A comprehensive analysis of transcriptomic data for comparison of cold tolerance in two Brassica napus genotypes.

Brassica napus is an important oil crop and cold stress severely limits its productivity. To date, several studies have reported the regulatory genes and pathways involved in cold-stress responses in B. napus. However, transcriptome-scale identification of the regulatory genes is still lacking. In this study, we performed comparative transcriptome analysis of cold-tolerant C18 (CT - C18) and cold-sensitive C6 (CS - C6) Brassica napus genotypes under cold stress for 7 days, with the primary purpose of identifying cold-responsive transcription in B. napus. A total of 6061 TFs belonging to 58 families were annotated in the B. napus genome, of which 3870 were expressed under cold stress in both genotypes. Among these, 451 TFs were differentially expressed (DE), with 21 TF genes expressed in both genotypes. Most TF members of the MYB (26), bHLH (23), and NAC (17) families were significantly expressed in the CT - C18 genotype compared with the CS - C6 B. napus genotype. GO classification showed a significant role in transcription regulation, DNA-binding transcription factor activity, response to chitin, and the ethylene-activated signaling pathway. KEGG pathway annotation revealed these TFs are involved in regulating more pathways, resulting in more tolerance. In conclusion, the results provide insights into the molecular regulation mechanisms of B. napus in response to freezing treatment, expanding our understanding of the complex molecular mechanisms in plants' response to freezing stress.

Double-function enhancement algorithm for low-illumination images based on retinex theory.

In order to solve the problems of noise amplification and excessive enhancement caused by low contrast and uneven illumination in the process of low-illumination image enhancement, a high-quality image enhancement algorithm is proposed in this paper. First, the total-variation model is used to obtain the smoothed V- and S-channel images, and the adaptive gamma transform is used to enhance the details of the smoothed V-channel image. Then, on this basis, the improved multi-scale retinex algorithms based on the logarithmic function and on the hyperbolic tangent function, respectively, are used to obtain different V-channel enhanced images, and the two images are fused according to the local intensity amplitude of the images. Finally, the three-dimensional gamma function is used to correct the fused image, and then adjust the image saturation. A lightness-order-error (LOE) approach is used to measure the naturalness of the enhanced image. The experimental results show that compared with other classical algorithms, the LOE value of the proposed algorithm can be reduced by 79.95% at most. Compared with other state-of-the-art algorithms, the LOE value can be reduced by 53.43% at most. Compared with some algorithms based on deep learning, the LOE value can be reduced by 52.13% at most. The algorithm proposed in this paper can effectively reduce image noise, retain image details, avoid excessive image enhancement, and obtain a better visual effect while ensuring the enhancement effect.

The role of strigolactone analog (GR24) in endogenous hormone metabolism and hormone-related gene expression in tobacco axillary buds.

KEY MESSAGE: Strigolactone has the potential to influence hormone metabolism, in addition to having a role in inhibiting axillary bud elongation, which could be regulated by the expression of phytohormones-related genes. The elongation of axillary buds affects the economic benefits of tobacco. In this study, it was investigated the effect of strigolactone (SL) on the elongation of tobacco axillary buds and its endogenous hormone metabolism and related gene expression by applying the artificial analog of SL, GR24, and an inhibitor of SL synthesis, TIS-108, to the axillary buds. The results showed that the elongation of axillary buds was significantly inhibited by GR24 on day 2 and day 9. Ultra-high-performance liquid-chromatography-mass spectrometry results further showed that SL significantly affected the metabolism of endogenous plant hormones, altering both their levels and the ratios between each endogenous hormone. Particularly, the levels of auxin (IAA), trans-zeatin-riboside (tZR), N6-(∆2-isopentenyl) adenine (iP), gibberellin A4 (GA4), jasmonic acid (JA), and jasmonoyl isoleucine (JA-Ile) were decreased after GR24 treatment on day 9, but the levels of 1-aminocyclopropane-1-carboxylic acid (ACC) and gibberellin A1 (GA1) were significantly increased. Further analysis of endogenous hormonal balance revealed that after the treatment with GR24 on day 9, the ratio of IAA to cytokinin (CTK) was markedly increased, but the ratios of IAA to abscisic acid (ABA), salicylic acid (SA), ACC, JAs, and, GAs were notably decreased. In addition, according to RNA-seq analysis, multiple differentially expressed genes were found, such as GH3.1, AUX/IAA, SUAR20, IPT, CKX1, GA2ox1, ACO3, ERF1, PR1, and HCT, which may play critical roles in the biosynthesis, deactivation, signaling pathway of phytohormones, and the biosynthesis of flavonoids to regulate the elongation of axillary buds in tobacco. This work lays the certain theoretical foundation for the application of SL in regulating the elongation of axillary buds of tobacco.

Analysis of image motion for optical remote sensing satellites staring imaging with area-array detectors.

In this paper, the image motion introduced by the staring action itself during optical remote sensing satellites staring imaging with area-array detectors is studied. The image motion is decomposed into the angle-rotation image motion caused by the change of observation angle, the size-scaling image motion caused by the change of observation distance, and the Earth-rotation image motion caused by the rotation of the ground object with the Earth. The theoretical derivation of the angle-rotation image motion and size-scaling image motion is conducted, and the numerical analysis of the Earth-rotation image motion is carried out. Based on the comparison among the characteristics of the three types of image motions, the conclusion is drawn that, for general staring imaging scenes, the angle-rotation image motion is dominant, followed by the size-scaling image motion and the ignorable Earth-rotation image motion. On the condition that the image motion does not exceed 1 pixel, the allowed maximum exposure time for area-array staring imaging is analyzed. It is found that the large-array satellite is not suitable for long-exposure imaging, as its allowed exposure time decreases rapidly with the increase of roll angle. A satellite with a 12k×12k area-array detector and 500 km orbit is taken as an example. The allowed exposure time is 0.88 s when roll angle of the satellite is 0°; it decreases to 0.02 s when the roll angle increases to 28°.

