactor technology can aid in the production of valuable products. Enzyme membrane bioreactors (EMRs) are emerging as sustainable synthesis processes in various agro-food industries, biofuel applications, and waste management processes. EMRs are modified reactors used for chemical reactions and product separation, particularly large-molecule hydrolysis and the conversion of macromolecules. EMRs generally produce low-molecular-weight carbohydrates, such as oligosaccharides, fructooligosaccharides, and gentiooligosaccharides. In this review, we provide a comprehensive overview of the use of EMRs for the production of valuable products, such as oligosaccharides and oligodextrans, and we discuss their application in the bioconversion of inulin, lignin, and sugars. Furthermore, we critically summarize the application and limitations of EMRs. This review provides important insights that can aid in the production of valuable products by food and pharmaceutical industries, and it is intended to assist scientists in developing improved quality and environmentally friendly prebiotics using EMRs.

Electrospinning Nanofibers as a Dressing to Treat Diabetic Wounds.

Globally, diabetic mellitus (DM) is a common metabolic disease that effectively inhibits insulin production, destroys pancreatic ß cells, and consequently, promotes hyperglycemia. This disease causes complications, including slowed wound healing, risk of infection in wound areas, and development of chronic wounds all of which are significant sources of mortality. With an increasing number of people diagnosed with DM, the current method of wound healing does not meet the needs of patients with diabetes. The lack of antibacterial ability and the inability to sustainably deliver necessary factors to wound areas limit its use. To overcome this, a new method of creating wound dressings for diabetic patients was developed using an electrospinning methodology. The nanofiber membrane mimics the extracellular matrix with its unique structure and functionality, owing to which it can store and deliver active substances that greatly aid in diabetic wound healing. In this review, we discuss several polymers used to create nanofiber membranes and their effectiveness in the treatment of diabetic wounds.

Recent Development in Novel Lithium-Sulfur Nanofiber Separators: A Review of the Latest Fabrication and Performance Optimizations.

Lithium-Sulfur batteries (LSBs) are one of the most promising next-generation batteries to replace Li-ion batteries that power everything from small portable devices to large electric vehicles. LSBs boast a nearly five times higher theoretical capacity than Li-ion batteries due to sulfur's high theoretical capacity, and LSBs use abundant sulfur instead of rare metals as their cathodes. In order to make LSBs commercially viable, an LSB's separator must permit fast Li-ion diffusion while suppressing the migration of soluble lithium polysulfides (LiPSs). Polyolefin separators (commonly used in Li-ion batteries) fail to block LiPSs, have low thermal stability, poor mechanical strength, and weak electrolyte affinity. Novel nanofiber (NF) separators address the aforementioned shortcomings of polyolefin separators with intrinsically superior properties. Moreover, NF separators can easily be produced in large volumes, fine-tuned via facile electrospinning techniques, and modified with various additives. This review discusses the design principles and performance of LSBs with exemplary NF separators. The benefits of using various polymers and the effects of different polymer modifications are analyzed. We also discuss the conversion of polymer NFs into carbon NFs (CNFs) and their effects on rate capability and thermal stability. Finally, common and promising modifiers for NF separators, including carbon, metal oxide, and metal-organic framework (MOF), are examined. We highlight the underlying properties of the composite NF separators that enhance the capacity, cyclability, and resilience of LSBs.

Graphene-Based Aerogels for Biomedical Application.

Aerogels are three-dimensional solid networks with incredibly low densities, high porosity, and large specific surface areas. These aerogels have both nanoscale and macroscopic interior structures. Combined with graphene, the aerogels show improved mechanical strength, electrical conductivity, surface area, and adsorption capacity, making them ideal for various biomedical applications. The graphene aerogel has a high drug-loading capacity due to its large surface area, and the porous structure enables controlled drug release over time. The presence of graphene makes it a suitable material for wound dressings, blood coagulation, and bilirubin adsorption. Additionally, graphene's conductivity can help in the electrical stimulation of cells for improved tissue regeneration, and it is also appropriate for biosensors. In this review, we discuss the preparation and advantages of graphene-based aerogels in wound dressings, drug delivery systems, bone regeneration, and biosensors.

Antibacterial activity of essential oils from different parts of plants against Salmonella and Listeria spp.

Essential oils (EOs) have recently gained popularity as natural food preservatives due to their potent antibacterial activity against food pathogens. In this review, the antibacterial activity of EOs from various plant parts and sources against the most important food pathogens Salmonella and Listeria have been discussed. The antibacterial activity of EOs is attributed to their major and minor low-molecular weight terpenes, terpenoids, phenylpropenes and aliphatic components. The major compounds along with minor components of EO extracted from different parts of various plant species were found to be responsible for antibacterial activity. The combination of EO from different sources presented synergistic anti-listerial and anti-salmonella effects. EO combined with biopolymer and in nanoemulsion form presented significant antibacterial activity. The mode of antibacterial action by EO was complex and involves a series of event that has also been discussed in detail.

Recent Development of Brain Organoids for Biomedical Application.

Over the years, scientists have studied the behavior and anatomy of many animals to understand the own species. However, despite the continuous efforts, it is often difficult to know for certain how the brain works due to the differences between the brains of animals and the human brain. While the use of animal models for research continues, the origin of human cognition and neurological disorders needs further elucidation. To that end, in vitro organoids that exhibit in vivo characteristics of the human brain have been recently developed. These brain-like organoids enable researchers to dive deeper into understanding the human brain, its neurological structures, and the causes of neurological pathologies. This paper reviews the recent developments in the regeneration of brain-like organoids using Matrigel and other alternatives. Further, gel-free methods that may enhance the regeneration process of organoids are discussed. Finally, the vascularized brain organoid growth and development in both in vitro and in vivo conditions are detailed.

Chitosan Based Biodegradable Composite for Antibacterial Food Packaging Application.

A recent focus on the development of biobased polymer packaging films has come about in response to the environmental hazards caused by petroleum-based, nonbiodegradable packaging materials. Among biopolymers, chitosan is one of the most popular due to its biocompatibility, biodegradability, antibacterial properties, and ease of use. Due to its ability to inhibit gram-negative and gram-positive bacteria, yeast, and foodborne filamentous fungi, chitosan is a suitable biopolymer for developing food packaging. However, more than the chitosan is required for active packaging. In this review, we summarize chitosan composites which show active packaging and improves food storage condition and extends its shelf life. Active compounds such as essential oils and phenolic compounds with chitosan are reviewed. Moreover, composites with polysaccharides and various nanoparticles are also summarized. This review provides valuable information for selecting a composite that enhances shelf life and other functional qualities when embedding chitosan. Furthermore, this report will provide directions for the development of novel biodegradable food packaging materials.

Water-stable, biocompatible, and highly luminescent perovskite nanocrystals-embedded fiber-based paper for anti-counterfeiting applications.

In this study, we present a promising and facile approach toward the fabrication of non-toxic, water-stable, and eco-friendly luminescent fiber paper composed of polycaprolactone (PCL) polymer and CsPbBr3@SiO2 core-shell perovskite nanocrystals. PCL-perovskite fiber paper was fabricated using a conventional electrospinning process. Transmission electron microscopy (TEM) clearly revealed incorporation of CsPbBr3@SiO2 nanocrystals in the fibers, while scanning electron microscopy (SEM) demonstrated that incorporation of CsPbBr3@SiO2 nanocrystals did not affect the surface and diameter of the PCL-perovskite fibers. In addition, thermogravimetric analysis (TGA) and contact angle measurements have demonstrated that the PCL-perovskite fibers exhibit excellent thermal and water stability. The fabricated PCL-perovskite fiber paper exhibited a bright green emission centered at 520 nm upon excitation by ultra-violet (UV) light (374 nm). We have demonstrated that fluorescent PCL-perovskite fiber paper is a promising candidate for anti-counterfeiting applications because various patterns can be printed on the paper, which only become visible after exposure to UV light at 365 nm. Cell proliferation tests revealed that the PCL-perovskite fibers are cytocompatibility. Consequently, they may be suitable for biocompatible anti-counterfeiting. The present study reveals that PCL-perovskite fibers may pave way toward next generation biomedical probe and anti-counterfeiting applications.

Conducting polymer-based scaffolds for neuronal tissue engineering.

Neuronal tissue engineering has immense potential for treating neurological disorders and facilitating nerve regeneration. Conducting polymers (CPs) have emerged as a promising class of materials owing to their unique electrical conductivity and biocompatibility. CPs, such as poly(3,4-ethylenedioxythiophene) (PEDOT), poly(3-hexylthiophene) (P3HT), polypyrrole (PPy), and polyaniline (PANi), have been extensively explored for their ability to provide electrical cues to neural cells. These polymers are widely used in various forms, including porous scaffolds, hydrogels, and nanofibers, and offer an ideal platform for promoting cell adhesion, differentiation, and axonal outgrowth. CP-based scaffolds can also serve as drug delivery systems, enabling localized and controlled release of neurotrophic factors and therapeutic agents to enhance neural regeneration and repair. CP-based scaffolds have demonstrated improved neural regeneration, both in vitro and in vivo, for treating spinal cord and peripheral nerve injuries. In this review, we discuss synthesis and scaffold processing methods for CPs and their applications in neuronal tissue regeneration. We focused on a detailed literature review of the central and peripheral nervous systems.