Image blur analysis and elimination algorithm for an MTF test target of a space camera.

The high-resolution space camera needs to image the fringe target that meets the sampling frequency in the modulation transfer function test. However, blurry regions often appear in the target image, which seriously affects the visual effect and test efficiency. In this paper, by analyzing the imaging process of the rectangle striated target by a complementary metal-oxide-semiconductor transistor detector, a moiré fringe blurry degradation model of the target image is proposed. According to the degradation model, it is concluded that there are low blurry degradation regions in the image. Taking the low blurry degradation region as the reference information and the region similarity as the weight, the weighted spatial filtering and mean filtering are carried out to eliminate the blurry band in the target image. The experimental results show that compared with bilinear interpolation and guidance filter, this algorithm can effectively remove the image blur caused by the target periodic error and the angle error. The work in this paper provides a practical tool and theoretical support for subsequent space camera tests.

Optimal design of tilt carrier frequency computer-generated holograms to measure aspherics.

Computer-generated holograms (CGHs) provide an approach to high-precision metrology of aspherics. A CGH is designed under the trade-off among size, mapping distortion, and line spacing. This paper describes an optimal design method based on the parametric model for tilt carrier frequency CGHs placed outside the interferometer focus points. Under the condition of retaining an admissible size and a tolerable mapping distortion, the optimal design method has two advantages: (1) separating the parasitic diffraction orders to improve the contrast of the interferograms and (2) achieving the largest line spacing to minimize sensitivity to fabrication errors. This optimal design method is applicable to common concave aspherical surfaces and illustrated with CGH design examples.

Genome-Wide Investigation of Class III Peroxidase Genes in Brassica napus Reveals Their Responsiveness to Abiotic Stresses.

Brassica napus (B. napus) is susceptible to multiple abiotic stresses that can affect plant growth and development, ultimately reducing crop yields. In the past, many genes that provide tolerance to abiotic stresses have been identified and characterized. Peroxidase (POD) proteins, members of the oxidoreductase enzyme family, play a critical role in protecting plants against abiotic stresses. This study demonstrated a comprehensive investigation of the POD gene family in B. napus. As a result, a total of 109 POD genes were identified across the 19 chromosomes and classified into five distinct subgroups. Further, 44 duplicate events were identified; of these, two gene pairs were tandem and 42 were segmental. Synteny analysis revealed that segmental duplication was more prominent than tandem duplication among POD genes. Expression pattern analysis based on the RNA-seq data of B. napus indicated that BnPOD genes were expressed differently in various tissues; most of them were expressed in roots rather than in other tissues. To validate these findings, we performed RT-qPCR analysis on ten genes; these genes showed various expression levels under abiotic stresses. Our findings not only furnish valuable insights into the evolutionary dynamics of the BnPOD gene family but also serve as a foundation for subsequent investigations into the functional roles of POD genes in B. napus.

Green Synthesis of Silver Nanoparticles Using Jasminum nudiflorum Flower Extract and Their Antifungal and Antioxidant Activity.

The synthesis of metal nanomaterials is a timely topic due to their widespread use in fields such as crop protection, the environment, medicine, and engineering. Green synthesis of nanoparticles, which uses plant extracts instead of industrial chemical agents to reduce metal ions, has been developed to decrease costs, reduce pollution, and improve environmental and human health safety. In this paper, silver nanoparticles (AgNPs) were synthesized from the flower extract of Jasminum nudiflorum. The green synthesized AgNPs were characterized by UV-Vis, FTIR, XRD, SEM, and other technologies. The antifungal activity of the prepared AgNPs against Alternaria longipes was tested using the plate method, the concentration dilution method, and other methods, and the antioxidant activity of the prepared AgNPs was evaluated by DPPH and hydroxyl free scavenging methods. The results showed that AgNPs synthesized from J. nudiflorum flower extract have a face-centered cubic structure (fcc), and the average grain size of the nanoparticles is 13 nm; they are also mainly spherical in shape. Additionally, the concentration of AgNPs (ranging from 16 to 128 µg/mL) significantly inhibited the mycelial growth of A. longipes in comparison to the control. The inhibitory rate gradually increased with increasing AgNP concentration, ranging from 70.64% to 79.60% at a concentration of 128 µg/mL. The minimum inhibitory concentration was observed at 32 µg/mL. AgNPs induced overaccumulation of MDA in A. longipes, resulting in cell membrane damage and nucleic acid leakage. Moreover, the AgNPs have significant antioxidant properties, which increase with increasing concentration. The clearance rate of DPPH was 25.46 ± 0.90% when the concentration of AgNPs was 8 µg/mL, and the clearance rate of the hydroxyl radical was 28.62 ± 0.59% when the concentration of AgNPs was 128 µg/mL. Thus, the flower extract from J. nudiflorum holds potential as an environmentally friendly and green alternative for the synthesis of AgNPs, which have antifungal and antioxidant potential.