Tubular nanomaterials for bone tissue engineering.

Nanomaterial composition, morphology, and mechanical performance are critical parameters for tissue engineering. Within this rapidly expanding space, tubular nanomaterials (TNs), including carbon nanotubes (CNTs), titanium oxide nanotubes (TNTs), halloysite nanotubes (HNTs), silica nanotubes (SiNTs), and hydroxyapatite nanotubes (HANTs) have shown significant potential across a broad range of applications due to their high surface area, versatile surface chemistry, well-defined mechanical properties, excellent biocompatibility, and monodispersity. These include drug delivery vectors, imaging contrast agents, and scaffolds for bone tissue engineering. This review is centered on the recent developments in TN-based biomaterials for structural tissue engineering, with a strong focus on bone tissue regeneration. It includes a detailed literature review on TN-based orthopedic coatings for metallic implants and composite scaffolds to enhance in vivo bone regeneration.

Wastewater Treatment by Polymeric Microspheres: A Review.

This review addresses polymer microspheres used as adsorbent for wastewater treatment. The removal of various pollutants (including dyes, heavy metal ions, and organic pollutants) is a prominent issue, as they can cause severe health problems. Porous microspheres can provide large specific area and active sites for adsorption or photo degradation. Enhancement in performance is achieved by various modifications, such as the introduction of nanoparticles, magnetic particles, and ZIF-8. Some microspheres were synthesized from synthetic polymers such as vinylic polymer and polydopamine (PDA) through a facile fabrication process. Natural polymers (such as cellulose, alginate, and chitosan) that are biodegradable and eco-friendly are also used. The adsorbents used in industrial application require high adsorption capacity, thermal stability, and recyclability. Batch adsorption experiments were conducted to investigate the optimal conditions, influence of related factors, and adsorption capacities. Insights regarding the adsorption mechanisms were given from the kinetic model, isotherm model, and various characterization methods. The recyclability is investigated through regeneration ratio, or their maintenance of their capability through repeated adsorption-desorption cycles. The high potential of polymer microsphere for the removal of pollutants from wastewater is shown through the high adsorption capacities, environmentally friendliness, and high stability.

Dual-Color Emission from Spatially Distributed Quantum Dots in Poly(l-lactide) Films with Diverse Morphologies.

Polymer-based multicolor emissive materials have growing demand due to their potential applications in various fields such as full-color displays, bioimaging, and light sources because of their processability and high stability. Herein, we report dual-color emissive hybrid materials based on biocompatible poly(l-lactide) and polyethylene glycol-modified two-dimensional layered double hydroxide quantum dots (PEG-LDHQDs). The morphology of polymer films tunes the spatial distribution of QDs within the polymer matrix, modulating the energy transfer between the QDs and affording the dual emission behavior in the aggregated states. The amorphous hybrid films show single emission (blue) from the finely dispersed QDs (mostly isolated) within the polymer matrix. In contrast, dual emission (blue and red) was observed when the polymer was crystallized due to the possible accumulation of QDs at the interface of crystalline and amorphous phases in the lamellar structure. Furthermore, the dual emission could be enhanced by the aggregation of QDs on the pores of the breath figure pattern constructed on the surface of the hybrid film.

Modification strategies of membranes with enhanced Anti-biofouling properties for wastewater Treatment: A review.

This review addresses composite membranes used for wastewater treatment, focusing heavily on the anti-biofouling properties of such membranes. Biofouling caused by the development of a thick biofilm on the membrane surface is a major issue that reduces water permeance and reduces its lifetime. Biofilm formation and adhesion are mitigated by modifying membranes with two-dimensional or zero-dimensional carbon-based nanomaterials or their modified substituents. In particular, nanomaterials based on graphene, including graphene oxide and carbon quantum dots, are mainly used as nanofillers in the membrane. Functionalization of the nanofillers with various organic ligands or compositing the nanofiller with other materials, such as silver nanoparticles, enhances the bactericidal ability of composite membranes. Moreover, such membrane modifications reduce biofilm adhesion while increasing water permeance and salt/dye rejection. This review discusses the recent literature on developing graphene oxide-based and carbon quantum dot-based composite membranes for biofouling-resistant wastewater treatment.